Xianning, China
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PubMed | Wuhan University of Science and Technology, University of Science and Technology of China and Xianning Central Hospital
Type: Journal Article | Journal: Experimental cell research | Year: 2016

Uterine fibroids, also known as uterine leiomyomas, are a benign tumor of the human uterus and the commonest estrogen-dependent benign tumor found in women. Myocardin is an important transcriptional regulator in smooth and cardiac muscle development. The role of myocardin and its relationship with ER in uterine fibroids have barely been addressed. We noticed that the expression of myocardin was markedly reduced in human uterine fibroid tissue compared with corresponding normal or adjacent myometrium tissue. Here we reported that myocardin induced the transcription and expression of differentiation markers SM22 and alpha smooth muscle actin (-SMA) in rat primary uterine smooth muscle cells (USMCs) and this effect was inhibited by ER. Notably, we showed that, ER induced expression of proliferation markers PCNA and ki-67 in rat primary USMCs. We also found ER interacted with myocardin and formed complex to bind to CArG box and inhibit the SM22 promoter activity. Furthermore, ER inhibited the transcription and expression of myocardin, and reduced the levels of transcription and expression of downstream target SM22, a SMC differentiation marker. Our data thus provided important and novel insights into how ER and myocardin interact to control the cell differentiation and proliferation of USMCs. Thus, it may provide potential therapeutic target for uterine fibroids.


PubMed | Hubei University of Medicine, Hubei University and Xianning Central Hospital
Type: Journal Article | Journal: Archives of medical research | Year: 2016

AAA+nuclear coregulatory cancer associated (ANCCA) has been demonstrated as playing important roles in diverse biological processes including tumorigenesis. However, the clinical and prognostic significance of ANCCA in squamous cell lung carcinoma (SCLC) patients is still unknown. The aim of this study is to identify the role of ANCCA in SCLC patients.ANCCA mRNA and protein expressions were detected in SCLC tissues and cell lines by real-time PCR and Western blot. We examined the ANCCA protein expression in 152 SCLC samples by immunohistochemistry and analyzed the association between the expression of ANCCA protein and clinicopathological characteristics of SCLC patients.ANCCA mRNA and protein expression are increased in SCLC tissues and cell lines. Moreover, ANCCA protein overexpression was associated with differentiated degree, clinical stage, lymph node metastasis, and distant metastasis. In uni- and multivariate analyses, ANCCA protein overexpression was an independent poor prognostic factor for SCLC patients.ANCCA act as a potential biomarker for therapeutic strategy and prognostic prediction for SCLC.


Chen X.,University of Science and Technology of China | Cai F.,University of Science and Technology of China | Guo S.,University of Science and Technology of China | Ding F.,Xianning Central Hospital | And 3 more authors.
Alcoholism: Clinical and Experimental Research | Year: 2014

Background: The effect of Flos Puerariae extract (FPE) on alcohol metabolism, hepatic injury, and memory impairment was assessed following acute ethanol (EtOH) intoxication in mice. Methods: The model of acute EtOH intoxication was established by intragastric administration with 8 g/kg EtOH in mice. FPE was orally administrated (gavage) once a day for 7 consecutive days. Mice were randomly divided into 4 groups: control group, model group, and FPE groups (100, 200 mg/kg). Alcohol tolerance and intoxication time, blood alcohol concentration, the activities of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) in liver, aspartate amino transferase (AST) and alanine amino transferase (ALT) in serum, superoxide dismutase (SOD), glutathione peroxidase (GSH-px), catalase and the formation of malondialdehyde (MDA) in both liver and brain, as well as memory ability were determined after acute alcohol exposure. Results: Compared with model group, pretreatment with FPE significantly prolonged alcohol tolerance time and shortened intoxication time, which is accompanied by decreased blood alcohol concentration and elevated activities of ADH and ALDH in liver. Moreover, the index of hepatic injury, ALT, and AST activities in serum was markedly decreased by pretreatment with FPE. Additionally, decreased MDA level, enhanced GSH-px and catalase activities in liver, as well as enhanced SOD and catalase activities in brain were found in FPE pretreated mice after acute exposure to EtOH. Furthermore, FPE pretreated mice showed markedly relieved memory disruption following acute EtOH intoxication. Conclusions: This study suggests that FPE pretreatment could enhance alcohol metabolism, prevent hepatic injury, and relieve memory impairment after acute alcohol intoxication and that this effect is likely related to its modulation on the alcohol metabolizing and antioxidant enzymes. © 2014 by the Research Society on Alcoholism.


