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Zhang T.,Xingjian Laboratory of Special Environmental Microbiology | Xu J.,Xingjian Laboratory of Special Environmental Microbiology | Zeng J.,Xingjian Laboratory of Special Environmental Microbiology | Lou K.,Xinjiang Academy of Agriculture Science
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2013

Bacterial and archaeal diversity in surface soils of three coal-fire vents was investigated by T-RFLP analysis and clone libraries of 16S rRNA genes. Soil analysis showed that underground coal fires significantly influenced soil pH, moisture and NO3 - content but had little effect on other elements, organic matter and available nutrients. Hierarchical cluster analysis showed that bacterial community patterns in the soils were very similar, but abundance varied with geographic distance. A clone library from one soil showed that the bacterial community was mainly composed of Firmicutes, Proteobacteria, Acidobacteria, Bacteroidetes, Planctomycetes, Actinobacteria, and unidentified groups. Of these, Firmicutes was the most abundant, accounting for 71.4 % of the clones, and was mainly represented by the genera Bacillus and Paenibacillus. Archaeal phylotypes were closely related to uncultivated species of the phyla Crenarchaeota (97.9 % of clones) and Thaumarchaeota (2.1 %). About 28 % of archaeal phylotypes were associated with ammonia oxidization, especially phylotypes that were highly related to a novel, ammonia-oxidizing isolate from the phylum Thaumarchaeota. These results suggested that microbial communities in the soils were diverse and might contain a large number of novel cultivable species with the potential to assimilate materials by heterotrophic metabolism at high temperature. © 2012 Springer Science+Business Media B.V. Source


Li H.,Xinjiang Academy of Agriculture Science
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2011

In order to investigated composition and diversity of bacterial in a cold sulfur spring in Xinjiang faulting zone. Environmental total DNA was directly extracted from the water of the No. 10 cold sulfur spring. The 16S rRNA genes were amplified from the total DNA by PCR with bacteria-specific primers and construction a clone library. Positive clones were randomly selected from the library and identified by restriction fragment length polymorphism (RFLP). The unique RFLP pattern corresponded sequences were sequenced, BLAST and then constructed phylogenetic tree. In total, 228 positive clones were screened and grouped into 33 Operational Taxonomic Units (OTUs). The clone coverage C value was 92%. 33 Operational Taxonomic Units were divided into 3 phyla with Blast analysis and RDP classifer: Proteobacteria, Bacteroidetes, and Firmicutes. Proteobacteria (98%) was the absolutely dominant group, of which 20% of the clones were highly related to the known photoautotrophic and chemoautotrophic bacteria ( > 97% sequence similarity). Besides, 64% of the clones showed less than 96% of sequence similarity with sequence deposited in GenBank database, of them 54% sequences were affiliated to genus Legionella spp. Bacterial diversity in No. 10 cold sulfur spring was low, but maybe have a diversity of novel species and lineages. In addition, large number of novel species of Legionella were detected in the spring water may suggest the water potentially a source of Legionnaires disease and may constitute a menace to the health of human and livestock that lived down the spring. Source


Shi Y.,Xinjiang Academy of Agriculture Science | Shi Y.,Shihezi University | Lou K.,Xinjiang Academy of Agriculture Science | Li C.,Beijing Institute of Technology
Symbiosis | Year: 2011

An endophytic bacteriumn identified as Acinetobacter johnsonii strain 3-1 was isolated from surface-sterilized roots of Beta vulgaris. Its effect on sugar beet seedling growth was studied using pot assays and field experiments. This strain promoted beet seedling growth following seed inoculation by seed dipping. Plant height and dry weight of beet increased by 19% and 69%, respectively, compared with controls. Strain 3-1 exhibited the ability to increase absorption of N, P, K, and Mg elements from soil and increase the content of vitamins B and C, and protein within beet. In addition, the strain also produced a phytohormone-auxin, produced nearly twice as much IAA as that produced by strain 2-2, and was able to solubilize phosphates. The concentration of dissolved P in the medium was 180.5 mg L -1 after 4 days of incubation. In field experiments, strain 3-1 significantly increased the content of sucrose, fructose, and the yield of the beet. The growth-promoting properties of Acinetobacter johnsonii strain 3-1 indicates that this promising isolate merits further investigation into its symbiosis with beet plants and its potential application in agriculture. © 2011 Springer Science+Business Media B.V. Source


