Yin X.-F.,Peking University |
Wang T.-B.,Peking University |
Zhang P.-X.,Peking University |
Kou Y.-H.,Peking University |
And 21 more authors.
Chinese Medical Journal
Background: This study aimed to evaluate the effects of standard rescue procedure (SRP) in improving severe trauma treatments in China. Methods: This study was conducted in 12 hospitals located in geographically and industrially different cities in China. A standard procedure on severe trauma rescue was established as a general rule for staff training and patient treatment. A regional network (system) efficiently integrating prehospital rescue, emergency room treatments, and hospital specialist treatments was built under the rule for information sharing and improving severe trauma treatments. Treatment outcomes were compared between before and 1 year after the implementation of the SRP. Results: The outcomes of a total of 74,615 and 12,051 trauma cases were collected from 12 hospitals before and after the implementation of the SRP. Implementation of the SRP led to efficient cooperation and information sharing of different treatment services. The emergency response time, prehospital transit time, emergency rescue time, consultation call time, and mortality rate of patients were 24.24 ± 4.32 min, 45.69 ± 3.89 min, 6.38 ± 1.05 min, 17.53 ± 0.72 min, and 33.82% ± 3.87% (n = 441), respectively, before the implementation of the standardization and significantly reduced to 10.11 ± 3.21 min, 22.39 ± 4.32 min, 3.26 ± 0.89 min, 3.45 ± 0.45 min, and 20.49% ± 3.11%, separately (n = 495, P < 0.05) after that. Conclusions: Staff training and SRP can significantly improve the efficiency of severe trauma treatments in China. © 2015 Chinese Medical Association. All rights reserved. Source
Sun Z.-M.,Peoples Hospital of Gansu Province |
Ling M.,Peoples Hospital of Gansu Province |
Feng W.-L.,Xian Honghui Hospital |
Dong X.-H.,Peoples Hospital of Gansu Province |
And 2 more authors.
Chinese Journal of Tissue Engineering Research
Background: Intermittent negative pressure has been proven to promote the repairing of soft tissue and bone healing, but the effect of negative pressure on the tendon-bone healing after anterior cruciate ligament reconstruction has not been reported. Objective: To research the effect of intermittent negative pressure on tendon-bone healing after anterior cruciate ligament reconstruction and on the biomechanics of tendon grafts. Methods: A total of 24 New Zealand white rabbits were randomly selected to establish the models of anterior cruciate ligament reconstruction of autogenous semitendinosus. The hind leg of one side was selected randomly as the negative pressure group, and the joint of the negative pressure side was connected with the micro-negative pressure aspirator through drainage tube and maintained a low-intensity and intermittent negative pressure; the contralateral hind leg was as the control and connected with ordinary drainage tube. Drainage tubes of both sides were removed at the same time after 5 days. At 6 weeks after modeling, the joint fluid was drawn to detect the expression levels of interleukin-1β; femur-ligament-tibia complex was used for tension measurement of tendon graft, and histological observation of tendon-bone interface. Results and Conclusion: One rabbit had joint infection, and finally 23 rabbits were included in the study. Tension measurement results showed that maximum load for breakage in negative group pressure was significantly greater than that in the control group (P < 0.05). Histological observation found that the number of osteoblasts in the negative pressure group was significantly more than that in the control group, and the difference was statistically significant (P < 0.01). Detection of synovial fluid showed that iterleukin-1β content in synovial fluid of the negative pressure group was lower than that in the control group, and the difference was statistically significant (P < 0.01). Intermittent negative pressure may play an active role in tendon-bone healing and modeling of tendon graft after anterior cruciate ligament reconstruction. Source
Jiang C.,Xian Jiaotong University |
Yu H.,Xian Children Hospital |
Sun Q.,Xian Jiaotong University |
Zhu W.,Xian Jiaotong University |
And 8 more authors.
