Xiamen Yinxiang Group Co.

Xiamen, China

Xiamen Yinxiang Group Co.

Xiamen, China
SEARCH FILTERS
Time filter
Source Type

Shi L.,South China University of Technology | Shi L.,Xiamen Yinxiang Group Co. | Wang W.-Y.,South China University of Technology | Yan H.,South China University of Technology
Modern Food Science and Technology | Year: 2013

To investigate the pork contamination by Listeria monocytogenes, its susceptibility to antimicrobial agents and serotypes, all strains were isolated by the GB 4789.30-2010 method; L. monocytogenes serotypes were identified by PCR; and antibiotic resistant profile of the isolates was examined using disc diffusion assay. A total of 273 pork samples were examined, out of which 52 samples (19.02%) were positive for L. monocytogenes and 78 strains were detected. And 33 (42.31%), 24 (30.71%), 16 (20.51%), 2 (2.56%), 3 (3.85%) of the isolates were identified as serotypes 1/2a (or 3a), 1/2b (or 3b or 7), 1/2c (or 3c), 4a (or 4c) and 4b (or 4d or 4e), respectively. With disc diffusion assay, all strains were susceptible to the antibiotics, including ampicillin, ampicillin-sulbactam, imipenem, ciprofloxacin, levofloxacin, and vancomycin. However, the percentages of tetracycline resistances were higher. 11.54% isolates were resistant to penicillin, gentamicin, erythromycin. The conjugative transposon Tn916 is the important determinants of the resistance in spreading of the L. monocytogenes.


Shi L.,South China University of Technology | Shi L.,Xiamen Yinxiang Group CO. | Zhao Y.-M.,South China University of Technology | Zhao Y.-M.,Xiamen Yinxiang Group CO. | And 3 more authors.
Modern Food Science and Technology | Year: 2015

To evaluate the stability of enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) and Sau-PCR for typing of Listeria monocytogenes (LM), five isolates of LM from a local vegetable market in Guangzhou and a food processing plant in Xiamen were cultured. DNA was extracted from the cultures incubated at room temperature after 24, 48, and 72 h. In addition, DNA was extracted from the 5th, 10th, 15th, and 20th subcultures. ERIC-PCR and Sau-PCR were then used for typing and changes in fingerprint diagrams with time and increase in the number of generations in subculture were determined. The results indicated that apart from some missing bands, there were no additional bands after room-temperature culture and subculture. However, there were minor changes in the overall band appearance. The homology of ERIC-PCR and Sau-PCR results was higher than 92% and 94%, respectively, indicating that the two typing methods were stable and had epidemiological significance for typing of LM cultures incubated for less than 72 h and 20 generations. ©, 2015, South China University of Technology. All right reserved.


Li L.-L.,South China University of Technology | Shi L.,South China University of Technology | Shi L.,Xiamen Yinxiang Group CO. | He J.-H.,South China University of Technology | And 2 more authors.
Modern Food Science and Technology | Year: 2015

The mechanism of antibiotic resistance in foodborne, multidrug-resistant (MDR) Listeria monocytogenes was investigated to develop measures to control the MDR phenomenon. Genetic factors of antibiotic-resistance were screened by polymerase chain reaction (PCR), the position and dissemination capacity of these factors were evaluated by plasmid curing and natural transformation, and the stability of drug-resistance dissemination was verified by consecutive subculture. The results showed that the MDR strain LM78 (resistant to chloramphenicol, erythromycin, streptomycin, tetracycline, trimethoprim/sulfamethoxazole) carried the cat, ermB, and tetS genes. Following plasmid curing, the minimum inhibitory concentration (MIC) was reduced to the sensitive range. Moreover, the plasmid could be transferred between species by natural transformation. Plasmid curing and transformation results suggested that these resistance genes were encoded in the plasmid. After consecutive subculture without antibiotics, these plasmids remained sufficiently stable. The MDR trait of foodborne pathogens may be transferred between bacterial species, and subsequent infection in humans via contaminated foodposes a potential threat against human health. ©, 2015, South China University of Technology. All right reserved.

Loading Xiamen Yinxiang Group Co. collaborators
Loading Xiamen Yinxiang Group Co. collaborators