Xuchang Institute of Forensic science

Henan’an, China

Xuchang Institute of Forensic science

Henan’an, China

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Bai R.,China University of Political Science and Law | Liu Y.,Xuchang Institute of Forensic science | Zhang J.,Xuchang Institute of Forensic science | Shi M.,China University of Political Science and Law | And 3 more authors.
International Journal of Legal Medicine | Year: 2016

A total of 1225 unrelated Han males from Henan province were analyzed with the prototype Yfiler® Plus kit (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA). The calculated gene diversity (GD) values ranged from 0.3855 to 0.9673 for the DYS391 and DYS385a/b loci, respectively. The discriminatory capacity (DC) was 86.94 % with 1065 observed haplotypes using 17 Yfiler loci, by the addition of 10 Y-STRs to the Yfiler® Plus system, the DC was increased to 98.94 % while showing 1212 observed haplotypes. Among the new incorporated Y-STRs, DYS576, DYF387S1, DYS518, DYS627, and DYS449 were major contributors to enhancing discrimination. In the analysis of molecular variance, the Henan Han population clustered with Asian origin populations and showed significant differences from other reference populations. In this study, the improvement of adding additional Y-STR markers with the Yfiler® Plus kit provided substantially stronger discriminatory power in the Henan Han population. © 2016 Springer-Verlag Berlin Heidelberg


Shi M.,Shantou University | Shi M.,China University of Political Science and Law | Liu Y.,Xuchang Institute of Forensic science | Zhang J.,Xuchang Institute of Forensic science | And 3 more authors.
Forensic Science International: Genetics | Year: 2015

We analyzed haplotypes for 24 Y chromosomal STRs (Y-STRs), including 17 Yfiler loci (DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DY438, DYS439, DYS448, DYS456, DYS458, DYS635 and Y-GATA-H4) and 7 additional STRs (DYS388, DYS444, DYS447, DYS449, DYS522 and DYS527a/b) in 1100 unrelated Chinese Han individuals from Henan Province using AGCU Y24 STR kit systems. The calculated average gene diversity (GD) values ranged from 0.4105 to 0.9647 for the DYS388 and DYS385a/b loci, respectively. The discriminatory capacity (DC) was 72.91% with 802 observed haplotypes using 17 Yfiler loci, by the addition of 7 Y-STRs to the Yfiler system, the DC was increased to 79.09% while showing 870 observed haplotypes. Among the additional 7 Y-STRs, DYS449, DYS527a/b, DYS444 and DYS522 were major contributors to enhancing discrimination. In the analysis of molecular variance, the Henan Han population clustered with Han origin populations and showed significant differences from other Non-Han populations. In the present study, we report 24 Y-STR population data in Henan Han population, and we emphasize the need for adding additional markers to the commonly used 17 Yfiler loci to achieve more improved discriminatory capacity in a population with low genetic diversity. © 2015 Published by Elsevier Ireland Ltd.


Bai R.,China University of Political Science and Law | Liu Y.,Xuchang Institute of Forensic science | Lizhe J.,China University of Political Science and Law | Lyv X.,China University of Political Science and Law | Shi M.,China University of Political Science and Law
Chinese Journal of Medical Genetics | Year: 2015

Objective: To analyze the genetic data of 30 insertion and deletion polymorphisms (InDel) loci included in an Investigator® DIPplex diagnostic kit, and to evaluate the forensic application in ethnic Tibetan population from China. Methods: By detecting 226 unrelated individuals with the Investigator® DIPplex kit, allelic frequencies and population genetics parameters of the 30 InDels were statistically analyzed and compared with available data derived from other populations from various regions. Results: After the Bonferroni correction at a 95% significance level (P = 0. 0017), no significant departures from the Hardy-Weinberg equilibrium were observed except for the HLD114 locus. Linkage disequilibrium test showed no significant allelic association between all 30 loci after the Bonferroni' s correction. The average heterozygosity (Ho) of all loci was 0. 4125, the mean discrimination power (DP) was 0. 5618, the mean polymorphism information content (PIC) was 0. 3280, and the combined discrimination power (TDP) was 0.999 999 999 990. The combined power of exclusion of all loci was 0. 987 849 91 in trio cases and 0. 949 771 25 in duo cases. Genetic distance between Tibetan and Han from Beijing was minimum (0. 0068) in the 5 populations, while genetic distance between Tibetan and Uygur was maximal (0. 0215). Conclusion: Multiplex detection has revealed that these 30 InDel loci have a moderate distribution of genetic polymorphism among ethnic Tibetan group residing in Tibet, China.


