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Dong X.,Beijing University of Chinese Medicine | Lan W.,Beijing University of Chinese Medicine | Lan W.,Xinjiang Key Laboratory of Famous Prescription and Science of Formulas | Yin X.,Beijing University of Chinese Medicine | And 3 more authors.
Evidence-based Complementary and Alternative Medicine | Year: 2017

A simple and sensitive HPLC-UV method has been developed for the simultaneous determination of quercetin, luteolin, and apigenin in rat plasma after oral administration of Matricaria chamomilla L. extract. The flow rate was set at 1.0 ml/min and the detection wavelength was kept at 350 nm. The calibration curves were linear in the range of 0.11-11.36 μg/ml for quercetin, 0.11-11.20 μg/ml for luteolin, and 0.11-10.60 μg/ml for apigenin, respectively. The intraday and interday precisions (RSD) were less than 8.32 and 8.81%, respectively. The lower limits of quantification (LLOQ) of the three compounds were 0.11 μg/ml. The mean recoveries for quercetin, luteolin, and apigenin were 99.11, 95.62, and 95.21%, respectively. Stability studies demonstrated that the three compounds were stable in the preparation and analytical process. The maximum plasma concentration (Cmax) was 0.29 ± 0.06, 3.04 ± 0.60, and 0.42 ± 0.10 μg/ml, respectively. The time to reach the maximum plasma concentration (Tmax) was 0.79 ± 0.25, 0.42 ± 0.09, and 0.51 ± 0.13 h, respectively. The validated method was successfully applied to investigate the pharmacokinetics study of quercetin, luteolin, and apigenin in rat plasma after oral administration of M. chamomilla extract. © 2017 Xiaoxv Dong et al.


Liu F.,Xinjiang Normal University | Liu W.,Xinjiang Medical University | Ding W.,Xinjiang Key Laboratory of Famous Prescription and Science of Formulas | Lv G.,Xinjiang Key Laboratory of Famous Prescription and Science of Formulas | Zhou X.,Xinjiang Medical University
Asian Journal of Chemistry | Year: 2014

The concentration of twelve trace elements (Ca, Cd, Cr, Cu, Fe, Hg, K, Mg, Mn, Na, Pb, Zn) in eight Medicago sativa L. seeds samples, which were uygur medicine and carried out by inductively coupled plasma optical emission spectrometry (ICP-OES). A diluted oxidant mixture (2 mL HNO3 + 1 mL H2O2 + 3 mL H2O) was used to achieve the complete decomposition of the organic matrix in a closed-vessel microwave oven. The accuracy of the entire proposed method was confirmed by standard reference material analysis. Twelve trace elements contents were found detected at in different Medicago sativa L. seeds samples. The results in the samples (minimum-maximum in ìg/g) were : Ca 0.022-0.058, Cr 0.126-7.106, Cu 0.570-4.504, Fe 8.004-12.588, Mn 0.035-0.054, Ni 0.908-2.182, Pb 0.099-0.134 and Zn 2.206-8.982, respectively. The trace elements analysis method of the ICP-OES was found to be fast, reliable, simple and excellent in determination of the trace elements.


Liu F.,Xinjiang Normal University | Tian S.,Xinjiang Key Laboratory of Famous Prescription and Science of Formulas | Tian S.,Xinjiang Medical University
Research Journal of Chemistry and Environment | Year: 2013

An ultrasound-assisted extraction (UAE) and highperformance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method that offers good linearity, accuracy and precision was developed for the simultaneous determination of rosmarinic acid (RA) in Cordia dichotoma seeds. Single-factor and orthogonal experiments were performed to determine the appropriate range of extraction conditions and to obtain the optimum extraction conditions respectively. The optimum UAE conditions for C. dichotoma seeds were as follows: ethanol concentration of 70%, solid/liquid ratio of 1:35 g/mL, ultrasound time of 30 min and ultrasound power of 300 W. UAE is an alternative technique for the fast extraction of C. dichotoma seeds. The factors selected in this study had significant functions on the extraction of RA from C. dichotoma seeds.


PubMed | Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Xinjiang Medical University and Xinjiang Normal University
Type: Journal Article | Journal: Pakistan journal of pharmaceutical sciences | Year: 2014

This study aims to determine the phytochemical composition and antioxidant activity of air-dried Cordia dichotoma seeds. Total polyphenolic content was analyzed via the Folin-Ciocalteu method. Total triterpenoid content and amino acids was analyzed colorimetrically. The rosmarinic acid content was examined using high-performance liquid chromatography tandem mass spectrometry. The ethanolic extracts contained polyphenolic compounds (1.0%), triterpenoids (0.075%), amino acids (1.39%), and rosmarinic acid (0.0028%). The results from this study indicate that C. dichotoma seeds are a rich source of polyphenolic compounds and amino acids, which can be used for quality assessment. The ethanolic extract of C. dichotoma seeds has good antioxidant capacity.


