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Liu G.,Xianshuigu Hospital of Jinnan District | Qi C.,Tianjin Medical University | Xu Q.,Xianshuigu Hospital of Jinnan District | Wu B.,Xianshuigu Hospital of Jinnan District | And 2 more authors.
Tumor Biology | Year: 2014

The methylenetetrahydrofolate dehydrogenase 1 (MTHFD1) polymorphism G1958A has been extensively investigated as a potential risk factor for prostate cancer (PCa), but the results have thus far been inconclusive. This meta-analysis was performed to derive a more precise estimation of the association. A comprehensive search was conducted to identify all case-control studies of MTHFD1 G1958A polymorphism and PCa risk. We used odds ratios (ORs) to assess the strength of the association, and 95 % confidence intervals (CIs) give a sense of the precision of the estimate. Statistical analyses were performed using Review Manage version 5.0 and Stata 10.0. A total of six available studies were considered in the present meta-analysis, with 7,493 patients and 36,941 controls. When all groups were pooled, there was no evidence that G1958A had significant association with PCa under additive, recessive, dominant, and allelic models. This meta-analysis suggests that MTHFD1 G1958A polymorphism might not be a risk factor for PCa. However, further large-scale and well-designed case-control studies are necessary to validate the risk identified in the present meta-analysis. © 2013 International Society of Oncology and BioMarkers (ISOBM).


Huang Y.-H.,Xianshuigu Hospital of Jinnan District
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: Bone marrow mesenchymal stem cells can secrete neurotrophic factors in vitro, and can also be differentiated into neurons, thereby contributing to the repair of traumatic brain injury. However, the short life cycle of bone marrow mesenchymal stem cells influences their protective effects on the damaged brain tissues. OBJECTIVE: To observe the effect of bone marrow mesenchymal stem cell transplantation combined with ganglioside in rats with traumatic brain injury. METHODS: Sixty Wistar rats were used to make severe traumatic brain injury models using a hydraulic head injury instrument, and then randomized into three groups: 1 mL DMEM, 1 mL bone marrow mesenchymal stem cell suspension (1×1010/L), 1 mL bone marrow mesenchymal stem cell suspension (1×1010/L) combined with ganglioside solution (30 mg/kg) were injected respectively in model group, transplantation group and combined group, once a day, totally for 3 days. Neurological behavior scores were observed according to Longa method at 24 hours after modeling and at 3 days, 1, 2, 3, 4 weeks after cell transplantation. At 3 days after cell transplantation, RT-PCR and western blot assay were employed to detect aquaporin 4 mRNA and protein expressions. At 1 week after transplantation, hematoxylin-eosin staining was performed for pathological observation of the damaged brain tissues. RESULTS AND CONCLUSION: At 3 days, 1, 2, 3, 4 weeks after cell transplantation, the neurological behavior scores were ranked as follows: combined group < transplantation group < model group (P < 0.05). At 3 days after cell transplantation, the mRNA and protein expression of aquaporin 4 was ranked as follows: model group > transplantation group > combined group (P < 0.05). Hematoxylin-eosin staining showed that the recovery of brain tissue was better in the combined group than the model and transplantation groups (P < 0.05). These findings indicate that bone marrow mesenchymal stem cells combined with ganglioside can significantly improve the neurological behavior function of rats with traumatic brain injury. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.


Du S.-S.,Xianshuigu Hospital of Jinnan District | Sun S.-S.,Xianshuigu Hospital of Jinnan District | Song Y.-F.,Xianshuigu Hospital of Jinnan District | Hao S.-A.,Xianshuigu Hospital of Jinnan District
World Chinese Journal of Digestology | Year: 2015

