Xiamen Ocean Vocational College

Xiamen, China

Xiamen Ocean Vocational College

Xiamen, China

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Chen H.,State Oceanic Administration | Chen H.,Xiamen University | Chen H.,Xiamen Ocean Vocational College | Wang M.,Xiamen Ocean Vocational College | And 3 more authors.
Food Microbiology | Year: 2017

As filter-feeding bivalves, oysters can accumulate microorganisms into their gills, causing spoilage and potential safety issues. This study aims to investigate the changes in the gill microbiota of oysters packed under air and modified atmospheres (MAs, 50% CO2: 50% N2, 70% CO2: 30% O2, and 50% CO2: 50% O2) during storage at 4 °C. The diversity of bacterial microbiota in oyster gills was profiled through polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis on the 16S rRNA gene V3 region to describe the variation during the entire storage period. The DGGE profile revealed high bacterial diversity in the air- and MA-packaged oyster gills, and the spoilage bacterial microbiota varied in the MA-packaged oyster gills. Results indicated that CO2:O2 (70%:30%) was suitable for oyster MA packaging and that high bacterial loads in oyster gills need to be considered during storage. In addition, Lactobacillus and Lactococcus species were found to grow dominantly in fresh oyster gills under MA packaging, which supports the potential application of MA packaging for oyster storage. © 2016 Elsevier Ltd


Pang J.,Fujian Agriculture and forestry University | Jian W.,Fujian Agriculture and forestry University | Jian W.,Xiamen Ocean Vocational College | Wang L.,Fujian Normal University | And 3 more authors.
Carbohydrate Polymers | Year: 2012

In order to improve the surface property of Konjac glucomannan (KGM) film, the nitrogen plasma modification was carried out using ion beam injection machine. The surface atomic composition was evaluated by X-ray photoelectron spectroscopy analysis afterward. It was observed that the atomic concentration ratio of wO/wC increased using plasma treatment. The molecular chain degraded and acetyl was partly removed. Hydroxyl was partly replaced by primary amide group. Generation of new functionality groups suggested that plasma treatment might be an effective means to modify the physical property of KGM film. © 2011 Elsevier Ltd All rights reserved.


PubMed | Xiamen Ocean Vocational College, South China Agricultural University, Shanghai Ocean University and Xiamen Medical College
Type: | Journal: Carbohydrate polymers | Year: 2016

Konjac glucomannan (KGM) is an important gelling agent in composite gels. This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP). In this work, TMP composite gels were prepared under neutral pH with varying KGM (native KGM, 10kGy-KGM, 20kGy-KGM, and 100kGy-KGM) of different molecular characteristics. Native KGM, 10kGy-KGM, and 20kGy-KGM exerted negative effect on gel strength or whiteness of TMP gels. Interestingly 100kGy-KGM improved gelling properties and whiteness of TMP gels. Such effects presented by varying KGM were attributed the physical filling behaviors and the interaction between KGM and TMP. These behaviors or interactions are resulted from different molecular size and conformation. Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure. Our study provided principle knowledge to understand the structure-functions relationships of KGM-TMP composite gels. These results can be used to provide theoretical guidance for surimi gel processing.


Lin L.,Xiamen Ocean Vocational College | Liuzhen,Xiamen University
Proceedings of the 9th International Conference on Computer Science and Education, ICCCSE 2014 | Year: 2014

Chip quality detection is one of the important procedures before the picking up operation of chip by mounter. Fast image matching method was proposed to conduct the quality inspection of micro-electromechanical systems (MEMS) chip. The advantages and disadvantages of gray projection based matching algorithm were analyzed. Then a compound projective feature matching method was put forward. VC based algorithm was given to achieve compound projective feature matching method. Compared with the traditional gray matching algorithm and the normalized cross-correlation algorithm, the ability to match and the match time of the proposed matching method were studied. Contrast with single direction of projection matching method, the matching accuracy of the given matching method was tested. Meanwhile the matching ability of proposed algorithm was test for a small angle rotation of image. Finally, the compound projection-based matching method was used to detect the quality of the MEMS chip, and the practicality of the proposed method in terms of time and accuracy were verified by experimental results. © 2014 IEEE.


