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Zeng X.,The Third Institute of Oceanography SOA | Zeng X.,Key Laboratory of Marine Genetic Resources of Fujian Province | Zeng X.,Xiamen State Key Laboratory Breeding Base of Marine Genetic Resources | Zhang Z.,The Third Institute of Oceanography SOA | And 17 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2015

A novel piezophilic, thermophilic, anaerobic, fermentative bacterial strain, designated strain DY22613T, was isolated from a deep-sea hydrothermal sulfide deposit at the East Pacific Rise (GPS position: 102.66 W 3.16 S). Cells of strain DY22613T were long, motile rods (10 to 20 mm in length and 0.5 mm in width) with peritrichous flagella and were Gram-stain-negative. Growth was recorded at 44–72 6C (optimum 60–62 6C) and at hydrostatic pressures of 0.1–55 MPa (optimum 20 MPa). The pHrange for growth was frompH 5.0 to 9.0 with an optimum at pH 7.0. Growth was observed in the presence of 1 to 8% (w/v) sea salts and 0.65 to 5.2% (w/v) NaCl, with optimum salt concentrations at 3.5% for sea salts and at 2.3% for NaCl. Under optimal growth conditions, the shortest generation time observed was 27 min (60 6C, 20 MPa). Strain DY22613T was heterotrophic, able to utilize complex organic compounds, amino acids, sugars and organic acids including peptone, tryptone, beef extract, yeast extract, alanine, glutamine, methionine, phenylalanine, serine, threonine, fructose, fucose, galactose, gentiobiose, glucose, mannose, melibiose, palatinose, rhamnose, turanose, pyruvate, lactic acid, methyl ester, erythritol, galacturonic acid and glucosaminic acid. Strain DY22613T was able to reduce Fe(III) compounds, including Fe(III) oxyhydroxide (pH 7.0), amorphous iron(III) oxide (pH 9.0), goethite (a-FeOOH, pH 12.0), Fe(III) citrate and elementary sulfur. Products of fermentation were butyrate, acetate and hydrogen. Main cellular fatty acids were iso-C15:0, iso-C14:0 3-OH and C14:0. The genomic DNA G+C content of strain DY22613T was 36.7 mol%. Based on 16S rRNA gene sequence analysis, the strain forms a novel lineage within the class Clostridia and clusters with the order Haloanaerobiales (86.92% 16S rRNA gene sequence similarity). The phylogenetic data suggest that the lineage represents at least a novel genus and species, for which the name Anoxybacter fermentans gen. nov., sp. nov. is proposed. The type strain is DY22613T (5JCM 19466T5DSM 28033T5MCCC 1A06456T). ©2015 IUMS.

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