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Qi Y.,University of Warwick | Qi Y.,Shanghai Xuhui Central Hospital | Thompson C.J.,Bruker | Van Orden S.L.,Bruker | O'Connor P.B.,University of Warwick
Journal of the American Society for Mass Spectrometry | Year: 2011

FT-ICR mass spectrometry has been limited to magnitudemode for almost 40 years due to the data processing methods used. However, it is well known that phase correction of the data can theoretically produce an absorption-mode spectrum with a mass-resolving power that is as much as twice as high as conventional magnitude mode, and that it also improves the quality of the peak shape. Temporally dispersed frequency sweep excitation followed by a time delay before detection results in a steep quadratic variation in the signal phase with frequency. Viewing this, it is possible to find the correct phase function by performing a quadratic least squares fit, modified by iterating through phase cycles until the correct quadratic function is found. Here, we present a robust manual method to rotate these signals mathematically and generate a "phased" absorption-mode spectrum. The method can, in principle, be automated. Baseline correction is also included to eliminate the accompanying baseline drift. The resulting experimental FT-ICR absorption-mode spectra exhibit a resolving power that is at least 50% higher than that of the magnitude mode. © American Society for Mass Spectrometry, 2011.


Dai T.,Peking Union Medical College | Dai T.,Shanghai Xuhui Central Hospital | Li K.,Shanghai Skin Disease Hospital | Lu H.,Shanghai Skin Disease Hospital | And 3 more authors.
Journal of Clinical Microbiology | Year: 2012

Previously, a small study showed that 14f was the predominant subtype of Treponema pallidum in Shanghai, China. The result was quite different from the genotype distribution in other areas of China. This study aimed to identify the strain types of Treponema pallidum in samples collected over a 5-year period in Shanghai. From 2007 to 2011, genital swabs were collected from patients with syphilis from the Shanghai Skin Disease Hospital. Positive specimens were typed by the enhanced typing method by adding a tp0548 gene to the existing arp and tpr genotype system. In total, 304 of the 372 enrolled patients yielded fully typeable DNA. Ten arp types (4, 6, 8, 9, 11, 12, 13, 14, 15, and 19), 3 tpr types (a, d, and o), and 5 tp0548 types (a, c, f, g, and i) were identified. In total, 12 subtypes were identified with a combination of the arp and tpr genes. Subtype 14d was found in 270 samples (88.8%). When the combination included the tp0548 gene, the 12 CDC subtypes identified were divided into 14 strain types. The predominant type was 14d/f (88.8%), followed by 15d/f (3.6%), 13d/f (1.3%), and 19d/c (1.3%). Two of the 44 14d/f-infected patients and both of the 19d/c-infected patients who underwent a lumbar puncture were diagnosed with neurosyphilis. This study showed that the predominant type in Shanghai was 14d/f. While this is in keeping with data from other areas in China, it is different from an earlier report showing that 14f is the most common genotype in Shanghai. Further studies are needed to better understand the association between strain types and neurosyphilis. Copyright © 2012, American Society for Microbiology. All Rights Reserved.


Jia J.-Y.,Shanghai Xuhui Central Hospital | Zhao Q.-H.,Fudan University | Liu Y.,Shanghai Xuhui Central Hospital | Gui Y.-Z.,CAS Shanghai Institute of Materia Medica | And 4 more authors.
Acta Pharmacologica Sinica | Year: 2013

