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Huang P.,Shanghai JiaoTong University | Bao L.,Xuzhou Cancer Hospital | Zhang C.,Shanghai JiaoTong University | Lin J.,Shanghai JiaoTong University | And 8 more authors.
Biomaterials | Year: 2011

Multifunctional nanoprobes are designed to own various functions such as tumor targeting, imaging and selective therapy, which offer great promise for the future of cancer prevention, diagnosis, imaging and treatment. Herein, silica was applied to replace cetyltrimethylammonium bromide (CTAB) molecules on the surface of gold nanorods (GNRs) by the classic Stöber method, thus eliminating their cytotoxicity and improving their biocompatibility. Folic acid molecule was covalently anchored on the surface of GNRs with silane coupling agent. The resultant folic acid-conjugated silica-modified GNRs show highly selective targeting, enhanced radiation therapy (RT) and photo-thermal therapy (PTT) effects on MGC803 gastric cancer cells, and also exhibited strong X-ray attenuation for in vivo X-ray and computed tomography (CT) imaging. In conclusion, the as-prepared nanoprobe is a good candidate with excellent imaging and targeting ability for X-ray/CT imaging-guided targeting dual-mode enhanced RT and PTT. © 2011 Elsevier Ltd.


Huang P.,Shanghai JiaoTong University | Bao L.,Xuzhou Cancer Hospital | Yang D.,Shanghai JiaoTong University | Gao G.,Shanghai JiaoTong University | And 4 more authors.
Chemistry - An Asian Journal | Year: 2011

Bovine serum albumin (BSA)-conjugated MxSey (M=Ag, Cd, Pb, Cu) nanomaterials with different shapes and sizes were synthesized in water at room temperature by a protein-directed, solution-phase, green synthetic method. The method features very low energy consumption and nontoxic reagents with high yields of concentrated nanoparticles. The obtained bioconjugated nanoparticles have good dispersibility, bioactivity, and biocompatibility. In addition, various functional groups of protein on the surface of the nanocrystals are suitable for further biological interactions or couplings, which is very important for further biological applications. Green giants: Bovine serum albumin (BSA)-conjugated MxSey (M=Ag, Cd, Pb, Cu) nanomaterials with different shapes and sizes were synthesized in water at room temperature by the protein-directed, solution-phase, green synthesis method. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Ding M.,Xuzhou Medical College | Ding M.,Xuzhou Cancer Hospital | Liu L.,Xuzhou Medical College | Hu C.,Lianyungang First Peoples Hospital | And 3 more authors.
Chinese Journal of Cancer Research | Year: 2014

Objective: Vascular-targeted therapy is gradually becoming more appealing for patients with lung cancer. It is unclear whether vascular endothelial growth factor receptor 2 (VEGFR2) and neuropilin-1 (NRP-1) can be biomarkers for clinical treatment. We aimed to investigate the expression levels of VEGFR2 and NRP-1 in human non-small cell lung cancer (NSCLC) and their clinical significance by observing patient prognosis. Methods: VEGFR2 and NRP-1 were assessed by immunohistochemistry (IHC) in 40 patients with NSCLC and in 10 patients with benign lesions of lung; kinase insert domain receptor (KDR) and NRP-1 copy number gain (CNG) was assessed by fluorescence in situ hybridization (FISH). The distributions of overall survival (OS) and progression-free survival (PFS) were estimated using the Kaplan-Meier method and compared between groups by log-rank test. Results: Rates of positive immunostaining for VEGFR2 and NRP-1 were 58% and 55%, respectively. KDR and NRP-1 CNG (+) were detected in 32.5% and 30% of tumors, respectively. Levels of both VEGFR2 and NRP-1 in lung tumors were significantly different than in the control tissue (χ2=11.22, P=0.001; χ2=9.82, P=0.001, respectively); similar results were obtained using CNGs (χ2=4.39, P=0.036; χ2=3.95, P=0.046, respectively). Statistically significant correlations were observed with histological grade, clinical TNM stage and the lymph node status (P<0.05), but not age, gender or pathology type (P>0.05). VEGFR2 showed a strong correlation with NRP-1 (Rs=0.68, P=0.00); similar results were observed with KDR and NRP-1 CNG (Rs=0.32, P=0.04). Significant differences in OS and PFS were observed between the groups with higher VEGFR2 and NRP-1 and those with lower expression (P<0.05). Conclusions: According to these data, VEGFR2 and NRP-1 are highly expressed in NSCLC. We can conclude that they play a key role in NSCLC occurrence, development and metastasis and are associated with patient prognosis (P<0.05 for OS and PFS). This information will be beneficial for clinical anti-angiogenic treatment in NSCLC. © Chinese Journal of Cancer Research. All rights reserved.


