Xian Medical University
Xian Medical University
Zhang S.,U.S. National Institutes of Health |
Qi J.,U.S. National Institutes of Health |
Li X.,U.S. National Institutes of Health |
Li X.,Xian Medical University |
And 7 more authors.
Nature Neuroscience | Year: 2015
Mesoaccumbens fibers are thought to co-release dopamine and glutamate. However, the mechanism is unclear, and co-release by mesoaccumbens fibers has not been documented. Using electron microcopy, we found that some mesoaccumbens fibers have vesicular transporters for dopamine (VMAT2) in axon segments that are continuous with axon terminals that lack VMAT2, but contain vesicular glutamate transporters type 2 (VGluT2). In vivo overexpression of VMAT2 did not change the segregation of the two vesicular types, suggesting the existence of highly regulated mechanisms for maintaining this segregation. The mesoaccumbens axon terminals containing VGluT2 vesicles make asymmetric synapses, commonly associated with excitatory signaling. Using optogenetics, we found that dopamine and glutamate were released from the same mesoaccumbens fibers. These findings reveal a complex type of signaling by mesoaccumbens fibers in which dopamine and glutamate can be released from the same axons, but are not normally released at the same site or from the same synaptic vesicles. © 2015 Nature America, Inc. All rights reserved.
Jia X.,Xian Medical University
International Journal of Grid and Distributed Computing | Year: 2016
The project deployment diagram, which is used to uniformly describe the process and resource of virtual enterprise is established aiming at how to choose virtual enterprise partner of time-cost tradeoff and set task-resource assignment graph as scheduling model, the iterative heuristic algorithm is adopted based on relative cost-effectiveness to solve. The production of virtual partner and transportation time and cost are taken into account in algorithm, which may ensure deadline constraint as well as effectively cut down overall cost. Such algorithm applying to resource distribution and a vast amount of simulative tests proved that this method is able to effectively cut down the operation time of obtaining prioritization scheme. © 2016 SERSC.
Naichun J.,Xian Medical University
Advances in Intelligent Systems and Computing | Year: 2018
Sports biomechanics analysis not only plays an important role in the practice of human body movement and a powerful tool for athletes and coaches, but also has a wide range of applications in the field of national defense, medicine, aerospace, industry and so on. This paper introduces the technology of sports biomechanics system based on computer-aided analysis and to the knee. The measurement of the rotational stability of the knee joint was simulated using finite element method. The results of the direct athletes training intensity, angle and intensity have a certain reference value. © 2018, Springer International Publishing AG.
Tu Y.,Fourth Military Medical University |
Gao X.,Fourth Military Medical University |
Gao X.,Xian Medical University |
Gao X.,Shanghai Institute of Technology |
And 7 more authors.
Cancer Research | Year: 2013
Malignant gliomas are the most common central nervous system tumors and the molecular mechanism driving their development and recurrence is still largely unknown, limiting the treatment of this disease. Here, we show that restoring the expression of miR-218, a microRNA commonly downregulated in glioma, dramatically reduces the migration, invasion, and proliferation of glioma cells. Quantitative reverse transcription PCR and Western blotting analysis revealed that expression of the stem cell-promoting oncogene Bmi1 was decreased after overexpression of miR-218 in glioma cells. Mechanistic investigations defined Bmi1 as a functional downstream target of miR-218 through which miR-218 ablated cell migration and proliferation. We documented that miR-218 also blocked the self-renewal of glioma stem-like cells, consistent with the suggested role of Bmi1 in stem cell growth. Finally, we showed that miR-218 regulated a broad range of genes involved in glioma cell development, including Wnt pathways that suppress glioma cell stem-like qualities. Taken together, our findings reveal miR-218 as a tumor suppressor that prevents migration, invasion, proliferation, and stemlike qualities in glioma cells. © 2013 American Association for Cancer Research.
