Xinqiao Hospital

Chongqing, China

Xinqiao Hospital

Chongqing, China
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Li F.,Jilin Agricultural Science and Technology College | Li F.,Yanshan University | Zhang Y.,Xinqiao Hospital | Zhong Z.,University of Troms
International Journal of Molecular Sciences | Year: 2011

The current study evaluated the glucoselowering effect of ganoderma lucidum polysaccharides (GlPS) in streptozotocin (STZ)induced diabetic mice. The diabetic mice were randomly divided into four groups (8 mice per group): diabetic control group, lowdose GlPS treated group (50 mg/kg, GlPS), highdose GlPS treated group (150 mg/kg, GlPS) and positive drug control treated group (glibenclamide, 4 mg/kg), with normal mice used as the control group. Body weights, fasting blood glucose (FBG), serum insulin and blood lipid levels of mice were measured. After 28 days of treatment with GlPS, body weights and serum insulin levels of the GlPS treated groups was significantly higher than that of the diabetic control group, whereas FBG levels was significantly lower. Moreover, total cholesterol (TC), triglyceride (TG) and low density lipoprotein cholesterol (LDLC) levels of the GlPS treated groups had dropped, whereas the high density lipoprotein cholesterol (HDLC) levels had increased. In addition, according to acute toxicity studies, GlPS did not cause behavioral changes and any death of mice. These data suggest that GlPS has an antihyperglycemic effect. Furthermore, considering the GlPS effects on lipid profile, it may be a potential hypolipidaemic agent, which will be a great advantage in treating diabetic conditions associated with atherosclerosis or hyperlipidemia. © 2011 by the authors; licensee MDPI, Basel, Switzerland.


Jian W.,Xinqiao Hospital
Pain Physician | Year: 2013

Background: Vertebral hemangioma (VH) is considered to be a benign lesion of bone with a rich vasculature. Most incidentally discovered hemangiomas are asymptomatic. Percutaneous vertebroplasty (PVP) has demonstrated efficacy in the treatment of symptomatic thoracic and lumbar VHs. To our knowledge, the reports concerning PVP on symptomatic cervical VHs are quite rare. Objective: Our intent was to assess PVP as treatment for symptomatic cervical hemangiomas. Study Design: Prospective evaluation. Setting: An inteventional pain management practice, single medical center. Methods: Eight patients with symptomatic cervical VHs were enrolled between December 2008 and February 2012, all of whom underwent magnetic resonance imaging (MRI) of the cervical spine. The patients with 8 vertebral bodies of VHs were treated by using PVP. The clinical and radiological data were collected and analyzed. Postoperative outcomes were determined using a visual analog scale. Results: Surgical levels include C3 (2 patients), C4 (3 patients), C5 (2 patients), and C6 (one patient). The average follow-up period was 27.4 months, with a minimum of 12 months. Mean operative time and x-ray exposure time were respectively 35 ± 7.1 minutes and 25 ± 7.7 seconds. The visual analogue scale for neck pain decreased significantly from 6.9 ± 0.6 preoperatively to 1.3 ± 0.5 (P < 0.01) on the second day after surgery, with a final score of 1.2 ± 0.5 (P < 0.01). Cement distribution was always diffuse and homogeneous. No cement leakage was observed in all cases except for 2 patients. There were no other major complications at the time of last follow-up evaluation. Limitations: An observational clinical trial with a relatively small sample size. Conclusion: Short-term results indicate that PVP appears to be an effective and safe treatment for symptomatic cervical VHs.


Lin S.,Xinqiao Hospital
Chinese Journal of Oncology | Year: 2011

Objective: To isolate and identify the cancer stem cells from primary human ovarian cancer tissues. Methods: Fresh tumor tissues from five cases of pathologically diagnosed ovarian cancers were taken, minced and then digested with collagenase and hyaluronidase to obtain single cell suspension. The erythrocytes were removed with ACK Lysis buffer. The suspensions were sorted by magnetic activated cell sorting (MACS) using CD133-binding microbeads. Then the sorted CD133+ cells were verified by flow cytometry. The cells were cultured in serum-free medium supplemented with EGF, bFGF, insulin and BSA, and grew into spheroids. Immunofluorescence, differentiation and tumor formation tests of the cells were performed to characterize the properties of cancer stem cells. Results: The ovarian cancer stem cells were successfully isolated from primary human ovarian tumors, which formed typical spheroids in serum-free medium and had stronger ability of tumorigenesis. The results of related experiments verified that CD133 positive cells owned the properties of cancer stem cells. Conclusions: The ovarian cancer stem cells presenting the characteristics of sternness in vitro and in vivo, have been successfully isolated from primary human ovarian tumor tissues by MACS. The isolated ovarian cancer stem cells could be used in future researches of their biological functions.


