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Tongshan, China

Huang X.,Shanghai JiaoTong University | Zhao X.,Xuzhou Childrens Hospital | Lu X.,Shanghai JiaoTong University | Tian H.,Shanghai JiaoTong University | And 3 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2014

A gas chromatographic method for simultaneous determination of 50 organochlorine (OCP) and pyrethroid (PP) pesticides in Flos Chrysanthemi was established. Accelerated solvent extraction (ASE) technique was used to extract the target compounds, cleaned with alumina neutral-florisil column, and eluted by mixed solvents of ethyl acetate and hexane (15:85, v/v). Selected pesticides were identified using HP-5 and DB1701 capillary dual column and detected by electron-capture detector. Quantitative analysis was performed using an external standard by HP-5 capillary column. Results showed that recoveries were 73.4-120.1%, and the relative standard deviations (RSDs) were 1.6-12.4%. The limits of detection of the method were 0.0021-0.0069 mg/kg, and the limits of quantity were 0.0064-0.0210 mg/kg. © 2014 Elsevier B.V.

Xu S.,Xuzhou Childrens Hospital | Xue Y.,Xuzhou Childrens Hospital
Experimental and Therapeutic Medicine | Year: 2016

Pediatric or childhood obesity is the most prevalent nutritional disorder among children and adolescents worldwide. Approximately 43 million individuals are obese, 21‑24% children and adolescents are overweight, and 16‑18% of individuals have abdominal obesity. The prevalence of obesity is highest among specific ethnic groups. Obesity increases the risk of heart diseases in children and adults. Childhood obesity predisposes the individual to insulin resistance and type 2 diabetes, hypertension, hyperlipidemia, liver and kidney diseases and causes reproductive dysfunction in adults. Obesity in children is a major health concern of the developed world. The National Health and Nutrition Examination Survey has reported that the prevalence of obesity is on the increase in all the pediatric age groups, in males and females, and in various ethnic and racial groups. Factors, such as eating habits, genetics, environment, metabolism, and lifestyle play an important role in the development of obesity. Over 90% of obesity cases are idiopathic and less than 10% are associated with genetic and hormonal causes. Obesity occurs when the body consumes more calories than it burns, through overeating and underexercising. The symptoms of obesity include breathing disorders, sleep apnea, chronic obstructive pulmonary disease, certain types of cancer such as prostate, bowel, breast and uterine, coronary heart disease, diabetes (type 2 in children), depression, liver and gallbladder problems, gastro‑esophageal reflux disease, high blood pressure, high cholesterol, stroke, and joint diseases such as osteoarthritis, pain in knees and lower back. Environmental, behavioral such as consumption of convenience foods, genetic, and family factors contribute to pediatric obesity. Obesity can be countered through lower calorie consumption, weight loss and diet programs, as well as increased physical activity. A number of endogenous molecules including leptin, hypothalamic melanocortin 4 receptor, and mitochondrial uncoupling proteins, are known to affect body weight. These molecules serve as potential targets for the pharmacological manipulation of obesity. Sibutramine and orlistat are primariliy used for the treatment of adult obesity, which produces modest weight loss, of 3‑8% compared to placebo. For children and obese adolescents, metformin is used in the case of insulin resistance and hyperinsulinemia. Octreotide is used for hypothalamic obesity. Bariatric surgery is performed for the treatment of severe childhood obesity. The causes, symptoms, prevention and treatment of pediatric obesity are described in the present review. © 2016, Spandidos Publications. All rights reserved.