Wang J.-Q.,Hebei University | Li L.-C.,Xiamen Ocean Vocational College | Lin S.-G.,Shenyang Pharmaceutical University | Wang Z.-P.,Weinan Normal University
Biochemical Genetics | Year: 2010

The p8 gene encodes a transcription factor known to modulate cell growth, division, and apoptosis and influences gene expression. In this study, an Atlantic halibut (Hippoglossus hippoglossus) homolog of the p8 gene was cloned, sequenced, and characterized. The full-length p8 cDNA consists of 601 bp and encodes 76 amino acids with a molecular mass of 9 kD. The bHLH region is well conserved between Atlantic halibut and other animals. Analysis by RT-PCR showed that the p8 transcript is constitutively expressed in 9 of the 12 tissues tested: pancreas, intestine, stomach, gill, head kidney, heart, liver, ovary, and spleen. A predicted microRNA target site was found in the 30UTR of Atlantic halibut p8 mRNA. We speculate that the target site may pair to microRNA molecules because the target site resides in a big loop, a space large enough for the binding of microRNA molecules. © Springer Science+Business Media, LLC 2010.


Jian W.,Fujian Agriculture and forestry University | Jian W.,Xiamen Ocean Vocational College | Zeng Y.,Fujian Agriculture and forestry University | Xiong H.,Fujian Agriculture and forestry University | Pang J.,Fujian Agriculture and forestry University
Carbohydrate Polymers | Year: 2011

The formation of konjac glucomannan-borate complex in water solution has been investigated by experimental and molecular simulation methods. The energy, radical distribution function of borate anion (B(OH) 4 -), and mean square displacement of the complex were studied during the molecular simulation, and the results indicate that one type of helical complex can be formed based on the hydrogen bonds between borate anion and helical chain of KGM. The hydrogen bond is formed by the interaction of borate anion and -OH groups on C (6) of mannose and glucose. Temperature has little effect on the helical conformation of the complex. It was proposed that the complex can form gel through the aggregation arrangement of helical chain. © 2011 Elsevier Ltd. All rights reserved.


Chen H.,Fujian Agriculture and forestry University | Chen H.,Xiamen Ocean Vocational College | Liu Z.,Fujian Agriculture and forestry University | Liu Z.,Fisheries Research Institute of Fujian | And 3 more authors.
Journal of the Science of Food and Agriculture | Year: 2013

Background: The spoilage bacterial community in oyster gill was investigated during storage at 4, 10 and 20°C. Aerobic plate counts and pH values were determined. Total bacterial DNA was extracted from oyster gill and bulk cells of plate count media. The major bacterial species during fresh or different temperatures storage were determined by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Results: The initial aerobic plate count in oyster gill reached 6.70 log CFU g-1. PCR-DGGE fingerprinting analysis of the 16S rRNA gene V3 region revealed that most of the strains in fresh oyster gill belonged to the genera Lactococcus and Enterobacter. The major spoilage bacteria at a storage temperature of 20°C were Leuconostoc pseudomesenteroides, an uncultured bacterium, Cytophaga fermentans, Lactococcus lactis, Pseudoalteromonas sp., Enterococcus mundtii, Clostridium difficile and an uncultured Fusobacteria; those at 10°C were Lactococcus spp., Lactobacillus curvatus, Weissella confusa and C. difficile; those at 4°C were Lactococcus, Weissella, Enterobacter and Aeromonas. The other minor species were L. curvatus, Pseudomonas sp. and E. mundtii. Lactococcus spp. was the most common main spoilage bacteria in oyster gill during chilled storage. Conclusion: PCR-DGGE revealed the complexity of the bacterial microbiota and the major bacteria species in oyster gill for fresh and storage. © 2013 Society of Chemical Industry.


