Yuan Q.-L.,Xi'an Jiaotong University |
Guo T.-M.,Xianyang Central Hospital |
Liu L.,Xi'an Jiaotong University |
Sun F.,Xi'an Jiaotong University |
And 2 more authors.
PLoS ONE | Year: 2015
Background: Neck pain (NP) and low back pain (LBP) are common symptoms bothering people in daily life. Traditional Chinese medicine (TCM) has been used to treat various symptoms and diseases in China and has been demonstrated to be effective. The objective of the present study was to review and analyze the existing data about pain and disability in TCM treatments for NP and LBP. Methods: Studies were identified by a comprehensive search of databases, such as MEDLINE, EMBASE, and Cochrane Library, up to September 1, 2013. A meta-analysis was performed to evaluate the efficacy and safety of TCM in managing NP and LBP. Results: Seventy five randomized controlled trials (n = 11077) were included. Almost all of the studies investigated individuals experiencing chronic NP (CNP) or chronic LBP (CLBP). We found moderate evidence that acupuncture was more effective than sham-acupuncture in reducing pain immediately post-treatment for CNP (visual analogue scale (VAS) 10 cm, mean difference (MD) = -0.58 (-0.94, -0.22), 95%confidence interval, p = 0.01), CLBP (standardized mean difference = -0.47 (-0.77, -0.17), p = 0.003), and acute LBP (VAS 10 cm, MD = -0.99 (-1.24, -0.73), p< 0.001). Cupping could bemore effective than waitlist in VAS (100 mm) (MD = -19.10 (-27.61, -10.58), p < 0. 001) for CNP or medications (e.g. NSAID) for CLBP (MD = -5.4 (-8.9, -0.19), p = 0.003). No serious or life-threatening adverse effects were found. Conclusions: Acupuncture, acupressure, and cupping could be efficacious in treating the pain and disability associated with CNP or CLBP in the immediate term. Gua sha, tai chi, qigong, and Chinese manipulation showed fair effects, but we were unable to draw any definite conclusions, and further research is still needed. The efficacy of tuina and moxibustion is unknown because no direct evidence was obtained. These TCM modalities are relatively safe. © 2015 Yuan et al.
Li E.-C.,Xi'an Jiaotong University |
Wen C.-F.,XiAn Medical College
Journal of Medicine and Philosophy | Year: 2010
This essay explores a tragic event that happened in China, which garnered much attention, the Li case: a young woman who was nine months pregnant and her baby died as a result of the failure to receive a medically necessary c-section due to the hospital having failed to secure her family's consent for the c-section. Differing from some critiques, this essay argues that the Li case should not be used to blame the Confucian family-determination model that has been applied in Chinese society for thousands of years. Based on summarizing the reasons supporting the model, this essay indicates that it is an integral part of the model that, in emergency or special cases, the physician must take medical action to save the patient, without the need to secure the consent of a family member. In order to prevent tragic cases like the Li case from happening, we recommend that relevant Chinese laws be further developed and specified and that, most importantly, Chinese physicians must cultivate the Confucian virtue of benevolence in their practice of taking care of patients in a virtuous way, along with patients' families. © The Author 2010. Published by Oxford University Press, on behalf of the Journal of Medicine and Philosophy Inc. All rights reserved.
A reversed CD4/CD8 ratio of tumor-infiltrating lymphocytes and a high percentage of CD4+FOXP3+ regulatory T cells are significantly associated with clinical outcome in squamous cell carcinoma of the cervix
Shah W.,Xi'an Jiaotong University |
Shah W.,Khyber Medical University |
Yan X.,Xi'an Jiaotong University |
Jing L.,Ningxia Medical University |
And 3 more authors.
