Time filter

Source Type

Qi Z.,Soochow University of China | Xue J.,Soochow University of China | Zhang Y.,Wuxi Hospital for Maternal and Child Health Care | Wang H.,Changshu Leiyunshang Pharmaceutical Co. | Xie M.,Soochow University of China
Planta Medica | Year: 2011

The objectives of this study were to determine the effect of osthole on the insulin resistance (IR) in high-fat and high-sucrose-induced fatty liver rats and to investigate its potential mechanisms. The rat model was established by orally feeding high-fat and high-sucrose emulsion by gavage for 9 weeks. The experimental rats were treated with osthole 5 and 10 mg/kg, lipanthyl 30 mg/kg, and rosiglitazone 4 mg/kg after oral high-fat and high-sucrose emulsion for 6 weeks and were sacrificed 4 weeks after administration. The total cholesterol (TC), triglycerides (TG), and free fatty acids (FFA) in serum and hepatic tissue, fasting blood glucose (FBG), fasting serum insulin (FINS), serum adiponectin, and liver weight were measured. The homeostasis model assessment of insulin resistance (HOMAIR) and coefficient of hepatic weight were calculated. The results showed that after treatment with osthole, the serum levels of TC, TG, and FFA, the contents of TG and FFA in hepatic tissue, and body weight gain were lowered, especially in the osthole 10 mg/kg group (p < 0.05 or p < 0.01). Moreover, the histological evaluation of liver specimens demonstrated that the steatosis and inflammation in liver in osthole-treated groups were improved, especially in the 10 mg/kg group (p < 0.05). Importantly, the levels of FBG, FINS, and HOMAIR in the osthole 10 mg/kg group were decreased (p < 0.01), while the level of serum adiponectin in the osthole-treated groups, like PPARα agonist lipanthyl and PPARγ agonist rosiglitazone, was increased (p < 0.05). These results revealed that osthole could improve the IR induced by high-fat and high-sucrose emulsion in fatty liver rats, and its mechanism might be associated with increment of adiponectin release via activation of PPARα/γ pathway. © 2010 Georg Thieme Verlag KG Stuttgart. New York. Source

Tang Q.,Wuxi Hospital for Maternal and Child Health Care | Tang Q.,Nanjing Medical University | Wu W.,Wuxi Hospital for Maternal and Child Health Care | Wu W.,Nanjing Medical University | And 10 more authors.
PLoS ONE | Year: 2013

Background: Fetal growth restriction (FGR) is an important but poorly understood condition of pregnancy, which results in significant fetal, neonatal and long-term morbidity and mortality. Novel research has suggested that altered miRNA expression in the plasma and placenta is associated with adverse pregnancy. We hypothesized that aberrant expression of microRNA-141 (miR-141) in the placenta is associated with FGR. Additionally, expression levels of predicted target genes of miR-141 were also analyzed in placental tissues of FGR and normal controls. Methodology/Principal Findings: Using quantitative real time PCR, we analyzed the expression level of miR-141 and its target genes in placentas of FGR pregnancies (n = 21) and normal controls (n = 34). Western blot was used to detect the protein expression level of the target genes of miR-141. MiR-141 showed significant up regulation in FGR and significant down regulation of its targets, i.e. E2F transcription factor 3 (E2F3) protein, pleiomorphic adenoma gene 1 (PLAG1) mRNA and protein. Moreover, a positive correlation was found between PLAG1 and insulin-like growth factor 2 (IGF2) expression levels (Spearman r = 0.56, p<0.0001). MiR-141 yields an AUC of 0.83 with 88.5% sensitivity and 71.7% specificity for separating FGR from normal controls. This study indicates that miR-141 may be diagnostically important in FGR. Conclusions/Significance: Our results indicate that aberrant high expression level of miR-141 might play important roles in the pathogenesis of FGR by suppressing E2F3 and PLAG1. We propose that miR-141 may participate in a miR-141-PLAG1-IGF2 network relating to FGR development. These findings may provide new targets via miR-141 in diagnosis and therapy of FGR in the future. © 2013 Tang et al. Source

Guo C.,Wuxi Hospital for Maternal and Child Health Care | Xiao J.,Wuxi Hospital for Maternal and Child Health Care | Wang J.,Wuxi Hospital for Maternal and Child Health Care | Yang L.,Wuxi Hospital for Maternal and Child Health Care | Tang Y.,Wuxi Hospital for Maternal and Child Health Care
Chinese Journal of Medical Genetics | Year: 2015

Objective To verify the diagnosis of Angelman syndrome(AS) in a proband in order to provide prenatal diagnosis for his family. Methods Array comparative genome hybridization (array-CGH) and fluorescence in situ hybridization (FISH) on metaphase chromosomes were performed. Results The karyotype of the proband was normal, and a regional deletion of 15qll. 1-11.2 was detected by array-CGH. FISH analysis has confirmed loss of heterozygosity in 15qll. 2. No positive results were obtained by array-CGH or karyotype analysis. Amniotic fluid sample was taken from the proband's mother upon her subsequent pregnancy. The karyotype of the fetus was normal, but SNP microarray chip analysis has identified loss of heterozygosity in 8p23. l-p22. As no abnormality was observed by ultrasound and other prenatal examinations, the pregnancy was recommended to continue to full-term, and a healthy infant was born. Conclusion Clinically suspected AS can be diagnosed by array-CGH and FISH. The result may facilitate accurate genetic counseling and prenatal diagnosis for the affected family. Source

Zhang Y.,Wuxi Hospital for Maternal and Child Health Care | Song H.,Wuxi Hospital for Maternal and Child Health Care | Wen H.,Wuxi Hospital for Maternal and Child Health Care | Zhang X.,Wuxi Hospital for Maternal and Child Health Care | And 2 more authors.
Cancer Research and Clinic | Year: 2015

Objective To investigate the effect of osthole on the proliferation and apoptosis of breast cancer cell line MCF-7 and its potential mechanisms. Methods Breast cancer cell line MCF-7 was treated by osthole 0, 25, 50, 100, 150 and 200 μmol/L respectively. MTT method was used to detect cell survival rate. HE staining was used to observe morphological changes, Annexin V-PI flow cytometry was used to analyze cell apoptosis, and RT-PCR and Western blot method were used to detect the mRNA and protein expression of peroxisome proliferator-activated receptor γ (PPARγ) and farnesoid X receptor (FXR), respectively. Results MTT assay showed that strong cytotoxicity of cell line MCF-7 was induced after administration of osthole for 72 h in a dose-dependent manner. Especially, the maximum inhibitory rate, 73.0 % appeared in the 200 μmol/L group. HE staining showed that the number of MCF-7 cells decreased, hyperchromatic nuclei and apoptotic bodies appeared after treatment with osthole for 72 h in a significant dose-effect manner. Flow cytometric analysis revealed that osthole could induce extensive apoptosis in MCF-7 cultures after treatment for 72 h compared with normal group (P < 0.05, P < 0.01). In particular, when the concentration of osthole reached 50 μmol/L, the proportion of early apoptotic cells was significantly increased in a dose-dependent manner (P < 0.01), especially. The maximum apoptosis rate (46.2±9.0) % appeared in the 200 μmol/L group, which was consistent with the results obtained from MTT assays. Moreover, osthole could significantly increased PPARγ and FXR mRNA and protein expressions (P < 0.01). Conclusion These data suggest that osthole could inhibit the proliferation of breast cancer MCF-7 cells and promote its apoptosis, which might be associated with the regulation of PPARγ and FXR-mediated target genes involved in cell growth and metabolism. Source

Discover hidden collaborations