Wu Y.,Xiamen Ocean Vocational College | Zhou S.,Inspection and Quarantine Technology Center | Xu D.,Inspection and Quarantine Technology Center
Chinese Journal of Chromatography (Se Pu) | Year: 2013

A method for the determination of histamine (HIS7, tryptamine (TRP), 2-phenyle-thylamine (2-PHE), putrescine (PUT), cadaverine (CAD), tyramine (TYR), spermidine (SPD), spermine (SPM) in animal-derived foodstuffs was established using high performance liquid chromatography-tandem mass spectrometry. The samples were extracted with acetoni-trile-formic acid aqueous solution, and purified with strong cationic exchange (MCX) cartridge. Without derivatization, the eight biogenic amines (BAs) were separated by hydrophilic interaction chromatographic (HILIC) column with the mobile phases of 5 mmol/L ammonium acetate and methanol. The linearities were excellent in the range of 0. 001-100 μg/L with the correlation coefficients above 0.99. The limits of detection (LODs, S/N = 3) of the method were between 0.001J μg/kg and 1 (μg/kg, and the limits of quantification (LOQs, S/N = 10) were between1] 0. 003 μg/kg and 5 μg/kg. The recoveries of BAs spiked in eight matrices were between 73.9% and 106.3% at the spiked levels ranged from 0. 003 μg/kg to 50 (μg/kg. The relative standard deviations (RSDs, n =6) were in the ranges of 5. 65% - 18. 6%. The method can meet the requirements of the daily work for the determination of BAs in animal-derived foodstuffs.


Long Y.,CAS Wuhan Institute of Hydrobiology | Li L.,Xiamen Ocean Vocational College | Li Q.,CAS Wuhan Institute of Hydrobiology | He X.,CAS Wuhan Institute of Hydrobiology | Cui Z.,CAS Wuhan Institute of Hydrobiology
PLoS ONE | Year: 2012

Temperature influences nearly all biochemical, physiological and life history activities of fish, but the molecular mechanisms underlying the temperature acclimation remains largely unknown. Previous studies have identified many temperature-regulated genes in adult tissues; however, the transcriptional responses of fish larvae to temperature stress are not well understood. In this study, we characterized the transcriptional responses in larval zebrafish exposed to cold or heat stress using microarray analysis. In comparison with genes expressed in the control at 28°C, a total of 2680 genes were found to be affected in 96 hpf larvae exposed to cold (16°C) or heat (34°C) for 2 and 48 h and most of these genes were expressed in a temperature-specific and temporally regulated manner. Bioinformatic analysis identified multiple temperature-regulated biological processes and pathways. Biological processes overrepresented among the earliest genes induced by temperature stress include regulation of transcription, nucleosome assembly, chromatin organization and protein folding. However, processes such as RNA processing, cellular metal ion homeostasis and protein transport and were enriched in genes up-regulated under cold exposure for 48 h. Pathways such as mTOR signalling, p53 signalling and circadian rhythm were enriched among cold-induced genes, while adipocytokine signalling, protein export and arginine and praline metabolism were enriched among heat-induced genes. Although most of these biological processes and pathways were specifically regulated by cold or heat, common responses to both cold and heat stresses were also found. Thus, these findings provide new interesting clues for elucidation of mechanisms underlying the temperature acclimation in fish. © 2012 Long et al.


PubMed | Xiamen Ocean Vocational College, State Oceanic Administration, University of Guelph and Fisheries Research Institute of Fujian
Type: | Journal: Food microbiology | Year: 2016

As filter-feeding bivalves, oysters can accumulate microorganisms into their gills, causing spoilage and potential safety issues. This study aims to investigate the changes in the gill microbiota of oysters packed under air and modified atmospheres (MAs, 50% CO

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