Xian GaoXin Hospital
Xian GaoXin Hospital
Cheng H.-W.,Xintai Peoples Hospital |
He J.-G.,Xian Gaoxin Hospital |
He W.-Y.,Yanan Peoples Hospital
Saudi Journal of Biological Sciences | Year: 2017
The main objective of the present research work was to evaluate the antitumor effects of ethanol extract of Semen Euphorbiae (EESE) in ACHN human renal carcinoma cells. The effects on apoptosis induction, cell cycle phase distribution and livin protein expression were also evaluated. MTT assay was used to assess cytotoxic effects of the extract while as clonogenic assay was used to evaluate effects on colony formation tendency. Inverted phase contrast and fluorescence microscopic techniques were used to evaluate effects of EESE on cellular morphology and apoptosis. Flow cytometry using annexin V-FITC and propidium iodide were used to quantify the extent of apoptosis and also to evaluate effects on cell cycle. Results indicate that ethanol extract of Semen Euphorbiae exhibited potent cytotoxic effects in ACHN human renal cancer cells. These effects were found to be dose-dependent as well as time dependent. Clonogenic assay revealed that EESE led to dose-dependent inhibition of colony formation in these cells. EESE-treated cells also showed evident signs of alterations and deformations in cell morphology including detachment of cells from one another forming small cluster of cells. In contrast to untreated control cells, EESE-treated cells with 10, 100 and 200. μg/ml dose showed an increase in the number of cells emitting red/orange fluorescence indicating onset and execution of apoptosis. EESE extract also led to G2/M cell cycle arrest in these cells. © 2017 The Authors.
Wu X.,Xi'an Jiaotong University |
Zhou J.,Xi'an Jiaotong University |
Zhou J.,XiAn Gaoxin Hospital |
Cai D.,Xi'an Jiaotong University |
Li M.,Xi'an Jiaotong University
Oncology Reports | Year: 2017
Matrine is a traditional Chinese herbal medicine that shows antitumor efficacy for many types of cancer. The present study evaluated the antitumor efficacy of matrine on cervical cancer and to investigate the underlying mechanisms. We performed MTT assays, and in vitro invasion and migration assays, and P1 L6 found that matrine significantly inhibited cervical cancer cell growth by inducing apoptosis, and suppressed the invasion and migration ability of cervical cancer cells in vitro in a concentration-dependent manner. Mechanistically, we found that matrine decreased the expression and activity of the extracellular matrix factors, matrix metalloproteinases-2 (MMP-2) and MMP-9 via the suppression of p38 signaling pathway. In addition, when cervical cancer cells were grown as xenografts in nude mice, intraperitoneal (i.p.) injection of matrine induced a significant dose-dependent decrease in tumor growth. Taken together, these findings suggest that a potential mechanism by which matrine inhibits the growth and metastasis of cervical cancer through downregulating the p38 signaling pathway.
Jin C.,PLA Fourth Military Medical University |
Li H.,PLA Fourth Military Medical University |
He Y.,PLA Fourth Military Medical University |
He M.,Qinshui Prefectural Peoples Hospital |
And 4 more authors.
Journal of Cancer Research and Clinical Oncology | Year: 2010
Purpose Systemic combination chemotherapy is the only option for patients with unresectable hepatocellular carcinoma (HCC) not suitable for intra-arterial treatment. However, no systemic chemotherapy has been able to provide durable remission. The search for a new combination of drugs for HCC is significant. The combination of doxorubicin and paclitaxel shows promise in breast cancer therapy. This study was carried out to determine the synergistic effect of combined doxorubicin and paclitaxel in the two HCC cell lines: HepG2 and Huh7 in vitro and murine HCC H22-bearing BALB/c mice in vivo. Methods The morphology of the two cell lines treated with drugs was photomicrographed. The 3-(4, 5-dimethylthiazol- 2-yl)-2, 5 diphenyltetrazolium bromide assay was used to determine the number of surviving cells. Cell cycle was evaluated by flow cytometry. Cell viability was measured by the ability of single cells to form colonies in vitro. Antitumor activities against subcutaneoulsy implanted solid tumor induced by H22 cells in mice were evaluated. Results Our data demonstrated that the cytotoxicity produced by doxorubicin and paclitaxel was additive in HCC cells, while it was mainly held in the G2/M phase of the cell cycle by paclitaxel. In vivo anti-tumor activity assay also showed that the combination of the two drugs resulted in more significant tumor regression, compared to the single one. Conclusion The study may provide a new combination of cytotoxic drugs for HCC chemotherapy. © Springer-Verlag 2009.
