Patel M.,Xcelris Genomics Research Center |
Manvar T.,Xcelris Genomics Research Center |
Apurwa S.,Xcelris Genomics Research Center |
Ghosh A.,Xcelris Genomics Research Center |
And 2 more authors.
Molecular Biology Reports | Year: 2014
Grapefruit (Citrus pardisi) is a popular citrus fruit that is a cross between a sweet orange and pummelo. This research article focuses on an in silico approach for comparative analysis of C. paradisi green flavedo (GF) and ethylene treated flavedo (ETF) transcriptome data. Our pathway analysis provides comprehensive information of genes playing significant role in different stages of ripening in fruit. De novo assembly was carried out using six different assemblers namely GS assembler, SeqMan NGEN, Velvet/Oases, CLC, iAssembler and Cortex followed by subsequent meta-assembly, annotation and pathway analysis. We conclude that de novo transcriptome assembly using meta-assembly approach is used to increase assembly quality in comparison to single assembler. © Springer Science+Business Media 2014.