Jia D.,PLA Fourth Military Medical University |
Han B.,Xian Medical University |
Yang S.,First Peoples Hospital of Xianyang |
Zhao J.,Xian Medical University
Journal of Molecular Neuroscience | Year: 2014
In the present study, we aimed at evaluating the potential neuroprotective effect and the underlying mechanism of anemonin against cerebral ischemia and reperfusion (I/R) injury. Anemonin was administered to rats by the intraperitoneally (i.p.) route once daily for 7 days before middle cerebral artery occlusion (MCAO). Focal cerebral ischemia was induced by 90 min of MCAO followed by 24 h of reperfusion. After that, animals were sacrificed by decapitation, brain was removed, and various biochemical estimations, neurological status, and assessment of cerebral infarct size were carried out. MCAO followed by 24 h of reperfusion caused a significant increase in infarct size, neurological deficit score, malondialdehyde (MDA) content, reactive oxygen species (ROS) level, and DNA fragmentation, as well as a decrease in the activities of superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), glutathione peroxidase (GPx), and Na+, K+-ATPase in the brain. Furthermore, elevated Bax expression, increased caspase-3 cleavage, and decreased Bcl-2 expression were observed in nontreated rats in response to focal cerebral I/R injury. However, pretreatment with anemonin significantly reversed these levels of biochemical parameters, reduced cerebral infarct size, and improved the neurologic score in cerebral ischemic animals. Additionally, a wide distribution of anemonin in plasma and brain tissues and the brain-to-plasma partition coefficient (Ri) ratio of 0.7 at 90 min indicated that this compound could penetrate the blood-brain barrier (BBB). These results showed that pretreatment with anemonin provided a significant protection against cerebral I/R injury in rats by, at least in part, its antioxidant action and consequent inhibition of apoptosis. © 2014 Springer Science+Business Media.
Xu T.,Xian Medical University
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2013
To investigate the combined effects of insulin and selenium in improving the physiological parameters and insulin signal transduction in the skeletal muscles of diabetic rats. 35 male SD rats were randomly divided into five groups: normal, diabetes, diabetes with insulin treatment (D-In group), diabetes with sodium selenite treatment (D-In-S group), and diabetes with insulin and sodium combination treatment (D-In-Se group), 7 rats in each group. The levels of blood glucose were measured using One Touch Sure Step Blood Glucose Meter. HbA1C levels were measured using microcolumn assay. The levels of insulin receptor substrate 1(IRS-1), phosphatidylinositol-3 kinase (PI3K) and glucose transporter 4(GLUT4) in skeletal muscle were examined by Western blotting and immunohistochemistry. Insulin combined with selenium could significantly lower blood glucose levels and markedly restore the diminished expression of in IRS-1, PI3K and GLUT4 levels in skeletal muscles of diabetic rats. There was positive cooperativity between insulin and selenium in reducing blood glucose levels in diabetic rats. The combined treatment of insulin and selenium may decrease blood glucose by upregulating IRS-1, PI3K and GLUT4 levels in skeletal muscles of diabetic rats.
Zeng J.R.,Xian Medical University
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2012
To observe the expression changes of tumor necrosis factor alpha (TNF-α) and interleukin-10 (IL-10) during the developing process of pressure overload-induced myocardial remodeling in rats, and investigate the dynamic correlation between TNF-α and left ventricular mass index (LVMI) as well as myocardial collagen volume fraction (CVF). The rats (n=56) were randomly divided into control group, sham operation group and model group. Animal models were induced by abdominal aortic constriction (AAC). AAC group was further divided into 2 weeks, 4 weeks, 8 weeks and 12 weeks groups. Dynamic expression changes of TNF-α and IL-10 were tested by enzyme-linked immunosorbent assay(ELISA), and myocardial collagen fiber was observed by Mallory's staining. Myocardial cells became increasingly disarranged, filaments broken, intercellular space increased, and collagen fiber accumulated 4 weeks after operation. Furthermore, heart failure occurred 12 weeks after operation. ABC-ELISA results showed that TNF-α expression in myocardium increased at 4, 8, 12-week operation groups in a time-dependent fashion compared with control group (P<0.01); Correlation analysis indicated the expression of TNF-α in myocardium was positively correlated with LVMI (r=0.582, P<0.01) and CVF (r=0.932, P<0.01); IL-10 expression in myocardium among groups were similar, but the ratio of TNF-α and IL-10 increased in a time-dependent manner, from (1.79 ± 0.19) ng/mL (2 weeks) up to (2.85 ± 0.24) ng/mL(12 weeks) as compared with control group (1.74 ± 0.24) ng/mL (P<0.01). The degree of myocardial remodeling plays a key role in heart function deterioration, and the TNF-α expression up-regulation in a time-dependent manner and the disproportion of TNF-α and IL-10 is one of important molecular mechanisms.