Su X.-L.,Xian Medical University |
Zhang H.,Shaanxi Provincial Peoples Hospital |
Yu W.,Xian Medical University |
Wang S.,Xian Medical University |
Zhu W.-J.,Xian GaoXin Hospital
Chinese Journal of Physiology | Year: 2013
Proliferation and migration of vascular smooth muscle cells (VSMCs) are important events in the development of diabetic atherosclerosis. Previous studies have suggested that KCa3.1 channels participate in atherosclerosis and coronary artery restenosis. In the present study, we attempted to clarify the roles of KCa3.1 channels in the proliferation and migration of VSMCs using experimental type-2 diabetes rat serum and aortic smooth muscle cells (SMC) prepared from non-diabetic rats. mRNA and protein levels and current density of KCa3.1 channels were greatly enhanced in cultured VSMCs treated with diabetic serum. In addition, diabetic serum promoted cell proliferation and migration in cultured VSMCs, and the effects were fully reversed in the cells treated with the KCa3.1 channels blocker TRAM-34. In conclusion, serum from diabetic rats increases the expression of KCa3.1 channels and promotes proliferation and migration of VSMCs to possibly participate in vascular remodeling in diabetes. © 2013 by The Chinese Physiological Society and Airiti Press Inc.
PubMed | Xi'an Jiaotong University, Xian Gaoxin Hospital, Lanzhou University, PLA Fourth Military Medical University and 2 more.
Type: | Journal: Epilepsy & behavior : E&B | Year: 2016
The objective of this study was to assess the electroclinical aspects and treatment of Han patients with juvenile myoclonic epilepsy (JME) in northern China.One hundred fifty-six outpatients with JME from six epilepsy centers, between January 2011 and June 2012, were followed up for at least two years. They underwent twenty-four-hour video-EEG recording. Brain imaging was performed using magnetic resonance imaging (MRI). Clinical aspects, electroencephalographic (EEG) features, and antiepileptic drugs (AEDs) received were reviewed.Generalized tonic-clonic seizures (GTCS) were found in 150/156 patients. Delay of diagnosis was 4.609.92years. Photosensitivity was more common in eye closure condition during IPS in patients with JME; in addition, patients with JME with myoclonic seizures (MS) and GTCS as seizure types were likely to present photoparoxysmal responses (PPRs). The 82 nontreated patients showed a median latency to first interictal or ictal generalized spike-wave discharge (GSWD) of 50min (IQR: 22-102min). The first GSWDs were recorded in 63%, 76%, 90%, and 98% patients within one, two, three, and 4h, respectively; only 2% of patients had first GSWDs after 4h. One hundred eleven patients (111/156) chose extended-release valproate (VPA) at daily doses 1000mg. The percentages of seizure-free patients among MS, GTCS, and absence seizure (AS) groups were 88.3%, 99.0%, and 94.9%, respectively.Photoparoxysmal responses were more common in patients with JME with MS and GTCS and rare in patients with JME with MS and AS in northern Chinese Han patients. Most patients with JME in northern China chose VPA as first therapeutic choice, and low dose (500 to 1000mg daily) of extended-release VPA may be an optimal choice for them. Video-EEG monitoring for at least 4h may be helpful in detecting the first interictal or ictal GSWD in patients with potential JME. Moreover, video-EEG monitoring performed at about 9 oclock in the morning with patients in the awake state might be useful to find the first GSWD. For JME diagnosis, Class II criteria are more helpful than Class I counterparts, the latter yielding more missed diagnoses.
Jin C.,PLA Fourth Military Medical University |
Qian N.,PLA Fourth Military Medical University |
Qian N.,Chinese People's Liberation Army |
Zhao W.,PLA Fourth Military Medical University |
And 6 more authors.
Biomacromolecules | Year: 2010
Although surgery offers the only hope of cure for hepatocellular carcinoma (HCC), patients with inoperable or metastatic disease have a dismal prognosis. Therefore, there is an urgent need for new and effective treatment strategies. Polymeric micelles composed of drug conjugated to a diblock copolymer have attracted great interest for clinical administration of anticancer drugs. In this work, characteristics and cytotoxicity of doxorubicin-poly(d,l-lactic-co- glycolic acid)-poly(ethylene glycol) (DOX-PLGA-PEG) micelle and its targeted micelle decorated with bivalent fragment HAb18 F(ab′)2 for HCC were studied. These micelles possessed spherical morphology and higher loading efficiency. Cellular uptake and accumulation in tumor tissue of micelles was observed. The accumulation of the targeted micelles depends on dual effects of passive and active targeting. The drug-loading micelles showed cytotoxicity on tumor cells in vitro and in vivo. The targeted micelles resulted in significant improvement in therapeutic response. These results suggest that the novel micelles could be a promising candidate with excellent therapeutic efficacy for targeting the drugs to cancer cells. © 2010 American Chemical Society.