Wang D.,Xianning Central Hospital | Hao T.,Xianning Central Hospital | Pan Y.,Xianning Central Hospital | Qian X.,Xianning Central Hospital | Zhou D.,Huazhong University of Science and Technology
Molecular and Cellular Biochemistry | Year: 2015

The aim of the present study was to analyze the expression of sex-determining region Y-related high mobility group box 4 (SOX4) in non-small cell lung cancer (NSCLC) and its correlation with clinicopathologic characteristics, including the survival of NSCLC patients. To observe initially the expression status of SOX4 in lung squamous cell carcinoma and adenocarcinoma at gene expression omnibus. The expression of SOX4 mRNA and protein was examined in NSCLC tissues and normal lung tissues through real-time PCR and immunohistochemistry. Meanwhile, the relationship of SOX4 expression levels with clinical characteristics of 168 NSCLC patients was analyzed by immunohistochemistry. Univariate and multivariate analyses were performed to determine the association between SOX4 expression and prognosis of NSCLC patients. In our results, SOX4 expression was increased in NSCLC tissues compared with paired normal lung tissues in microarray data (GSE3268). SOX4 mRNA and protein expression were markedly higher in NSCLC tissues than in normal lung tissues (P = 0.001 and P = 0.001, respectively). Using immunohistochemistry, high levels of SOX4 protein were positively correlated with status of differentiated degree (high vs. middle, P = 0.004; high vs. low, P < 0.001), clinical stage (I–II vs. III–IV, P < 0.001), T classification (T1–T2 vs. T3–T4, P = 0.004), N classification (N0–N1 vs. N2–N3, P = 0.002), and M classification (M0 vs. M1, P = 0.011) in NSCLC. Moreover, the higher level of SOX4 expression was markedly correlated with poor overall survival in NSCLC patients (P < 0.001). Multivariate analysis suggested that increased SOX4 expression was a poor independent prognostic predictor for NSCLC patients (P = 0.002). In conclusion, SOX4 plays an important role on NSCLC progression and prognosis and may serve as a convictive prognostic biomarker for NSCLC patients. © 2015, Springer Science+Business Media New York.


Zhou L.-B.,Xianning Central Hospital | Wu J.-Y.,Xianning Central Hospital
World Chinese Journal of Digestology | Year: 2016

AIM To identify the risk factors for post-endoscopic sphincterotomy (EST) biliary tract infection in patients with choledocholithiasis. METHODS From May 2012 to May 2015, 102 patients with choledocholithiasis were chosen as study subjects, and all patients were treated by EST. These patients were divided into an infection group and a control group. Pre-operative data, operative data, basic diseases and history were compared between the two groups to identify the risk factors for biliary tract infection after EST. RESULTS A total of 29 patients were diagnosed with biliary tract infection. Diameter of stones ≥ 25 mm (OR = 1.971, 95%CI: 0.975-3.489), biliary dilatation/stricture (OR = 2.971, 95%CI: 1.165-4.724), multiple ERCP (OR = 2.217, 95%CI: 1.019-4.876), lithotripsy (OR = 2.598, 95%CI: 1.147-4.790), operative time ≥ 60 min (OR = 2.087, 95%CI: 1.994-3.867), diabetes (OR = 2.176, 95%CI: 1.763-3.885), biliary operation (OR = 1.723, 95%CI: 1.357-3.109), ERCP history (OR = 2.089, 95%CI: 1.665-3.576) and biliary stent (OR = 2.219, 95%CI: 1.982-3.347) were risk factors for post-EST biliary tract infection. Usage of antibiotics was identified to be a protective factor for post-EST biliary tract infection (OR = 0.471, 95%CI: 0.231-0.796). CONCLUSION Post-EST biliary tract infection demonstrates significant relativity with multiple factors, while usage of antibiotics exerts a protective effect against the infection. © 2016 Baishideng Publishing Group Inc. All rights reserved.


Xing Z.,Huazhong University of Science and Technology | Yu G.,Xianning Central Hospital | Lv C.,Xianning Central Hospital | Qin L.,Xianning Central Hospital | Zhao H.,Huazhong University of Science and Technology
International Journal of Clinical and Experimental Pathology | Year: 2016

Neuroglioma is the most common intracranial tumor in China. MicroRNA (miR) has been recognized as one novel drug reagent in clinics. This study thus focused on the role of miR-129 on neuroglioma cells. Quantitative PCR (qPCR) was used to detect the expression of miR-129 in human neuroglioma cells. In neuroglioma cell line U87, miR-129 was over-expressed or silenced. Western blotting was employed to test the expression of nuclear factor (NF)-?B. Meanwhile immunofluorescent assay detected the nuclear p65 expression to confirm the correlation between miR-129 and NF-kB. Further proliferation and scratch assay were performed to detect the role of miR-129 on cellular growth of neuroglioma cells. qPCR results showed elevated expression of miR-129 in human neuroglioma cells. In glioma cell line with miR-129 overexpression, pP65 protein level was elevated. The knockdown of miR-129 led to decreased pP65 protein (P<0.01). Immunofluorescent assay showed the nuclear entry of pP65, indicating the activation of NF-?B signal by miR-129 over-expression. Further MTT and scratch assay showed the rapid proliferation and enhanced invasion assay of U87 neuroglioma cells under miR-129 over-expression (P<0.01). The silencing of miR-129 also weakened cell proliferation and mobility (P<0.01). MiR-129 can activate NF-kB signal in neuroglioma cells for enhancing cell proliferation.


