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Liu X.,Shandong University | Zhao Y.,Shandong University | Sun B.,Xinjiang Academy of Agriculture Science | Liu C.,Xinjiang Academy of Agriculture Science | And 2 more authors.
Plant, Cell and Environment | Year: 2013

In this study, the ZmPIS gene with a maize ubiquitin promoter was introduced into maize (Zea mays L.) inbred line DH4866 by an Agrobacterium-mediated method to explore the function of the ZmPIS gene in the response of maize to drought stress. The overexpression of ZmPIS in maize resulted in significantly elevated levels of most phospholipids, galactolipids in leaves compared with those in WT and markedly up-regulated expression of some genes involved in the phospholipids metabolism pathway and the ABA biosynthesis pathway after drought stress. Consistent with these results, the drought stress tolerance of the ZmPIS sense transgenic plants were enhanced significantly compared to WT maize plants. These results imply that ZmPIS regulate the plant response to drought stress through altering membrane lipid composition and increasing ABA synthesis in maize. Phosphatidylinositol (PtdIns) synthase is a key enzyme in the phospholipid pathway and catalyses the formation of PtdIns. PtdIns is not only a structural component of cell membranes, but also the precursor of the phospholipid signal molecules that regulate plant response to environment stresses. Here, we obtained transgenic maize constitutively overexpressing or underexpressing PIS from maize (ZmPIS) under the control of a maize ubiquitin promoter. Transgenic plants were confirmed by PCR, Southern blotting analysis and real-time RT-PCR assay. The electrospray ionization tandem mass spectrometry (ESI-MS/MS)-based lipid profiling analysis showed that, under drought stress conditions, the overexpression of ZmPIS in maize resulted in significantly elevated levels of most phospholipids and galactolipids in leaves compared with those in wild type (WT). At the same time, the expression of some genes involved in the phospholipid metabolism pathway and the abscisic acid (ABA) biosynthesis pathway including ZmPLC, ZmPLD, ZmDGK1, ZmDGK3, ZmPIP5K9, ZmABA1, ZmNCED, ZmAAO1, ZmAAO2 and ZmSCA1 was markedly up-regulated in the overexpression lines after drought stress. Consistent with these results, the drought stress tolerance of the ZmPIS sense transgenic plants was enhanced significantly at the pre-flowering stages compared with WT maize plants. These results imply that ZmPIS regulates the plant response to drought stress through altering membrane lipid composition and increasing ABA synthesis in maize. © 2012 Blackwell Publishing Ltd.


Lidbury I.,University of Warwick | Krober E.,University of Warwick | Zhang Z.,Xinjiang Academy of Agriculture science | Zhu Y.,University of Warwick | And 3 more authors.
Environmental Microbiology | Year: 2016

The volatile organosulfur compound, dimethylsulfide (DMS), plays an important role in climate regulation and global sulfur biogeochemical cycles. Microbial oxidation of DMS to dimethylsulfoxide (DMSO) represents a major sink of DMS in surface seawater, yet the underlying molecular mechanisms and key microbial taxa involved are not known. Here, we reveal that Ruegeria pomeroyi, a model marine heterotrophic bacterium, can oxidize DMS to DMSO using trimethylamine monooxygenase (Tmm). Purified Tmm oxidizes DMS to DMSO at a 1:1 ratio. Mutagenesis of the tmm gene in R. pomeroyi completely abolished DMS oxidation and subsequent DMSO formation. Expression of Tmm and DMS oxidation in R. pomeroyi is methylamine-dependent and regulated at the post-transcriptional level. Considering that Tmm is present in approximately 20% of bacterial cells inhabiting marine surface waters, particularly the marine Roseobacter clade and the SAR11 clade, our observations contribute to a mechanistic understanding of biological DMSO production in surface seawater. © 2016 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd


Gao Y.,Shihezi University | Gao Y.,Xinjiang Academy of Agriculture Science | Lou K.,Xinjiang Academy of Agriculture Science | Li C.,Shihezi University
Nongye Gongcheng Xuebao/Transactions of the Chinese Society of Agricultural Engineering | Year: 2011

