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Yang W.-X.,Nanjing Medical University | Jin R.,Nanjing Medical University | Jiang C.-M.,Zhejiang University of Science and Technology | Wang X.-H.,Nanjing Medical University | And 6 more authors.
FEBS Letters

Abstract Orosomucoid 1-Like Protein 3 (ORMDL3) is an asthma candidate gene and Casitas B lineage lymphoma b (Cbl-b), an E3 ubiquitin ligase, is a critical factor in maintaining airway immune tolerance. However, the association of Cbl-b with ORMDL3 for asthma is unclear. Here, we show that expression of ORMDL3 is significantly increased and shows a strong linear correlation with decreased Cbl-b in the peripheral blood of recurrent wheeze patients. To elucidate the molecular mechanisms underlying this correlation, we identified that Cbl-b suppressed the transcriptional activity and mRNA expression of ORMDL3 in vivo. Further investigation showed that phosphorylation of signal transducer and activator of transcription 6 (STAT6) was induced by interleukin 4 bound to the ORMDL3 promoter, while Cbl-b reduced the phosphorylation of STAT6. Our results show that Cbl-b suppresses human ORMDL3 expression through STAT6. © 2015 Federation of European Biochemical Societies. Source

Wang W.,Jiangsu Institute of Parasitic Diseases | Wang W.,Key Laboratory on Technology for Parasitic Disease Prevention and Control | Li T.-Y.,Wuxi Childrens Hospital | Ji Y.,Nanjing Medical University | And 10 more authors.
Parasitology Research

Praziquantel is currently the only drug of choice for the treatment of human Schistosoma japonicum infections, and praziquantel-based chemotherapy has been proved to be generally effective to control the morbidity and reduce the prevalence and intensity of S. japonicum infections. However, the potential emergence of praziquantel resistance in S. japonicum seriously threatens the elimination of this neglected tropical disease in China. The purpose of this study was designed, in mouse animals, to evaluate the in vivo efficacy of artemether and artesunate against praziquantel non-susceptible S. japonicum. Mice infected with a praziquantel non-susceptible isolate and a praziquantel-susceptible isolate of S. japonicum were treated with artemether and artesunate at a single oral dose of 300 mg/kg given once on each of days 7-8 and 35-36 post-infection to assess the efficacy against juvenile and adult worms. Administration with artemether and artesunate at a single oral dose of 300 mg/kg on each of days 7-8 post-infection resulted in total worm burden reductions of 72.8 and 73.5 % in mice infected with praziquantel-susceptible S. japonicum, and 77.9 and 74.1 % in mice infected with the non-susceptible isolate (both P values >0.05), while the same treatments given on days 35-36 post-infection reduced total worm burdens by 71.4 and 69.6 % in mice infected with the susceptible isolate, and 75.3 and 69.6 % in mice infected with the non-susceptible parasite (both P values >0.05). It is concluded that there is no evidence for reduced susceptibility of artemether and artesunate in praziquantel non-susceptible S. japonicum. © 2013 Springer-Verlag Berlin Heidelberg. Source

Liu Y.,Wuxi Childrens Hospital | Xu X.,Neurology | Hua Y.,Wuxi Childrens Hospital | Xu J.,Wuxi Childrens Hospital | Hui X.,Wuxi Childrens Hospital
International Journal of Clinical and Experimental Medicine

More and more evidences suggestted that ApoE plays an important role in modulating the systemic and central nervous inflammatory responses. However, there is a lack of exacted mechanism of ApoE. In this study, we aimed to investigate whether apolipoprotein E (ApoE) induced inflammatory responses and apoptosis in neonatal mice brain from ApoE deficient (ApoE-/-) and wildtype (WT). Compared to control group, the microglia cell from ApoE-/- mice showed more severe inflammation and cell death such as iNOS and IL-1β. Furthermore, anti-inflammatory such as TGF-β, IL-10 from microglia and astrocytes in ApoE-/- mice were decreased. On the other way, TGF-β from astrocytes can inhibit inflammation factors secretion from microglia. Our findings suggested that the anti- inflammation factor such as IL-10 mainly from microglia and TGF-β mainly from astrocyte is significant decreased after Loss of ApoE function in ApoE-/- mice which induced severe inflammation. Furthrtmore, anti- inflammation factor such as IL-10 and TGF-β Therefore, we conclude that apolipoprotein E knockout induced inflammatory responses related to microglia in neonatal mice brain via astrocytes. © 2015, Int J Clin Exp Med. All right reserved. Source

Fan J.,Wuxi Childrens Hospital | Shi Y.,Wuxi Childrens Hospital | Cheng M.,Wuxi Childrens Hospital | Zhu X.,Wuxi Childrens Hospital | Wang D.,Wuxi Childrens Hospital
Journal of Paediatrics and Child Health

Aim: The aim of this study was to explore the efficacy and safety of treating idiopathic hypertrophic pyloric stenosis with sequential therapy (ST). Methods: From January 2010 to June 2013, 49 children with idiopathic hypertrophic pyloric stenosis were divided into two groups to accept either atropine ST (ST group, n = 26) or laparoscopic surgery (operation group, n = 23). The remission rate of vomiting, complications, hospital stay and medical expenditure were compared between the two groups. The body weight and the thickness of the pyloric muscle at 6 months after the treatments were also compared. Results: The remission rate of vomiting was lower in the ST group (88.5%; 23/26) than in the operation group (100%, 23/23). The difference in the incidence rate of complications, body weight and pyloric muscle thickness was not statistically significant between the two groups. However, the hospital stay was significantly longer, while the medical expenditure was significantly lower in the ST group than in the operation group. Conclusions: Atropine ST is safe, effective and cost-effective as compared with operation; however, the efficacy of ST is lower than operation. © 2016 Paediatrics and Child Health Division (The Royal Australasian College of Physicians) Source

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