Zhu X.-L.,Xinjiang Agricultural University
Chang'an Daxue Xuebao (Ziran Kexue Ban)/Journal of Chang'an University (Natural Science Edition) | Year: 2010
Considering the uncertainty of highway geometry design parameters, the reliability models involved in stop sight distance (SSD), horizontal curve radius (HCR) and vertical curve radius (VCR) are developed based on probability theory. Reliability indexes and failure probability are obtained by reliability calculation method and are used to analyze reliability of highway alignment design indicators. The study results show that the reduction of stop sight distance (SSD) should be limited in the range of 15 percent at any point in highway to guarantee the reliability of SSD. The declination of lateral friction factors significantly affects the reliability of HCR. If the reduction of VCR exceeds a certain value, the VCR reliability will sharply decline. Reliability theory in highway alignment design provide theoretical basis for the designers who are confused about the best choice between safety and cost for any factor. Meanwhile, it offers the designers a new design idea.
Guan Q.,University of Maryland University College |
Lu X.,Xinjiang Agricultural University |
Zeng H.,University of Maryland University College |
Zhang Y.,University of Maryland University College |
Zhu J.,University of Maryland University College
Plant Journal | Year: 2013
Summary microRNAs (miRNAs) play important roles in plant growth and development. Previous studies have shown that down-regulation of miR398 in response to oxidative stress permits up-regulation of one of its target genes, CSD2 (copper/zinc superoxide dismutase), and thereby helps plants to cope with oxidative stress. We report here that heat stress rapidly induces miR398 and reduces transcripts of its target genes CSD1, CSD2 and CCS (a gene encoding a copper chaperone for both CSD1 and CSD2). Transgenic plants expressing miR398-resistant forms of CSD1, CSD2 and CCS under the control of their native promoters are more sensitive to heat stress (as indicated by increased damage at the whole-plant level and to flowers) than transgenic plants expressing normal coding sequences of CSD1, CSD2 or CCS under the control of their native promoters. In contrast, csd1, csd2 and ccs mutant plants are more heat-tolerant (as indicated by less damage to flowers) than the wild-type. Expression of genes encoding heat stress transcription factors (HSF genes) and heat shock proteins (HSP genes) is reduced in heat-sensitive transgenic plants expressing miR398-resistant forms of CSD1, CSD2 or CCS but is enhanced in the heat-tolerant csd1, csd2 and ccs plants. Chromatin immunoprecipitation assays revealed that HSFA1b and HSFA7b are the two HSFs responsible for heat induction of miR398. Together, our results suggest that plants use a previously unrecognized strategy to achieve thermotolerance, especially for the protection of reproductive tissues. This strategy involves the down-regulation of CSD genes and their copper chaperone CCS through heat-inducible miR398. © 2013 The Authors The Plant Journal © 2013 John Wiley & Sons Ltd.
Zhu X.,Xinjiang Agricultural University
ICCMS 2010 - 2010 International Conference on Computer Modeling and Simulation | Year: 2010
An accurate mathematical model for describing traffic accidents is difficult to be constructed due to various factors such as humans, vehicles and environments. To achieve a better estimation of traffic crashes, a novel composite grey BP neural network(CGBNN) model is presented in this paper. First, the original predicted values of traffic accidents are separately obtained by the GM(1,1) model, the Verhulst model and the DGM(2,1) model. Then, a CGBNN model is constructed by fusing the advantages of the grey models and the BNN model to improve the forecasting precision of the original grey models, the reasonable weights of the neural networks are acquired by an iterative training and learning process. The results of the CGBNN model on predicting real-world traffic fatalities show that the forecasting accuracy is much enhanced when the proposed method is applied. © 2010 IEEE.
Su Y.,Xinjiang Agricultural University
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2012
In order to compare the infection of HIV-1 pseudovirus to suspended and adherent cells, Tzmbl cells containing beta-gal (beta-galactosidase) reporter gene were used here to do the analysis. pseudoviruses were generated by co-transfection of 293T cells with the plasmid pNL43R-E- and HIV envelope expressing plasmid. Supernatant of co-transfected 293T cells was collected and used to infect Tzmbl cells with or without trypsin treatment. Forty-eight hours after infection, beta-gal positive Tzmbl cells and virus infection were determined using X-gal staining and beta-glo (beta-galactosidase) assay. The efficiency of HIV pseudoviruses infection of suspended Tzmbl cell was higher than that of adherent cells and the increase of infection correlated with the pseudoviral subtype. This study may provide a useful method for HIV biological study and neutralization assays using a single-round replicative pseudovirus in the future.
Zhao J.,Xinjiang Agricultural University
Proceedings - 2016 8th International Conference on Measuring Technology and Mechatronics Automation, ICMTMA 2016 | Year: 2016
English for science and technology(EST) is an important way to describe scientific work, and flexible means(FM) is the generally accepted method to translate EST. In this paper, we evaluate the application of flexible means in EST translation(ESTT) via the explicit questionnaire. Directly employing the principal component analysis(PCA), we obtain the relative importance of seven different kinds of FM. Based on analytic hierarchy process(AHP), we take three kinds of EST including biological EST(BEST), agricultural EST(AEST) and medical EST(MEST) as our objects to investigate the application of FM, with the seven FM being criterion layer and the three EST being scheme layer. We find that, the application of FM in BEST is the most common among the three EST. © 2016 IEEE.