AIM: To evaluate the clinical effects of emergency endoscopy in the treatment of acute nonvariceal upper gastrointestinal bleeding. METHODS: A retrospective analysis was performed of 126 patients with acute nonvariceal upper gastrointestinal bleeding who were treated by emergency endoscopy or elective endoscopy at our hospital between March 2010 and September 2013. The causes of acute nonvariceal upper gastrointestinal bleeding were analyzed. The rate of bleeding, the success rate of hemostasis, rebleeding rate, surgery rate and hospitalization time were compared in the two groups. RESULTS: The most common cause of acute non-variceal upper gastrointestinal bleeding was peptic ulcer (44.4%), followed by acute gastric mucosal lesions (12.7%) and gastrointestinal cancer (11.9%). A total of 116 (126) cases of bleeding were detected, and the positive rate was 92.1%. The rate of bleeding was significantly higher in the emergency endoscopy group (97.1%) than in the elective endoscopy group (85.7%) (P < 0.05). The rebleeding rate (2.9%), surgical rate (1.5%) and hospitalization time were significantly lower in the emergency endoscopic group than in the elective endoscopy (P < 0.05). CONCLUSION: Emergency endoscopy is convenient, efficient, and less invasive, and is the preferred treatment for acute non-variceal upper gastrointestinal bleeding. © 2015 Baishideng Publishing Group Inc. All rights reserved.


Fu J.-R.,Xianshuigu Hospital of Jinnan District
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: The amniotic membrane is the rejected material after birth. Amniotic mesenchymal stem cells are characterized as easy harvesting, strong proliferation ability, no ethical controversy, and low immunogenicity. OBJECTIVE: To electrotransfer human telomerase reverse transcriptase (hTERT) gene into amniotic mesenchymal stem cells transplanted into diabetic rats and to explore its effect on diabetic rats. METHODS: Human amniotic mesenchymal stem cells were isolated, cultured and electrotransferred by hTERT gene. Ten of 50 Sprague-Dawley rats were randomized selected as controls, and the remaining rats were used to establish diabetic models through injection of 45 mg/kg streptozotocin. Thirty-six model rats were randomized into model group, cell transplantation group and hTERT-transfected cell transplantation group, with 12 rats in each group. In the latter two groups, human amniotic mesenchymal stem cells and hTERT-transfected amniotic mesenchymal stem cells were injected via sublingual veins, respectively. After transplantation, blood glucose levels were monitored dynamically, and plasma insulin concentration was detected every week. Pancreas tissues were taken and cut into sections for histological observation using hematoxylin-eosin staining. RESULTS AND CONCLUSION: At 4 weeks after transplantation, the blood glucose levels were significantly lower in the two cell transplantation groups than the model group (P < 0.05), and especially in the hTERT-transfected cell transplantation group, the blood glucose level was close to the normal value (P > 0.05). However, the model group still had a higher blood glucose level. At 6 weeks after transplantation, compared with the model group, the plasma insulin concentration was significantly increased in the two cell transplantation group (P < 0.05), and the severity of pancreatic injury was also eased in these two groups (P < 0.05), especially in the hTERT-transfected cell transplantation group (P < 0.05). These findings indicate that hTERT-transfected amniotic mesenchymal stem cell transplantation can dramatically decrease blood glucose level and relieve pancreatic injury in diabetic rats, which is an effective method for treatment of mellitus diabetes in rats. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.


PubMed | Xianshuigu Hospital of Jinnan District
Type: Journal Article | Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine | Year: 2014

The methylenetetrahydrofolate dehydrogenase 1 (MTHFD1) polymorphism G1958A has been extensively investigated as a potential risk factor for prostate cancer (PCa), but the results have thus far been inconclusive. This meta-analysis was performed to derive a more precise estimation of the association. A comprehensive search was conducted to identify all case-control studies of MTHFD1 G1958A polymorphism and PCa risk. We used odds ratios (ORs) to assess the strength of the association, and 95% confidence intervals (CIs) give a sense of the precision of the estimate. Statistical analyses were performed using Review Manage version 5.0 and Stata 10.0. A total of six available studies were considered in the present meta-analysis, with 7,493 patients and 36,941 controls. When all groups were pooled, there was no evidence that G1958A had significant association with PCa under additive, recessive, dominant, and allelic models. This meta-analysis suggests that MTHFD1 G1958A polymorphism might not be a risk factor for PCa. However, further large-scale and well-designed case-control studies are necessary to validate the risk identified in the present meta-analysis.

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