Yu G.,Xianning Center Hospital |
Zhu P.,Chongqing Medical University |
Zhang J.,Chongqing Medical University |
Wang J.,Chongqing Medical University
Medical Journal of Wuhan University
Objective: To discuss the expression of vascular endothelial growth-C (VEGF-C) in gastric carcinomas tissue and its relation with tumor lymphangiogenesis; to observe the expression of VEGF-C protein on human gastric cancer cell line SGC-7901 which had been transfected by anti-sense VEGF-C based on the carrier of pCI-neo, and to investigate whether it could inhibit the growth of human gastric carcinoma cell line in vitro. Methods: The expressions of VEGF-C mR-NA in 72 gastric carcinoma tissue specimens were determined by RT-PCR. Immunohistochemis try was used to detect the expression of VEGFR-3 in these tissues as well. Lymphatic vessel density (LVD) was counted. Using the liposome infection protocol which had been reported previously, the plasmid of pCI-neo anti-sense VEGF-C was transfected into human gastric cancer cell line SGC-7901, then immunohistochemical and Western blotting were used to detect target genes and its expression. The cell viability was detected by MTT assay. Results: The positive expression rate of VEGF-C in positive lymph node was significantly higher than that in negative lymph node (85.7% vs 20.0%, P < 0.05). Compared with that in lymph node without metastasis, LVD in positive lymph node increased significantly (6.65 ± 1.57 vs 3.75 ± 1.47, P < C0.05). LVD in positive VEGF-C tissue was significantly higher than that in negative VEGF-C tissue (6.36 ± 1.66 vs. 3.95 ± 1.52, P < 0.05). The immunohistochemical & Western blotting results showed that there was no expression of VEGF-C mRNA or protein in the transfected group. MTT assay revealed that the transfected group had a lower cell viability than the other groups (P < 0.05). Conclusion: VEGF-C can promote the invasion of gastric carcinoma and lymphatic proliferation. The expression of VEGF-C mRNA and its protein in SGC-7901 cells could be inhibited by pCI-neo-antisense VEGF-C through transfection. The study showed that anti-sense VEGF-C gene could suppress the growth of human gastric cells in vitro as well. Source
Chen Y.,Xianning Center Hospital |
Gan S.,Xianning Center Hospital
Medical Journal of Wuhan University
Objective: To study the effect of coronary angiography on Cystatin C and renal function in patients with diabetes, and to explore the risk factors and prevention methods methods of contrast induced nephropathy (CIN). Methods: A total of 78 patients, who had been successfully carried on coronary angiography, were divided into diabetes group (n = 42) and control group (n = 36). Cystatin C and renal function were detected before and 1, 2, 7 days after coronary angiography. Results: Cystatin C increased significantly in diabetic patients one day after angiography than in normal control patients (P<0. 05). Ten of 78 patients suffered from CIN including 8 diabetic patients and 2 control patients. Conclusion: Coronary angiography can cause a momentary renal damage, and diabetes is one of risk factors for coronary angiography. Source
Jiang B.,Xianning Center Hospital |
Chen Q.,Xianning Center Hospital |
Liu X.,Wuhan University |
Kong D.,Xianning Center Hospital |
And 3 more authors.
Objectives: Ischemia-reperfusion (I/R) injury is the most common cause of renal dysfunction. Ischemic postconditioning (IPO) is a phenomenon by which intermittent interruptions of blood flow in the early phase of reperfusion protect an organ from I/R injury. In the present study, we investigated the effects of IPO on renal I/R injury using a canine autotransplantation model. Materials and methods: Fifty adult male mongrel dogs were randomly divided into five groups of 10 animals each. The animals underwent a left nephrectomy followed by flushing and static preservation of the kidney in University of Wisconsin solution for 24 hours. IPO was performed by six cycles of 10 or 30 seconds or three cycles of 1-minute I/R before final reperfusion. All dogs underwent a right nephrectomy 24 hours later followed by autotransplantation of the preserved left kidney. Blood and urine were collected at various reperfusion times (24, 48, and 72 hours). We assayed blood urea nitrogen and creatinine levels, as well as urine N-acetyl-β-D-glucosaminidase levels. Kidney samples were harvested after I/R to measured renal superoxide dysmutase (SOD), malondialdehyde (MDA), and myeloperoxidase (MPO) concentrations. Apoptosis was evaluated by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling assays of tissue samples. Results: Compared with the sham group, I/R resulted in renal dysfunction with decreased SOD and increased MDA and MPO levels, as well as increased apoptosis indices. However, IPO attenuated the above effects, particularly the six cycles of 10-second I/R. Conclusions: IPO exerted protective effects on renal I/R injury. © 2012 Elsevier Inc. All rights reserved. Source
Zhao W.-H.,Xianning Center Hospital |
Qu X.-F.,University of South China |
Xing Z.-G.,Xianning Center Hospital |
Zhao L.-Q.,University of South China |
And 2 more authors.
Journal of B.U.ON.
Purpose: Mutation and polymorphism of Kras oncogene are considered as candidate risk factor and drug response predictor for cancer. However, the conclusions of accumulating reports related to the relationship of rs712 of Kras gene and risk of cancer remain nuclear. Methods: We performed a meta-analysis including 6 eligible studies containing 1661 cases and 2139 controls to explore the role ofrs712 in the risk of cancer development. Results: Meta-analysis results showed that rs712 allele T (PH=0.08, odds ratio/OR=l.35, 95% confidence inter- CI=1.60-3.37), and allele T carrier genotype (GT/TT) (PH=0.14, OR=1.30, 95% CI=1.10-1.55) were strongly associated with cancer in Chinese population. No evidence of association was observed between rs712 and risk of cancer in overall population. Conclusion: The findings suggested that allele T, genotype TT and allele T carrier (GT/TT) ofrs712 may increase susceptibility to cancer risk in Chinese population, and can be used as a genetic factor for evaluating risk of cancer. Source