PubMed | Xuzhou Cancer Hospital and Southern Medical University
Type: Journal Article | Journal: Oncotarget | Year: 2016

Repeated infection with high-risk HPV is a major cause for the development and metastasis of human cervical cancer, even though the mechanism of the metastasis is still not completely understood. Here, we reported that miR-218 (microRNA-218) was downregulated in cervical cancer tissues, especially in metastatic cancer tissues. We found that miR-218 expression was associated with clinicopathological characteristics of patients with cervical cancer. MiR-218 overexpression inhibited Epithelial-Mesenchymal Transition (EMT), migration and invasiveness of cervical cancer cells in vitro. Moreover, miR-218 repressed the expression of SFMFBT1 (Scm-like with four MBT domains 1) and DCUN1D1 (defective in cullin neddylation 1, domain containing 1) by direct binding to the 3UTRs of the mRNAs. The overexpression of SFMBT1 induced EMT and increased the migration and invasiveness of cervical cancer cells, while the overexpression of DCUN1D1 increased the migration and invasiveness of these cells, but did not induce EMT. An inverse correlation was observed between the expression of miR-218 and DCUN1D1 protein in cervical cancer tissues. Importantly, HPV16 E6 downregulated the expression of miR-218 in cervical cancer, while miR-218 rescued the promotion effect of HPV16 E6 on the expression of SFMBT1 and DCUN1D1. Taken together, our results revealed that HPV16 E6 promoted EMT and invasion in cervical cancer via the repression of miR-218, while miR-218 inhibited EMT and invasion in cervical cancer by targeting SFMBT1 and DCUN1D1.


PubMed | Red Cross, Peoples Hospital of Jiangsu Province, Henan University of Science and Technology, Xuzhou Cancer Hospital and Xuzhou Medical College
Type: | Journal: Acta oto-laryngologica | Year: 2016

A prognostic model was obtained for LC. Several critical genes were unveiled. They could be potentially applied for LC recurrence prediction.Gene expression data of laryngeal cancer (LC) were analyzed to identify critical genes associated with recurrence.Two gene expression datasets were downloaded from the Gene Expression Omnibus. Dataset GSE27020 is used as the training set, containing 75 non-recurred LC cases and 34 recurred LC cases.A total of 725 DEGs were identified from the training set. A total of 4126 gene pairs showed significant correlations in non-recurred LC only, corresponding to 533 genes. A total of 7235 gene pairs showed significant correlations in recurred LC only, corresponding to 608 genes. Besides, 1694 gene pairs showed significant correlations in both non-recurred and recurred LC, corresponding to 322 genes. Functional enrichment analysis was performed for the three groups of DEGs. Seven overlapping biological functions were revealed: positive regulation of chondrocyte differentiation, autoimmune thyroid disease, focal adhesion, linoleic acid metabolism, drug metabolism, organic cation transport, and ECM-receptor interaction. Eight feature genes (PDIA3, MYH11, PDK1, SDC3, RPE65, LAMC3, BTK, and UPK1B) were identified. Their prognostic effect was validated by independent test set as well as survival analysis.


PubMed | Xuzhou Medical College, Universiti Malaysia Sabah and Xuzhou Cancer Hospital
Type: Journal Article | Journal: Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society | Year: 2016

This study is to establish a model of rat tibial osteocarcinoma pain, intrathecally inject specific ERK1/2 inhibitors SCH772984, observe the analgesic effect, and discuss the influence of ERK-P90RSK-Fos signal path in bone cancer pain. Forty female SD rats were randomly divided into 5 groups. Establish a bone cancer pain model after putting the intrathecal tube 5d and determine the rats mechanical withdrawal threshold (MWT) after tube 5d; 40 SD rats with intrathecal tube back 5d were randomly divided into 5 groups. Sham Group receives no medication, the other four respectively receive 5% DMSO 10l, SCH 0.1, 1.0, 10g (SCH dissolved in 10l 5% DMSO) intrathecally. Determine the rats mechanical withdrawal threshold (MWT) before and after giving medication 1, 3, 6, 9, 12, 15, 18, 24h, and 2min spontaneous paw withdrawal. Western blot and immuno-fluorescence determine the expression condition of spinal cord dorsal horn of p-ERK, p-p90RSK and Fos protein. Intrathecal injection of SCH772984 has analgesic effects on rats with bone cancer pain, and the effects enhance with increasing dose; intrathecal injection of SCH772984 10g could greatly reduce the expression of spinal dorsal horn Fos protein. Injecting walker 256 tumor cells into rats tibia could cause behavior changes, such as idiopathic pain sensitivity and pain; the intrathecal tube almost has no effect on motor function of rats; ERK1/2 is involved in bone cancer pain, and intrathecal injection of ERK1/2 specific inhibitors SCH772984 10g may effectively relieve bone cancer pain.


