Wang J.J.,Tongji University |
Zheng Y.,Xuhui District Central Hospital |
Yang F.,Tongji University |
Zhao P.,Shanghai University |
Li H.F.,Tongji University
International Journal of Gynecological Cancer | Year: 2010
Introduction: Small interfering RNA (siRNA) has been used to knock down the expression of specific genes. However, its delivery remains a challenge. Recently, ultrasound combined with microbubbles has been used to deliver plasmid into cells and has shown much superiority. Whether a survivin siRNA transfected with a microbubble contrast agent combined with ultrasound exposure could inhibit survivin expression and induce ovarian cancer cell apoptosis was investigated. Methods: The survivin gene was amplified and inserted into vector pEGFP-N1, resulting in psurvivin-EGFP. Three siRNA expression cassettes (SECs) targeting survivin were obtained by 2-step polymerase chain reaction. The SECs and psurvivin-EGFP were cotrans-fected into 293T cells. A functional SEC was selected and inserted into pMD18T, resulting in the survivin-targeting siRNA expression plasmid, which was transfected into SKOV-3 ovarian carcinoma cells using SonoVue, a microbubble contrast agent, and ultrasound exposure. Survivin expression was monitored by Western blot, and apoptosis was determined by fluorescence-activated cell sorting. Results: One siRNA effectively inhibited survivin expression. SonoVue with ultrasound effectively delivered survivin siRNA to SKOV-3 cells, inhibited survivin expression, and induced apoptosis. Conclusions: Delivery of survivin siRNA using a microbubble contrast agent combined with ultrasound exposure can effectively inhibit survivin expression and induce apoptosis, providing a new promising approach for siRNA delivery in vivo.
Cao M.,Shanghai University |
Zhou Z.-W.,Xuhui District Central Hospital |
Fang B.-J.,Shanghai University |
Zhao C.-G.,Shanghai University |
Zhou D.,Shanghai University
Medicine (United States) | Year: 2014
A number of studies have been conducted to explore the association between the cholesteryl ester transfer protein (CETP) TaqIB polymorphism and risk of myocardial infarction (MI); however, the results are inconsistent. Therefore, we conducted this meta-analysis to clarify the issue based on all the data available. Eligible studies were retrieved by searching PubMed,Embase,Web of Science, and Google Scholar. We calculated the crude odds ratios (ORs) and corresponding 95% confidence intervals (95% CIs) to assess the association between the TaqIB polymorphism and risk of MI. We included 13 studies involving 8733 MI cases and 8573 controls in the meta-analysis. The pooled results from all included studies showed decreased MI risk in the analysis of the B2B2 versus B1B1 (OR=0.78, 95% CI=0.680.91), dominant (OR=0.88, 95% CI=0.770.99), and recessive genetic models (OR=0.84, 95% CI=0.780.91). The frequency of the B2B2 genotype in MI patients was lower (OR=0.87, 95% CI=0.810.94). However, there was no significant association in the B1B2 versus B1B1 analysis (OR=0.92, 95% CI=0.811.05) and no significant difference for the B1B1 genotype (OR=1.04, 95% CI=0.981.11) and B1B2 genotype (OR=1.03, 95% CI=0.97 1.08). Cumulative analysis confirmed these results. Our results suggest that the B2B2 genotype of the CETP TaqIB polymorphism is a protective factor against the development of MI.