Wang J.,Chongqing Medical University | Zhao J.,Chongqing Medical University | Shi M.,Chongqing Medical University | Ding Y.,Xinqiao Hospital | And 3 more authors.
PLoS ONE | Year: 2014

Background: MiRNAs are important regulators of different biological processes, including tumorigenesis. MiR-210 is a potential prognostic factor for survival in patients with cancer according to previous clinical researches. We conducted a systematic review and meta-analysis to summarize the significance of increased miR-210 expression in the prognosis of indicated cancers. Methodology/Principal Findings: The present systematic review and meta-analysis of 16 researches included 1809 patients with 7 different types of cancers from 7 countries, and aimed to explore the association between miR-210 expression and the survival of cancer patients. Over-expression of miR-210 may predict poor overall survival (OS, HR = 1.33, 95% CI: 0.85-2.09, P = 0.210), but the effect was not significant. While the predictive effect on disease-free survival (DFS, HR = 1.89, 95% CI: 1.30-2.74, P = 0.001), progression-free survival (PFS, HR = 1.20, 95% CI: 1.05-1.38, P = 0.007) and relapse-free survival(RFS, HR = 4.42, 95% CI: 2.14-9.15, P = 0.000) for patients with breast cancer, primary head and neck squamous cell carcinoma (HNSCC), renal cancer, soft-tissue sarcoma, pediatric osteosarcoma, bladder cancer or glioblastoma was certain. Subgroup analysis showed the limited predictive effect of over-expressed miR-210 on breast cancer OS (HR = 1.63, 95% CI: 0.47-5.67, P = 0.443), breast cancer DFS (HR = 2.03, 95% CI: 0.90-4.57, P = 0.088), sarcoma OS (HR = 1.24, 95% CI: 0.20-7.89, P = 0.818) and renal cancer OS (HR = 1.16, 95% CI: 0.27-4.94, P = 0.842). Conclusions/Significance: This systematic review and meta-analysis suggests that miR-210 has a predictive effect on survival of patients with studied cancer types as indexed by disease-free survival, progression-free survival and relapse-free survival. While the predictive effect on overall survival, breast cancer overall survival, breast cancer disease-free survival, sarcoma overall survival and renal cancer overall survival was not statistically significant. © 2014 Wang et al.


Li X.,Purdue University | Zhang C.,Purdue University | Hao C.,Purdue University | Tian C.,Purdue University | And 2 more authors.
ACS Nano | Year: 2012

This paper reports a strategy for DNA self-assembly. Cross-over-based DNA nanomotifs are held together by T-junctions instead of commonly used sticky-end cohesion. We have demonstrated this strategy by assembling a DNA tetrahedron, an octahedron, and an icosahedron. The resulting DNA polyhedra contain out-pointing, short DNA hairpin spikes. These hairpins are well-structured relative to the polyhedra core and provide potential locations for introduction of functional chemicals such as proteins and gold nanoparticles. The T-linked DNA polyhedra have been characterized by polyacrylamide gel electrophoresis, atomic force microscopy, and dynamic light scattering. © 2012 American Chemical Society.


Qiu Y.,Xinqiao Hospital | Yang Y.,Xinqiao Hospital | Yang H.,Xinqiao Hospital
Digestive Diseases and Sciences | Year: 2014

Interspersed among epithelial cells (ECs), intraepithelial lymphocytes (IELs) might be important constituents of the physiological and immunological barriers of the intestinal epithelial layer. IELs are composed of memory-effector T cell subtypes bearing the T cell receptor-γδ (TCRγδ) and TCRαβ. The intimate cell adhesion molecules- and tight junction proteins-mediated biological interactions between IELs and ECs ensure that IELs can reside within the intraepithelial compartment and survey large areas of the villus epithelium. As sentinels in this critical interface, IELs express TCRs that recognize antigenic peptides presented by conventional major histocompatibility complex (MHC) molecules or by non-classical MHC molecules. Moreover, IELs monitor for stressed or damaged ECs to mediate pathological responses and maintain intestinal homeostasis. In this review, we address how IELs reside within the epithelium and exert their sentinel functions. © Springer Science+Business Media New York 2013.