Kong L.-J.,Xian Gaoxin Hospital
International Journal of Ophthalmology | Year: 2011
AIM: To observe the efficacy and safety of manual small incision cataract surgery (MSICS) in patients from the Republic of the Sudan. METHODS: Three hundreds patients(320 eyes) underwent MSICS with intraocular lens (IOL) implantation after excision of lens nucleus through tunnel incision. RESULTS: At the first day after operation, the uncorrected visual acuity (UCVA) was ≤0.1 in 16 eyes; 0.2-0.5 in 256 eyes; >0.5 in 48 eyes. IOL implantation was performed in 304 eyes (95.0%). CONCLUSION: MSICS is worth being spread because of rapid postoperative recovery, less complication.
Chen H.,Huazhong Agricultural University |
Jin X.,XiAn Gaoxin Hospital |
Li Y.,Huazhong Agricultural University |
Tian J.,Huazhong Agricultural University
RSC Advances | Year: 2016
The physical stability of a eugenol (Eu) nanoemulsion in the presence of long-chain and medium-chain triglycerides with a high incorporation ratio (30 wt% to 80 wt% in the lipid phase) was investigated. The stability of the nanoemulsion was evaluated by dynamic light scattering and a centrifugal-force-based LUMi-Sizer. With an increase in the content of triglycerides, the particle size of the nanoemulsions decreased first and then increased. The variation pattern of the particle size in the presence of different contents of triglycerides formed a V-type shape. The presence of 60 wt% triglycerides resulted in the smallest particle size, which continued increasing within 28 days of storage. LUMi-Sizer analyses demonstrated that the instability of the nanoemulsion caused by the addition of 30-50 wt% of triglyceride was due to sedimentation of emulsion droplets. On the other hand, the instability caused by 60-80 wt% of triglyceride was due to the floating of emulsion droplets. The interfacial tension variation had the same trend as the particle size variation at the corresponding content of triglycerides. Thus the interfacial tension might be responsible for the triglyceride-caused instability of the Eu nanoemulsion. © 2016 The Royal Society of Chemistry.
Zhou B.,Huazhong Agricultural University |
Jin X.,XiAn Gaoxin Hospital |
Li J.,Huazhong Agricultural University |
Xu W.,Huazhong Agricultural University |
And 3 more authors.
RSC Advances | Year: 2014
Layer-by-layer assembled films have been exploited for functional materials. Tannic acid with previously confirmed antibacterial and antioxidant potentials was deposited on cellulose nanofibrous mats. The LbL assembly technique allowed sufficient binding of TA and AgNPs-Lys to the supporting substrate via hydrogen bond and electrostatic interactions. The properties and morphology of the AgNPs-Lys/TA multilayer assembly membranes were characterized by X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectra (FT-IR), wide-angle X-ray diffraction (XRD), and scanning electron microscopy (SEM). The antibacterial and antioxidant activities were examined as well. The hybrid composite films have potential application in food packing and wound dressing, and tissue engineering, etc. This journal is © the Partner Organisations 2014.
Liu X.-G.,Xian Gaoxin Hospital |
Jiang H.-K.,Nuclear Industry 215 Hospital
Biotechnology Letters | Year: 2013
A double-chamber bioreactor has been developed to generate a tissue-engineered osteochondral composite (TEOC). However, a TEOC generally requires two types of cells (i.e. chondrogenic and osteogenic cells). Therefore, the capacity of mesenchymal stem cells (MSCs) as a single-cell source to work within a double-chamber bioreactor and biphasic scaffolds for generating TEOC was investigated. Compared with static culture, the double-chamber bioreactor not only can promote faster cellular proliferation, indicated by the PicoGreen dsDNA assay, SEM and confocal imaging, but also can trigger efficient chondrogenic and osteogenic differentiation of MSCs in biphasic scaffolds simultaneously, evidenced by gene expression. Thus MSCs are promising as the ideal single-cell source and the double-chamber bioreactor is an advanced culture system to generate TEOC. © 2013 Springer Science+Business Media Dordrecht.