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Wang Z.R.,Xinjiang Academy of Agricultural and Reclamation Science
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases | Year: 2012

A SYBR green real-time fluorescent quantitative PCR assay was developed for detection of Moniezia expansa mRNA with its beta-tubulin as an internal control. The results showed a good linear relationship (>0.99) between the Ct value and the concentration of positive plasmid for each gene from scolex and various proglottids. Real-time PCR showed that the expression abundance of translation elongation factor and primase was different. In conclusion, the transcription level of translation elongation factor and primase was high in both scolex and immature segment, suggesting that they may play a role in the development of scolex and immature segment. Source


Xu Q.,Xinjiang Academy of Agricultural and Reclamation Science
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2012

To analyze the diversity of bacterial community in rectum of diarrheic calves, and differences with health calves. 16S rRNA clone libraries were constructed, positive clones were digested by Msp I and Hha I for restriction fragment length polymorphism (RFLP), and then a phylogenetic tree was depicted based on the 16S rRNA sequencing, to confirm the compose of microbe in the diarrheic calf rectum. The positive rate of clone was 98.75% (474/480) in diarrheic calves, the dominant bacteria included Lactobacillus (14%), Enterococcus (10%) and Escherichia (8%). The positive rate of clone was 96.45% (488/506) in health samples, the dominant bacteria included Clostridium (13%), Bifidobacterium (8%), Megasphaera (5%). Complexity and diversity of bacterial community in rectum in 2 weeks old calves had their own features, and significant increase of Lactobacillus, Enterococcus and Escherichia was found in diarrhea calves. Source


Gao L.,Xinjiang Academy of Agricultural and Reclamation Science
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji | Year: 2013

Studies have shown that clock gene Cry1 may have important roles in the endocrine process of seasonal reproduction in mammals. In this study, Duolang sheep (non-seasonal reproduction sheep breed) and Chinese Merino (seasonal reproduction sheep breed) were used to determine the expression change of Cry1 in hypothalamus-pituitary-ovary axis in different stage of estrous cycle by quantitative real-time PCR. The results showed that the Cry1 mRNA was expressed in all tested tissues, in which the expression levels in pineal gland and thyroid gland were higher than in other tissues. As far as different sheep breeds were concerned, the tissue expression profiles of Cry1 at different stage of estrous cycle were broadly similar. Besides hypothalamus, the expression levels of Cry1 in ovary, uterus, pineal gland, pituitary gland, and thyroid gland were all reached to peak in proestrus. The differences of expression change extent for Cry1 in vary, uterus, pineal gland, and pituitary gland in proestrus and oestrus were significant between different sheep breeds. The results suggested that Cry1 may play roles in switching on the estrus and seasonal reproduction. Source


Chen X.,Xinjiang Academy of Agricultural and Reclamation Science | Zhong L.,Shihezi University
Nongye Gongcheng Xuebao/Transactions of the Chinese Society of Agricultural Engineering | Year: 2012

According to the weakness of the high seed dropping position, the big variability of seed-spaces in soil and the low qualification rate of the double driving discs furrow opener of no-tillage planter, a new belt type seed delivery device was designed and developed. Based on the analysis of the cavitation time of suction seed metering device, the new belt-type seed device combined transmission and dropping seed institutions, which was related with the speed of seed metering device and the speed of planter. Also its main structural parameters were determined. Comprehensive impact factors affected the three test indicators differently, the primary and secondary order of influencing factors for seeding quality was determined, and the results showed that the order was the height of the throwing point, speed of the take seed plate and the suction pressure. The optimum parameter combination was that the height of the throwing point was 100 mm, the speed of the take seed plate was 30 r/min and the suction pressure was 3.5 kPa. At these conditions, particles away from a pass rate were 98.50%, the leakage sowing rate was 0.48% and replay rate was 1.02%. Through experimental verification, the error of experimental and theoretical results was 0.11%, 0.17% and 0.08%, which met the needs of the test requirements. The research provides a reference for further improving quality and optimizing the parameter for air-suction metering device. Source


Zhiyuan N.,Nanjing Agricultural University | Chen H.,Xinjiang Academy of Agricultural and Reclamation Science | Mei H.,Nanjing Agricultural University | Zhang T.,Nanjing Agricultural University
Euphytica | Year: 2014

Cotton is a high-value per acre crop that is produced as a raw material for the textile industry. With the development of new technologies in the textile industry, much attention has been paid to fiber quality in conjunction with yield. The introgression cultivar "Acala Prema" is extensively planted in the Carolina/USA for its good fiber qualities, high yields and tolerance to Verticillium wilt. To conduct QTL mapping for fiber quality and yield in Acala-Prema, we developed a population of 180 recombinant inbred lines (RILs) from a single seed derived from a cross between this line and Chinese cultivar 86-1. We examined the yield performance of the RILs in five Chinese environments and fiber qualities in seven Chinese environments. A genetic linkage map comprising 279 loci was constructed using this RIL population, chiefly with SSR markers, and QTLs were repeatedly identified across diverse environments using the composite interval mapping method. A total of 86 nonredundant QTLs for yield and its components and fiber qualities were independently detected in five or seven environments; Prema alleles were responsible for the increase in trait values for 46 QTLs, while 86-1 was responsible for 40 QTLs. Notably, we detected the stable fiber strength QTL qFS-D3-1, which explained 4.51-17.55 % of PV, with LOD scores ranging from 2.83 to 7.09, and the fiber length qFL-D11-1, which explained 10.02-25.34 % of the PV. Eighteen environment epistatic QTLs were also detected. The QTLs detected in this study provide new information for improving fiber quality and may be especially valuable for marker-assisted selection. © 2013 Springer Science+Business Media Dordrecht. Source

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