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Wang W.-P.,Central South University | Wang W.-P.,Xinjiang Uygur Autonomous Region Institute for Food and Drug Control | Lin J.,Xinjiang Uygur Autonomous Region Institute for Food and Drug Control | Zhang L.-X.,Chinese Academy of Agricultural Sciences | And 2 more authors.
Journal of Central South University | Year: 2013

A simple and facile gas chromatography-mass spectrometer (GC-MS) fingerprint of Su-He-Xiang-Wan (SHXW) was developed, the similarity analysis was conducted, and attribution of the major characteristic peaks was identified for SHXW quality control. GC-MS analysis was performed on a QP2010 instrument (Shimadzu, Japan) equipped with a capillary column of RTX-5MS. The column temperature was initiated at 50 C, held for 5 min, increased at the rate of 3 C/min to 120 C, held for 2 min, and then increased at the rate of 4 C/min to 220 C, held for 10 min. Helium carrier gas was used at a constant flow rate of 1.3 mL/min. Mass conditions were ionization voltage, 70 eV; injector temperature, 250 C; ion source temperature, 250 C; splitting ratio, 30:1; full scan mode in the 40-500 Da mass ranges with rate of 0.2 s per scan. Attribution of the major characteristic peaks was identified for SHXW by comparing the chemical standards, references of Chinese herbal medicines and the negative controls of prescription samples (NC) of SHXW. With the help of the temperature-programmed retention indices (PTRIs) used together with mass spectra and chemical standards, 25 major characteristic peaks have been identified. Nine volatile medicinal materials were identified in the prescription of SHXW by attributing to the 27 major characteristic peaks. The results demonstrate that the proposed method is a powerful approach to quality control of complex herbal medicines. © 2013 Central South University Press and Springer-Verlag Berlin Heidelberg.


Wang W.-P.,Central South University | Wang W.-P.,Xinjiang Uygur Autonomous Region Institute for Food and Drug Control | Zhang M.-Y.,Xinjiang Uygur Autonomous Region Institute for Food and Drug Control | Aziz M.,Xinjiang Uygur Autonomous Region Institute for Food and Drug Control | And 2 more authors.
Journal of Central South University | Year: 2013

A simple and sensitive high performance liquid chromatography-chemical vapour generation-atom fluorescent spectrometry (HPLC-CVG-AFS) method was developed and validated for simultaneous determination mercury species in Su-He-Xiang-Wan (SHXW) and in tissues of rats, respectively. The species of mercury were separated by a Venusil MP-C18 (5 μm, 150 mm×4.6 mm) column with the optimized mobile phase containing 5% (w/v) acetonitrile, 0.01 mol/L L-cysteine and 0.06 moL/L ammonium acetate. The tissues of rats were freeze-dried after giving the medicine for 10 d, and then added into the solution containing 10% (w/v) HCl, 1% (w/v) sulfocarbamide and 0.15% (w/v) KCl for increasing extraction rate. The resolutions of Hg2+, MeHg and EtHg were 1.5 and 2.9, respectively. The detection limits of Hg2+, MeHg and EtHg were 2.0, 1.0 and 0.9 ng/mL, respectively. The relative standard deviation (RSD) of inter- and intra-day precisions ranged from 1.56% to 2.86%. The recovery rates of three different adding level were 87%-101% (n=6), and the RSDs were smaller than 8.2%. The results show that no MeHg and EtHg were detected in rat tissues. Only soluble mercury (Hg2+) was determined for the mercury species of SHXW in rat tissues. © 2013 Central South University Press and Springer-Verlag Berlin Heidelberg.


Liang Y.-Z.,Central South University | Wang W.-P.,Central South University | Wang W.-P.,Xinjiang Uygur Autonomous Region Institute for Food and Drug Control
Chimia | Year: 2011

The holistic system of traditional Chinese medicine (TCM) is reflected by the integrity of the ingredients contained in herbal medicines, which creates a challenge in establishing quality control standards for raw materials and the standardization of finished herbal drugs because no single component contributes to the total efficacy. Thus, the chromatographic fingerprinting technique of TCM has proved to be a comprehensive strategy for assessing the intact quality of herbal medicine, since the origin of the herbal medicines could be identified and classified based on so-called phytoequivalence. On the other hand, chromatographic fingerprinting is essentially a high-throughput technique and an integral tool to explore the complexity of herbal medicines. In order to further control the comprehensive quality of TCMs, some strategies are proposed to trace the chemical changes of chromatographic fingerprints both in product processing and/or after their administration by modern chromatographic techniques and chemometrics. Combined with the techniques developed in systems biology, it seems also possible to reveal the working mechanism of TCMs and to further control their intrinsic quality. © Schweizerische Chemische Gesellschaft.

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