Cellular and Molecular Immunology | Year: 2011
In this study, 40 biopsy samples collected from cervical cancer patients at the First Affiliated Hospital of Xi'an Jiaotong University, China, were retrospectively assessed using immunohistochemistry for CD4+ and CD8+ tumor-infiltrating lymphocytes (TILs) and were analyzed for the expression of FOXP3, OX40, granzyme B (GrB) and perforin (Prf). The proliferating index of the TILs was determined by assessing Ki67 expression. We determined the prognostic value of low and high numbers of TILs on survival by performing Kaplan-Meier analysis using median values as the cut-off points. Except for the number of CD4+FOXP3+ regulatory T cells (Tregs) and the CD4/CD8 ratio, none of the CD4+, CD8+, OX40+, GrB+ or Prf+ TILs were associated with the overall 5-year survival rate. The 5-year survival rate was significantly lower in patients who had a high percentage of Tregs as compared with the those who had a lower percentage (35.3% versus 88.9%, P=0.001), while the 5-year survival rate was significantly higher in patients with a high CD4/CD8 ratio as compared with patients who had a low CD4/CD8 ratio (82.4% versus 44.4%, P=0.029). When we considered the deaths and surviving cases as separate groups, we found that both the number of CD4+ T cells and the CD4/CD8 ratio were significantly lower in patients who died as compared with those who survived (26.33±11.80 versus 47.79±38.18, P=0.023 and 0.60±0.25 versus 1.17±1.02, P=0.019, respectively). In conclusion, decreased proportions of tumor-infiltrating CD4+ T cells with high percentages of Tregs and reversed CD4/CD8 ratios were significantly associated with the clinical outcome of patients with cervical carcinoma. © 2011 CSI and USTC. All rights reserved.
Wang L.-Q.,Fourth Hospital of xiAn |
Yan C.-F.,First Peoples Hospital of Baiyin City Gansu |
Zhao Y.,Xi'an Jiaotong University |
Chu J.,Fourth Hospital of xiAn |
Yu X.-W.,XiAn Medical College
Acta Obstetricia et Gynecologica Scandinavica | Year: 2014
Objective To examine the expression of CD200 and its receptor (CD200R) in human chorionic villi during the first trimester of normal pregnancy and early spontaneous abortion (ESA). Design Prospective study.Methods Expression of CD200 and CD200R in the chorionic villi was determined using streptavidin-peroxidase immunohistochemistry, confocal laser scanning microscopy and real-time polymerase chain reaction. © 2014 Nordic Federation of Societies of Obstetrics and Gynecology.Population Thirty-five women diagnosed with ESA and 30 women experiencing a healthy pregnancy in a medical university hospital in China were enrolled in this study between 2011 and 2013.Main outcome measures CD200 and CD200R expression.Results The expression of CD200 in syncytiotrophoblast cells was significantly higher during normal pregnancy than in ESA (0.51 ± 0.05 vs. 0.35 ± 0.05). In contrast, expression of CD200 in cytotrophoblast cells and CD200R in stromal cells was significantly lower during normal pregnancy when compared with ESA (CD200: 0.16 ± 0.02 vs. 0.32 ± 0.03; CD200R: 0.19 ± 0.03 vs. 0.22 ± 0.02). In villi, the expression of both CD200 protein and CD200R transcripts were significantly higher in healthy first-trimester pregnancy than in ESA (CD200: 156.89 ± 105.65 vs. 37.51 ± 17.62).Conclusions There is an increase in inhibitory properties of human chorionic villi during normal pregnancy. The mechanism underlying ESA might be associated with enhanced expression of CD200 and CD200R in the trophoblast, leading to an upregulation of the immune response during the first trimester of pregnancy. © 2014 Nordic Federation of Societies of Obstetrics and Gynecology.
Zhimin G.,Xi'an Jiaotong University |
Noor H.,Xi'an Jiaotong University |
Jian-Bo Z.,Xi'an Jiaotong University |
Lin W.,Xi'an Jiaotong University |
Rajiv Kumar J.,Xian Medical College
Medical Science Monitor | Year: 2013
Hilar cholangiocarcinoma (HC) is a rare tumor that causes devastating disease. In the late stages, this carcinoma primarily invades the portal vein and metastasizes to the hepatic lobes; it is associated with a poor prognosis. HC is diagnosed by its clinical manifestation and results of imaging techniques such as ultrasound, computed tomography, magnetic resonance cholangiopancreatography, endoscopic retrograde cholangiography, and percutaneous transhepatic cholangiography. Preoperative hepatic bile drainage can improve symptoms associated with insufficient liver and kidney function, coagulopathy, and jaundice. Surgical margin-negative (R0) resection, including major liver resection, is the most effective and potentially curative treatment for HC. If the tumor is not resected, then liver transplantation with adjuvant management can improve survival. We conducted a systematic review of developments in imaging studies and major surgical hepatectomy for HC with positive outcomes regarding quality of life. © Med Sci Monit.