Aim:Huperzine A isolated from the Chinese herb Huperzia serrata (Thunb) Trev is a novel reversible and selective AChE inhibitor. The aim of this study was to evaluate the pharmacokinetics and tolerance of single and multiple doses of ZT-1, a novel analogue of huperzine A, in healthy Chinese subjects.Methods:This was a double-blinded, placebo-controlled, randomized, single- and multiple-dose study. For the single-dose study, 9 subjects were randomly divided into 3 groups receiving ZT-1 (0.5, 0.75 or 1 mg, po) according to a Three-way Latin Square Design. For the multiple-dose study, 9 subjects receiving ZT-1 (0.75 mg/d, po) for 8 consecutive days. In the tolerance study, 40 subjects were randomly divided into 5 groups receiving a single dose of ZT-1 (0.5, 0.75, 1, 1.25 or 1.5 mg, po). Plasma and urine concentrations of ZT-1 and Hup A were determined using LC-MS/MS. Pharmacokinetic parameters, including C max, AUC 0-72 h and AUC 0-∞ were calculated. Tolerance assessments were conducted throughout the study.Results:ZT-1 was rapidly absorbed and converted into huperzine A, thus the plasma and urine concentrations of ZT-1 were below the limit of quantification (<0.05 ng/mL). After single-dose administration of ZT-1, the mean t max of huperzine A was 0.76-0.82 h; the AUC 0-72 h and C max of huperzine A showed approximately dose-proportional increase over the dose range of 0.5-1 mg. After the multiple-dose administration of ZT-1, a steady-state level of huperzine A was achieved within 2 d. No serious adverse events were observed.Conclusion:ZT-1 is a pro-drug that is rapidly absorbed and converted into huperzine A, and ZT-1 is well tolerated in healthy Chinese volunteers. © 2013 CPS and SIMM. All rights reserved.


Jin F.,Shanghai Xuhui Central Hospital | Xiong W.-J.,Shanghai Xuhui Central Hospital | Jing J.-C.,Shanghai Xuhui Central Hospital | Feng Z.,Shanghai Xuhui Central Hospital | And 2 more authors.
Journal of Cancer Research and Clinical Oncology | Year: 2011

Purpose: For decades of years, hundreds of candidate gene-based association studies explored the relationship between single nucleotide polymorphisms (SNPs) and hepatocellular carcinoma (HCC). There was no systematic review summarized the results of these association studies of candidate SNPs and HCC to date. In order to summarize the results of the association studies, we conducted a concise systematic review. Methods: By searching Pubmed database before October 2010, we reviewed all the association studies about candidate SNPs and HCC. If the eligible study number on a given SNP was more than three, we conducted a meta-analysis. We reported here only the overall positive-association results with statistical significance and evaluated the reliability of the associations by using false-positive report probability (FPRP) analysis and the Venice guidelines on genetic epidemiology studies. Results: Six SNPs of five genes (rs1800562 of HFE, rs17868323 and rs11692021 of UGT1A7, rs2279744 of MDM2, rs1143627 of IL-1B, and rs4880 of MnSOD) showed overall significant associations with HCC. The eligible number of the studies varied from three to nine. Two SNPs (rs1800562 of HFE and rs2279744 of MDM2) passed the FPRP threshold (FPRP < 0.20). According to the Venice guidelines, the associations between the two SNPs (rs1800562 and rs2279744) and HCC were of moderate evidence. Conclusions: Two SNPs (rs1800562 of HFE and rs2279744 of MDM2) were associated with HCC with moderate epidemiological evidence and deserve further study and additional biological and clinical assessment. © 2011 Springer-Verlag.


Qu L.-S.,Nantong University | Liu J.-X.,Nantong University | Kuai X.-L.,Nantong University | Xu Z.-F.,Nantong University | And 2 more authors.
Hepatology Research | Year: 2014

Aim: The impact of viral status on recurrence of hepatitis B-related hepatocellular carcinoma (HCC) after curative therapy remains controversial. This meta-analysis aimed to determine whether the presence of viral load, genotype, specific mutation and antiviral therapy influenced HCC recurrence after curative therapy. Methods: We performed a meta-analysis including 20 studies to assess the effect of viral status and antiviral therapy with nucleoside analog on recurrence of HCC after curative therapy. The pooled odds ratios (OR) were calculated using a random or fixed effects model. PUBMED, MEDLINE, EMBASE and the Cochrane Database were searched for articles published from 1990 to December 2012. Results: Our results showed that the presence of high viral load significantly increased overall HCC recurrence risk after curative therapy. Pooled data from four studies on the recurrence rate among patients with genotype C infection compared with genotype B showed an increased risk of recurrence. Basal core promoter (BCP) mutation was associated with a significant risk in the recurrence of HCC. The pooled estimate of treatment effect was significantly in favor of a preventive effectiveness of antiviral therapy. Conclusion: The present study suggested that HCC patients with high viral load, genotype C and BCP mutation had a significantly higher risk of recurrence. Antiviral therapy has potential beneficial effects after the curative treatment of HCC in terms of tumor recurrence. © 2013 The Japan Society of Hepatology.