Shi M.,Shantou University | Shi M.,China University of Political Science and Law | Liu Y.,Xuchang Institute of Forensic science | Bai R.,Shantou University | And 4 more authors.
International Journal of Legal Medicine | Year: 2014

In this study, we assessed 30 insertion–deletion polymorphisms (Indels) (Investigator DIPplex® kit) in four Chinese populations (n = 952) and evaluated their usefulness in forensic genetic applications. After the Bonferroni correction at a 95 % significance level (p = 0.0017), there were no deviations from the Hardy–Weinberg equilibrium observed except for the HLD114 locus in the Tibetan ethnic group studied. A high level of discrimination power was observed for the DIPplex® kit in four sample populations (CDP > 0.9999) and the combined random match probabilities (CMP) were in the range of 1.4766 × 10−11 to 5.2742 × 10−13. Four Indels have been selected for further analyses as possible ancestry informative single nucleotide polymorphisms. The study support that the Investigator DIPplex® kit provides a powerful supplement to standard short tandem repeat-based kits for individual identification and kinship analysis in the Chinese population. © 2014, Springer-Verlag Berlin Heidelberg.


PubMed | Xuchang Institute of Forensic science, China University of Political Science and Law, Shantou University and Huazhong University of Science and Technology
Type: Journal Article | Journal: International journal of legal medicine | Year: 2016

A total of 1225 unrelated Han males from Henan province were analyzed with the prototype Yfiler Plus kit (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA). The calculated gene diversity (GD) values ranged from 0.3855 to 0.9673 for the DYS391 and DYS385a/b loci, respectively. The discriminatory capacity (DC) was 86.94% with 1065 observed haplotypes using 17 Yfiler loci, by the addition of 10 Y-STRs to the Yfiler Plus system, the DC was increased to 98.94% while showing 1212 observed haplotypes. Among the new incorporated Y-STRs, DYS576, DYF387S1, DYS518, DYS627, and DYS449 were major contributors to enhancing discrimination. In the analysis of molecular variance, the Henan Han population clustered with Asian origin populations and showed significant differences from other reference populations. In this study, the improvement of adding additional Y-STR markers with the Yfiler Plus kit provided substantially stronger discriminatory power in the Henan Han population.


PubMed | Xuchang Institute of Forensic science, China University of Political Science and Law and Shantou University
Type: | Journal: Forensic science international. Genetics | Year: 2015

We analyzed haplotypes for 24 Y chromosomal STRs (Y-STRs), including 17 Yfiler loci (DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DY438, DYS439, DYS448, DYS456, DYS458, DYS635 and Y-GATA-H4) and 7 additional STRs (DYS388, DYS444, DYS447, DYS449, DYS522 and DYS527a/b) in 1100 unrelated Chinese Han individuals from Henan Province using AGCU Y24 STR kit systems. The calculated average gene diversity (GD) values ranged from 0.4105 to 0.9647 for the DYS388 and DYS385a/b loci, respectively. The discriminatory capacity (DC) was 72.91% with 802 observed haplotypes using 17 Yfiler loci, by the addition of 7 Y-STRs to the Yfiler system, the DC was increased to 79.09% while showing 870 observed haplotypes. Among the additional 7 Y-STRs, DYS449, DYS527a/b, DYS444 and DYS522 were major contributors to enhancing discrimination. In the analysis of molecular variance, the Henan Han population clustered with Han origin populations and showed significant differences from other Non-Han populations. In the present study, we report 24 Y-STR population data in Henan Han population, and we emphasize the need for adding additional markers to the commonly used 17 Yfiler loci to achieve more improved discriminatory capacity in a population with low genetic diversity.


Bai R.,China University of Political Science and Law | Liu Y.,Xuchang Institute of Forensic science | Lv X.,Xuchang Institute of Forensic science | Shi M.,Xuchang Institute of Forensic science | Ma S.,China University of Political Science and Law
Forensic Science International: Genetics | Year: 2016

To evaluate the utility of Yfiler haplotype for DNA testing in two ethnic populations of China, a sample of 413 unrelated individuals (152 Shes and 261 Manchus) was determined. In the She and Manchu populations, the haplotype diversity was 0.9990 and 0.9988, respectively, and the discrimination capacity was 0.9474 and 0.9080, respectively. Research results will be valuable for human identification and paternity tests in the two minority regions and for Chinese population genetic study in the future. © 2016 Elsevier Ireland Ltd.


PubMed | Xuchang Institute of Forensic science and China University of Political Science and Law
Type: | Journal: Forensic science international. Genetics | Year: 2016

To evaluate the utility of Yfiler haplotype for DNA testing in two ethnic populations of China, a sample of 413 unrelated individuals (152 Shes and 261 Manchus) was determined. In the She and Manchu populations, the haplotype diversity was 0.9990 and 0.9988, respectively, and the discrimination capacity was 0.9474 and 0.9080, respectively. Research results will be valuable for human identification and paternity tests in the two minority regions and for Chinese population genetic study in the future.

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