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Wang W.-W.,Northwest University, China | He C.,Wuwei Academy of Forestry science | Cui J.,Northwest University, China | Wang H.-D.,Northwest University, China | Li M.-L.,Northwest University, China
Journal of Insect Science | Year: 2014

The diversity of the intestinal bacterial communities in Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae) larvae and adults was assayed by PCR-DGGE to determine whether different artificial diets could influence these bacterial communities. Two diets were used for feeding the larvae and four for the adults. Escherichia, Desemzia, Staphylococcus, Asticcacaulis, Cellvibrio, Aurantimonas, and Planomicrobium were isolated from the gut of the adults, with Escherichia and Staphylococcus being the main bacterial communities, and the quantities of intestinal bacterial were different in the adults fed different diets. Specifically, the amount of intestinal bacteria from the adults fed different diets had the following ranking according to the major component of the diet: ant powder > darkling beetle pupa powder > cricket powder > silkworm pupa powder. Escherichia, Bacillus, Staphylococcus, Kurthia, Planococcaceae, Ralstonia, Leptothrix, Acinetobacter, and Pseudomonas were isolated from the gut of the larvae. The quantity of intestinal bacteria from the larvae fed the darkling beetle pupae was greater than that from the larvae fed other artificial diets. This study, for the first time, investigated the effect of artificial diets on the bacterial community and the intestinal microbial diversity of D. helophoroides. Source

He C.,Northwest University, China | He C.,Wuwei Academy of Forestry science | Nan X.,Northwest University, China | Zhang Z.,Northwest University, China | Li M.,Northwest University, China
Journal of Insect Science | Year: 2013

The intestinal bacteria community structure and diversity of the Oriental armyworm, Mythimna separata (Walker) (Lepidoptera: Noctuidae), was studied by analysis of a 16S rDNA clone library, denaturing gradient gel electrophoresis, and culture-dependent techniques. The 16S rDNA clone library revealed a bacterial community diversity comprising Cyanobacteria, Firmicutes, Actinobacteria, Gracilicutes and Proteobacteria, among which Escherichia coli (Migula) (Enterobacteriales: Enterobacteriaceae) was the dominant bacteria. The intestinal bacteria isolated by PCR-denaturing gradient gel electrophoresis were classified to Firmicutes, Proteobacteria, and Gracilicutes, and E. coli was again the dominant bacteria. The culture-dependent technique showed that the intestinal bacteria belonged to Firmicutes and Actinobacteria, and Staphylococcus was the dominant bacteria. The intestinal bacteria of M. separata were widely distributed among the groups Cyanobacteria, Firmicutes, Actinobacteria, Gracilicutes, Proteobacteria, and Gracilicutes. 16S rDNA clone library, denaturing gradient gel electrophoresis, and culture-dependent techniques should be integrated to obtain precise results in terms of the microbial community and its diversity. © 2013 Journal of Insect Science. Source

Wang H.-D.,Northwest University, China | Li F.-F.,Northwest University, China | He C.,Wuwei Academy of Forestry science | Cui J.,Northwest University, China | And 2 more authors.
Journal of Insect Science | Year: 2014

The predatory beetle Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae) is a natural enemy of many longhorned beetles and is mainly distributed in both China and Japan. To date, no research on D. helophoroides P450 enzymes has been reported. In our study, for the better understanding of P450 enzymes in D. helophoroides, 100 novel cDNA fragments encoding cytochrome P450 were amplified from the total RNA of adult D. helophoroides abdomens using five pairs of degenerate primers designed according to the conserved amino acid sequences of the CYP6 family genes in insects through RT-PCR. The obtained nucleotide sequences were 250 bp, 270 bp, and 420 bp in length depending on different primers. Ninety-six fragments were determined to represent CYP6 genes, mainly from CYP6BK, CYP6BQ, and CYP6BR subfamilies, and four fragments were determined to represent CYP9 genes. Twenty-two fragments, submitted to GenBank, were selected for further homologous analysis, which revealed that some fragments of different sizes might be parts of the same P450 gene. © This is an open access paper. Source

Zhang Z.Q.,Northwest Agriculture and Forestry University | He C.,Wuwei Academy of Forestry science | Li M.L.,Northwest Agriculture and Forestry University
Journal of Insect Science | Year: 2014

Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE), and a culturedependent technique were used to study the diversity of the intestinal bacterial community in adult Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae). Universal bacterial primers targeting 200 bp regions of the 16S rDNA gene were used in the PCR-DGGE assay, and 14 bright bands were obtained. The intestinal bacteria detected by PCR-DGGE were classified to Enterococcus (Lactobacillales: Enterococcaceae), Bacillus (Bacillales: Bacillaceae), Cellvibrio (Pseudomonadales: Pseudomonadaceae), Caulobacter (Caulobacterales: Caulobacteraceae), and uncultured bacteria, whereas those isolated by the culture-dependent technique belonged to Staphylococcus (Bacillales: Staphylococcaceae), Pectobacterium Enterobacteriales: Enterobacteriaceae), and Enterobacter (Enterobacteriales: Enterobacteriaceae). These intestinal bacteria represented the groups Lactobacillales (Enterococcus), Pseudomonadales (Cellvibrio), Caulobacterales (Caulobacter), Bacilli (Bacillus and Staphylococcus), and Gammaproteobacteria (Pectobacterium and Enterobacter). Our results demonstrated that PCR-DGGE analysis and the culture-dependent technique were useful in determining the intestinal bacteria of D. helophoroides and the two methods should be integrated to characterize the microbial community and diversity. © 2014, Library of the University of Arizona. All rights reserved. Source

Meng R.,Northwest Agriculture and Forestry University | Qu D.,Northwest Agriculture and Forestry University | Liu Y.,Northwest Agriculture and Forestry University | Gao Z.,Northwest Agriculture and Forestry University | And 5 more authors.
Scientia Horticulturae | Year: 2015

We investigated anthocyanin concentrations by HPLC (high-performance liquid chromatography) and expression of MYB, bHLH and UFGT gene families by quantitative real time PCR in apple skin of red cultivar 'Starkrimson' and non-red cultivars 'Golden Delicious' and 'Granny Smith' in response to light. Young fruits were bagged at 40 DAFB (days after full bloom), and fruits of 'Golden Delicious' and 'Starkrimon' were debagged at 120 DAFB, while fruits of 'Granny Smith' at 160 DAFB. In bagged fruit, anthocyanin synthesis and related genes expression were both depressed, whereas these genes expression was enhanced by light, accompanied by anthocyanin accumulation in apple skin. 'Starkrimson' and 'Granny Smith' accumulated relatively higher anthocyanin concentrations than 'Golden Deliicous'. Transcript levels of most selected regulatory genes, including MdMYB1-1, MdMYB1-2, MdbHLH3-1 and MdbHLH33-1, were up-regulated in apple skin of all the three cultivars, although transcript levels of these genes in 'Starkrimson' and 'Granny Smith' increased before outer layered bag removal, and increased after outer layered in 'Golden Delicious'. However, UFGT family showed differences between red and non-red cultivars. MdUFGT2 was up-regulated only in non-red cultivars, while MdUFGT4 was up-regulated only in red skin cultivar, suggesting that UFGT is also important for anthocyanin accumulation in apple skin of different colors. Our results indicated that anthocyanin regulation in different colored apple cultivars were accomplished through different mechanisms, which will be valuable for further research of anthocyanin regulation in apple skin and for apple breeding programs that take genetic tools to improve the consistency of color in apples. © 2015 Elsevier B.V. Source

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