Song Y.,Shanghai Xuhui Central Hospital | Ge W.,Shanghai Xuhui Central Hospital | Cai H.,Shanghai Xuhui Central Hospital | Zhang H.,Shanghai Xuhui Central Hospital
Journal of Cardiovascular Pharmacology and Therapeutics | Year: 2013

Viral myocarditis is an inflammation of the myocardium, and coxsackievirus B3 (CVB3) is one of the most important etiologic agents. Curcumin is an active ingredient of Curcumin longa, which has been used as a traditional Chinese herb for the treatment of various inflammatory diseases. The aim of this study was to explore the therapeutic effect of curcumin on CVB3-induced myocarditis and the underlying mechanism. Our results showed that treatment with curcumin could significantly attenuate CVB3-induced myocarditis, as demonstrated by improved weight loss, increased survival rate, reduced serological level cardiac enzymes, and improved heart histopathology. Of importance, curcumin administration was revealed to significantly reduce the systemic and local myocardial expression of proinflammatory cytokines, such as tumor necrosis factor-α, interleukin (IL) 6, and IL-1β, in the CVB3-infected mice. Further study showed that curcumin treatment significantly inhibited the CVB3-induced activation of nuclear factor-κB (NF-κB), a key transcription factor in the pathogenesis of inflammation, in a phosphatidylinositol 3 kinase (PI3K)/Akt pathway-dependent manner. These data indicate that curcumin has protective effect against CVB3-induced myocarditis by inhibiting PI3K/Akt/NF-κB signaling pathway and thus reducing the inflammatory response. © 2013 The Author(s).


Zhang Y.W.,Shanghai Xuhui Central Hospital
Orthopaedic surgery | Year: 2012

Using 64-slice spiral CT to reconstruct virtual knee joints of adults, we measured the gradient of proximal tibia-fibular joint and compared this result with bare joint measurement. We then discuss the accuracy of these measurements using a 64-slice spiral CT. Two hundred healthy adults were selected, including 100 males and 100 females, aged from 18 to 90 years. The gradient of the longitudinal axis of the fibula and the articular surface, and the angle of horizon of the proximal tibia-fibular articular surface and the horizontal line were measured by using a 64-slice spiral CT scan. The angle between the longitudinal axis of the fibula and the articular surface (angle A) was 56.80° ± 6.59°, both the left and the right fibula showed no significant difference (t = 0.308, P = 0.758). The angle between horizontal line and the articular surface (angle B) was 32.80° ± 6.49°. The regression or correlation analysis findings showed that there is a negative relationship (r = -0.952, P = 0.000), and there is significant difference (t = 51.87, P < 0.01) between the angle A and the angle B. The measurement value of using 64-slice spiral CT to reconstruct the slope of proximal tibia-fibular joints has no difference compared to the measurement value of the slope of bare proximal tibia-fibular joints. It is a precise, convenient method and is easy to apply for clinical study. © 2012 Tianjin Hospital and Blackwell Publishing Asia Pty Ltd.