Wang X.,Xianning Central Hospital | Zhu T.-J.,Xianning Central Hospital | Zhou X.-F.,Xianning Central Hospital | Wan Z.-T.,Xianning Central Hospital
Chinese Journal of Contemporary Pediatrics | Year: 2015

Objective: To investigate the association of single nucleotide polymorphisms (SNP) rs22833188 and rs2833195 in TIAM1 gene with the susceptibility to Kawasaki disease (KD) and its clinical characteristic in children. Methods: A case-control study was performed in this study. One hundred and eighty-eight children with KD and 197 normal children served as controls were enrolled. The genotypes of two SNPs rs22833188 and rs2833195 in TIAM1 gene were detected using PCR-RFLP. Results: There were no significant differences in the genotype (AA, AG and GG) and allele frequencies of SNP rs2833188 between the KD and control groups. Significant differences in the genotype (CC, GC and GG) frequency of SNP rs2833195 were noted between the KD and control groups (P=0.017). The frequency of C allele in the KD group was higher than in the control group (P=0.015). The polymorphism of SNP rs2833188 was associated with the occurrence of rash (P=0.011), and the polymorphism of SNP rs2833195 was associated with the occurrence of conjunctival hyperemia (P=0.021). Conclusions: The polymorphism of rs2833195 in TIAM1 gene is associated with the susceptibility to KD. The polymorphisms rs2833188 and rs2833195 in TIAM1 gene may be associated with some clinical characteristics in children with KD.


PubMed | Xianning Central Hospital
Type: Journal Article | Journal: Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics | Year: 2015

To investigate the association of single nucleotide polymorphisms (SNP) rs22833188 and rs2833195 in TIAM1 gene with the susceptibility to Kawasaki disease (KD) and its clinical characteristic in children.A case-control study was performed in this study. One hundred and eighty-eight children with KD and 197 normal children served as controls were enrolled. The genotypes of two SNPs rs22833188 and rs2833195 in TIAM1 gene were detected using PCR-RFLP.There were no significant differences in the genotype (AA, AG and GG) and allele frequencies of SNP rs2833188 between the KD and control groups. Significant differences in the genotype (CC, GC and GG) frequency of SNP rs2833195 were noted between the KD and control groups (P=0.017). The frequency of C allele in the KD group was higher than in the control group (P=0.015). The polymorphism of SNP rs2833188 was associated with the occurrence of rash (P=0.011), and the polymorphism of SNP rs2833195 was associated with the occurrence of conjunctival hyperemia (P=0.021).The polymorphism of rs2833195 in TIAM1 gene is associated with the susceptibility to KD. The polymorphisms rs2833188 and rs2833195 in TIAM1 gene may be associated with some clinical characteristics in children with KD.


PubMed | Xianning Central Hospital
Type: Journal Article | Journal: Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics | Year: 2015

To study the association of single nucleotide polymorphisms (SNP) (rs2393903 and rs10995251) in ZNF365 gene with bronchial asthma and its clinical characteristics in Han Chinese children in Hubei, China.A total of 221 children with bronchial asthma and 243 normal children, all of whom were from Hubei, were recruited to carry out a case-control study. The genotype and allele frequencies of two SNPs in ZNF365 gene were determined using the polymerase chain reaction-restriction fragment length polymorphism technique.There were no significant differences in the distribution of three genotypes (GG, GA, AA) and allele frequency in SNP rs2393903 between the asthma and control groups (P>0.05). However, there were significant differences in the distribution of three genotypes (CC, CT, TT) and allele frequency in SNP rs10995251 between the asthma and control groups (P<0.05); C allele was a risk factor (OR=1.380). The asthmatic children with CC genotype of SNP rs10995251 had a significantly higher serum level of total immunoglobulin E (IgE) than those with TT genotype (P<0.05).The SNP rs10995251 in ZNF365 gene is associated with the susceptibility to bronchial asthma in children in Hubei, China, and the SNP may affect the level of serum IgE in these children.


PubMed | Xianning Central Hospital
Type: Journal Article | Journal: Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics | Year: 2016

To investigate the association of two single-nucleotide polymorphisms (SNPs) in IL1R1 gene (rs1558641 and rs949963) with the susceptibility to asthma in children from Central China.A case-control study was performed in the asthma group and the control group, consisting of 208 children with asthma and 223 normal children from Central China, respectively. The genotypes of two SNPs in IL1R1 gene, rs1558641 and rs949963, were identified using polymerase chain reaction-restriction fragment length polymorphism. The serum level of IL1R1 was determined by enzyme-linked immunosorbent assay.There were no significant differences in genotype and allele frequencies of rs1558641 between the asthma and control groups. In terms of rs949963, the frequencies of GG genotype and alleles were significantly higher in the asthma group than in the control group (P<0.05). The asthma group had a significantly higher serum level of IL1R1 than the control group (P=0.011). Moreover, the serum level of IL1R1 was significantly higher in patients with GG genotype than in those with AA or AG genotype for rs949963 (P=0.028).IL1R1 SNP rs949963 is associated with the susceptibility to asthma in children from Central China and may increase the serum expression of IL1R1.

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