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Zhang K.,Jiangsu Institute of Nuclear Medicine | Xie M.,Jiangsu Institute of Nuclear Medicine | Zhou B.,Jiangsu Institute of Nuclear Medicine | Hua Y.,Wuxi No. 3 Peoples Hospital | And 5 more authors.
Biosensors and Bioelectronics | Year: 2013

Quantitative protein bioanalysis in complex biological fluids presents considerable challenges in biological studies and medical diagnosis. The major obstacles are the background signals from the biological fluids and sensors themselves. Because the europium ion (Eu (III)) has the much longer fluorescence lifetime (1ms) than that of the background (5ns), time-resolved method can be widely used to eliminate the biological background. So, we report here an aptamer-based sensor (aptasensor) for time-resolved fluorescence assay of adenosine deaminase (ADA). This aptasensor employs two oligonucleotides labeled with DIG and biotin, respectively. The DNA1 (an oligonucleotide modified with biotin) is immobilized at a streptavidin-modified plate surface via the biotin-avidin bridge, and the DIG which is modified on the DNA2 serves as an affinity tag for the Eu3+ labeled anti-DIG (Eu-anti-DIG) binding. If the adenosine is binding with DNA1, it will make the DNA1 in the closed state with a close-packed tight structure, which forbids the DNA2 approaching. And if the ADA is added into the mixture, the DNA1 unbends, because of the adenosine is transformed to inosine catalyzed by the ADA. Then DNA2 could hybridize with DNA1. Accordingly, the DIG finds Eu-anti-DIG and the Eu-anti-DIG will give a remarkable fluorescent signal. The detection limit of the aptasensor can be lowered to 2UL-1, which can meet the clinical requirement of ADA cutoff value (4UL-1). Moreover, we were able to detect ADA in human serum quantitatively. Combined with time-resolved based measurements and aptasensor, this strategy holds great potential in protein analysis. © 2012 Elsevier B.V.

Hu M.-Z.,Jiangnan University | Zhou B.,Jiangnan University | Mao H.-Y.,Wuxi No. 3 Peoples Hospital | Sheng Q.,Jiangnan University | And 4 more authors.
International Heart Journal | Year: 2016

Sirt1 is a highly conserved nicotinamide adenine dinucleotide (NAD+) dependent histone deacetylase which plays an important role in heart diseases. Studies performed with Sirt1 activators indicated that it protects cells from ischemia/ reperfusion (I/R) injury. The protective effects of H2S against I/R injury also have been recognized. Hence, the present study was designed to explore whether Sirt1/PGC-1α participates in the protection of exogenous H2S postconditioning against I/R injury in isolated rat hearts. Isolated rat hearts were subjected to 30 minutes of global ischemia followed by 60 minutes of reperfusion after 20 minutes of equilibrium. During this procedure, the hearts were exposed to NaHS (10 μmol/L) treatment in the absence or presence of the selective Sirt1 inhibitor EX-527 (10 μmol/L). NaHS exerted a protective effect on isolated rat hearts subjected to I/R, as shown by the improved expression of Sirt1/PGC-1α associated with restoration of Sirt1 nuclear localization, cardiac function, decreased myocardial infarct size, decreased myocardial enzyme release, and several biochemical parameters, including up-regulation of the ATP and SOD levels, and down-reg-ulation of the MDA level. However, treatment with EX-527 could partially prevent the above effects of NaHS postconditioning. These results indicate that H2S confers protective effects against I/R injury through the activation of Sirt1/PGC-1α. © 2016 International Heart Journal Association. All rights reserved.

Yang M.,Wuxi No. 3 Peoples Hospital
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery | Year: 2010

