Xinjiang Fisheries Research Institute

Urunchi, China

Xinjiang Fisheries Research Institute

Urunchi, China

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Wang J.,Huazhong Agricultural University | Zhou Q.,Huazhong Agricultural University | Xie C.,Huazhong Agricultural University | Li H.,Xinjiang Fisheries Research Institute | Cai L.,Xinjiang Fisheries Research Institute
Research of Environmental Sciences | Year: 2015

The Benthic-Index of Biotic Integrity (B-IBI) was applied to evaluate the health state of Irtysh River drainage in Xinjiang Uygur Autonomous Region, in order to build an assessment criteria and standard of ecosystem health for cross-border rivers in northwestern China. Twenty-three candidate metrics of benthic macroinvertebrates were selected from 10 typical sampling sites from Irtysh River sampled in July, August, October 2012 and May 2013. Then, five key metrics were detected and entered into the final B-IBI system, including the number of the total taxa, the number of EPT (Ephemeroptera, Plecoptera, Trichoptera), the three-dominant taxa percentage, the Plecoptera percentage and the clingers' percentage. Based on 25% percentile of B-IBI value in reference sampling sites, the assessment grades were as follows: B-IBI>2.04 represents excellent, 1.53


Bao M.,Huazhong Agricultural University | Zhang X.,Huazhong Agricultural University | Xie C.,Huazhong Agricultural University | Wan S.,Huazhong Agricultural University | And 2 more authors.
Mitochondrial DNA | Year: 2016

The complete mitochondrial genome of Schizothorax pseudaksaiensis was cloned and sequenced in the present study. The genome was 16,582 bp in size, which had a mostly conserved structural organization in comparison with that of other teleost fish. It consisted of 37 genes (13 protein-coding genes, 22 transfer RNA genes and 2 ribosomal RNA genes), and 2 main non-coding regions (the control region and the origin of the light strand replication). All protein-coding genes started with ATG except for COX1, which began with GTG. However, the termination codons of 13 protein-coding genes varied with TAA, TA, T or TAG. The overall base composition of S. pseudaksaiensis in descending order was A 30.18%, C 27.08%, T 25.37% and G 17.37%, with a slight A + T bias. The complete mitochondrial genome sequence may provide useful information for phylogenetic analysis and studies of population genetics of S. pseudaksaiensis. © 2014 Informa UK Ltd.


Yang T.,Xinjiang Fisheries Research Institute | Yang T.,Ocean University of China | Meng W.,Xinjiang Fisheries Research Institute | Zhang R.,Xinjiang Fisheries Research Institute | And 4 more authors.
Russian Journal of Genetics | Year: 2016

DNA barcoding was a molecular diagnostic method that provided rapid and accurate species identification. The 650 bp-length cytochrome c oxidase subunit I (COI) gene of 33 species in Irtysh River China was sequenced and analyzed in this study. The average intra-species, -genus, -family, and -order of Kimura two parameter (K2P) distances were 0.003, 0.060, 0.163 and 0.240, respectively. The genetic distance between genus Barbatula and Cobitis was the largest whereas that between genus Hypophthalmichthys and Aristichthys was the smallest. The neighbour-joining tree constructed by all 44 haplotypes was divided into two major clusters: Cypriniformes fishes and other fishes. A cryptic species of Barbatula barbatula was detected according to 2% genetic threshold. © 2016, Pleiades Publishing, Inc.


Lei D.,Huazhong Agricultural University | Conteh Kanu U.,Huazhong Agricultural University | Zhao G.,Huazhong Agricultural University | Xie P.,Huazhong Agricultural University | And 4 more authors.
Mitochondrial DNA | Year: 2015

Based upon the morphological characters, the genus Triplophysa (plateau Loach) is a highly diverse group in the family Balitoridae (or Cypriniformes, Cobitoidea) with 133 valid species. Therefore, the taxonomic relationship of this species at the genetic level remains ambiguous. In the present study, we sequenced the complete mitochondrial genome of Triplophysa dorsalis. In order to understand its position and genetic background at the gene level, the characteristics of mitochondrial DNA sequences and phylogenetic relationship were examined. The mitochondrial genome of T. dorsalis is similar to those of the typical vertebrates, 16 572 bp in length, including 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and a non-coding control region (D-loop). All genes were encoded on the heavy strand except for ND6 and 8 tRNA genes. The overall base composition of the heavy strand was 28.16%, 28.41%, 25.62% and 17.82% for A, T, C and G, respectively. Phylogenetic analyses showed that the seven Triplophysa species clustered together with T. dorsalis to form a sister group with Triplophysa strauchii, T. bleekeri, T. stoliczkai and T. bombifrons. The two genera Triplophysa and Barbatula formed a sister-group relationship, the species Homatula potanini located in the intermediate position, and the genus Leptobotia elongata was in the basal position in the subfamily Nemacheilidae. Further investigations with more Triplophysa species need to be performed for better understanding of the evolutionary history of this fascinating genus. © 2015 Taylor & Francis.


