Zhong F.,Xinjiang Production and Construction Group |
Li N.,Xinjiang Production and Construction Group |
Huang X.,Xinjiang Production and Construction Group |
Guo Y.,Xinjiang Production and Construction Group |
And 4 more authors.
Virus Genes | Year: 2011
Whole blood samples (472) were collected during the period of 2006-2008 from 15 cattle farms in seven districts of the Xinjiang Uygur Autonomous Region, which is a major pastoral area and represents 1/6 of Chinese territory. Bovine viral diarrhea virus (BVDV) was detected by nested reverse transcription polymerase chain reaction assay (RT-nPCR) and sequenced within the 5′-untranslated region of the genome. Approximately 43% (202 samples) were BVDV-positive. Of the positive samples, 24 were chosen for further sequencing, sequence comparisons, and phylogenetic reconstructions. The phylogenetic reconstructions demonstrated that these isolates clustered into either BVDV1b (18 samples) or BVDV1c (six samples) subgenotypes. None of the isolates sequenced showed any cytopathic effect in culture. Unlike previously published studies in Chinese cattle, no BVDV2 genotypes were detected. These results indicate that the predominant subgenotypes in Xinjiang are BVDV1b and BVDV1c. This finding can be useful for further BVDV epidemiological study in China. © 2010 Springer Science+Business Media, LLC.
Tian Z.,North University of China |
Sutton B.J.,King's College London |
Zhang X.,North University of China |
Zhang X.,Xinjiang Production and Construction Group
Journal of Receptors and Signal Transduction | Year: 2014
The neonatal Fc receptor (FcRn) mediates the transfer of IgG and albumin, also protects them from catabolism. This study characterized the expression of FcRn in different organs of neonatal and pubertal rats by reverse transcription-PCR (RT-PCR) and immunohistochemistry, demonstrates that FcRn is expressed in liver, kidney, intestine, heart, lung, spleen, skin and skeletal muscles at varying levels post-gestation from d 1 to d 63. This finding is contrary to previous studies claiming that FcRn is undetectable in most tissues after weaning. Lungs were the predominant organs for FcRn expression, whereas skin, liver and intestine are considerably less expressed organs. The expression of FcRn fluctuated in all the organs tested, and with a higher frequency before weaning compared to puberty. These findings may provide clues for the better understanding of FcRn function, and are important for determining the dosage levels for IgG and the constant region fragment (Fc)-containing therapeutic proteins whose half-life is regulated by FcRn. © 2014 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted.
Zhang X.,Shihezi University |
Yao Z.,Shihezi University |
Zhao S.,Shihezi University |
Xie H.,Shihezi University |
Yang M.,Xinjiang Production and Construction Group
Journal of Phytopathology | Year: 2013
Stem rot was recorded on Orobanche aegyptiaca in Shihezi City, Xinjiang Uygur Autonomous Region, China from 2010 to 2011. The pathogen was isolated repeatedly from the infected stems and was identified as Rhizopus oryzae based on morphology, cultural features and molecular analysis. Koch's postulates were supported by pathogenicity tests conducted on healthy plants grown on processing tomato and melon. To our knowledge, this paper is the first to report the occurrence of R. oryzae stem rot on O. aegyptiaca. © 2013 Blackwell Verlag GmbH.
Fan H.,Shihezi University |
Zhang F.,Shihezi University |
Tan L.,Shihezi University |
Wang K.,Xinjiang Production and Construction Group
Research on Crops | Year: 2013
Increased planting density is one of the chief routes to increased cotton yield in Xinjiang. This paper relies on density experiments to analyze the relationship between aboveground biomass, canopy light interception and light utilization efficiency in high density cotton populations in Xinjiang, and clarifies the compensation effect achieved by dense planting in relation to cotton growth and yield. The results indicate that differences in the canopy light interception of cotton populations with different densities can be expressed primarily in terms of maximum light interception and its continuation time. The canopy light interception of high, medium, and low density cotton populations uniformly reaches a maximum value 80-90 days after sowing (flowering and boll stages). The light interception value can be maintained near maximum for a period of time in the case of low density populations, but falls rapidly from its peak in high density populations. Among cotton populations in the peak boiling stage, as planting density increases, the greatest light interception shifts from the lower parts of plants (15-30 cm) to the middle and upper parts of plants (30-60 cm). The extinction coefficient gradually increases with density, but does not vary significantly among the three densities. Different cotton populations have light extinction coefficients of 0.98 during the flowering and boll stages. Dense planting does not affect light utilization efficiency, and three cotton population densities all have a light utilization efficiency of 3.2 g/MJ.