BMC Pulmonary Medicine
Background: This study aims to find out whether extracellular miRNAs is implicated in recurrent childhood wheezing with asthmatic risk. Methods: One hundred and forty children of Chinese Han population were recruited for this study. Plasma and intracellular miRNAs from children with recurrent wheezing and rats with antigen induced pulmonary inflammation (AIPI) were detected by using reverse transcription-quantitative PCR. Differential leukocytes in blood were automatically counted. Total IgE was detected by enzyme-linked immunosorbent assay. Clinical implication in diagnosis was evaluated using receiver operating characteristic curves. Results: The increase of plasma miR-21 and miR-26a was screened out from 11 candidate miRNAs and validated in wheezing children. The level of expression for both miRNAs were comparable in different age and gender. Plasma miR-21 was more preferable to miR-26a and total IgE for diagnosis. Plasma miR-21 and miR-26a levels were not significantly correlated with various leukocyte counts or miRNA expression in blood cells. In acute and chronic AIPI rats, miR-21 levels increased in both plasma and lavaged lung compared with control. Moreover, circulating miR-21 and miR-26a levels were highly positively correlated with infiltrated cell counts in bronchoalveolar lavage fluid of AIPI rats. Conclusions: Circulating miR-21 and miR-26a increase in wheezing children and AIPI rats. This not only manifests their strong clinical implication in recurrent childhood wheezing with asthma risk, but also provides novel insights into the role of extracellular miRNAs during development of airway inflammation and recurrent wheezing. © 2016 Jiang et al. Source
Zhou Y.,Xian Jiaotong University |
Mou K.-H.,Xian Jiaotong University |
Han D.,Xian Jiaotong University |
Li Y.,Xian Jiaotong University |
And 3 more authors.
Journal of Xi'an Jiaotong University (Medical Sciences)
Objective: To investigate the effects of matrine (Mat) on the expressions of IL-17RA, IL-21R and IL-22R1 induced by Th17 cytokines in HaCaT of keratinocytes.Methods: We cultured HaCaT cells and stimulated HaCaT cells with Th17 effector cytokines IL-17A (10 ng/mL), IL-21 (10 ng/mL) and IL-22 (10 ng/mL) together to simulate psoriasis-like cell model. The cell model was treated with 10 μg/mL and 100 μg/mL Mat. RT-qPCR and Western blot were applied to detect the expressions of IL-17RA, IL-21R and IL-22R1.Methods: IL-17RA, IL-21R and IL-22R1 were expressed in HaCaT cells at both mRNA and protein levels. HaCaT cells triggered by IL-17A, IL-21 and IL-22 together could significantly enhance the mRNA and protein expressions of IL-17RA and IL-21R as well as the protein expression of IL-22R1 (P<0.05). Mat treatment on the cells without Th17 cytokine stimulation did not affect IL-17RA, IL-21R or IL-22R1 expression (P>0.05), but Mat treatment to the cells separately or with Th17 cytokine stimulation together can significantly inhibit IL-17RA, IL-21R and IL-22R1 protein expressions compared with Th17 cytokine stimulation group (P<0.05).Conclusion: Stimulation of HaCaT cells with IL-17A, IL-21 and IL-22 can enhance the expressions of IL-17RA, IL-21R and IL-22R1. Mat seems to play an anti-inflammatory role through abrogating the stimulatory effects of Th17 cytokines on their receptor expressions in autoimmune skin disease. Source
Jiang C.,Health Science University |
Jiang C.,Key Laboratory Of Environment And Genes Related To Diseases Xian Jiaotong University |
Meng L.,Health Science University |
Meng L.,Key Laboratory Of Environment And Genes Related To Diseases Xian Jiaotong University |
And 9 more authors.
International Journal of Clinical and Experimental Medicine
Introduction: Extracellular miRNAs in the blood plasma/serum of patients as novel promising diagnostic markers for various diseases has aroused huge attention. Among various major platforms for extracellular miRNA detection, extensively used RT-qPCR is considered practical and cost-efficient in experimental investigation. However, there are also many technical challenges, hence the development of robust methodology for extracellular miRNA detection is in good need. Methods: Focused on the controversial procedures present in recent studies, we intend to solving these technical problems and seeking for optimal procedures for extracellular miRNA profiling using the RT-qPCR method. Results: We addressed our concerns during the whole detection such as various key points during blood handling, choice making during RNA isolation and miRNA RT process, practical management in global miRNAs profiling using PCR as well as data normalization and processing. Meanwhile, we described and evaluated our profiling method in detail, intended to optimize the procedures that might lead to variable results from independent experiments, and further recommend this standardized optimal procedure. Conclusion: Our work might provide a potent technical optimization for extracellular miRNA detection. © 2016, E-Century Publishing Corporation. All rights reserved. Source