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Chen S.,Xiamen Entry Exit Inspection and Quarantine Technology Center | Zhang J.,Agilent Technologies | Chen W.,Xiamen Entry Exit Inspection and Quarantine Technology Center | Zhang Y.,Xiamen Entry Exit Inspection and Quarantine Technology Center | And 3 more authors.
Food Control | Year: 2012

A convenient and quick method based on real-time polymerase chain reaction (PCR) was established to identify 10 grouper species in China. According to the sequence of mitochondrial cytochrome oxidase subunit I (COI) gene, primers and probe were designed. PCR amplifications of 129 bp of COI gene sequences from 10 grouper species of four genera (Epinephelus, Promicrops, Plectropomus and Cromileptes) were compared with that of 30 other fish species. PCR amplifications were only observed in 10 grouper species. The result suggested that real-time PCR can be a powerful tool for grouper species diagnoses. © 2012. Source


Chen S.,Xiamen Entry Exit Inspection and Quarantine Technology Center | Zhang Y.,Xinglin Entry Exit Inspection and Quarantine Bureau | Li H.,China National Accreditation Service for Conformity Assessment | Wang J.,Xiamen Entry Exit Inspection and Quarantine Technology Center | And 3 more authors.
Food Control | Year: 2014

Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis and lab-on-a-chip system were used to identify 62 commercial fish species in Taiwan Strait. The fish species include 10 groupers, 12 bream species, 9 Sciaenidae species, 5 puffer species and 26 other fish species. A fragment of 464bp length of mitochondrial cytoehrome b gene was amplified by PCR and the products were digested with restriction enzymes DdeI, HaeIII and NlaIII, individually. The fragments generated after digestion were further resolved on the DNA chip. The results demonstrated that PCR-RFLP analysis and lab-on-a-chip system provided a fast, easy, automated and reliable analysis approach and it will be useful for the control of the adulteration of food with fish tissue content in Taiwan Strait. © 2014 Elsevier Ltd. Source

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