The effects of exogenous glycine betaine on the physiological responses of cotton seedings under salt stress were studied. No.18 cotton widely cultivated in Xinjiang was selected as study object under 200 mmol/L NaCL stress and spraying 5 mmol/L, 10 mmol/L glycine betaine respectively. The changes of physiological index were measured after 7 day. The results showed that under the condition of no salt stress, the content of proline and soluble sugar were improved significantly, while malonaldehyde (MDA) and antioxidative enzyme were not affected by spraying glycine betaine.The content of MDA increased significantly and proline, soluble sugar and antioxidative enzymes activity increased under salt stress. After spraying glycine betaine under salt stress, the production of MDA were inhibitted effectually, at the same time the content of proline, soluble sugar and antioxidative enzyme increased further more. Glycine betaine alleviated the damage of cotton seedings caused by salt tress, and 5 mmol/L glycine betaine had better effect.


Shi Y.,Xinjiang Academy of Agriculture Science | Shi Y.,Shihezi University | Lou K.,Xinjiang Academy of Agriculture Science | Li C.,Beijing Institute of Technology
Symbiosis | Year: 2011

An endophytic bacteriumn identified as Acinetobacter johnsonii strain 3-1 was isolated from surface-sterilized roots of Beta vulgaris. Its effect on sugar beet seedling growth was studied using pot assays and field experiments. This strain promoted beet seedling growth following seed inoculation by seed dipping. Plant height and dry weight of beet increased by 19% and 69%, respectively, compared with controls. Strain 3-1 exhibited the ability to increase absorption of N, P, K, and Mg elements from soil and increase the content of vitamins B and C, and protein within beet. In addition, the strain also produced a phytohormone-auxin, produced nearly twice as much IAA as that produced by strain 2-2, and was able to solubilize phosphates. The concentration of dissolved P in the medium was 180.5 mg L -1 after 4 days of incubation. In field experiments, strain 3-1 significantly increased the content of sucrose, fructose, and the yield of the beet. The growth-promoting properties of Acinetobacter johnsonii strain 3-1 indicates that this promising isolate merits further investigation into its symbiosis with beet plants and its potential application in agriculture. © 2011 Springer Science+Business Media B.V.


Shi Y.,Xinjiang Academy of Agriculture Science | Shi Y.,Shihezi University | Lou K.,Xinjiang Academy of Agriculture Science | Li C.,Beijing Institute of Technology
Photosynthesis Research | Year: 2010

Very little is known about the physiological interactions between plants and endophytic bacteria. We investigated the impact of three endophytic bacteria, Bacillus pumilus 2-1, Chryseobacterium indologene 2-2, and Acinetobacter johnsonii 3-1, on the photosynthetic capacity and growth of sugar beet. Endophyte-free plants were obtained first and infected with the bacteria. Measurements of total chlorophyll content revealed very significant differences between endophyte-free beet plants and some infected by endophytic bacteria. The maximum photochemical yield (Fv/Fm) was used to determine any photosynthetic effect on plants caused by biotic or abiotic factors. After 30 days of growth, there was significantly higher Fv/Fm for endophyte-infected than endophyte-free plants. The light response curves of beet showed that photosynthetic capacity was significantly increased in endophyte-infected plants. Photosynthesis of endophyte-free plants was saturated at 1,300 μmol m-2 s-1, whereas endophyte-infected plants were not saturated at the irradiance used. The effect seemed to be due to promotion of electron transport in the thylakoid membranes. Promotion of photosynthetic capacity in sugar beet was due to increased chlorophyll content, leading to a consequent increased carbohydrate synthesis. It is possible that the increased maximum yield of photosynthesis in sugar beet was promoted by phytohormones and produced by the bacteria. © 2010 Springer Science+Business Media B.V.