PubMed | Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Xinjiang Medical University and Xinjiang Normal University
Type: Journal Article | Journal: Pharmacognosy magazine | Year: 2015

Given the high-effectiveness and low-toxicity of abnormal savda munziq (ASMQ), its herbal formulation has long been used in traditional Uyghur medicine to treat complex diseases, such as cancer, diabetes, and cardiovascular diseases.ASMQ decoction by reversed-phase high-performance liquid chromatography coupled with a diode array detector was successfully developed for the simultaneous quality assessment of gallic acid, protocatechuic acid, caffeic acid, rutin, rosmarinic acid, and luteolin. The six phenolic compounds were separated on an Agilent TC-C18 reversed-phase analytical column (4.6 250 mm, 5 m) by gradient elution using 0.3% aqueous formic acid (v/v) and 0.3% methanol formic acid (v/v) at 1.0 mL/min.The plant material was separately ground and mixed at the following ratios (10): Cordia dichotoma (10.6), Anchusa italic (10.6), Euphorbia humifusa (4.9), Adiantum capillus-veneris (4.9), Ziziphus jujube (4.9), Glycyrrhiza uralensis (7.1), Foeniculum vulgare (4.9), Lavandula angustifolia (4.9), Dracocephalum moldavica L. (4.9), and Alhagi pseudoalhagi (42.3).The precisions of all six compounds were <0.60%, and the average recoveries ranged from 99.39% to 104.85%. Highly significant linear correlations were found between component concentrations and specific chromatographic peak areas (R (2) > 0.999).The proposed method was successfully applied to determine the levels of six active components in ASMQ.Given the simplicity, precision, specificity, and sensitivity of the method, it can be utilized as a quality control approach to simultaneously determining the six phenolic compounds in AMSQ.


Tian S.,Xinjiang Key Laboratory of Famous Prescription and Science of Formulas | Tian S.,Xinjiang Medical University | Yu Q.,Xinjiang Key Laboratory of Famous Prescription and Science of Formulas | Yu Q.,Xinjiang Medical University | And 5 more authors.
Natural Product Communications | Year: 2012

An efficient and accurate fingerprinting method using reversed-phase rapid-resolution liquid-chromatography coupled with photodiode array detection has been developed and optimized to examine the variance in active compounds among Ziziphora clinopodioides Lam from different locations. Three active components, diosmin, linarin and pulegone, were identified by matching their retention times and UV spectra with the corresponding reference compounds. Our results indicated that chromatographic fingerprints, in combination with principal component analysis (PCA) and hierarchical clustering analysis (HCA), could efficiently identify and distinguish Z. clinopodioides from different sources. Our fingerprinting methods and data will be useful for quality control, and thus, more effective dosing in clinical application of Z. clinopodioides.


Gong H.Y.,Xinjiang Key Laboratory of Famous Prescription and Science of Formulas | Gong H.Y.,Xinjiang Medical University | Ma X.M.,Xinjiang Medical University | Tian S.G.,Xinjiang Key Laboratory of Famous Prescription and Science of Formulas
E-Journal of Chemistry | Year: 2012

Paeonia sinjiangensis K. Y. Pan is a perennial herb belonging to the family Ranunculaceae which is one of the most important crude drugs in traditional Chinese medicine, used as an anti-inflammatory, analgesic and sedative agent. This paper deals with the detailed pharmacognostical evaluation of the crude drug P. sinjiangensis K. Y. Pan. The microscopic, physico-chemical, preliminary physicochemical parameters, total phenols contents presented in this paper may be proposed as parameters to establish the authenticity of P. sinjiangensis K. Y. Pan and can possibly help to differentiate the drug from its other species.


PubMed | Xinjiang Key Laboratory of Famous Prescription and Science of Formulas
Type: Journal Article | Journal: Pharmacognosy magazine | Year: 2010

A rapid, effective, binary reverse phase rapid resolution liquid chromatographic method has been developed for the determination of Paeoniflorin extracted from Paeonia sinjiang K. Y. Pan. with short run time. RRLC separation was achieved by using Agilent (Zorbas XDB-C(18) 4.6 mm x 50 mm, 1.8 mum) column with mobile phase complosed of methanol and 0.05 mol/l potassium phosphate monobasic. Flow rate was at 1.0 ml/min. Retention time was at about 1.16 min. Recovery rate was 99.8% and relative standard deviation of three replicate samples tested was 1.8%. RRLC method has been applied to both in vitro studies of paeoniflorin formulation and drug estimation in biological samples.


PubMed | Xinjiang Key Laboratory of Famous Prescription and Science of Formulas
Type: Journal Article | Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica | Year: 2014

In this paper, microscopic identification method was adopted to observe the microscopic characters of ten batches of Medicago sativa seeds. And M. sativa seeds were identificated by TLC method in contrast to trigonelline and stachydrine hydrochloride. The impurities, moisture, ash, sour insoluble ash were detected based on Chinese Pharmacopoeia 2010 version (Vol I ). An HPLC method was also established for determination of trigonelline in the M. sativa seeds. The contents of impurities, moisture, ash, sour insoluble ash should not exceed 5%, 10%, 6%, and 2%, respectively. The content of trigonelline should be not less than 0.795 6 mg x g(-1). The experimental methods were accurate and reliable, and can be used as the quality control of the seeds of M. sativa.


PubMed | Xinjiang Key Laboratory of Famous Prescription and Science of Formulas
Type: Journal Article | Journal: Natural product communications | Year: 2012

An efficient and accurate fingerprinting method using reversed-phase rapid-resolution liquid-chromatography coupled with photodiode array detection has been developed and optimized to examine the variance in active compounds among Ziziphora clinopodioides Lam from different locations. Three active components, diosmin, linarin and pulegone, were identified by matching their retention times and UV spectra with the corresponding reference compounds. Our results indicated that chromatographic fingerprints, in combination with principal component analysis (PCA) and hierarchical clustering analysis (HCA), could efficiently identify and distinguish Z. clinopodioides from different sources. Our fingerprinting methods and data will be useful for quality control, and thus, more effective dosing in clinical application of Z. clinopodioides.

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