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Xian, China

Li H.Y.,Xian Medical College
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2012

To investigate the effects of arsenic trioxide (As(2);O(3);) on human hepatocellular carcinoma SMMC-7721 cells' migration and invasion and the underlying molecular mechanism. The effects of As(2);O(3); on cell migration and invasion were examined by wound-healing and Transwell assays; The expressions of CD147 and MMP-2 at both mRNA and protein levels in SMMC-7721 cells treated with As(2);O(3); were determined by real-time PCR and Western blotting, respectively. The wound-healing assay showed that the scuffing distance of SMMC-7721 cells exposed to 2.0 μmol/L As(2);O(3); for 12 and 24 h was significantly wider as compared with the ones unexposed to As(2);O(3);, indicating As(2);O(3); could inhibit the migration in SMMC-7721 cells (P<0.05). The Transwel1 assay showed that the number of SMMC-7721 cells migrating through the Matrigel treated by 2.0 μmol/L As(2);O(3); for 12 and 24 h obviously decreased as compared with non-treated ones, indicating that As(2);O(3); could inhibit the invasion of SMMC-7721 cells (P<0.05). Real-time PCR showed that the expressions of CD147 and MMP-2 mRNA were down-regulated in SMMC-7721 cells treated by 2.0 μmol/L As(2);O(3); for 6, 12 and 24 h, respectively (P<0.05). Western blotting demonstrated that the expressions of CD147 and MMP-2 proteins decreased in SMMC-7721 cells exposed to 2.0 μmol/L As(2);O(3); for 12, 24 and 48 h, respectively (P<0.05). As(2);O(3); could inhibit the migration and invasion in human hepatocellular carcinoma SMMC-7721 cells, which may be related to the down-regulation of CD147 and MMP-2. Source

Shao L.,Xian Medical College
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2013

To isolate the side population (SP) and non-side population (NSP) cells from A431 cells and compare their difference in tumorigenicity in mice and the expression profiles of epithelial-mesenchymal transition (EMT) markers. A431 cells stained with Hoechst 33342 were sorted with flow cytometry. The isolated SP cells and NSP cells were inoculated into NOD/SCID mice and the tumorigenicity of the cells was observed. EMT markers E-cadherin, β-catenin, vimentin, AXL, and Erbb3 in the tumor tissues were detected by immunohisto-staining. The tumors generated by SP cells were larger than those by NSP cells in NOD/SCID mice. Compared with the tumors generated by NSP cells, the cells in the periphery of tumors generated by SP cells showed up-regulated expressions of AXL, vimentin and β-catenin and down-regulated ERBB3 and E-cadherin. The SP cells in A431 cells have a strong tumorigenicity and show more EMT phenotypes in tissues. Source

Chu W.W.,Xian Medical College
Sheng li xue bao : [Acta physiologica Sinica] | Year: 2010

The present study aimed to investigate the change of cytochrome c in postconditioning-attenuated ischemia-reperfusion (I/R)-induced mucosal apoptosis in rat intestine compared with ischemic preconditioning (IPC). Using rat model of intestine I/R injury, male Sprague-Dawley rats weighing 220-250 g were divided into 4 groups which were Sham operation group, I/R group, IPC group and ischemic postconditioning (IPOST) group. In these groups, I/R procedure was performed by the occlusion of the superior mesenteric artery (SMA) for 45 min followed by reperfusion for 1 h. In Sham group, there was no intervention. In IPC group, SMA was occluded for 5 min and reperfused for 5 min, for two cycles, before the prolonged occlusion. In IPOST group, three cycles of 30-s reperfusion and 30-s reocclusion were preceded at the start of reperfusion. After the reperfusion, the small intestines were sampled for experimental detection. Intestinal mucosal mitochondrial membrane potential was detected by confocal laser scanning microscopy. Expressions of cytochrome c and caspase-3 proteins were detected using Western-blot method. The apoptosis of intestinal mucosal cells was determined with agarose gel electrophoresis and deoxynucleotidyl transferase mediated dUTP-biotin nick-end labeling (TUNEL) technique. Compared with I/R group, the mitochondrial membrane potentials and the expressions of cytochrome c protein were significantly increased, while the expressions of caspase-3 and the apoptotic rates were decreased in IPOST and IPC groups (P<0.05). There were no significant differences between IPOST and IPC groups (P>0.05). These data provide substantial evidence that IPOST attenuates I/R-induced mucosal apoptosis by reducing the release of cytochrome c from mitochondria in the rat small intestine. Source

Shah W.,Xian Jiaotong University | Shah W.,Khyber Medical University | Yan X.,Xian Jiaotong University | Jing L.,Ningxia Medical University | And 3 more authors.
Cellular and Molecular Immunology | Year: 2011

In this study, 40 biopsy samples collected from cervical cancer patients at the First Affiliated Hospital of Xi'an Jiaotong University, China, were retrospectively assessed using immunohistochemistry for CD4+ and CD8+ tumor-infiltrating lymphocytes (TILs) and were analyzed for the expression of FOXP3, OX40, granzyme B (GrB) and perforin (Prf). The proliferating index of the TILs was determined by assessing Ki67 expression. We determined the prognostic value of low and high numbers of TILs on survival by performing Kaplan-Meier analysis using median values as the cut-off points. Except for the number of CD4+FOXP3+ regulatory T cells (Tregs) and the CD4/CD8 ratio, none of the CD4+, CD8+, OX40+, GrB+ or Prf+ TILs were associated with the overall 5-year survival rate. The 5-year survival rate was significantly lower in patients who had a high percentage of Tregs as compared with the those who had a lower percentage (35.3% versus 88.9%, P=0.001), while the 5-year survival rate was significantly higher in patients with a high CD4/CD8 ratio as compared with patients who had a low CD4/CD8 ratio (82.4% versus 44.4%, P=0.029). When we considered the deaths and surviving cases as separate groups, we found that both the number of CD4+ T cells and the CD4/CD8 ratio were significantly lower in patients who died as compared with those who survived (26.33±11.80 versus 47.79±38.18, P=0.023 and 0.60±0.25 versus 1.17±1.02, P=0.019, respectively). In conclusion, decreased proportions of tumor-infiltrating CD4+ T cells with high percentages of Tregs and reversed CD4/CD8 ratios were significantly associated with the clinical outcome of patients with cervical carcinoma. © 2011 CSI and USTC. All rights reserved. Source

Li E.-C.,Xian Jiaotong University | Wen C.-F.,Xian Medical College
Journal of Medicine and Philosophy | Year: 2010

This essay explores a tragic event that happened in China, which garnered much attention, the Li case: a young woman who was nine months pregnant and her baby died as a result of the failure to receive a medically necessary c-section due to the hospital having failed to secure her family's consent for the c-section. Differing from some critiques, this essay argues that the Li case should not be used to blame the Confucian family-determination model that has been applied in Chinese society for thousands of years. Based on summarizing the reasons supporting the model, this essay indicates that it is an integral part of the model that, in emergency or special cases, the physician must take medical action to save the patient, without the need to secure the consent of a family member. In order to prevent tragic cases like the Li case from happening, we recommend that relevant Chinese laws be further developed and specified and that, most importantly, Chinese physicians must cultivate the Confucian virtue of benevolence in their practice of taking care of patients in a virtuous way, along with patients' families. © The Author 2010. Published by Oxford University Press, on behalf of the Journal of Medicine and Philosophy Inc. All rights reserved. Source

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