Xinjiang Academy of Agriculture and Reclamation Science

Shihezi, China

Xinjiang Academy of Agriculture and Reclamation Science

Shihezi, China
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Ji C.,China Agricultural University | Zhang J.,Xinjiang Academy of Agriculture and Reclamation Science | Yuan T.,China Agricultural University | Li W.,China Agricultural University
Applied Mechanics and Materials | Year: 2014

In order to improve the automated vegetable-harvesting level, a robot system for truss tomato harvesting and two key technologies of picking-point recognition and end-effector design were proposed. An algorithm used the segmentation feature of the color difference 2r-g-b to recognize the truss tomato fruit and the assistant mark. According to the growth characteristics of the stem of tomato truss, the approximate fitting curve of stem and the contour of assistant mark were extracted to generate the optimal picking-point for location of tomato truss. The hardware structure of an end-effector based on flexible transmission was designed, and the function of cutting and grasping could be realized simultaneously by the end-effector. Experimental results show that the success rate for harvesting truss tomato was 88.6%, and the average execution time for picking a truss tomato was 37.2s. © (2014) Trans Tech Publications, Switzerland.

Sun F.,Xinjiang Academy of Agriculture and Reclamation Science | Sun F.,Supervision and Testing Center Food Quality | Luo X.,Xinjiang Academy of Agriculture and Reclamation Science | Luo X.,Supervision and Testing Center Food Quality | And 5 more authors.
Polymer Chemistry | Year: 2015

Hyperbranched polymers (HBPs) have attracted increasing attention of scientists due to their unique physical and chemical properties, as well as their potential applications in different fields. Many methods for the preparation of HBPs have hitherto been reported. This review focuses primarily on several novel synthesis methods for various HBPs. We present and discuss the advantages and disadvantages as well as recent advances relating to such synthesis methods, namely the slow monomer addition method, click chemistry, and the enzyme-catalyzed polymerization method. Besides, the applications of HBPs in analytical chemistry fields such as sample pretreatment and immunosensors have also been briefly summarized. This journal is © The Royal Society of Chemistry.

Zhang T.,Nanjing Agricultural University | Qian N.,Nanjing Agricultural University | Zhu X.,Nanjing Agricultural University | Chen H.,Xinjiang Academy of Agriculture and Reclamation science | And 3 more authors.
PLoS ONE | Year: 2013

Cotton is the world's leading cash crop, and genetic improvement of fiber yield and quality is the primary objective of cotton breeding program. In this study, we used various approaches to identify QTLs related to fiber yield and quality. Firstly, we constructed a four-way cross (4WC) mapping population with four base core cultivars, Stoneville 2B, Foster 6, Deltapine 15 and Zhongmiansuo No.7 (CRI 7), as parents in Chinese cotton breeding history and identified 83 QTLs for 11 agronomic and fiber quality traits. Secondly, association mapping of agronomical and fiber quality traits was based on 121 simple sequence repeat (SSR) markers using a general linear model (GLM). For this, 81 Gossypium hirsutum L. accessions including the four core parents and their derived cultivars were grown in seven diverse environments. Using these approaches, we successfully identified 180 QTLs significantly associated with agronomic and fiber quality traits. Among them were 66 QTLs that were identified via linkage disequilibrium (LD) and 4WC family-based linkage (FBL) mapping and by previously published family-based linkage (FBL) mapping in modern Chinese cotton cultivars. Twenty eight and 44 consistent QTLs were identified by 4WC and LD mapping, and by FBL and LD mapping methods, respectively. Furthermore, transmission and variation of QTL-alleles mapped by LD association in the three breeding periods revealed that some could be detected in almost all Chinese cotton cultivars, suggesting their stable transmission and some identified only in the four base cultivars and not in the modern cultivars, suggesting they were missed in conventional breeding. These results will be useful to conduct genomics-assisted breeding effectively using these existing and novel QTL alleles to improve yield and fiber qualities in cotton. © 2013 Zhang et al.

PubMed | Xinjiang Academy of Agriculture and Reclamation Science, Key Laboratory of Chicken Genetics and Breeding at Ministry of Agriculture and Northeast Agricultural University
Type: Journal Article | Journal: PloS one | Year: 2015