Nan F.,Xian Medical University
International Journal of Control and Automation | Year: 2016
With the features of high density, speed and too many restrictions, train operation adjustment on double track-line is a typical large-scale combinatorial optimization problem and the NP-hard problem. In this paper, based on the characteristics of China's railway operation, a mathematical model is built to shorten the train group's total delayed time and reduce the delayed time to the minimum, and which is solved and optimized with differential evolution algorithm. As the standard differential evolution algorithm can easily lead to the problem of 'premature convergence', an improved differential evolution algorithm is put forward. In the improved algorithm, the adaptive nonlinear regressive mutation operator and crossover operator of t-distribution are adapted to improve the mutation and the design of crossover operator in the standard differential evolution algorithm. The experimental results show the improved differential evolution algorithm has a high rationality and feasibility in solving the problem of train operation adjustment on double track-line, and it has better results than applying the genetic algorithm and standard differential evolution algorithm. © 2016 SERSC.
Huo J.,Xian Medical University
Artificial Cells, Nanomedicine and Biotechnology | Year: 2016
To observe the changes in invasion capacity of gastric cancer BGC823 cells after being treated with chitosan-encapsulated BRAF siRNA nanoparticles, and to evaluate the effects of the nanoparticle-mediated BRAF siRNA interference on cell invasion and metastasis, BRAF siRNA was encapsulated with chitosan into nanoparticles sized 350 nm to treat gastric cancer cells. Silencing of BRAF was detected by Western blot and PCR, and cell invasion was observed by the Transwell assay. The nanoparticles significantly downregulated BRAF expression in BGC823 cells (P < 0.01) and inhibited their invasion (P < 0.001). Chitosan nanoparticle-mediated BRAF siRNA interference evidently reduced the invasion capacity of gastric cancers. © 2015 Informa Healthcare USA, Inc.
Bei Q.,Xian Medical University
Jiegou Huaxue | Year: 2012
A new asymmetric bidentate copper(II) complex, CuL2 (HL = 2-((E)-(4-bromophenylimino) methyl)-6-bromo-4-chlorophenol), has been synthesized and characterized by elemental analyses and single-crystal X-ray diffraction. The complex crystallizes in the monoclinic space group P2 1/c with a = 11.218(3), b = 9.355(3), c = 13.449(4) Å, β = 108.722(4)°, V = 1336.8(6) Å 3, Z = 2, D c = 2.008 g/cm 3, μ(MoKa) = 7.024 mm -1, F(000) = 806, S = 0.999, the final R = 0.0342 and wR = 0.0641 for 2611 observed reflections (I > 2s(I)). The central copper(II) is four-coordinate and bonds to two nitrogen and two oxygen atoms from two Schiff base ligands. The complex is linked into a two-dimensional supramolecular structure by weak intermolecular interactions. In addition, DNA-binding properties of the metal complex were investigated using spectrometric titrations and viscosity measurements. The results show that the complex binds with calf-thymus DNA(CT-DNA), presumably via a partial intercalative mode. The intrinsic binding constant of the Cu(II) complex with DNA is 7.335×10 3 M -1. Copyright © 2008 Chinese Journal of Structural Chemistry.