Zhang T.,Xingjian Laboratory of Special Environmental Microbiology | Xu J.,Xingjian Laboratory of Special Environmental Microbiology | Zeng J.,Xingjian Laboratory of Special Environmental Microbiology | Lou K.,Xinjiang Academy of Agriculture Science
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2013

Bacterial and archaeal diversity in surface soils of three coal-fire vents was investigated by T-RFLP analysis and clone libraries of 16S rRNA genes. Soil analysis showed that underground coal fires significantly influenced soil pH, moisture and NO3 - content but had little effect on other elements, organic matter and available nutrients. Hierarchical cluster analysis showed that bacterial community patterns in the soils were very similar, but abundance varied with geographic distance. A clone library from one soil showed that the bacterial community was mainly composed of Firmicutes, Proteobacteria, Acidobacteria, Bacteroidetes, Planctomycetes, Actinobacteria, and unidentified groups. Of these, Firmicutes was the most abundant, accounting for 71.4 % of the clones, and was mainly represented by the genera Bacillus and Paenibacillus. Archaeal phylotypes were closely related to uncultivated species of the phyla Crenarchaeota (97.9 % of clones) and Thaumarchaeota (2.1 %). About 28 % of archaeal phylotypes were associated with ammonia oxidization, especially phylotypes that were highly related to a novel, ammonia-oxidizing isolate from the phylum Thaumarchaeota. These results suggested that microbial communities in the soils were diverse and might contain a large number of novel cultivable species with the potential to assimilate materials by heterotrophic metabolism at high temperature. © 2012 Springer Science+Business Media B.V.


Li H.,Xinjiang Academy of Agriculture Science
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2011

In order to investigated composition and diversity of bacterial in a cold sulfur spring in Xinjiang faulting zone. Environmental total DNA was directly extracted from the water of the No. 10 cold sulfur spring. The 16S rRNA genes were amplified from the total DNA by PCR with bacteria-specific primers and construction a clone library. Positive clones were randomly selected from the library and identified by restriction fragment length polymorphism (RFLP). The unique RFLP pattern corresponded sequences were sequenced, BLAST and then constructed phylogenetic tree. In total, 228 positive clones were screened and grouped into 33 Operational Taxonomic Units (OTUs). The clone coverage C value was 92%. 33 Operational Taxonomic Units were divided into 3 phyla with Blast analysis and RDP classifer: Proteobacteria, Bacteroidetes, and Firmicutes. Proteobacteria (98%) was the absolutely dominant group, of which 20% of the clones were highly related to the known photoautotrophic and chemoautotrophic bacteria ( > 97% sequence similarity). Besides, 64% of the clones showed less than 96% of sequence similarity with sequence deposited in GenBank database, of them 54% sequences were affiliated to genus Legionella spp. Bacterial diversity in No. 10 cold sulfur spring was low, but maybe have a diversity of novel species and lineages. In addition, large number of novel species of Legionella were detected in the spring water may suggest the water potentially a source of Legionnaires disease and may constitute a menace to the health of human and livestock that lived down the spring.


PubMed | Xinjiang Academy of Agriculture Science
Type: Journal Article | Journal: Huan jing ke xue= Huanjing kexue | Year: 2012

