Zhao M.,Xian Jiaotong University |
Yang Y.,Xian Jiaotong University |
Bi X.,Xian Jiaotong University |
Yu X.,Xian Jiaotong University |
And 3 more authors.
Cellular Physiology and Biochemistry | Year: 2015
Background: Previous studies have shown that inflammation is associated with excessive activation of calpains. Acetylcholine (ACh) has been reported to inhibit pro-inflammatory cytokine release and protect against cardiomyocyte injury. However, there is no direct evidence regarding whether ACh can regulate calpains to exert cardioprotection. To this end, we investigated the effect of ACh on tumour necrosis factor alpha (TNF-)-induced cardiomyocyte injury and further explored the underlying mechanism. Methods: Flow cytometry and transmission electron microscopy were performed to evaluate apoptosis and cellular ultrastructure. Western blotting was performed to assess changes in protein expression. siRNA was employed to silence specific proteins. Results: TNF- treatment increased the expression of cleaved caspase-3, calpain-1 and p38-mitogen-activated protein kinase (p38-MAPK). The calpain inhibitor PD150606 and the p38-MAPK inhibitor SB203580 inhibited apoptosis induced by TNF-. Moreover, SB203580 decreased the expression and activity of calpain-1, possibly related to the up-regulation of calpastatin. ACh significantly inhibited TNF--induced cell apoptosis, as evidenced by decreases in caspase-3 cleavage, p38-MAPK phosphorylation, and calpain-1 expression and activity as well as increases in calpastatin expression. These beneficial effects of ACh were abolished by atropine or M2AChR siRNA. Conclusion: Our results suggest that ACh ameliorated TNF--induced calpain activation by decreasing p38-MAPK phosphorylation and enhancing calpastatin expression, indicating that calpain may be an important link between inflammatory factors and myocardial cell apoptosis. © 2015 S. Karger AG, Basel.
Yuan Z.,Northwestern Polytechnical University |
Yuan Z.,Xian Peihua University |
Huo S.-H.,Northwestern Polytechnical University |
Geng X.-L.,Northwestern Polytechnical University
Journal of Central South University | Year: 2014
Stability analyses of perfect and imperfect cylindrical shells under axial compression and torsion were presented. Finite element method for the stability analysis of perfect cylindrical shells was put forward through comparing critical loads and the first buckling modes with those obtained through theoretical analysis. Two typical initial defects, non-circularity and uneven thickness distribution, were studied. Critical loads decline with the increase of non-circularity, which exist in imperfect cylindrical shells under both axial compression and torsion. Non-circularity defect has no effect on the first buckling mode when cylindrical shell is under torsion. Unfortunately, it has a completely different buckling mode when cylindrical shell is under axial compression. Critical loads decline with the increase of thickness defect amplitude, which exist in imperfect cylindrical shells under both axial compression and torsion, too. A greater wave number is conducive to the stability of cylindrical shells. The first buckling mode of imperfect cylindrical shells under torsion maintains its original shape, but it changes with wave number when the cylindrical shell is under axial compression. © 2014 Central South University Press and Springer-Verlag Berlin Heidelberg.
Pan T.-J.,Changan University |
Qiu Y.-B.,Changan University |
Zheng H.,Changan University |
Zhao R.,Xian Peihua University
Advanced Materials Research | Year: 2014
Stiffing steel plate deep beam is introduced as a new lateral force resistant structure system and put forward mechanism of stiffing steel plate deep beam. Mechanical property of stiffing steel plate deep beam with different span-depth ratio under horizontal low cyclic load is analyzed through ANSYS which is a software of finite element analysis. The results indicates that with decreasing of span-depth ratio αultimate load raised substantially and strength of component gradually changes from depending on bending to shearing. The optimal value of span-depth ratio α is 1.5. © (2014) Trans Tech Publications, Switzerland.
Wei G.-F.,Xian University of Arts and Science |
Chen W.-L.,Xian Peihua University |
Dong S.-H.,National Polytechnic Institute of Mexico |
Dong S.-H.,Louisiana State University
Physics Letters, Section A: General, Atomic and Solid State Physics | Year: 2014
Within the framework of partial-wave method, we study in this Letter the arbitrary l continuum states of the Schrödinger equation with the hyperbolic molecular potential in terms of an improved approximation to the centrifugal term. We present the normalized radial wave functions and obtain analytical formula of phase shifts. In addition, the corresponding bound states are also discussed by studying the analytical properties of the scattering amplitude. We calculate the energy spectra and scattering phase shifts by the improved, previous approximations and the accurate methods, respectively and find that the improved approximation is better than the previous one since the present results are in better agreement with the accurate ones. © 2014 Elsevier B.V.
Chen W.-L.,Xian Peihua University |
Wei G.-F.,Xian University of Arts and Science
Chinese Physics B | Year: 2011
By applying a Pekeris-type approximation to the centrifugal term, we study the spin symmetry of a Dirac nucleon subjected to scalar and vector modified Rosen-Morse potentials. A complicated energy equation and associated two-component spinors with arbitrary spin-orbit coupling quantum number k are presented. The positive-energy bound states are checked numerically in the case of spin symmetry. The relativistic modified Rosen-Morse potential cannot trap a Dirac nucleon in the limiting case α → 0. © 2011 Chinese Physical Society and IOP Publishing Ltd.