Yang T.,Xinjiang Fisheries Research Institute | Yang T.,Ocean University of China | Meng W.,Xinjiang Fisheries Research Institute | Chen P.,Xinjiang Fisheries Research Institute | Gao T.,Ocean University of China
Mitochondrial DNA | Year: 2015

The complete nucleotide sequence of Gymnodiptychus dybowskii mitogenome (16 677 bp) has been determined, containing 13 protein-coding genes, two ribosomal RNA genes, 22 tRNA genes, and one non-coding control region. The base composition is 28.04% A, 26.92% T, 18.94% G, 26.10% C, with an AT bias of 54.97%. The origin of light-strand replication (OL) is found between tRNAAsn and tRNACys, which has the potential to fold in a step-loop secondary structure with a stem formed by 11 pairs of nucleotides and a loop of 14 nucleotides. The phylogenetic analysis indicates close relationship between genus Gymnodiptychus and Diptychus. © 2015 Taylor & Francis.


Zhao X.,Huazhong Agricultural University | Hu S.,Huazhong Agricultural University | Xie P.,Huazhong Agricultural University | Ao M.,Huazhong Agricultural University | And 3 more authors.
Mitochondrial DNA | Year: 2015

In this study, we cloned and sequenced the complete mitochondrial genome of Barbatula nuda. The genome was found to be 16,619 bp in size with a mostly conserved structural organization when compared with that of other teleost fish. It contained 37 genes (13 protein-coding genes, 22 transfer RNA genes, and 2 ribosomal RNA genes) and 2 main non-coding regions (the control region and the origin of the light-strand replication). All protein-coding genes are initiated with ATG except for COX 1, which begin with GTG instead. However, the termination codons of 13 protein-coding genes varied with TAA, TA, T or TAG. The complete mitochondrial genome sequence is useful for phylogenetic analysis and studies of population genetics of B. nuda. © 2013 Informa UK Ltd.


Hu S.,Huazhong Agricultural University | Niu J.,Xinjiang Fisheries Research Institute | Xie P.,Huazhong Agricultural University | Liu C.,Huazhong Agricultural University | And 3 more authors.
Mitochondrial DNA | Year: 2015

The complete mitochondrial genome of Leuciscus leuciscus baicalensis was cloned and sequenced in the present study. The genome which had a mostly conserved structural organization in comparison with that of other teleost fish was 16,606 bp in size. It consisted of 37 genes (13 protein-coding genes, 22 transfer RNA genes and 2 ribosomal RNA genes), and 2 main non-coding regions (the control region and the origin of the light strand replication). All protein-coding genes started with ATG except for COX 1, which began with GTG. However, the termination codons of 13 protein-coding genes varied with TAA, TA, T or TAG. The complete mitochondrial genome sequence provided useful information for phylogenetic analysis and studies of population genetics of L. leuciscus baicalensis. © 2014 Informa UK Ltd.


Wang F.,Huazhong Agricultural University | Niu J.,Xinjiang Fisheries Research Institute | Hu S.,Huazhong Agricultural University | Xie P.,Huazhong Agricultural University | And 4 more authors.
Mitochondrial DNA | Year: 2016

In this study, we determined the complete nucleotide sequence of the mitochondrial genome for a cold water fish, Leuciscus idus. The complete mitochondrial genome was sequenced and analyzed using the primer walking method and 30 pairs of newly designed primers were used. Direct sequencing of the PCR products demonstrated that the genome contained the same 37 mitochondrial genes (two ribosomal RNA, 22 transfer RNA and 13 protein-coding genes) and two main non-coding regions (the control region and the origin of the light strand replication) as those found in other vertebrates. Nearly all the protein-coding genes start codons were ATG except COX 1, meanwhile, the termination codons of which varied with TAA, TA, T, or TAG. © 2014 Informa UK Ltd.


Xie P.,Huazhong Agricultural University | Ao M.,Huazhong Agricultural University | Liu C.,Huazhong Agricultural University | Zhang Z.,Huazhong Agricultural University | And 4 more authors.
Mitochondrial DNA | Year: 2016

As reported, previous studies of Phoxinus phoxinus ujmonensis (Cypriniformes: Cyprinidae) have focused primarily on morphology and ecology. In this study, we cloned and sequenced the complete nucleotide sequence of the mitochondrial genome for P. phoxinus ujmonensis. The mitogenome was 17,738 bp in length, including 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and 1 control region. All genes were encoded on the heavy strain except for ND6 and eight tRNA genes. The overall base composition of the heavy strain was 28.50% for A, 28.24% for T, 25.32% for C and 17.94% for G. The control region was revealed to contain tandem repeats. The mitogenome data of P. phoxinus ujmonensis should contribute to phylogenetic analysis and studies of population genetics of Phoxinus fishes. © 2014 Informa UK Ltd.


Ao M.,Huazhong Agricultural University | Xie P.,Huazhong Agricultural University | Zhao G.,Huazhong Agricultural University | Guo F.,Huazhong Agricultural University | And 2 more authors.
Mitochondrial DNA | Year: 2016

In this study, the complete sequence of the 16,527 base pairs Cottus sibiricus altaicus mitochondrial genome was presented. The composition of the whole mitochondrial DNA was 26.93% for A, 26.30% for T, 29.70% for C, 17.07% for G. The genes found in C. sibiricus altaicus and other vertebrate mtDNA were the same, which contained the genes for 13 proteins, 22 transfer RNAs, 2 ribosomal RNAs and 2 main non-coding genes (the control region (CR) and the origin of the light strand replication (OL). The sequence showed high economy of organization in that there were few nucleotides between individual genes except for the control region. © 2014 Informa UK Ltd.

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