Wyatt Technology Corporation

Hollister, CA, United States

Wyatt Technology Corporation

Hollister, CA, United States
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Some D.,Wyatt Technology Corporation
Biophysical Reviews | Year: 2013

While light scattering has long been applied to the analysis of biomolecular interactions, recent advances have extended the practical use of light scattering techniques to cover a rather broad range of phenomena. In this paper I review essential light scattering theory as applied to specific interactions under thermodynamically ideal conditions and present examples showing how light scattering elucidates the dynamic equilibrium and kinetic behavior of proteins and other biomacromolecules. © 2013 The Author(s).


Patent
Wyatt Technology Corporation | Date: 2013-11-27

A pressure transducer is disclosed wherein no wetted areas have been welded. A cavity is milled into the back of each of the blocks of a material which will make up the body of the transducer. Pickup coils are placed into these cavities and are held in place generally with epoxy cement. With the coils mounted within the sensor body, the surface which will be exposed to the sample or reference fluids is comprised of a single, solid material with no welding joints. Further, as the sensor block half is made of a single, solid material, fluid fitting connections may be machined directly into the body. The pickup coil placed within the improved sensor body may be wound on an open frame of nickel superalloy (NiSA). Another embodiment involves coating or encapsulating the sensing membrane within a soft, non-magnetic material protecting it from corrosion.


Patent
Wyatt Technology Corporation | Date: 2016-04-15

An electrode for use in instruments capable of measuring the electrophoretic mobility of particles in solution is disclosed. The electrode is comprised of an inexpensive support member, generally made of titanium, onto a flat surface of which has been connected, generally by microwelding, a flat electrically conductive but chemically inert foil member, preferably platinum. A uniform texture can be generated on the exposed surfaces of the electrode by various means including tumbling the electrode with an abrasive. An oxide layer can be generated on the support member by soaking the composite electrode in an appropriate medium, protecting the exposed surface of the support member from fluid contact with the sample solution, while the foil member, unaffected by the oxidation process, is able to contact the sample solution.


Patent
Wyatt Technology Corporation | Date: 2013-11-27

A cuvette for use with light scattering detectors is disclosed. A trough or moat within the cuvette can be filled with solvent which is not in fluid contact with the sample to be measured. This solvent moat creates saturated vapor pressure in the chamber preventing evaporation from the sample when the cuvette is capped. The cuvette itself may be made of an inexpensive polymer which can be polished to high optical quality while still being moldable in complex forms capable of enabling further utility, such as extra griping surfaces, identification tabs allowing the detection instrument to determine the cuvette model, and various sample chamber forms. The novel cuvette may have extremely small sample volumes, while allowing significant overfill of the measurement chamber, improving ease of sample loading. The polymers used may be relatively inexpensive, and therefore the cuvette can generally be discarded after a single use.


Patent
Wyatt Technology Corporation | Date: 2016-01-20

A method is presented by which means small particles in solution, of various structures and of sizes up to several hundred nanometers, may be measured by light scattering means. An inventive technique is described, permitting the traditional Rayleigh-Gans approximation to be extended, allowing thereby measurement of the mean square radii of particles over a greater size range. Such determinations obviate the need to fit the collected data to a particular closed form model of which, in any event, only a few exist. The new method is particularly important for determining structural features of irregular particles whose scattering depends on their orientation with respect to the direction of the incident illumination.


Patent
Wyatt Technology Corporation | Date: 2014-07-02

A method and apparatus are disclosed which enable the reduction of sample carryover in the measurement cell of an analytical instrument. A sample cell is defined as a region sealed within a first o-ring. Located outside of said sample region is another o-ring which seals and defines a seal wash region as the region between the first and second o-ring. After the fluid sample is injected into the measurement cell a pressure is applied to the seal wash region, forcing the first o-ring to the innermost extent of the groove in which it sits, expelling any trapped solvent and removing from the measurement cell a significant dead volume while the cell is flushed and prepared for a new sample and corresponding measurement.


An electrode for use in instruments capable of measuring the electrophoretic mobility of particles in solution is disclosed. The electrode is comprised of an inexpensive support member, generally made of titanium, onto a flat surface of which has been connected, generally by microwelding, a flat electrically conductive but chemically inert foil member, preferably platinum. A uniform texture can be generated on the exposed surfaces of the electrode by various means including tumbling the electrode with an abrasive. An oxide layer can be generated on the support member by soaking the composite electrode in an appropriate medium, protecting the exposed surface of the support member from fluid contact with the sample solution, while the foil member, unaffected by the oxidation process, is able to contact the sample solution.


Patent
Wyatt Technology Corporation | Date: 2014-11-25

A lid for a multiwell plates which allows improved light scattering measurement of liquid samples within the wells of a multiwell plate, and which at the same time mitigates evaporation from said samples is disclosed. A surface element protrudes from the bottom of the lid into the fluid in a well. The protruding element may be hollow or solid, and the beam of light, directed into the element may act to capture or direct the beam while preventing backscatter from reaching the light scattering detector or detectors. The protruding element may thus direct the beam from the well without the beam having to pass through a fluid/air interface. The angle and shape of the lid surfaces may be optimized to minimize or eliminate back-reflection. Light absorbing and/or light blocking colorization may also be employed to minimize or eliminate back reflection. Evaporation is controlled by physically capping the well with the lid, either sealing against the face at the top of the well or the inside surface of the well.


Patent
Wyatt Technology Corporation | Date: 2014-12-23

A method is presented by which means small particles in solution, of various structures and of sizes up to several hundred nanometers, may be measured by light scattering means. An inventive technique is described, permitting the traditional Rayleigh-Gans approximation to be extended, allowing thereby measurement of the mean square radii of particles over a greater size range. Such determinations obviate the need to fit the collected data to a particular closed form model of which, in any event, only a few exist. The new method is particularly important for determining structural features of irregular particles whose scattering depends on their orientation with respect to the direction of the incident illumination.


Patent
Wyatt Technology Corporation | Date: 2015-08-27

This invention enables high throughput detection of small molecule effectors of particle association, as well as quantification of association constants, stoichiometry, and conformation. Particle refers to any discrete particle, such as a protein, nucleic acid, carbohydrate, liposome, virus, synthesized polymer, nanoparticle, colloid, latex sphere, etc. Given a set of particle solutions having different concentrations, dynamic light scattering measurements are used to determine the average hydrodynamic radius, r_(avg), as a function of concentration. The series of r_(avg )as a function of concentration are fitted with stoichiometric association models containing the parameters of molar mass, modeled concentrations, and modeled hydrodynamic radii of the associated complexes. In addition to the r_(avg )value analysis, the experimental data may be fit/analyzed in alternate ways. This method may be applied to a single species that is self-associating or to multiple species that are hetero-associating. This method may also be used to characterize and quantify the association between a modulator and the associating species.

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