Zhang N.,Xuhui District Central Hospital |
Wang X.-H.,Putuo District Central Hospital |
Mao S.L.,Xuhui District Central Hospital |
Zhao F.,Changhai Hospital
Molecules | Year: 2011
The prevalence of metabolic syndrome has increased in modern society and the condition is proving to be a common precursor of cardiovascular disease. The aim of the present study was to investigate whether astragaloside IV, a major active constituent of Astragalus membranaceus (Fisch) Bge., is able to prevent the development of hypertension and endothelial dysfunction in fructose-fed rats. Rats were fed with 10% fructose in their drinking water for 8 weeks. From the beginning of week 5, two groups of fructose-fed rats were treated with 0.5 or 2 mg/kg, i.p., astragaloside IV. Another group of fructose-fed rats, injected with the same volume of vehicle (dimethylsulfoxide, DMSO) from week 5, served as the control group. At the end of the treatment period, blood pressure, blood glucose, glucose tolerance, blood insulin and lipids were determined. In addition, in vitro experiments were conducted at the end of the eight week treatment period to evaluate endothelium-dependent aortic vasorelaxation, as well as myocardial and aortic tissue levels of nitrate and nitrite (NOx) and cGMP. Fructose-fed rats developed clustering signs of metabolic syndrome, such as increased bodyweight, mild hypertension, hyperinsulinaemia, hypertriglyceridaemia, impaired glucose tolerance and impaired endothelium-dependent vasorelaxation. Administration of astragaloside IV reduced blood pressure and triglyceride levels in fructose-fed rats and high dose of astragaloside IV also improved glucose tolerance and endothelium-dependent vasorelaxation. The astragaloside IV-inducedimprovement in vasorelaxation was associated with increased levels of aortic NOx and cGMP and was abrogated by blockade of nitric oxide synthase with NG-nitro-l-arginine methyl ester (l-NAME). On the basis of its favourable effects on lipid metabolism, endothelium-dependent vasorelaxation and the nitric oxide-cGMP-related pathway, astragaloside IV may be useful in ameliorating food-induced metabolic syndrome. © 2011 by the authors.
Lu T.,Xuhui District Central Hospital |
Lu T.,Jiangsu University |
Sheng H.,Xuhui District Central Hospital |
Wu J.,CAS Shanghai Institutes for Biological Sciences |
And 3 more authors.
Nutrition Research | Year: 2012
For thousands of years, cinnamon has been used as a traditional treatment in China. However, there are no studies to date that investigate whether cinnamon supplements are able to aid in the treatment of type 2 diabetes in Chinese subjects. We hypothesized cinnamon should be effective in improving blood glucose control in Chinese patients with type 2 diabetes. To address this hypothesis, we performed a randomized, double-blinded clinical study to analyze the effect of cinnamon extract on glycosylated hemoglobin A 1c and fasting blood glucose levels in Chinese patients with type 2 diabetes. A total of 66 patients with type 2 diabetes were recruited and randomly divided into 3 groups: placebo and low-dose and high-dose supplementation with cinnamon extract at 120 and 360 mg/d, respectively. Patients in all 3 groups took gliclazide during the entire 3 months of the study. Both hemoglobin A 1c and fasting blood glucose levels were significantly reduced in patients in the low- and high-dose groups, whereas they were not changed in the placebo group. The blood triglyceride levels were also significantly reduced in the low-dose group. The blood levels of total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and liver transaminase remained unchanged in the 3 groups. In conclusion, our study indicates that cinnamon supplementation is able to significantly improve blood glucose control in Chinese patients with type 2 diabetes. © 2012 Elsevier Inc.
Bi M.,Xuhui District Central Hospital
Chinese Journal of Gastroenterology | Year: 2011
Background: Danshensu is extracted from the dry root of Chinese herb medicine Salvia miltiorrhiza Bge. It has antioxidant properties and cytotoxic activity against many human cancers. Aims: To investigate apoptotic effect of Danshensu on human hepatocellular carcinoma cell line SMMC7721 in vitro and to elucidate its mechanism. Methods: Hepatocellular carcinoma cell line SMMC7721 was incubated with different concentrations of Danshensu (0 mg/L, 10 mg/L, 20 mg/L, 30 mg/L, 40 mg/L) for 24, 48 and 72 hours, respectively. Cell inhibition rate was assessed by MTT assay. Morphological changes were observed under transmission electron microscope. Apoptosis was assessed by Annexin V-FITC/PI double staining after SMMC7721 cells being treated with different concentrations of Danshensu (0-40 mg/L) or with 40 mg/L Danshensu for different time (0-48 hours). mRNA expression of p53 was determined by RT-PCR after SMMC7721 cells being treated with different concentrations of Danshensu (0-40 mg/L) for 24 hours. Results: Proliferation of SMMC7721 cells was obviously inhibited and apoptosis was induced by Danshensu in dose- and time-dependent manners. Characteristic apoptosis was confirmed by transmission electron microscope. mRNA expression of p53 gene enhanced with the increase of Danshensu concentration. Conclusions: Danshensu can inhibit the proliferation and induce apoptosis of SMMC7721 cells in time- and dose-dependent manners, and its mechanism may be related to the up-regulation of p53 expression.