To assess the efficacy of the extra long scapular-lateral thoracic-ilioinguinal siamese flap to repair the contracture deformity of perineal scar caused by burn and to discuss its characteristics. From January 2008 to August 2009, 9 patients with contracture deformity of perineal scar after deep II degree to III degree burn were treated. There were 7 males and 2 females aged from 22 to 54 years (35.4 years on average). The course of disease ranged from 8 months to 5 years. All cases had central type of perineal scar. Among the cases, 3 cases were complicated by abdominal scar, 4 cases by legs scar, and 2 cases by abdominal and legs scar. Scar ulcer was observed in 2 cases. The opening-closing angle of bilateral lower extremities was (29.4 +/- 8.8) degrees. And anus could not expose entirely so that squatting and relieving the bowels were difficult in 6 cases. Defect areas after scar resection ranged from 20 cm x 6 cm to 28 cm x 8 cm. The size of extra long scapular-lateral thoracic-ilioinguinal siamese flap ranged from 35 cm x 12 cm to 58 cm x 15 cm. The donor sites were sutured directly. Blister and necrosis occurred in 1 case and was cured after dressing changed, and others flaps survived with wounds primary healing. Incision at donor site healed by first intention. All cases were followed up 6-12 months. The perineal function improved and the partial deformities were corrected. The opening-closing angle of bilateral lower extremities increased to (75.6 +/- 11.3) degrees, showing significant difference between pre- and post-operation (P < 0.05). The functions of squatting and relieving the bowels recovered well. The perineal scar adhesion recurred in 2 cases after 6 months of operation and were cured after scar resection and expanding flaps transposition. In view of large donating region, great facility for transposition, stable and sufficient blood supply, reutilization as expanded flap, it was an effective treatment and a beneficial trial by applying the transposition of the extra long scapular-lateral thoracic-ilioinguinal siamese flap for contracture deformity of perineal scar caused by burn.

Gao F.,Shandong University | Yang M.L.,Wuxi No. 3 Peoples Hospital | Zhao Z.M.,Peking Union Medical College | Sun X.M.,Peking Union Medical College | And 8 more authors.
Chinese Medical Journal | Year: 2014

Background Maxillary anterior segmental distraction osteogenesis (MASDO) is a recently used method for correction of severe maxillary retrusion in cleft lip and palate (CLP) patients. In this article, we evaluated the feasibility of MASDO using rigid external distraction (RED) and rapid orthodontic tooth movement to correct severe maxillary retrusion in CLP patients. Methods Fourteen male and five female complete CLP patients between the ages of 18 and 22 years (mean age 19.7 years) at the time of distraction, with severe maxillary retrusion, were treated with the rigid external distraction (RED) device after maxillary anterior osteotomy. Rapid orthodontic tooth movement was started one week after the MASDO. Standard profile photographic, cephalometric films were obtained preoperatively and after therapy. Sella-nasion-point A (SNA) and Sella-nasion-point B (SNB) angles were measured to reflect changes in maxillary and mandibular position, and the distance between anterior nasal spine and posterior nasal spine (ANS-PNS) was measured to represent the maxillary dental arch length. Results The SNA angle increased from an average of 74.6° (range 73.0°-78.0°), preoperatively, to 83.4° (range 78.6°-88.0°) after the RED was removed (P <0.01). All cases of severe maxillary retrusion were improved. Nine patients' profiles became harmonious after therapy. One patient had a bimaxillary protrusion deformity and needed further surgery. The regenerate alveolar crest and edentulous space on both segments was almost completely eliminated after rapid orthodontic tooth movement. Conclusion MASDO with the RED system and rapid orthodontic tooth movement is a successful way of correcting severe maxillary retrusion in CLP patients.

Jiang C.-B.,Nanjing University | Wei M.-G.,Soochow University of China | Tu Y.,Nanjing University | Zhu H.,Soochow University of China | And 3 more authors.
Evidence-based Complementary and Alternative Medicine | Year: 2015

Objectives. We investigated the action of triptolide in rats with adriamycin-induced nephropathy and evaluated the possible mechanisms underlying its protective effect against podocyte injury. Methods. In total, 30 healthy male Sprague-Dawley rats were randomized into three groups (normal group, model group, and triptolide group). On days 7, 28, 42, and 56, 24 h urine samples were collected. All rats were sacrificed on day 56, and their blood and renal tissues were collected for determination of biochemical and molecular biological parameters. Expression of miRNAs in the renal cortex was analyzed by a biochip assay and RT-PCR was used to confirm observed differences in miRNA levels. Results. Triptolide decreased proteinuria, improved renal function without apparent adverse effects on the liver, and alleviated renal pathological lesions. Triptolide also elevated the nephrin protein level. Furthermore, levels of miR-344b-3p and miR-30b-3p were elevated in rats with adriamycin-induced nephropathy, while triptolide treatment reversed the increase in the expression of these two miRNAs. Conclusions. These results suggest that triptolide may attenuate podocyte injury in rats with adriamycin-induced nephropathy by regulating expression of miRNA-344b-3p and miRNA-30b-3p. © 2015 Chun-Bo Jiang et al.

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