Shao L.,Xian Medical College
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2013
To isolate the side population (SP) and non-side population (NSP) cells from A431 cells and compare their difference in tumorigenicity in mice and the expression profiles of epithelial-mesenchymal transition (EMT) markers. A431 cells stained with Hoechst 33342 were sorted with flow cytometry. The isolated SP cells and NSP cells were inoculated into NOD/SCID mice and the tumorigenicity of the cells was observed. EMT markers E-cadherin, β-catenin, vimentin, AXL, and Erbb3 in the tumor tissues were detected by immunohisto-staining. The tumors generated by SP cells were larger than those by NSP cells in NOD/SCID mice. Compared with the tumors generated by NSP cells, the cells in the periphery of tumors generated by SP cells showed up-regulated expressions of AXL, vimentin and β-catenin and down-regulated ERBB3 and E-cadherin. The SP cells in A431 cells have a strong tumorigenicity and show more EMT phenotypes in tissues.
Chu W.W.,Xian Medical College
Sheng li xue bao : [Acta physiologica Sinica] | Year: 2010
The present study aimed to investigate the change of cytochrome c in postconditioning-attenuated ischemia-reperfusion (I/R)-induced mucosal apoptosis in rat intestine compared with ischemic preconditioning (IPC). Using rat model of intestine I/R injury, male Sprague-Dawley rats weighing 220-250 g were divided into 4 groups which were Sham operation group, I/R group, IPC group and ischemic postconditioning (IPOST) group. In these groups, I/R procedure was performed by the occlusion of the superior mesenteric artery (SMA) for 45 min followed by reperfusion for 1 h. In Sham group, there was no intervention. In IPC group, SMA was occluded for 5 min and reperfused for 5 min, for two cycles, before the prolonged occlusion. In IPOST group, three cycles of 30-s reperfusion and 30-s reocclusion were preceded at the start of reperfusion. After the reperfusion, the small intestines were sampled for experimental detection. Intestinal mucosal mitochondrial membrane potential was detected by confocal laser scanning microscopy. Expressions of cytochrome c and caspase-3 proteins were detected using Western-blot method. The apoptosis of intestinal mucosal cells was determined with agarose gel electrophoresis and deoxynucleotidyl transferase mediated dUTP-biotin nick-end labeling (TUNEL) technique. Compared with I/R group, the mitochondrial membrane potentials and the expressions of cytochrome c protein were significantly increased, while the expressions of caspase-3 and the apoptotic rates were decreased in IPOST and IPC groups (P<0.05). There were no significant differences between IPOST and IPC groups (P>0.05). These data provide substantial evidence that IPOST attenuates I/R-induced mucosal apoptosis by reducing the release of cytochrome c from mitochondria in the rat small intestine.