Yu J.,Purdue University | Liu Z.,Purdue University | Jiang W.,Purdue University | Wang G.,Xinqiao Hospital | Mao C.,Purdue University
Nature communications | Year: 2015

Rational, de novo design of RNA nanostructures can potentially integrate a wide array of structural and functional diversities. Such nanostructures have great promises in biomedical applications. Despite impressive progress in this field, all RNA building blocks (or tiles) reported so far are not geometrically well defined. They are generally flexible and can only assemble into a mixture of complexes with different sizes. To achieve defined structures, multiple tiles with different sequences are needed. In this study, we design an RNA tile that can homo-oligomerize into a uniform RNA nanostructure. The designed RNA nanostructure is characterized by gel electrophoresis, atomic force microscopy and cryogenic electron microscopy imaging. We believe that development along this line would help RNA nanotechnology to reach the structural control that is currently associated with DNA nanotechnology.


Tian C.,Purdue University | Li X.,Purdue University | Liu Z.,Purdue University | Jiang W.,Purdue University | And 2 more authors.
Angewandte Chemie - International Edition | Year: 2014

Tile-based self-assembly is a powerful method in DNA nanotechnology and has produced a wide range of well-defined nanostructures. But the resulting structures are relatively simple. Increasing the structural complexity and the scope of the accessible structures is an outstanding challenge in molecular self-assembly. A strategy to partially address this problem by introducing flexibility into assembling DNA tiles and employing directing agents to control the self-assembly process is presented. To demonstrate this strategy, a range of DNA nanocages have been rationally designed and constructed. Many of them can not be assembled otherwise. All of the resulting structures have been thoroughly characterized by gel electrophoresis and cryogenic electron microscopy. This strategy greatly expands the scope of accessible DNA nanostructures and would facilitate technological applications such as nanoguest encapsulation, drug delivery, and nanoparticle organization. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Wang H.W.,Xinqiao Hospital
Orthopaedic surgery | Year: 2013

To investigate the characteristics of patients with traumatic vertebral fractures and concomitant fractures of the first rib and their management. From January 2001 to December 2010, 17/3142 patients (0.5%) with traumatic vertebral fractures who presented to our hospitals had concomitant fractures of the first rib. The study included 14 men (82.4%) and 3 women (17.6%) patients of age 32-57 years (mean, 46.6 years). The mechanisms of trauma were falls from heights in seven, motor vehicle accidents in five and direct collisions with blunt objects in five. Thirteen patients (76.5%) presented initially with pulmonary complications after sustaining trauma. Three patients sustained one rib fracture, two three rib fractures, three four rib fractures and 10 > five rib fractures. The injuries were right-sided in three cases, left-sided in three and bilateral in eleven. Four patients (23.5%) presented with craniocerebral injuries. According to the American Spinal Injury Association (ASIA) classification, 10 patients (58.8% of the total study group) had motor and sensory deficits (ASIA A-D). There were no vascular injuries or deaths. Traumatic vertebral fractures with concomitant fractures of the first rib are associated with multisystem injuries, but not always with morbidity and mortality. A multidisciplinary approach, early diagnosis, appropriate treatment and observation in the intensive care unit may prevent morbidity and/or mortality. © 2013 Chinese Orthopaedic Association and Wiley Publishing Asia Pty Ltd.


Hao C.,Purdue University | Li X.,Purdue University | Tian C.,Purdue University | Jiang W.,Purdue University | And 2 more authors.
Nature Communications | Year: 2014

RNA nanotechnology promises rational design of RNA nanostructures with wide array of structural diversities and functionalities. Such nanostructures could be used in applications such as small interfering RNA delivery and organization of in vivo chemical reactions. Though having impressive development in recent years, RNA nanotechnology is still quite limited and its programmability and complexity could not rival the degree of its closely related cousin: DNA nanotechnology. Novel strategies are needed for programmed RNA self-assembly. Here, we have assembled RNA nanocages by re-engineering a natural, biological RNA motif: the packaging RNA of phi29 bacteriophage. The resulting RNA nanostructures have been thoroughly characterized by gel electrophoresis, cryogenic electron microscopy imaging and dynamic light scattering. copyright © 2014 Macmillan Publishers Limited.

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