Fang D.-H.,Central Laboratory | Fan C.-H.,Xuzhou Childrens Hospital | Ji Q.,Xuzhou Childrens Hospital | Qi B.-X.,Intensive Care Unit | And 2 more authors.
Molecular Biology Reports | Year: 2012

Paraoxonase is an HDL-associated enzyme that plays a preventive role against oxidative stress, which is thought to contribute to cancer development. PON1 activity varies widely among individuals, which is in part related to two common nonsynonymous polymorphisms in the PON1 gene (Q192R and L55M). The polymorphisms in PON1 have been implicated in cancer risk. However, results from the studies to date have been conflicting. To clarify the association, a meta-analysis was performed for 7,073 cases and 9,520 controls from 25 published case- control studies. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the strength of the association. Significant associations between PON1-L55M but not Q192R polymorphism and total cancer were observed from all the comparisons. In stratified analyses, PON1-55M allele was a risk factor for breast cancer. Similarly, increased risk was observed for prostate cancer (OR = 1.18, 95% CI: 1.01-1.36, Pheterogeneity = 0.260) and Caucasian population (OR = 1.18, 95% CI: 1.02-1.38, Pheterogeneity = 0.1) of the LM genotype, compared with the LL genotype. For PON1-Q192R polymorphism, PON1-192R allele was a decreased risk factor for cancer in the Asian group (RR vs QQ: OR = 0.61, 95% CI: 0.38-0.98, Pheterogeneity = 0.268; QR vs QQ: OR = 0.71, 95% CI: 0.52-0.96, Pheterogeneity = 0.130; RR + QR vs QQ: OR = 0.71, 95% CI: 0.53-0.95, Pheterogeneity = 0.135). Although some modest bias could not be eliminated, this meta-analysis suggests that the PON1-55M allele is a risk factor for the development of cancer, in particular for breast cancer. Future studies with larger sample sizes are warranted to further evaluate these associations. © 2012 Springer Science+Business Media B.V.

Xu S.,Xuzhou Childrens Hospital | Xue Y.,Xuzhou Childrens Hospital
Experimental and Therapeutic Medicine | Year: 2016

The abundance of protein and its functional diversity in living systems makes it essential for virtually all life processes. Dietary protein and amino acid requirements are affected by age, body size, body composition, physiological state, and the level of energy output. The requirement for dietary protein is based on the indispensable amino acids under all conditions and under specific physiological and pathological conditions as well as the synthesis of dispensable amino acids and other nitrogen-containing compounds. Previous findings have shown the influence of early intake of proteins on the development of overweight in healthy children. The present review focused on the possible association (if any) between protein intake and later development of obesity. The aim is to benefit physicians, dieticians as well as parents to understand future consequences of incorrect dietary habits in young adolescents and encourage healthy dietary habits to avoid obesity. © 2016, Spandidos Publications. All right reserved.

Fan C.-H.,Xuzhou Childrens Hospital | Liu W.-L.,Xuzhou Central Hospital | Cao H.,Xuzhou Childrens Hospital | Wen C.,Xuzhou Childrens Hospital | And 3 more authors.
Cell Death and Disease | Year: 2013

Temozolomide (TMZ) is an alkylating agent currently used as first-line therapy for gliomas treatment due to its DNA-damaging effect. However, drug resistance occurs, preventing multi-cycle use of this chemotherapeutic agent. One of the major mechanisms of cancer drug resistance is enhanced activity of a DNA repair enzyme, O6-methylguanine-DNA-methyltransferase (MGMT), which counteracts chemotherapy-induced DNA alkylation and is a key component of chemoresistance. MGMT repairs TMZ-induced DNA lesions, O6-meG, by transferring the alkyl group from guanine to a cysteine residue. This review provides an overview of recent advances in the field, with particular emphasis on the inhibitors of MGMT and underlying mechanisms. Literature search was performed through PubMed and all relevant articles were reviewed, with particular attention to MGMT, its role in TMZ-resistant gliomas, effects of MGMT inhibitors and the underlying mechanisms. Several strategies are currently being pursued to improve the therapeutic efficacy of TMZ via inhibition of MGMT to reduce chemoresistance and improve overall survival. MGMT may be a promising target for the treatment of TMZ-resistant gliomas. © 2013 Macmillan Publishers Limited. All rights reserved.

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