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Chen X.,China University of Technology | Huang F.,China Tobacco Zhejiang Industrial Co. | Huang W.,Hubei Institute for Food and Drug Control | We H.,Xinhua Hospital of Hubei Province | And 5 more authors.
Journal of Chemical and Pharmaceutical Research

Pu'er tea served as main ingredient in conjunction with extracts of chrysanthemum, bamboo leaves and tremella was developed to be pie-shaped compound Pu'er tea, compound Pu'er tea bags and instant compound Pu'er tea. The sensory evaluation of the compound Pu'er tea and its feedstock were carried out,and their quality and texture chemicals such as tea polyphenols, free amino acid, caffeine water-soluble saccharides, crude fiber and total ash was assayed. The hydroxyl radical scavenging rates of the compound pu'er tea reached levels of 47-70%.The splenocyte treated with instant compound Pu'er tea had significantly lower splenocyte proliferative responding to ConA (P < 0.01), which did not yet perform in a dose-dependent manner. Source

Gao S.,Woman and Child Hospital of Hubei Province | Li H.,Woman and Child Hospital of Hubei Province | Zhou X.-Q.,Woman and Child Hospital of Hubei Province | You J.-B.,Woman and Child Hospital of Hubei Province | And 4 more authors.
Cellular and Molecular Biology

Withaferin A (WFA) is an active compound from Withania somnifera and has been reported to exhibit a variety of pharmacological activities such as anti-inflammatory, immunomodulatory and anti-tumor properties. In the present study, we investigated the potential protective role of WFA on acute lung injury in neonatal rats induced by lipopolysaccharide (LPS). We found that WFA significantly attenuated the pathological changes of lungs induced by LPS injection. Administration with WFA obviously decreased pulmonary neutrophil infiltration accompanied with decreased MPO concentrations. WFA also reduced the expression of pro-inflammatory cytokines including MIP-2, TNF-α, IL-1β and IL-6. Meanwhile, the expression levels of anti-inflammatory mediators such as TGF-β1 and IL-10 were significantly increased following WFA administration. Moreover, WFA protected LPS-treated rats from oxidative damage via up-regulation of TBARS and H2O2 concentrations and down-regulation of ROS contents. Taken together, the present study demonstrated that WFA administration attenuated LPS-induced lung injury through inhibition of inflammatory responses and oxidative stress. © 2015. Source

Chen X.,China University of Technology | Chen X.,Zhejiang GongShang University | Fang Y.,China University of Technology | Nishinari K.,China University of Technology | And 4 more authors.
Carbohydrate Polymers

Hot-water extracts were prepared from fresh tea leaves and fractionated by DEAE-cellulose DE-52 column chromatography to yield one unexplored polysaccharide-conjugate fraction TPC-L (tea polysaccharide conjugates). Chemical components, molecular weight and its distribution, water vapor sorption properties, zeta potentials and optical characteristics of TPC-L were investigated. As compared with injured cell group, the two dosages of TPC-L (150 and 300 μg/mL) were discovered to possess remarkably protective effect on human umbilical vein endothelial cells against impairments induced by high glucose in a dose-dependent manner (p < 0.05, p < 0.001, respectively). Compared with group NC (normal control), the ingestion of 40 mg/kg of TPC-L could significantly reduce blood glucose levels of normal mice ingesting starch, and significant difference of AUC (area under the curve of blood glucose) and ΔAUC (p < 0.05, p < 0.01) at the postprandial time point of 0.5 and 1.0 h were observed. The three dosages of TPC-L (10, 40 and 160 mg/kg) did not significantly lower postprandial blood glucose levels of normal mice ingesting glucose. TPC-L could improve starch tolerance to prevent impaired glucose tolerance (IGT) from developing into diabetes as well as protective effects on HUVE cells against impairments induced by high glucose It was suggested that TPC-L improved IGT through its capability of inhibition on digestive enzymes. © 2014 Elsevier Ltd. Source

Cao F.,Huazhong University of Science and Technology | Luo F.,Xinhua Hospital of Hubei Province | Chen L.,Huazhong University of Science and Technology | Chen H.,Huazhong University of Science and Technology | And 4 more authors.
Journal of Huazhong University of Science and Technology - Medical Science

In order to explore the role of acetylcholine in the pathogenesis of Parkinson's disease (PD), the changes in the concentration of acetylcholine (Ach) in the striatum, the apoptosis of substantia nigra cells, the ultrastructure and the changes of Nissl cells in rats during the morbidity of PD, and the corresponding behaviors in rats with PD were observed. Rat PD model was established by using the modified Thomas method. Eighty-one rats were randomly divided into normal control, sham operation and PD groups and their behavior features were observed at post-operative day (POD) 7, 14 and 21 as three subgroups (n=9 each). The concentration of Ach in the striatum was determined by using high-performance liquid chromatography. The apoptosis of substantia nigra cells was assayed by using TUNEL method. The ultrastructural changes in the substantia nigra were observed under the electron microscopy, and the survival of neurons in the substantia nigra area was examined by using Nissl staining. In PD group at POD 7 to 21, the damage in the substantia nigra area was gradually aggravated, the concentration of Ach, apoptosis rate and turns of rotation were gradually increased, and the number of Nissl cells was gradually reduced over the time as compared with the normal control and sham operation groups (all P<0.05). It was concluded that there exist dynamic changes in Ach concentration, ethology and apoptosis of the substantia nigra cells during the morbidity of PD, suggesting the contribution of apoptosis to the morbidity of PD, and critical role of Ach in the pathogenesis of PD. © Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2012. Source

Tang C.,Fudan University | Sun J.,Xinhua Hospital of Hubei Province | Xue H.,General Hospital of Shenyang Military Command | Yu Y.,General Hospital of Shenyang Military Command | Xu F.,General Hospital of Shenyang Military Command
Turkish Neurosurgery

Aim:Supraorbital keyhole approach provides access to the major part of the anterior circulation aneurysms. Herein, our surgical experience of supraorbital keyhole approach and its some modification have been proposed, Ma terial and Methods: Out of a series of 76 patients harboring 80 aneurysms operated on via a supraorbital keyhole approach with a superciliar or front wrinkle skin incision, there are 70 patients with subarachnoidal bleeding, others are nonruptured aneurysms, Intraoperative rupture occurred in 8 cases, and 4 had multiple aneurysms. Result:There was a good cosmetic results with less approach-related complications .Of 80 aneurysms, 75 aneurysms were clipped successfully by the supraorbital route. Good Glasgow Outcome Scale scores of 4 or 5 were achieved in 95% of the patients at the time of discharge. 2 patients of Grade IV died in the postoperative period due vasospasm. Conclusion:The supraorbital route is recommended for selected anterior circulation aneurysms based on the improved surgical instruments and microsurgical skills. Source

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