The Distal-less 3 (homeobox protein DLX-3), a transcription factor, is critical for the development of hair follicle and hair formation and regeneration. We previously identified and found that four SNPs (c. *118T>C, c. *228T>C, c. *688A>G and c. *1,038_1,039 insC) in 3 untranslated region (UTR) of sheep DLX3 were in high linkage disequilibrium with each other and significantly associated with wool crimp (P<0.05), however, the underlying mechanisms by which these SNPs affect the wool crimp remains unknown. In the present study, we performed association analysis between these four identified SNPs and DLX3 gene expression in sheep skin using quantitative real-time RT-PCR. The results showed that these SNPs were significantly associated with sheep skin DLX3 mRNA expression levels. Then, we constructed DLX3 3UTR luciferase reporters and validated the association. The reporter assays showed that the three major haplotypes, derived from the four SNPs, had significantly different effects on luciferase reporter activity and the four SNPs also had significantly different allelic effects on the luciferase reporter activity (p < 0.05). Bioinformatics analysis showed that the SNP (c. *1,038_1,039 insC) was located within a potential miR-188 binding site of the 3UTR of sheep DLX3 mRNA. This SNP may affect miR-188-mediated DLX3 gene expression and result in phenotypic variation. To test the hypothesis, we investigated the effects of miR-188 mimic and inhibitor on the activity of the DLX3 3UTR luciferase reporter with different SNP alleles. The results showed that in both sheep fetal fibroblasts (SFFs) and human HaCaT cells, miR-188 mimic could significantly decrease the allele D (deletion) luciferase reporter activity (p < 0.05), but miR-188 inhibitor could increased the reporter activitiy. However, neither miR-188 mimc nor inhibitor could influence the allele I (insertion) reporter activity. In addition, transfection of miR-188 mimic dramatically decreased the endogenous expression of DLX3 in SFFs (p < 0.05). Taken together, we demonstrated that DLX3 is a target gene of miR-188 and the SNP (c. *1,038_1,039 insC) is a functional SNP, and affects miR-188-mediated gene regulation of sheep DLX3. Our finding may in part explain allelic difference in gene expression and wool crimp in our tested sheep population.

Wang X.,Huazhong Agricultural University | Yu Y.,Huazhong Agricultural University | Yu Y.,Xinjiang Academy of Agriculture and Reclamation Science | Sang J.,Huazhong Agricultural University | And 3 more authors.
Australian Journal of Crop Science | Year: 2013

Gossypium barbadense, famous for the superior quality of its fibers, is the second most cultivated cotton in the world. In order to determine the genetic basis of its agronomic traits, a linkage map was constructed and QTLs were analyzed. A total of 15,971 markers, including gSSRs, EST-SSRs, SRAPs, and SSCP-SNPs, were used to construct an intraspecific linkage map of G barbadense with 124 F2 individuals derived from the cross (Hai7124 × 3-79). In the F2 population, 412 loci showed polymorphism, giving a polymorphic rate of 2.58%. Three hundred and thirty-seven loci were mapped on 52 linkage groups, and 35 groups were assigned to 20 chromosomes. The full length of the linkage map was 2108.34 cM, and the mean distance between adjacent loci was 6.26 cM. Fifty-two loci (12.62%) showed segregation distortion (P

Wu X.-D.,Yangtze University | Liu W.,Xinjiang Academy of Agriculture and Reclamation Science
Applied Mechanics and Materials | Year: 2012

Hydraulic disc brake is essential for meeting the requirements of safety and economic efficiency during drilling and work-over procedure. To address this need, we have developed a programmable logic controller controlled novel hydraulic disc brake system with capability to apply control strategy similar to anti-lock braking system for hoisting system of truck-mounted drilling rig. This paper is to investigate the complicated interaction between the work clamp and the hydraulic control system. So designers can use this model to decide which control strategies are more suitable for the disc brake. This is demonstrated by modeling the advanced hydraulic system and simulating influential factors on dynamic characteristics of hoisting system disc brake, with the application of the Advanced Modeling Environment for Simulations of Engineering Systems. The method of modeling and simulation provides a simple, effective approach to studying hydraulic disc brake for hoisting system.

Yu Y.,Huazhong Agricultural University | Yu Y.,Xinjiang Academy of Agriculture and Reclamation Science | Yuan D.,Huazhong Agricultural University | Liang S.,Huazhong Agricultural University | And 4 more authors.
BMC Genomics | Year: 2011

Background: Cotton, with a large genome, is an important crop throughout the world. A high-density genetic linkage map is the prerequisite for cotton genetics and breeding. A genetic map based on simple polymerase chain reaction markers will be efficient for marker-assisted breeding in cotton, and markers from transcribed sequences have more chance to target genes related to traits. To construct a genome-wide, functional marker-based genetic linkage map in cotton, we isolated and mapped expressed sequence tag-simple sequence repeats (EST-SSRs) from cotton ESTs derived from the A1, D5, (AD)1, and (AD)2genome.Results: A total of 3177 new EST-SSRs developed in our laboratory and other newly released SSRs were used to enrich our interspecific BC1genetic linkage map. A total of 547 loci and 911 loci were obtained from our EST-SSRs and the newly released SSRs, respectively. The 1458 loci together with our previously published data were used to construct an updated genetic linkage map. The final map included 2316 loci on the 26 cotton chromosomes, 4418.9 cM in total length and 1.91 cM in average distance between adjacent markers. To our knowledge, this map is one of the three most dense linkage maps in cotton. Twenty-one segregation distortion regions (SDRs) were found in this map; three segregation distorted chromosomes, Chr02, Chr16, and Chr18, were identified with 99.9% of distorted markers segregating toward the heterozygous allele. Functional analysis of SSR sequences showed that 1633 loci of this map (70.6%) were transcribed loci and 1332 loci (57.5%) were translated loci.Conclusions: This map lays groundwork for further genetic analyses of important quantitative traits, marker-assisted selection, and genome organization architecture in cotton as well as for comparative genomics between cotton and other species. The segregation distorted chromosomes can be a guide to identify segregation distortion loci in cotton. The annotation of SSR sequences identified frequent and rare gene ontology items on each chromosome, which is helpful to discover functions of cotton chromosomes. © 2011 Yu et al; licensee BioMed Central Ltd.