The aim was to compare the characteristics and the differences in carbon catabolic diversity of air samples collected from five locations that around the edge of Taklamakan desert. The characteristics and the differences of carbon metabolic profiles were detected by using the BIOLOG micro plate (BIOLOG EcoPlate). The results showed that the average well color development (AWCD) curve of all five samples did not reach clear saturation during the incubation time (10 days), but differences among them were significant. The highest AWCD value appeared in Shache and the lowest was in Hotan, which were 0.24 and 0.1, respectively. Carbon utilization showed that all samples exhibited high level of polymer, carbohydrates, amino acids and carboxylic acid; however, amine and the phenol compound were the lowest. Principal components analysis (PCA) indicated that twenty categories of carbon significantly related to PC1 and twelve categories for PC2. Hierarchical cluster analysis showed these five areas could be divided into 2 clusters: (1) Hotan, Pishan, (2) Shache, Luntai, Ulugqat. Canonical correspondence analysis (CCA) showed that those community functional diversities were highly affected by some environmental factors, such as wind speed, altitude, humidity. Further investigation by correlation analysis revealed that the microbial communities using single carbon source were significantly affected by abiotic factors, such as the utilization of beta-methyl-D-glucoside, D-galacturonic acid and putrescine had significantly positive correlation (P < 0.05) with latitude; 2-hydroxy benzoic acid and alpha-D-lactose significantly related to wind speed (P < 0.05); and D-glucosaminic acid was positive with air pressure, but it negatively correlated with altitude (P < 0.05). In conclusion,the carbon sources provided by BIOLOG EcoPlate were utilized slowly by air microbial communities; and the characteristics of the air community carbon catabolic along the edge of the Taklamakan desert revealed regional feature, which may be affected by environmental factors.


PubMed | Anglia, Xinjiang Academy of Agriculture science and Coventry University
Type: Journal Article | Journal: Environmental microbiology | Year: 2016

The volatile organosulfur compound, dimethylsulfide (DMS), plays an important role in climate regulation and global sulfur biogeochemical cycles. Microbial oxidation of DMS to dimethylsulfoxide (DMSO) represents a major sink of DMS in surface seawater, yet the underlying molecular mechanisms and key microbial taxa involved are not known. Here, we reveal that Ruegeria pomeroyi, a model marine heterotrophic bacterium, can oxidize DMS to DMSO using trimethylamine monooxygenase (Tmm). Purified Tmm oxidizes DMS to DMSO at a 1:1 ratio. Mutagenesis of the tmm gene in R. pomeroyi completely abolished DMS oxidation and subsequent DMSO formation. Expression of Tmm and DMS oxidation in R. pomeroyi is methylamine-dependent and regulated at the post-transcriptional level. Considering that Tmm is present in approximately 20% of bacterial cells inhabiting marine surface waters, particularly the marine Roseobacter clade and the SAR11 clade, our observations contribute to a mechanistic understanding of biological DMSO production in surface seawater.


Zeng J.,Xinjiang Academy of Agriculture Science | Zeng J.,CAS Research Center for Eco Environmental Sciences | Deng L.-J.,Xinjiang Academy of Agriculture Science | Lou K.,Xinjiang Academy of Agriculture Science | And 4 more authors.
Journal of Basic Microbiology | Year: 2014

The prokaryotic diversity in two brackish lakes (Sayram Lake and Chaiwopu Lake) was investigated by constructing bacterial and archaeal clone libraries of 16S rRNA genes. Bacterial clones from Sayram Lake were classified into six phyla (Proteobacteria, Verrucomicrobia, Bacteroidetes, Planctomycetes, Acidobacteria, Actinobacteria). Of these, Proteobacteria and Verrucomicrobia were the most dominant, representing 50.4 and 16.8% of the clone library, respectively. Sequences related to Proteobacteria (58.1%), Cyanobacteria (17.2%), Bacteroidetes (15%), Verrucomicrobia (4.3%), Actinobacteria (3.2%) constituted over 97% of the bacterial clone library from Chaiwopu Lake. In addition, 58.8% (Sayram Lake) and 48% (Chaiwopu Lake) of bacterial clones showed high sequence identity to pure cultures. The composition of Archaea was obviously different between the two lakes. Only the Crenarchaeota phylum was found in the Sayram Lake, whereas Archaeal sequences from Chaiwopu Lake were classified into three phyla: Crenarchaeota (5.8%), Thaumarchaeota (81.2%), and Euryarchaeota (13%). Among the archaeal sequences, 94.2% were highly related to cultivable species of the genus Nitrosopumilus, Methanoculleus, and Methanobacterium. These results showed a high diversity of potential cultivable heterotrophic bacteria in Sayram Lake and Chaiwopu Lake. Chaiwopu Lake was a source of potentially novel, cultivable archaea. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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