Guan F.,China Jiliang University |
Pan L.,China Jiliang University |
Li J.,China Jiliang University |
Tang H.,Xinjiang Production and Construction Group |
And 2 more authors.
Virus Genes | Year: 2011
It is well known that scrapie is a fatal, neurodegenerative disease in sheep and goat, which belongs to the group of transmissible spongiform encephalopathies (TSEs) or prion diseases. It has been confirmed that the polymorphisms of prion protein gene (PRNP) at codons 136, 154, and 171 have strong relationship with scrapie in sheep. In the present study, nine polymorphisms of PRNP at codons 136, 154, and 171 and other six loci (at codons 101, 112, 127, 137, 138, and 152) were detected in 180 Chinese Hu sheep. All the alleles at codons 136, 154, and 171 have been identified and resulted in three new genotypes. The frequencies of predominant alleles were 85% (A136), 99.40% (R154), and 37.78% (Q171), respectively. The predominant haplotype ARQ has a relatively high frequency of 57.77%. The frequencies of dominant genotypes of ARR/ARQ and ARQ/ARQ were 30 and 26.67%, respectively. Three new found genotypes named ARQ/TRK, ARQ/TRR, and TRR/TRQ had the same lower frequencies (0.56%). The relationship of PRNP genotype with scrapie risk and litter size showed that the predominant genotypes are corresponded to the risk score of R1 (1.67%), R2 (32.22%), and R3 (42.22%). Just at the first parity, the individuals with ARH/ARH genotype had significantly larger litter size than the mean value and those with ARQ/ARQ and ARR/ARQ genotypes. In short, this study provided preliminary information about alleles and genotypes of PRNP in Chinese Hu sheep. It could be concluded that Hu sheep has a low susceptibility to natural scrapie, and the predominant PRNP genotype at least has no significant effect on litter size. © 2011 The Author(s).
Wang X.,Shihezi University |
Diao M.,Shihezi University |
Diao M.,Xinjiang Production and Construction Group |
Ma F.,Shihezi University |
And 4 more authors.
Nongye Jixie Xuebao/Transactions of the Chinese Society for Agricultural Machinery | Year: 2014
The leaf area index (LAI) and specific leaf area (SLA) simulation model of processing tomato with drip irrigation were developed based on the accumulated physiological development time after emergence (PDT). Then a simulation of leaf area, dry matter production and accumulation of processing tomato with drip irrigation was developed based on physiological and ecological processes of photosynthesis and dry matter production simulation model. The results showed that when using the model based on PDT, the coefficient of determination (R2), root mean squared error (RMSE) and modelling efficiency indexes (ME) between simulated and measured leaf area index based on the 1:1 line were 0.9265, 12.87% and 0.9724, respectively. However, when using the model based on SLA, the R2, RMSE and ME between simulated and measured LAI based on the 1:1 line were 0.6758, 42.24%, and 0.7124, respectively. When using the model based on PDT, the R2, RMSE and ME between simulated and measured aboveground dry matter weight based on the 1:1 line were 0.9903, 11.91% and 0.9901, respectively. However, when using the model based on SLA, the R2, RMSE and ME between simulated and measured aboveground dry matter weight based on the 1:1 line were 0.8956, 31.29% and 0.7504, respectively. Compared with the SLA method, PDT method to improve the processing tomato leaf area index prediction accuracy while also improving the prediction accuracy of the aboveground dry matter weight.
Li R.-Y.,Shihezi University |
Peng Q.,Shihezi University |
Jia B.,Shihezi University |
Shi G.-Q.,Xinjiang Production and Construction Group |
And 3 more authors.
Parasitology Research | Year: 2011
Different MHC haplotype of Kazakh sheep has different resistance and susceptibility of hydatidosis. Notably, the MvaIbc-SacIIab-Hin1Iab haplotype of MHC-DRB1 exon two was associated with resistance hydatidosis. In order to analyze the antibody and cytokine responses to hydatidosis in Kazakh sheep with hydatidosis resistance haplotype, eight Kazakh sheep with the haplotype of MvaIbc-SacIIab-Hin1Iab were chosen as the test group, and other eight, which were not associated with hydatidosis resistance or susceptibility, were taken as control. After experimentally infected with hydatid orally, the blood was collected on 0, 7, 14, 30, 45, 60, 75, 90, 105, and 120 days. Serum and mRNA level of the cytokines IL-2, IFN-γ, TNF-α, IL-4, and IL-10 were evaluated by ELISA and fluorescence quantitative real-time polymerase chain reaction, respectively. The total white blood cells and leukomonocytes were determined by automation cytoanalyze. The level of IgE, IgG, and IgM were evaluated by ELISA. The results showed that the total white blood cells and leukomonocytes in test group were significantly higher than in control on 7, 45, 90, and 105 days post-infection (p.i.). The serum level of IL-2 in test group was significantly higher than in control on 45 days p.i., while the difference of IL-2 mRNA expression between test and control group was not significant. The serum level of TNF-α in test group was significantly higher than in control at 90 and 105 days p.i., and the TNF-α mRNA in test group was also significantly higher than in control on 90 days p.i. The level of IgE, IgG, and IgM in test group was higher than in control, but none was significant. The results suggested that the test group, which was predominant of Th1, could induce the protective immunity, while the control, which was predominant of Th2, could induce the susceptibility to infection of hydatidosis. © 2010 Springer-Verlag.
