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Liu Q.-S.,Central Hospital of Xianning | Dong W.-G.,Hubei University
World Chinese Journal of Digestology | Year: 2011

AIM: To evaluate common clinical problems of percutaneous endoscopic gastrostomy and create an individualized treatment plan by means of evidence-based medicine to control symptoms and reduce mortality. METHODS: After clinical problems were put forward, systematic reviews and randomized controlled trials were collected from the Cochrane Library (online), MEDLINE (1990-2010), EMBase (1990-2010), SCIE (1990-2010) and CNKI (2000-2010). Treatment protocol was developed by combining the evidence and the wishes of the patient. RESULTS: A total of 39 RCTs and 10 systematic reviews were identified. A rational treatment plan was made upon a serious evaluation of the data and wishes of patients. CONCLUSION: An individualized treatment plan developed based on evidence-based method can improve the efficacy of percutaneous endoscopic gastrostomy.


Liu Q.-S.,Central Hospital of Xianning | Liu Q.-S.,Wuhan University | Dong W.-G.,Wuhan University
Chinese Journal of Evidence-Based Medicine | Year: 2012

Objective To discuss the common clinical problems and make the individualized treatment for a patient with obscure gastrointestinal bleeding by means of evidence-based medicine, so as to ultimately control the symptoms and reduce the mortality. Methods After the clinical problems were put forward, the systematic reviews and randomized controlled trials (RCTs) were collected in The Cochrane Library (online), MEDLINE, EMBase, SCIE and CNKI databases, from the date of their establishment to 2010. The treatment protocol was made by combining the assessment of evidence with the willingness of both patient and relatives. Results A total of 30 RCTs and 5 systematic reviews were identified. A rational diagnostic and therapeutic plan was made upon a serious evaluation of the data and willingness of patients. The Mickel's diverticulum was found through capsule endoscopy, which was then locally excised under laparoscopy. After a 6-month follow-up, the plan proved to be optimal. Conclusion The treatment efficacy in diagnosed obscure gastrointestinal bleeding has been improved by adopting an individualized treatment plan according to evidence-based methods.


Zhiyuan Q.,Soochow University of China | Qingyong L.,Soochow University of China | Shengming H.,Central Hospital of Xianning | Hui M.,First Peoples Hospital of Jining
Brain Injury | Year: 2016

The goal of this study was to investigate the protective effect of recombinant human EPO(rhEPO) on cerebral microvascular endothelial cells and the mechanisms by which rhEPO interacts with TJs proteins, claudin-5, Occludin and ZO-1 during the early period following traumatic brain injury.Research design: Rats (n = 81) were randomly divided into sham-operated group, TBI group and rhEPO+TBI group. Traumatic brain injury was induced by the Marmarou method.Methods and procedures: Rats were killed at 3, 24, 72 and 168 hours after TBI. The integrity of the blood-brain barrier was investigated by using a spectrophotometer to assess extravasation of Evans blue dye. The expression of Claudin-5, Occludin and ZO-1 were determined by immunohistochemistry and real-time fluorescence quantitative PCR.Results: From 3 hours to 3 days, rats in the TBI group demonstrated a remarkable increase in Evans blue content in the brain, relative to rats in the sham-operated group (p < 0.05). The expression of Claudin-5 and Occludin was significantly lower than those in the sham-operated group (p < 0.05). In contrast, rats in the TBI+rhEPO group demonstrated a significant decrease in brain levels.Conclusion: It was found that administration of rhEPO protected cerebral microvascular endothelial cells and reduced permeability of BBB and the mechanisms may be due to increasing the expression of TJs proteins. © 2016 Taylor and Francis Group, LLC.


Li M.,University of Science and Technology of China | Li S.,University of Science and Technology of China | Yu L.,University of Science and Technology of China | Wu J.,University of Science and Technology of China | And 5 more authors.
PLoS ONE | Year: 2013

Objectives: Recent findings suggest that in response to repair-to-injury bone marrow mesenchymal stem cells (BMSCs) participate in the process of angiogenesis. It is unclear what role BMSCs play in the structure of the vessel wall. In present study, we aimed to determine whether BMSCs had the capacity of endothelial cells (ECs). Methods: BMSCs were separated and cultured. FACS and RT-PCR analysis confirmed the gene expression phenotype. The capacity of migration and adhesion and the ultrastructure of BMSCs were examined. The effect of BMSCs transplantation on the vascular repair was investigated in a murine carotid artery-injured model. Results: BMSCs could express some markers and form the tube-like structure. The migration and adhesion capacity of BMSCs increased significantly after stimulated. In addition, BMSCs had the intact cell junction. In vivo the local transfer of BMSCs differentiated into neo-endothelial cells in the injury model for carotid artery and contributed to the vascular remodeling. Conclusion: These results showed that BMSCs could contribute to neointimal formation for vascular lesion and might be associated with the differentiation into ECs, which indicated the important therapeutic implications for vascular diseases. : © 2013 Li et al.

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