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Liu Y.,Wuxi Higher Health Vocational Technology School | Xie K.-M.,Suzhou University | Yang G.-Q.,Suzhou University | Bai X.-M.,Suzhou University | And 5 more authors.
Cancer Chemotherapy and Pharmacology | Year: 2010

We have previously shown that the expression of glucosylceramide synthase (GCS) gene in drug-resistant K562/AO2 human leukemia cell was higher than that in drug-sensitive K562 cell, and the sensitivity to adriamycin of K562/AO2 cell was enhanced by inhibiting GCS. It is concluded that the overexpression of GCS gene is one of the reasons which lead to multidrug resistance (MDR) of leukemia cell. Meanwhile, we also found that higher expression of Bcl-2 gene and protein were exhibited in K562/AO2 cell compared with K562 cell. Basing on this, we hypothesized that the high expression of GCS gene which results in MDR of leukemia cell is correlated with Bcl-2 signal transduction. In order to validate the hypothesis, the inhibition of GCS gene in K562/AO2 cell was observed by using chemical suppressor PPMP and siRNA targeted at GCS, and applying RT-PCR and flow cytometry, the expression levels of apoptosis-related gene Bcl-2 and Bax were analyzed before and after inhibiting GCS gene in K562/AO2 cell. The results demonstrated that the gene and protein of Bcl-2 in K562/AO2 cell were both down-regulated significantly after GCS gene being inhibited; however, the Bax mRNA expression had no apparent change in different groups. This suggested that GCS gene may contributed to MDR of human leukemia cell K562/AO2 by Bcl-2 signal transduction. © Springer-Verlag 2009. Source

Fan Y.-F.,Nanjing Medical University | Wu Y.-M.,Nanjing Medical University | Liu H.,Nanjing Medical University | Yu Y.,Fudan University | And 4 more authors.
Human Immunology | Year: 2014

Helicobacter pylori infection is a risk factor for gastric cancer. In addition, toll-like receptor 4 (TLR4) plays a fundamental role in pathogen recognition and activation of innate immunity. This study investigated the association of TLR4 polymorphisms with a risk of intestinal metaplasia (IM) and intraepithelial neoplasia (IN) in a Chinese Han population. This study analyzed TLR4 gene polymorphisms in 333 patients (IM, 193 cases; IN, 140 cases) and 312 atypia-free controls in a Chinese Han population using a Taqman allelic discrimination assay. The TLR4 single nucleotide polymorphisms +896A/G and +1196C/T were not associated with the risk of IM or IN. However, the single-locus analysis showed that the C allele of TLR4+2856T/C had significantly reduced risk of IM and IN [adjusted odds ratio (OR). = 0.42; 95%CI. = 0.29-0.62 and OR. = 0.62; 95%CI. = 0.41-0.93, respectively] compared with the wild-type homozygote (TT). The frequencies of TLR4+2856T/C TC and T carrier were significantly lower in patients with Sydney's slight IM and low grade IN (P<. 0.01 and P= 0.01, respectively), while the TC genotype showed a lower risk of moderate IM compared to healthy controls (P= 0.045). In addition, the data revealed that H. pylori infection, heavy alcohol consumption and high salt uptake were associated with a higher susceptibility for developing this neoplasm. TLR4 rs10759932 TC and C carriers were associated with a lower risk in developing precancerous lesions in the stomach in a Chinese Han population. © 2013 American Society for Histocompatibility and Immunogenetics. Source

Wang Q.,Jiangnan University | Ji J.,Jiangnan University | Jiang D.,Jiangnan University | Wang Y.,Wuxi Higher Health Vocational Technology School | And 2 more authors.
Analytical Methods | Year: 2014

A novel electrochemical sensor for acrylamide (AM) detection based on molecularly imprinted polymer (MIP) membranes was constructed. p-Aminothiophenol (P-ATP) and AM were assembled on the surface of a gold nanoparticle (AuNP) modified glass carbon electrode (GCE) by the formation of Au-S bonds and hydrogen-bonding interactions, and polymer membranes were formed by electropolymerization in a polymer solution containing P-ATP, HAuCl4, tetrabutylammonium perchlorate (TBAP) and a dummy template molecule propanamide (PMA). A novel molecularly imprinted sensor (MIS) was obtained after the removal of PMA. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) measurements were used to monitor the electropolymerization process and its optimization, which was further characterized by scanning electron microscopy (SEM). The linear response range of the MIS was between 1 × 10 -12 and 1 × 10-7 mol L-1, with a detection limit of 0.5 × 10-12 mol L-1. This research provides a fast, sensitive and real-time method for the detection of AM in a real sample without complex pretreatment and with average recoveries higher than 95% and a relative standard deviation (RSD) lower than 3.73%. All the obtained results indicate that the MIS is an effective electrochemical technique to determine AM in real-time and in a complicated matrix. This journal is © the Partner Organisations 2014. Source

Yao W.,Wuxi Higher Health Vocational Technology School | Zou H.-J.,Wuxi Higher Health Vocational Technology School | Sun D.,Wuxi Higher Health Vocational Technology School | Ren S.-Q.,Nanjing Medical University
Brain Research | Year: 2013

Beta-amyloid peptide (Aβ) has a causal role in the pathophysiology of Alzheimer's disease (AD). Recent studies indicate that Aβ can disrupt excitatory glutamatergic synaptic function at synaptic level. However, the underlying mechanisms remain obscure. In this study, we recorded evoked and spontaneous EPSCs in hippocampal CA1 pyramidal neurons via whole-cell voltage-clamping methods and found that 1 μM Aβ can induce acute depression of basal glutamatergic synaptic transmission through both presynaptic and postsynaptic dysfunction. Moreover, we also found that Aβ-induced both presynaptic and postsynaptic dysfunction can be reversed by the inhibitor of protein phosphatase 2B (PP2B), FK506, whereas only postsynaptic disruption can be ameliorated by the inhibitor of PP1/PP2A, Okadaic acid (OA). These results indicate that PP1/PP2A and PP2B have overlapping but not identical functions in Aβ-induced acute depression of excitatory glutamatergic synaptic transmission of hippocampal CA1 pyramidal neurons. © 2013 Elsevier B.V. Source

Wu Y.,Nanjing Medical University | Fan Y.,Nanjing Medical University | Jiang Y.,Wuxi Higher Health Vocational Technology School | Wang Y.,Nanjing Medical University | And 2 more authors.
Journal of Cancer Research and Therapeutics | Year: 2013

Purpose: To investigate the association between various risk factors and precancerous lesion of gastric cancer (PLGC) in patients from eastern China. Materials and Methods: 501 cases of PLGC and 523 cases of superficial gastritis were included. A comparative study of the relation between different risk factors and PGLC was performed. Results: Statistical differences were noted in a series of indexes including Helicobacter pylori (HP) infection, family history of esophageal cancer (EC), gastric cancer (GC) and chronic atrophic gastritis (CAG), a history of CAG, gastric polyps (GP) and gastric ulcer (GU), usage of non-steroids (e.g., aspirin), gastroesophageal reflux disease (GERD), consuming alcohol, eating food rich in nitroso compounds, irregular eating habits with no breakfast, ingestion of smoked meat, fried food and spicy food, anxiety and depression. The risk factors associated with PLGC ranked in an order of a history of CAG, GP, family history of GC, usage of non-steroids (e.g., aspirin), ingestion of spicy food frequently, HP infection, family history of EC, consuming alcohol, anxiety, a history of GU, GERD and family history of CAG. Conclusions: A history of CAG was most associated with PLGC in patients from eastern China, followed by a history of GP and family history of GC. Source

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