Gu A.,Xuzhou Cancer Hospital | Li X.,Xuzhou Medical College | Guo K.,Xuzhou Cancer Hospital | Ma Z.,Xuzhou Cancer Hospital
Cell Biochemistry and Biophysics | Year: 2011

Cholesystolithiasis is often associated with common bile duct stones (CBDS). In order to assess the choice of surgery in terms of effectiveness and complications in the treatment of CBDS, we have compared three surgical procedures, viz., laparoscopic choledocholithotomy T-tube drainage (LCH-TD), laparoscopic cholecystectomy with endoscopic sphincterotomy (LC-EST), and the traditional open choledocholithotomy with T-tube drainage (OCHTD). This study is a retrospective comparative analysis of LCH-TD (77 patients), LC-EST (43 patients), and OCHTD (60 patients) for CBDS. The success of the surgical procedures was assessed in terms of recovery duration, hospitalization, and post-operative complications. Both the micro-invasive procedures, LCH-TD and LC-EST, with a success rate of 92.5%, are found to be superior to the traditional OCHTD. Between the two micro-invasive procedures, patients in LCH-TD group had shorter operation time and hospital stay, and fewer post-operative complications. Although the size of the stones is comparable between these two groups, the CBD diameter was significantly larger in patients who underwent LCH-TD. In comparison to OCHTD, both LCH-TD and LC-EST are micro-invasive, safe, and suitable for routine use in patients with CBDS. Moreover, when the CBD diameter is wider than 1 cm, LCH-TD is strongly advocated. © 2010 Springer Science+Business Media, LLC.


Jiang H.,Xuzhou Cancer Hospital | Zhu M.,Xuzhou Cancer Hospital | Li Y.,Xuzhou Cancer Hospital | Li Q.,Xuzhou Cancer Hospital | And 2 more authors.
Cancer Research and Clinic | Year: 2016

Objective: To explore the relationship between mutation status of epidermal growth factor receptor (EGFR) and efficacy of EGFR tyrosine kinase inhibitor (EGFR-TKI) in patients with advanced nonsmall cell lung cancer (NSCLC). Methods: The data of 72 outpatients and inpatients with stage IIIb/IV NSCLC diagnosed by histopathology and harbored EGFR-activating mutations (exon 19 and exon 21) from January 2008 to December 2013 in Xuzhou Cancer Hospital were collected. All of them received first-line EGFR-TKI. The relationships between EGFR gene status and response rate or progression-free survival (PFS) were analyzed. Results: Of the 72 patients with EGFR mutation, 37 patients harbored exon 19 deletion, and 35 patients harbored exon 21 L858R point mutation. The efficacies of all patients were assessable. The objective response rate (ORR) was 63.9 % (46/72) and disease control rate (DCR) was 79.2 % (57/72) in all patients, including 2 cases of complete remission (CR), 44 cases of partial remission (PR), 11 cases stable disease (SD) and 15 cases of disease progression (PD). Patients with exon 19 deletion had a higher ORR [75.7 % (28/37) vs 51.4 % (18/35), P = 0.032] and a higher DCR [89.2 % (33/37) vs 68.6 % (24/35), P = 0.031] than patients with exon 21 L858R mutation. The PFS of patients with exon 19 deletion was significantly longer than that of patients with exon 21 L858R mutation (12.0 months vs 9.5 months, P = 0.030). Cox multivariate analysis indicated that the gender, histological type, smoking history were the major influence factors of PFS. The differences of toxicity between the two groups were not significant. Conclusion: EGFR-activating mutation is a predictor for PFS and ORR of first-line EGFR-TKI in patients with advanced NSCLC. © 2016, Editorial Board of Cancer Research and Clinic. All rights reserved.

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