Chen L.,Xuhui District Central Hospital |
Li Q.-Y.,Fudan University |
Shi X.-J.,Fudan University |
Mao S.-L.,Xuhui District Central Hospital |
Du Y.-L.,China Pharmaceutical University
Journal of Agricultural and Food Chemistry | Year: 2013
Fermented soybean foods have been shown to reduce incidence of diabetes and improve insulin sensitivity. 6-Hydroxydaidzein (6-HD) is a bioactive ingredient isolated from fermented soybean. In this study, we examined the effects of 6-HD on adipocyte differentiation and insulin-stimulated glucose uptake, as well as the mechanisms involved. In our experiments, 6-HD enhanced 3T3-L1 adipocyte differentiation and insulin-stimulated glucose uptake in a dosage-dependent manner. In addition, 6-HD increased peroxisome proliferator-activated receptor gamma (PPARγ) gene expression and PPARγ transcriptional activity. 6-HD increased CCAAT/enhanced binding protein alpha (C/EBPα) expression as well. Although having no effects on glucose transporter type 4 (GLUT4) gene expression, 6-HD facilitated GLUT4 protein translocation to the cell membranes. Our results indicate that 6-HD exhibited the actions of promoting adipocyte differentiation and improving insulin sensitivity by increasing the expression of C/EBPα and facilitating the translocation of GLUT4 via the activation of PPARγ, suggesting that 6-HD can be promising in diabetes management. © 2013 American Chemical Society.
Zhou Y.,Dahua Hospital |
Qiu L.,Xuhui District Central Hospital |
Xiao Q.,Dahua Hospital |
Wang Y.,Dahua Hospital |
And 4 more authors.
Clinical Biochemistry | Year: 2013
Objectives: The objective of the study is to evaluate whether plasma amino acid (AA) differences are related with obesity or diabetes. Design and methods: In 126 diabetes and 100 non-diabetes participants, the plasma concentrations of 42 (AAs) were analyzed with a liquid chromatography tandem mass spectrometry technology (LC-MS/MS). Both groups were divided into obese and lean individuals and we compared intra- and inter-group differences between the groups. Results: In obese non-diabetic participants, 19 AA plasma concentrations were different compared to lean non-diabetic individuals, from which 15 were essential AAs, whereas in the diabetic group only three AAs differed in the obese compared to the lean patients. When comparing the overall AA differences between diabetics and non-diabetics, 16 AA concentrations were enhanced and 11 AA concentrations were reduced in the diabetic patients. A multivariate linear regression analysis revealed correlations between: FBG and Cystathionine, Proline and Citrulline; HbA1c and Glycine, Proline and Sarcosine; Cholesterol and Serine, β-alanine, Proline and Cystathionine; HDL-C and β-alanine, 1-methylhistidine and Proline; and LDL-C and α-Amino n-butyric acid and Hydroxyproline. Triglycerides were related with γ-aminobutyric acid, Serine and Alanine. Fasting insulin was related with 3-methylhistidine, Asparagine, Alanine, γ-aminobutyric acid and Cystathionine. Conclusions: The concentrations of 19 plasma AAs differed between non-diabetic obese and lean individuals, which were mostly superimposed by diabetes. Between diabetic and non-diabetic participants plasma AA concentration differences were obvious and some of these alterations were correlated to other factors like blood glucose, lipids, insulin and hemoglobin status. © 2013.
