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Jing S.,Xinyang Normal University | Zheng B.,Xinyang Normal University | Qiao L.,Xinyang City Academy of Agricultural science | Zhang K.,Xinyang Normal University | And 6 more authors.
Applied Entomology and Zoology | Year: 2016

The tea geometrid, Ectropis obliqua Prout (Lepidoptera: Geometridae), is one of the most threatening pests of tea (Camellia sinensis (L.) O. Kuntze) plants. In this study, we developed eleven new polymorphic microsatellite markers from tea geometrid by using the microsatellite motif enriched library method. Polymorphism of each locus was detected in 69 individuals from three natural populations. The number of alleles varied from 2 to 8, and the expected and observed heterozygosities ranged from 0.042 to 0.801 and from 0.042 to 0.875, respectively. Six loci had significant deviations from the Hardy–Weinberg equilibrium (HWE). These microsatellite markers will be useful for the future study of this agricultural pest in genetic diversity, population structure and evolution of resistance. This information will be allowed to establish new pest management strategies and improve the effectiveness of pest control program of this species. © 2016, The Japanese Society of Applied Entomology and Zoology.


Ren F.-J.,Northwest University, China | Xie Q.,University of Art | Qiao L.,Northwest University, China | Qiao L.,Xinyang City Academy of Agricultural science | Qin D.-Z.,Northwest University, China
ZooKeys | Year: 2014

One new species of the delphacid genus Kakuna Matsumura, K. taibaiensis Ren & Qin, sp. n. is described from Mt. Taibai in Shaanxi Province, China. Dicranotropis montana (Horvath, 1897) is reported for the first time from China. Habitus photos and illustrations of male genitalia of the two species are given. © Feng-Juan Ren et al.


Qiao L.,Northwest University, China | Qiao L.,Xinyang City Academy of Agricultural science | Wu J.X.,Northwest University, China | Qin D.Z.,Northwest University, China | And 6 more authors.
Journal of Insect Science | Year: 2015

Empoasca onukii Matsuda is a worldwide pest that causes great economic loss in tea growing areas and is significantly affected by temperatures. Heat shock protein (Hsp) genes are important in insects' response to temperature stress. In this study, two full-length Hsp genes, Eohsp90 and Eohsp70, were cloned from E. onukii using rapid amplification of complementary DNA ends. The open reading frames of Eohsp90 and Eohsp70 were 2,172 bp and 2,016 bp in length, respectively. Their deduced amino acid sequences of Eohsp90 and Eohsp70 showed high homology with other species. Subsequently, the transcriptional expression of Eohsp90 and Eohsp70 in E. onukii adults exposed to various temperatures (-5, 0, 10, 15, 20, 25, 30, 35, 38, 41 and 44°C) for 1 h, and at extreme temperatures (0°C and 41°C) for various time duration (0, 20, 40, 60, 80, 100, and 120 min) were investigated via real-time quantitative polymerase chain reaction. The relative expression levels of both Eohsp90 and Eohsp70 in E. onukii adults were upregulated as the temperature rises or falls over time, except in the -5°C or 44°C temperature groups. Moreover, the expression level in the temperature elevated groups was higher than that of the lower temperature groups. In addition, the Eohsp70 generally demonstrated a higher transcriptional level than Eohsp90, and both genes had a higher expression profile in female adults compared with the males. The expression profiles indicated that Eohsp90 and Eohsp70 may play important roles in E. onukii adult responses to ecologically relevant environmental temperature threat. © Crown copyright 2015.


PubMed | Northwest Agriculture and Forestry University and Xinyang City Academy of Agricultural science
Type: | Journal: Journal of insect science (Online) | Year: 2015

Empoasca onukii Matsuda is a worldwide pest that causes great economic loss in tea growing areas and is significantly affected by temperatures. Heat shock protein (Hsp) genes are important in insects response to temperature stress. In this study, two full-length Hsp genes, Eohsp90 and Eohsp70, were cloned from E. onukii using rapid amplification of complementary DNA ends. The open reading frames of Eohsp90 and Eohsp70 were 2,172bp and 2,016bp in length, respectively. Their deduced amino acid sequences of Eohsp90 and Eohsp70 showed high homology with other species. Subsequently, the transcriptional expression of Eohsp90 and Eohsp70 in E. onukii adults exposed to various temperatures (-5, 0, 10, 15, 20, 25, 30, 35, 38, 41 and 44C) for 1h, and at extreme temperatures (0C and 41C) for various time duration (0, 20, 40, 60, 80, 100, and 120min) were investigated via real-time quantitative polymerase chain reaction. The relative expression levels of both Eohsp90 and Eohsp70 in E. onukii adults were upregulated as the temperature rises or falls over time, except in the -5C or 44C temperature groups. Moreover, the expression level in the temperature elevated groups was higher than that of the lower temperature groups. In addition, the Eohsp70 generally demonstrated a higher transcriptional level than Eohsp90, and both genes had a higher expression profile in female adults compared with the males. The expression profiles indicated that Eohsp90 and Eohsp70 may play important roles in E. onukii adult responses to ecologically relevant environmental temperature threat.

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