Li H.Y.,Xian Medical College
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2012
To investigate the effects of arsenic trioxide (As(2);O(3);) on human hepatocellular carcinoma SMMC-7721 cells' migration and invasion and the underlying molecular mechanism. The effects of As(2);O(3); on cell migration and invasion were examined by wound-healing and Transwell assays; The expressions of CD147 and MMP-2 at both mRNA and protein levels in SMMC-7721 cells treated with As(2);O(3); were determined by real-time PCR and Western blotting, respectively. The wound-healing assay showed that the scuffing distance of SMMC-7721 cells exposed to 2.0 μmol/L As(2);O(3); for 12 and 24 h was significantly wider as compared with the ones unexposed to As(2);O(3);, indicating As(2);O(3); could inhibit the migration in SMMC-7721 cells (P<0.05). The Transwel1 assay showed that the number of SMMC-7721 cells migrating through the Matrigel treated by 2.0 μmol/L As(2);O(3); for 12 and 24 h obviously decreased as compared with non-treated ones, indicating that As(2);O(3); could inhibit the invasion of SMMC-7721 cells (P<0.05). Real-time PCR showed that the expressions of CD147 and MMP-2 mRNA were down-regulated in SMMC-7721 cells treated by 2.0 μmol/L As(2);O(3); for 6, 12 and 24 h, respectively (P<0.05). Western blotting demonstrated that the expressions of CD147 and MMP-2 proteins decreased in SMMC-7721 cells exposed to 2.0 μmol/L As(2);O(3); for 12, 24 and 48 h, respectively (P<0.05). As(2);O(3); could inhibit the migration and invasion in human hepatocellular carcinoma SMMC-7721 cells, which may be related to the down-regulation of CD147 and MMP-2.
Jing H.,Xian Medical College
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2010
To study the technology for separation and purification of phenylethanoid glycosides from leaves of Forsythia suspensa by macroporous adsorption resin. The absorption and separation abilities of 10 kinds of adsorption resin were studied and the separation and purification technological process of phenylethanoid glycosides from leaves of Forsythia suspensa was investigated by HPLC with the content of Forsythoside A as an index. The optimal conditions were as follows: taking AB-8 resin column as adsorbent, the resin was washed by 20BV distilled water to remove impurity and 8BV 30% ethanol to elute phenylethanoid glycosides, the eluting velocity was 2BV/h, the content of Forsythoside A in the extract was 34.8%. The process with AB-8 resin is an effective method to separate and purify phenylethanoid glycosides.
Wang L.,XiAn Medical College
Chinese Journal of Endemiology | Year: 2013
Objective: To study the relationship between extraocular muscle thickness and exophthalmos degree in patients with thyroid-associated ophthalmopathy(TAO), so as to provide a scientific basis for guiding clinical treatment. Methods: Forty seven patients diagnosed with TAO from May 2005 to May 2011, in the 451 Hospital of the People's Liberation Army were chosen in this study, and divided into TAO group(23 cases) and treatment group (24 cases). Meanwhile, another 32 healthy people were selected as control. Extraocular rectus muscles (including the medial rectus muscle, lateral rectus muscle, superior rectus muscle and inferior rectus muscle) of all participants were measured with B-scan ultrasonography, and exophthalmos degree with Hertel exophthalmometer. Results: The thicknesses of medial rectus in control group, TAO group and treatment group were (3.35 ± 0.25), (4.38 ± 0.85), (3.58 ± 0.52)mm, respectively; the thicknesses of lateral rectus muscle were (2.95 ± 0.61), (4.24 ± 0.75), (3.65 ± 0.42)mm, respectively; the thicknesses of superior rectus muscle were (3.15 ± 0.20), (4.65 ± 0.68), (4.01 ± 0.15)mm, respectively; the thicknesses of inferior rectus muscle were (3.17 ± 0.72),(4.75 ± 0.52),(3.71 ± 0.91)mm, respectively; the sums of binocular rectus thickness in these three groups were (21.95 ± 0.65), (28.02 ± 1.51), (22.95 ± 0.65)mm, respectively; the difference between the three groups was statistically significant (F= 2.32, 2.43, 2.25, 2.17, 5.63, all P < 0.05), and TAO group was significantly higher than that in the control group and in the treatment group (all P < 0.05), while there was no statistically significant between the control group and the treatment group(all P > 0.05). The eyes exophthalmos degree in TAO group and treatment group were (16.82 ± 2.46), (14.52 ± 1.83)mm, respectively. The sum of the rectus muscle thickness and eyes exophthalmos was positively correlated (r = 0.65, 0.58, all P < 0.05). Conclusions: Extraocular muscle thickness in patients with TAO is positively correlated with exophthalmos degree, and using B-scan ultrasonography to detect the changes is practical for clinical diagnosis and treatment.