PubMed | Xinjiang Academy of Agriculture and Reclamation Science
Type: Journal Article | Journal: Journal of separation science | Year: 2016

Monodisperse molecularly imprinted polymers for oleanolic acid were successfully prepared by a precipitation polymerization method using oleanolic acid as a template, methacrylic acid as a functional monomer, and divinylbenzene/ethylene glycol dimethacrylate as a crosslinker in a mixture of acetonitrile and ethanol (3:1, v/v). The imprinted polymers and nonimprinted polymers were characterized by using scanning electron microscopy, Fourier transform infrared spectroscopy, and thermogravimetric analysis. The resulting imprinted polymers had average diameters of 3.15 m and monodispersity values of 1.024. The results clearly demonstrate that use of ethanol as a cosolvent is indeed exceedingly effective in promoting the dissolution of oleanolic acid and in obtaining uniform microspheres. Molecular recognition properties and binding capability to oleanolic acid were evaluated by adsorption testing, which indicated that the imprinted polymers displayed optimal binding performance with a maximum adsorption capacity of 17.3 mg/g and a binding saturation time of 80 min. Meanwhile, the produced imprinted polymers exhibited higher selectivity to oleanolic acid than that for ursolic acid and rhein. Herein, the studies can provide theoretical and experimental references for the oleanolic acid molecular imprinted system.

PubMed | Huazhong Agricultural University and Xinjiang Academy of Agriculture and Reclamation Science
Type: Journal Article | Journal: PloS one | Year: 2015

To provide a resource for cotton genetics and breeding, an interspecific hybridization between Gossypium hirsutum cv. Emian22 and G. barbadense acc. 3-79 was made. A population of 54 BILs (backcross inbred lines, BC1F8) was developed with the aim of transferring G. barbadense genes into G. hirsutum in order to genetically analyze these genes function in a G. hirsutum background and create new germplasms for breeding. Preliminary investigation of the morphological traits showed that the BILs had diverse variations in plant architecture, seed size, and fuzz color; the related traits of yield and fiber quality evaluated in 4 environments also showed abundant phenotypic variation. In order to explore the molecular diversity of the BIL population, 446 SSR markers selected at an average genetic distance of 10 cM from our interspecific linkage map were used to genotype the BIL population. A total of 393 polymorphic loci accounting for 84.4% MAF (major allele frequency) > 0.05 and 922 allele loci were detected, and the Shannon diversity index (I) was 0.417 per locus. The average introgression segment length was 16.24 cM, and an average of 29.53 segments were introgressed in each BIL line with an average background recovery of 79.8%. QTL mapping revealed 58 QTL associated with fiber quality and yield traits, and 47 favored alleles derived from the donor parent were discovered. This study demonstrated that the interspecific BIL population was enriched with much phenotypic and molecular variation which could be a resource for cotton genetics and breeding.

PubMed | Xinjiang Academy of Agriculture and Reclamation Science
Type: Journal Article | Journal: Analytical and bioanalytical chemistry | Year: 2015

This study presents a novel analytical method for the detection of oxytetracycline (OTC) in complex food matrices based on a direct competitive enzyme-linked aptamer assay and magnetic separation technology. In this protocol, free OTC competed with horseradish peroxidase labeled OTC (OTC-HRP) for binding to the OTC aptamer immobilized on magnetic beads. The parameters that can affect the response, such as avidin concentration, aptamer concentration, OTC-HRP concentration, incubation temperature, incubation time, blocking agent, and binding buffer, were optimized. Under the optimal conditions, the linear range for the OTC concentration detection is 0.5-100 ng mL(-1), with a concentration of OTC needed to obtain 50 % of the maximum signal of 14.47 ng mL(-1). The limit of detection and the limit of quantitation were 0.88 and 3.40 ng mL(-1), respectively. There was no obvious cross-reactivity with most of the tetracycline pesticides. The recovery rates ranged from 71.0 to 91.2 % for the food samples, including chicken, milk, and honey, and the relative standard deviation was less than 15.0 %. The proposed method was applied to measure OTC in real samples, and was validated using high-performance liquid chromatography. This method has the advantages of magnetic separation and the concentration effect of magnetic nanoparticles, the specificity of the aptamer, and the high-throughput of microtiter plates; it offers a promising approach for the screening of OTC because it is simple, rapid, highly sensitive, and has low cost.

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