Xi J.,Xinxiang Medical College
Wei sheng yan jiu = Journal of hygiene research | Year: 2010
OBJECTIVE: To study the heavy metal contamination status in river water and mud in the suburb of a city in Henan Province. METHODS: Typical sampling method is used to select a farmland irrigation river of the suburb of a city. Use the atomic absorption spectrophotometry, and measure the heavy metal cadmium (Cd), copper (Cu), lead (Pb) in the river water samples and mud samples by graphite furnace method and flame method, respectively. The results of water were compared with GB 3838-2002, Environmental Quality Standards for Surface Water, and GB 5084-2005, Standards for Irrigation Water Quality. The results of mud were compared with national soil background value. The contents of Cu and Cd in the river samples do not exceed the standard, and that of Pb is 3 to 6 times higher than the standard. According to the single factor pollution index method, the single factor pollution indice of Cu, and Cd in the river are less than 0.2 and are of clean level, while that of Pb reaches 6.84, indicating the Pb pollution in river water is severe. Cu in mud is more than 4 times of the soil background value, and that of Cd is more than 69 times of the soil background value, and that of Pb is more than 2 times of the soil background value. The single item pollution index indicates, in mud, the pollution index of Pb is 2.5, medium level pollution. The pollution indice of Cu and Cd in mud are more than 3, is severe pollution, and the Cd pollution is especially heavy, and the single pollution index reaches 67.76. The comprehensive pollution indice of the river and the mud are 5.346 and 84.115, respectively, indicating that both are at heavy pollution level. The main pollution source of the river originates from Pb, and that of the mud is from Cd and it is required to take measure and control as early as possible.
Xu C.Y.,Xinxiang Medical College
Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology | Year: 2010
To study the effect of progesterone on matrix metalloproteinase-3 (MMP-3) expression in neonatal rat brain after hypoxic-ischemia. Followed the hypoxic-ischemia of neonatal rat brain, Evans blue (EB) staining and transmission electron microscopy were used to detect the blood-brain barrier pathological changes on permeability. MMP-3 protein expression in cerebral cortex was measured with Western blot. Transmission electron microscopy results showed that the blood brain barrier in hypoxic-ischemic group changed significantly compare to progesterone group. EB staining results suggested that the blood-brain barrier permeability of hypoxic-ischemic group was significantly increased compared to sham-operated group (P < 0.01). The blood-brain barrier permeability in progesterone group was also decreased in comparison to that of hypoxic-ischemic group (P < 0.05). Western blot image analysis results indicated that MMP-3 protein expression in the hypoxic-ischemic group increased significantly than that in sham-operated group (P < 0.01), and the progesterone group was decreased significantly than that in hypoxic-ischemic group (P < 0.05). Progesterone may reduce the blood-brain barrier damage by reducing MMP-3 expression. This might be one of the protective mechanisms in the hypoxic-ischemic brain injury.
Zhu F.,Xinxiang Medical University |
Liu P.,Xinxiang Medical University |
Li J.,Xinxiang Medical University |
Zhang Y.,Xinxiang Medical College
Oncology Reports | Year: 2014
Chemokines have been reported to play crucial roles in tumor progression. Eotaxin-1 (CCL11), a member of the CC chemokine family, is elevated in many types of human cancer. Here, to reveal the molecular mechanisms of eotaxin-1 in prostate cancer cell invasion, the expression of eotaxin-1 receptors [CC chemokine receptor (CCR)2, CCR3 and CCR5] were silenced by small interfering RNA (siRNA). The ERK pathway was inhibited by the specific MEK inhibitor U0126. The role of eotaxin-1 and the CCR3-ERK pathway in prostate cancer cell invasion was assessed by invasion and migration assays. MMP-3 expression was detected by real-time PCR and ELISA assay. The results demonstrated that eotaxin-1 promoted the invasion and migration of DU-145 cells, and increased ERK1/2 activation and MMP-3 expression. Knockdown of CCR3 inhibited the invasion and migration of prostate cancer cells, and attenuated the eotaxin-1-induced ERK1/2 activation and MMP-3 expression. Furthermore, inactivation of the ERK pathway suppressed the eotaxin.1-promoted invasion and migration, and decreased MMP-3 expression in the prostate cancer cells. Together, the present study suggests that eotaxin-1 increases MMP-3 expression via the CCR3-ERK pathway, thereby promoting prostate cancer cell invasion and migration. Thus, therapies that block eotaxin-1 and CCR3 may be effective interventions for prostate cancer.
Liu T.-G.,Henan University |
Liu T.-G.,Xinxiang Medical College |
Zhao G.-F.,Henan University |
Wang Y.-X.,Henan University
Physics Letters, Section A: General, Atomic and Solid State Physics | Year: 2011
The geometries, stabilities, electronic and magnetic properties of GdSin (n=1-17) clusters have been investigated systematically by using density functional theory (DFT) with the generalized gradient approximation (GGA). We find that the Gd atom in the lowest-energy configuration gradually moves from convex to surface, and to the interior site as the number of Si atom varies from 1 to 17. Furthermore, from GdSi16, Gd atom in GdSi16 cluster completely falls into the center of the Si framework and forms a fullerene cage. On the basis of the atomic average binding energy [Eb(n)], the second difference in energy (Δ2E) and VIP, we predict that the magic number of GdSin (n=1-17) clusters should be 5, 11 and 16, and these clusters should be abundant in the mass spectra when n=5, 11 and 16. The magnetic moments of the GdSin clusters are also studied. It is found that the total magnetic moments and the magnetic moments on Gd of GdSin clusters are large, and they do not quench when Gd is encapsulated in Si outer framework cage, which is to a large extent due to the fact that the Gd 4f electrons hardly interact with the silicon cage. © 2011 Elsevier B.V. All rights reserved.
Sun X.,Xinxiang Medical College
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2013
To study the gene expressions of suppressors of cytokine signaling (SOCS1, 2, 3) and cytokine-inducible SH2-domain 1(CIS-1) in chondrocytes of osteoarthritis (OA) patients and healthy controls, and also analyze the effects of IL-1β and TNF-α on the levels of mRNA encoding these SOCS family members. Chondrocytes were isolated from patients of OA and joint replacement due to traffic accident by sequential treatment with trypsin, hyaluronidase and collagenase B. Total RNA was extracted from the same chondrocytes, and the levels of SOCS1, 2, 3 and CIS-1 mRNA were determined by real-time qRT-PCR. In addition, healthy chondrocytes were cultured with and without a mixture of IL-1β and oncostatin M (OSM, both 2.5 ng/mL) or TNF-α (10 ng/mL). The short-term cultures with single cytokine treatment were harvested 24 and 72 h after treatment, and the long-term cultures were maintained for 4-5 weeks until confluent with periodical cytokine stimulation. Total RNA was extracted and mRNA levels of SOCS1, 2, 3 and CIS-1 mRNA were detected by qRT-PCR. The SOCS2 and CIS-1 mRNA levels were reduced by approximately 8-fold in OA samples compared to controls (P<0.01), whereas SOCS1 and SOCS3 showed similar expression patterns in OA and control chondrocytes (P>0.05). The SOCS2 and CIS-1 mRNA levels declined by 5-fold and 3-fold with long-term treatment with IL-1β and OSM or IL-1β and TNF-α, respectively (P<0.05). In OA patients, the expressions of SOCS2 and CIS-1 decreased, while SOCS1 and SOCS3 were unaffected. Long-term treatment with inflammatory cytokines mimicking OA effect attenuated the expressions of SOCS2 and CIS-1 in chondrocytes.