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Xingtai, China

Zhang Y.,Peking University | Wang Q.,Peking University | Wang T.,Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine of Hebei Province | Zhang H.,Xingtai Peoples Hospital | And 5 more authors.
International Journal of Biological Macromolecules | Year: 2012

A water-soluble polysaccharide (WATP), with a molecular weight of 6.3×104Da, was isolated from Aster tataricus. According to gas chromatography (GC) analysis, WATP was composed of galactose, glucose, fucose, rhamnose, arabinose and mannose with molar ratios of 2.1:1.3:0.9:0.5:0.3:0.6. The effects of WATP on cell proliferation and apoptosis in human gastric cancer SGC-7901 cells were examined. MTT assay showed that WATP had a perfectly tumor growth inhibitory activity on SGC-7901 cells, but no cytotoxicity on SGC-7901 and primary human polymorphonuclear (PMN) cells analyzed using LDH assay. Flow cytometry analysis indicated that WATP could significantly induce apoptosis of SGC-7901 cells. Furthermore using Rh123 and Fluo-3 as fluorescent probes, respectively, it was found that mitochondrial transmembrane potential (ΔΨm) of treatment groups was significantly lower than that in un-treatment group and the concentration of calcium in cells exposed to WATP for 24h was increased in a dose dependent manner compared with unexposed group. These results suggest that WATP induces apoptosis of SGC-7901 cells through calcium- and ΔΨm-dependent pathways, indicating that it is potentially useful as a natural anti-cancer agent. © 2012 Elsevier B.V. Source


Zhao L.-L.,Xingtai Peoples Hospital
National Medical Journal of China | Year: 2011

Objective: To study the mechanism of sacral plexus injury resulting from zone-II sacral fractures by axial compression. Methods: Six short-term embalmed pelves were obtained with preserving sacral plexus and resected pubic symphysis. A model of zone-II sacral fractures by axial compression was established. Quantitative analysis for fracture displacement was carried out to observe the characteristics and mechanisms of sacral plexus injury. The experimental data were analyzed by SPSS 10.0 statistic software. Results: In the sacral fracture model of axial compression, the sacral plexus nerves of L5 and SI were obviously compressed. The sharp border of fracture segment stabbed the nerves as the distal segment of fractures was displaced to superior-anteriorly. When the displacement exceeded 1 cm, the tension injury of sacral plexus nerves became noticeable, especially at L5, S1 and S2. There was no sacral nerve injury when the distal segment of fractures was displaced posteriorly. Conclusion: The mechanisms of sacral plexus injury are complicated. And it probably have close correlations with stability, orientation, extent and duration of fracture displacement. © 2011 by the Chinese Medical Association. Source


Lu B.,Xingtai Peoples Hospital
World Chinese Journal of Digestology | Year: 2010

AIM: To analyze the X-ray imaging features of esophageal papilloma and polypoid leiomyoma to improve the diagnosis of the two diseases. METHODS: Twenty-three patients with esophageal papilloma and 11 patients with polypoid leiomyoma undergoing double-contrast radiography of the esophagus and pathological examination were analyzed in this study. The X-ray imaging features of the two diseases were summarized. RESULTS: Of 23 cases of esophageal papilloma, 17 (73.9%) showed marginal notches or lobulations, 19 (82.6%) showed a twisted contour line and uneven thickness, 17 (73.9%) showed a granular or map-like appearance of barium meal, 15 (65.2%) had rigid vein walls, and 9 (39.1%) demonstrated a "double-ring" appearance. Of 11 cases of polypoid leiomyoma, 8 (72.7%) had a regular and smooth edge, 9 (81.8%) had a sharp and even contour line, 9 (81.8%) demonstrated a thin wash-like appearance of barium meal, and 8 (72.7%) had soft vein walls. CONCLUSION: Barium meal X-ray examination plays an important role in diagnosing esophageal papilloma and polypoid leiomyoma. Source


Han B.,Xingtai Peoples Hospital
Experimental and Therapeutic Medicine | Year: 2013

The aim of this study was to investigate the mechanism(s) of action of gastrointestinal hormones in the pathogenesis of irritable bowel syndrome (IBS), and the correlation between gastrointestinal hormones and psychological factors. Patients with IBS were divided into IBS with normal emotional state ratings and IBS in anxiety-depressive states groups. The two groups were then subdivided into IBS-constipation predominant (IBS-C) and IBS-diarrhea predominant (IBS-D) groups. Non-IBS patients with normal depression and anxiety ratings were recruited as controls. The serum concentrations of somatostatin (SS) and vasoactive intestinal peptide (VIP) were measured by radioimmunoassay, and the expression of SS and VIP in the colonic mucosa was detected by immunohistochemistry and radioimmunoassay. The anxiety-depression scores of patients with IBS were significantly different from those of the control group (P<0.05). The expression levels of SS and VIP in the serum and colonic mucosa of the patients with IBS were higher compared with those of the control group. Furthermore, the expression level of SS in the IBS-C group demonstrated a significantly larger increase than that in the IBS-D group (P<0.05); however, there was no significant difference in the expression of VIP between the IBS-C and IBS-D groups (P>0.05). In addition, the expression levels of SS and VIP in the IBS groups with normal emotional state ratings were notably different from those in the IBS groups in anxiety-depressive states (P<0.05). Anxiety-depressive states may lead to changes in the secretion of SS and VIP, and subsequently to changes in gastrointestinal motility and function. Source


Gao J.,Xingtai Peoples Hospital
Experimental and Therapeutic Medicine | Year: 2013

The aim of this study was to investigate the correlation between anxiety-depression status and cytokines in diarrhea-predominant irritable bowel syndrome (IBS-D). IBS-D patients were divided into an anxiety-depression IBS-D group and a non-anxiety-depression IBS-D group. Patients without IBS, anxiety or depression were selected as the control group. Scoring was performed using the self-rating anxiety scale (SAS) and self-rating depression scale (SDS). Levels of IL-1β and IL-10 in the blood and sigmoid colon mucosa were detected, and the proportions of IL-1β- and IL-10-positive cells in the sigmoid colon mucosa were determined. The results demonstrated that the SDS and SAS scores in the IBS-D group were significantly higher than those in the control group (P<0.05). The levels of IL-1β in the blood and sigmoid colon mucosa and the proportion of IL-1β-positive cells in the sigmoid colon mucosa in the IBS-D group were significantly higher than those in the control group (P<0.05). The levels of IL-10 in the blood and sigmoid colon mucosa and the proportion of IL-10-positive cells in the IBS-D group were significantly lower than those in the control group (P<0.05). The levels of IL-1β in the blood and sigmoid colon mucosa and the proportion of IL-1β-positive cells in the anxiety-depression IBS-D group were significantly higher than those in the non-anxiety-depression IBS-D group, and the levels of IL-10 and the proportion of IL-10-positive cells in the anxiety-depression IBS-D group were significantly lower than those in the non-anxiety-depression IBS-D group (P<0.05). Anxiety-depression status may cause the IL-1β and IL-10 levels in IBS patients to change and result in an imbalance of the proinflammatory and anti-inflammatory cytokines, leading to the occurrence or aggravation of IBS. Source

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