Gao Y.,Shihezi University | Gao Y.,Xinjiang Academy of Agriculture Science | Lou K.,Xinjiang Academy of Agriculture Science | Li C.,Shihezi University
Nongye Gongcheng Xuebao/Transactions of the Chinese Society of Agricultural Engineering | Year: 2011

The effects of exogenous glycine betaine on the physiological responses of cotton seedings under salt stress were studied. No.18 cotton widely cultivated in Xinjiang was selected as study object under 200 mmol/L NaCL stress and spraying 5 mmol/L, 10 mmol/L glycine betaine respectively. The changes of physiological index were measured after 7 day. The results showed that under the condition of no salt stress, the content of proline and soluble sugar were improved significantly, while malonaldehyde (MDA) and antioxidative enzyme were not affected by spraying glycine betaine.The content of MDA increased significantly and proline, soluble sugar and antioxidative enzymes activity increased under salt stress. After spraying glycine betaine under salt stress, the production of MDA were inhibitted effectually, at the same time the content of proline, soluble sugar and antioxidative enzyme increased further more. Glycine betaine alleviated the damage of cotton seedings caused by salt tress, and 5 mmol/L glycine betaine had better effect. Source


Liu X.,Shandong University | Zhao Y.,Shandong University | Sun B.,Xinjiang Academy of Agriculture Science | Liu C.,Xinjiang Academy of Agriculture Science | And 2 more authors.
Plant, Cell and Environment | Year: 2013

In this study, the ZmPIS gene with a maize ubiquitin promoter was introduced into maize (Zea mays L.) inbred line DH4866 by an Agrobacterium-mediated method to explore the function of the ZmPIS gene in the response of maize to drought stress. The overexpression of ZmPIS in maize resulted in significantly elevated levels of most phospholipids, galactolipids in leaves compared with those in WT and markedly up-regulated expression of some genes involved in the phospholipids metabolism pathway and the ABA biosynthesis pathway after drought stress. Consistent with these results, the drought stress tolerance of the ZmPIS sense transgenic plants were enhanced significantly compared to WT maize plants. These results imply that ZmPIS regulate the plant response to drought stress through altering membrane lipid composition and increasing ABA synthesis in maize. Phosphatidylinositol (PtdIns) synthase is a key enzyme in the phospholipid pathway and catalyses the formation of PtdIns. PtdIns is not only a structural component of cell membranes, but also the precursor of the phospholipid signal molecules that regulate plant response to environment stresses. Here, we obtained transgenic maize constitutively overexpressing or underexpressing PIS from maize (ZmPIS) under the control of a maize ubiquitin promoter. Transgenic plants were confirmed by PCR, Southern blotting analysis and real-time RT-PCR assay. The electrospray ionization tandem mass spectrometry (ESI-MS/MS)-based lipid profiling analysis showed that, under drought stress conditions, the overexpression of ZmPIS in maize resulted in significantly elevated levels of most phospholipids and galactolipids in leaves compared with those in wild type (WT). At the same time, the expression of some genes involved in the phospholipid metabolism pathway and the abscisic acid (ABA) biosynthesis pathway including ZmPLC, ZmPLD, ZmDGK1, ZmDGK3, ZmPIP5K9, ZmABA1, ZmNCED, ZmAAO1, ZmAAO2 and ZmSCA1 was markedly up-regulated in the overexpression lines after drought stress. Consistent with these results, the drought stress tolerance of the ZmPIS sense transgenic plants was enhanced significantly at the pre-flowering stages compared with WT maize plants. These results imply that ZmPIS regulates the plant response to drought stress through altering membrane lipid composition and increasing ABA synthesis in maize. © 2012 Blackwell Publishing Ltd. Source

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