Zhang H.,Shanghai Xuhui Central Hospital | Song Y.,Shanghai Xuhui Central Hospital | Zhang Z.,Shanghai Xuhui Central Hospital
American Journal of the Medical Sciences | Year: 2012

In this study, the authors explored the therapeutic effect of glycyrrhizin (GL) on Coxsackievirus B3 (CVB3)-induced myocarditis and its possible mechanisms involved. The results of this study showed that GL exhibited a profound amelioration of CVB3-induced myocarditis, as evidenced by improved weight loss profile, less increased serological levels of cardiac enzymes, less myocardial inflammation and increased survival rate. Further study showed that this effect was not due to the viral clearance but ascribed to weakened proinflamma-tory responses, as evidenced by significantly reduced expression of proinflammatory cytokines, including tumor necrosis factor-α, interleukin-1β and interleukin-6. Moreover, the authors demonstrated that GL administration inhibited CVB3-induced nuclear factor-κB activity efficiently by blocking the degradation of nuclear factor-κB inhibitor IκBκ. These data suggest that GL can effectively attenuate the severity of CVB3-induced myocarditis and may present as a new therapeutic approach for the treatment of viral myocarditis. © 2012 Southern Society for Clinical Investigation.


Rogge M.C.,Biogen Idec | Liu Y.,Shanghai Xuhui Central Hospital | Galluppi G.R.,Biogen Idec
Journal of Clinical Pharmacology | Year: 2014

Interferon beta-1a (IFNβ-1a) is a first-line therapy for relapsing multiple sclerosis when administered as 30 mcg intramuscularly (IM) once weekly. This endogenous cytokine displays pharmacokinetic (PK) attributes consistent with a glycoprotein of 20-kDa molecular weight that is administered IM. In this study, 24 healthy Chinese subjects (11 male, 13 female) each received 4 once-weekly 60-mcg IM doses of IFNβ-1a. Serial blood samples were drawn for PK and pharmacodynamic (PD) assessments following the first and last dose of drug. Results were compared with historical data from a recent PK/PD assessment conducted in non-Chinese subjects. Noncompartmental analysis revealed that no meaningful differences in either IFNβ-1a exposure or response were apparent between the Chinese and non-Chinese populations. Thus, it was concluded that no adjustment in dose regimen is warranted for future assessments of safety and efficacy in multiple sclerosis patients of Chinese origin. © 2014, The American College of Clinical Pharmacology.


Yang X.,Shanghai Xuhui Central Hospital
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2011

To improve the cognition of fungal ball sinusitis (FB) for diagnosing and treatting it rightly, we retrospectively analyzed the hospitalization cases. We analyzed 98 cases FB patients' first symptom, acoustic rhinometry, CT manifestation, operation fashion. The first symptoms of FB include: 47.2% nosebleed, 38.8% snuffle, 28.6% headache and face-ache, 4.0% nasal peculiar smell, 2.0% excessive tear, 4.0% no symptom. Anterior nasoscope inspection include: 43.9% no significant abnormity, 13.3% nasoseptal deviation. 41.8% nasal cavity neoplasm (nasal polyp, nasoturbinal polyp, nasal meatus fugal ball etc), 25.5% secretion in nasal cavity. The parameters that conclude the nasal airway resistance (NAR), nasal cavity volume (NCV) and nasal minimal cross-section area (NMCA) have extremely difference (P<0.01) between FB and normal volunteers (NV): the NAR increase, but NCV and NMCA decrease; the Distance of the Minimal cross-sectional area from the nostril (DCAN) appear moving backword compared with NV. The CT manifestation: almost unilateral lesions, involved paranasal sinuses present asymmetric or symmetrical opacification: 60.2% with calcified area, 25.5% with local bone erosion, 74.5% with sinus wall sclerosis. 65.3% of the CT diagnoses as FB are accord with pathology diagnoses. The incidence of FB involve maxillary sinus most (54.1%), then sphenoid sinus (11.2%) and ethmoid sinus (5.1%), frontal sinus (2.0%), multi-sinus (32.7%). All cases operated functional endoscopy surgery: 13 cases with nasoseptal diorthosis, 4 cases with inferior meatus opening, 2 case with canine fossa opening; we clean up the fungal pathogen with different degree endoscope through different path. The clinical symptom of FB is not representative; the acoustic rhinometry show that NAR increasing but NCV and NMCA decreasing maybe the reason of fungal infection; the CT examine is helpful for FB diagnosis; the functional endoscopy surgery is efficient therapy for FB.

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