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Buahom N.,China Agricultural University | Du Y.,Yunnan Entry Exit Inspection and Quarantine Bureau | Wu Y.,Chinese Academy of Sciences | Deng Y.,Xishuangbanna Entry Exit Inspection and Quarantine Bureau | And 3 more authors.
Applied Entomology and Zoology | Year: 2013

Bactrocera correcta (Bezzi) is one of the most destructive insect pests of fruits and vegetables in tropical and subtropical regions. At present, this fly is primarily distributed in Southeast Asia. Twelve microsatellite loci were isolated from an enriched genomic library based on a biotin/streptavidin capture protocol. The polymorphism of these loci was tested on 74 individual flies from two natural populations. Allele number ranged from 6 to 14 and 10 loci demonstrated a polymorphic information content (PIC) greater than 0.5. The pairwise F ST value between the two populations was 0.0048 (P > 0.05). These microsatellite loci have potential utility for studies of population genetic structure in this species. © 2013 The Japanese Society of Applied Entomology and Zoology.

Jiang F.,China Agricultural University | Li Z.H.,China Agricultural University | Deng Y.L.,Xishuangbanna Entry Exit Inspection and Quarantine Bureau | Wu J.J.,Inspection and Quarantine Technology Center | And 2 more authors.
Bulletin of Entomological Research | Year: 2013

Abstract The guava fruit fly, Bactrocera correcta (Bezzi) (Diptera: Tephritidae), is an invasive pest of fruit and vegetable crops that primarily inhabits Southeast Asia and which has the potential to become a major threat within both the Oriental and Australian oceanic regions as well as California and Florida. In light of the threat posed, it is important to develop a rapid, accurate and reliable method to identify B. correcta in quarantine work in order to provide an early warning to prevent its widespread invasion. In the present study, we describe a species-specific polymerase chain reaction assay for the diagnosis of B. correcta using mitochondrial DNA cytochrome oxidase I (mtDNA COI) barcoding genes. A B. correcta-specific primer pair was designed according to variations in the mtDNA COI barcode sequences among 14 fruit fly species. The specificity and sensitivity of the B. correcta-specific primer pair was tested based on the presence or absence of a band in the gel profile. A pair of species-specific B. correcta primers was successfully designed and named BCOR-F/BCOR-R. An ∼280 bp fragment was amplified from specimens belonging to 17 geographical populations and four life stages of B. correcta, while no such diagnostic bands were present in any of the 14 other related fruit fly species examined. Sensitivity test results demonstrated that successful amplification can be obtained with as little as 1 ng μl -1 of template DNA. The species-specific PCR analysis was able to successfully diagnose B. correcta, even in immature life stages, and from adult body parts. This method proved to be a robust single-step molecular technique for the diagnosis of B. correcta with respect to potential plant quarantine. © Cambridge University Press 2013.

Ma X.,China Agricultural University | Li Z.,China Agricultural University | Wu J.,Guangdong Entry Exit Inspection and Quarantine Bureau | Ma J.,Guangdong Entry Exit Inspection and Quarantine Bureau | And 2 more authors.
Sensor Letters | Year: 2012

The guava fruit fly, Bactrocera correcta (Bezzi) (Diptera: Tephritidae), originates from the tropical and subtropical regions of Asia and affects a large variety of fruits. It is regarded as a dangerous fruit fly and is regulated by quarantine measures in China. This study assessed the introduction risk of the guava fruit fly associated with the importation of host fruit into China. The risk assessment based on historical data, expert opinions, relevant literature and archives was used to determine appropriate parameters in the pathway analysis. With a computational model, Monte Carlo simulations were conducted using Decision Tools Suite 5.5 Industrial Edition to estimate the probability of the introduction of the guava fruit fly. Risk management options were incorporated and risk analysis was performed to determine how the risk could be reduced. The study indicated the probability of introduction into China of the guava fruit fly via imported host fruit with current quarantine measures is very low at 1.06E-12. In contrast, the probability is high at 0.1049 without entry detection. Sensitivity analysis was performed to assess the model stability and the impact of parameter changes. Based on the sensitivity analysis, the most critical input was entry detection, followed by mitigation treatment and the number of guava fruit flies per ton of infested host fruit, respectively. We concluded that intensive detection in conjunction with maintaining mitigation treatment would significantly reduce the risk of introduction. © 2012 American Scientific Publishers. All rights reserved.

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