Liu L.,Fudan University |
Wu N.,Xuhui District Central Hospital |
Li J.,Fudan University |
Li J.,Shanghai Medical College
Journal of Hematology and Oncology | Year: 2012
Contemporary advancements have had little impact on the treatment of gastric cancer (GC), the worlds second highest cause of cancer death. Agents targeting human epidermal growth factor receptor mediated pathways have been a common topic of contemporary cancer research, including monoclonal antibodies (mAbs) and receptor tyrosine kinase inhibitors (TKIs). Trastuzumab is the first target agent evidencing improvements in overall survival in HER2-positive (human epidermal growth factor receptor 2) gastric cancer patients. Agents targeting vascular epithelial growth factor (VEGF), mammalian target of rapamycin (mTOR), and other biological pathways are also undergoing clinical trials, with some marginally positive results. Effective targeted therapy requires patient selection based on predictive molecular biomarkers. Most phase III clinical trials are carried out without patient selection; therefore, it is hard to achieve personalized treatment and to monitor patient outcome individually. The trend for future clinical trials requires patient selection methods based on current understanding of GC biology with the application of biomarkers. © 2012 Liu et al.; licensee BioMed Central Ltd.
Bao X.-M.,Xuhui District Central Hospital |
Zheng H.,Xuhui District Central Hospital
Clinical and Experimental Pharmacology and Physiology | Year: 2015
Statins have been reported to have an antioxidant effect against homocysteine (Hcy)-induced endothelial dysfunction. It is unknown whether they have the same effect against migration of vascular smooth muscle cells (VSMCs) induced by Hcy. In this study, it was investigated whether and how atorvastatin could inhibit the Hcy-induced migration in cultured VSMCs and revealed the possible redox mechanism. VSMCs were isolated from the thoracic aortas of Sprague-Dawley rats. The migration of VSMCs was examined using a transwell technique and cell viability was determined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazoliumbromide (MTT) assay. Reactive oxygen species (ROS) were measured using the fluoroprobe 2′7′-dichlorodihydrofluorescein diacetate. The activity of NADPH oxidase was assessed by lucigenin enhanced chemiluminescence. Expressions of Nox1 mRNA and p-p38MAPK protein were measured by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. The results showed that atorvastatin inhibited the migration of VSMCs induced by Hcy, which was reversed by the mevalonate. In addition, pretreatment with the NADPH oxidase inhibitor DPI, the free radical scavenger NAC and the p38 MAPK inhibitor SB203580 blocked Hcy-induced VSMCs migration. Furthermore, atorvastatin suppressed Hcy-induced activation of NADPH oxidase and ROS, attenuated Hcy-induced overexpression of Nox1mRNA. Similar effects occurred with VSMCs transfected with Nox1 siRNA. Moreover, atorvastatin other than DPI, NAC, SB203580 and Nox1 siRNA transfection blocked Hcy-induced p38 MAPK phosphorylation, which was also reversed by the mevalonate. The data demonstrates that atorvastatin inhibits Hcy-induced VSMCs migration in a mevalonate pathway. Furthermore, a part of the biological effect of atorvastatin involves a decrease in the levels of Nox1-dependent ROS generation and p38 MAPK activation. © 2015 Wiley Publishing Asia Pty Ltd.
Li J.-P.,Xuhui District Central Hospital |
Zhang H.,Xuhui District Central Hospital |
He P.-D.,Xuhui District Central Hospital
Journal of Acupuncture and Tuina Science | Year: 2013
Objective: To observe the clinical efficacy of auricular point sticking combined with cupping in treating insomnia. Methods: Sixty-four patients with chronic insomnia were randomly divided into two groups. Thirty-two patients in the treatment group were treated with auricular point sticking combined with cupping therapy; while thirty-two patients in the control group were treated by Diazepam. The Pittsburgh sleep quality index (PSQI) were observed before and after treatment, and the data were statistically analyzed to evaluate the clinical effect. Results: After treatment, PSQI scores in both groups after treatment significantly decreased (P<0.05), and there was a significant difference between the two groups (P<0.05). In the improvement of daytime function, the treatment group was more efficient than the control group (P<0.05). The total effective rate of the treatment group was higher than that of the control group (P<0.05). Conclusion: Auricular point sticking combined with cupping therapy for insomnia is more effective than oral Diazepam, and it has a better effect in improving the patient's daytime function. © 2013 Shanghai Research Institute of Acupuncture and Meridian and Springer-Verlag Berlin Heidelberg.