Wang X.,Shihezi University |
Ma F.-Y.,Shihezi University |
Ma F.-Y.,Xinjiang Production and Construction Group |
Diao M.,Shihezi University |
And 6 more authors.
Chinese Journal of Applied Ecology | Year: 2014
Three field experiments were conducted to simulate the dynamics of aboveground biomass, N accumulation and utilization of drip-irrigated processing tomatoes at different N fertilization rates (0, 75, 150, 300, 450, or 600 kg·hm-2). The results showed that Logistic models best described the changes in aboveground biomass, N accumulation, and utilization of accumulated N efficiency with the physiological development time (PDT). Rapid accumulation of N began about 4-6 d (PDT) earlier than the rapid accumulation of aboveground biomass. The momentary utilization rate of N (NMUR) increased after emergence, reached a single peak, and then decreased. The N accumulation, aboveground biomass and yield were highest in the 300 kg·hm-2 treatment. The quadratic model indicated that application rate of 349 to 382 kg N·hm-2 was optimum for dripirrigated processing tomatoes in northern Xinjiang.
Chen C.,Shaanxi University of Technology |
Ku C.,No.1 Senior School in Lushi County |
Bo X.,Xinjiang Production and Construction Group |
Wang X.,Xinjiang Production and Construction Group
Biotechnology Letters | Year: 2014
A novel antimicrobial peptide, SRTAP-40 has been purified and characterized from sheep reproductive tract. The isolation procedure entailed acetic acid extraction, gel filtration chromatography, and HPLC. SRTAP-40 is composed of 40 amino acid residues with a MW of 4,820.47 Da from MALDI-TOF–MS. Its N-terminal sequence was AYVLDEPKP. SRTAP-40 cDNA was cloned by 3′-RACE. SRTAP-40 showed activity against E. coliStaphylococcus aureus,Streptococcus sp. and, Candida albicans with MIC values of 12, 12, 24, 6 μg/ml, respectively. By BLAST search, SRTAP-40 had no significant similarity to any known peptide. © 2014, Springer Science+Business Media Dordrecht.
Yao L.J.,Northwest University, China |
Shen Y.Y.,Northwest University, China |
Zhan J.P.,Northwest University, China |
Xu W.,Northwest University, China |
And 3 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2012
During a study of the diversity and phylogeny of rhizobia in the root nodules of Kummerowia striata grown in north-western China, four strains were classified in the genus Rhizobium on the basis of their 16S rRNA gene sequences. The 16S rRNA gene sequences of three of these strains were identical and that of the other strain, which was the only one isolated in Yangling, differed from the others by just 1 bp. The16S rRNA gene sequences of the four strains showed a mean similarity of 99.3% with the most closely related, recognized species, Rhizobium vitis. The corresponding recA and glnA gene sequences showed similarities with established species of Rhizobium of less than 86.5% and less than 89.6%, respectively. These low similarities indicated that the four strains represented a novel species of the genus Rhizobium. The strains were also found to be distinguishable from the closest related, established species (R. vitis) by rep-PCR DNA fingerprinting, analysis of cellular fatty acid profiles and from the results of a series of phenotypic tests. The level of DNA-DNA relatedness between the representative strain CCNWSX 0483T and Rhizobium vitis IAM 14140T was only 40.13%. Therefore, a novel species, Rhizobium taibaishanense sp. nov., is proposed, with strain CCNWSX 0483T (5ACCC 14971T5HAMBI 3214T) as the type strain. In nodulation and pathogenicity tests, none of the four strains of Rhizobium taibaishanense sp. nov. was able to induce any nodule or tumour formation on plants. As no amplicons were detected when DNA from the strains was run in PCR with primers for the detection of nodA, nifH and virC gene sequences, the strains probably do not carry sym or vir genes. © 2012 IUMS.