Wujiang Entry Exit Inspection and Quarantine Bureau


Wujiang Entry Exit Inspection and Quarantine Bureau

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Chang H.,Nanjing Forestry University | Hao D.-J.,Nanjing Forestry University | Yang X.-J.,Jiangsu Entry Exit Inspection and Quarantine Bureau | Xiao R.-T.,Wujiang Entry Exit Inspection and Quarantine Bureau | And 3 more authors.
Beijing Linye Daxue Xuebao/Journal of Beijing Forestry University | Year: 2013

Four methods used for extracting the genomic DNA of bark beetles, Xyleborus mutilatus (Coleoptera, Scolytidae) were studied in this article. An individual X. mutilatus beetle, a bark beetle leg and the specimen reserved in 75% ethanol were selected as testing sample respectively, the genomic DNA of bark beetle was extracted by using four different methods, including classical phenol/chloroform extracting method, improved cetyltrimethyl ammonium bromide (CTAB) method, the sodium dodecyl sulfate (SDS) method and animal tissue/cell genomic DNA extraction kit (i. e. paramagnetic particle method). The extracted genomic DNA was treated with photometric analysis and CO I gene amplification. The results showed that all the four methods can be used for the total DNA extraction. For the specimen soaked in 75% ethanol, these four methods were all applicable, however, only the paramagnetic particle method was suitable for the DNA extraction of the specimen, which had been soaked in 75% ethanol over two years. Furthermore, the paramagnetic particle method was also applicable for the total DNA extraction from microscale tissue. The study provids primary information for further molecular systematic research of bark beetles.

Chang H.,Nanjing Forestry University | Liu Q.,Nanjing Forestry University | Hao D.,Nanjing Forestry University | Liu Y.,Wujiang Entry Exit Inspection and Quarantine Bureau | And 3 more authors.
Mitochondrial DNA | Year: 2014

Members of the large and complex genus Xyleborus (Coleoptera: Scolytinae: Xyleborini) are the most commonly intercepted beetles associated with solid wood-packing materials at ports of entry in China. The accurate identification of species is critical in preventing the invasion of exotic insects. Considering the difficulties in morphological identification, genetic divergences of mitochondrial cytochrome c oxidase subunit I (COI) genes have been used in insect species identification. In this study, 32 Xyleborus and 2 outgroup species were collected from Jiangsu ports and selected to evaluate the effectiveness of DNA barcoding for Xyleborus species. The results showed that the mean interspecific divergence values (23.6%) were 15-fold higher than the observed intraspecific divergence (1.6%), except Xyleborus affinis. The results supported the inference that the barcode variation within species of insects is somewhat higher than interspecific ones. Thus, this study validated the effectiveness of barcoding for the identification of Xyleborus species. © 2014 Informa UK Ltd. All rights reserved.

Xu G.,Nanjing Agricultural University | Liu Y.,Wujiang Entry Exit Inspection and Quarantine Bureau | Chen S.,Nanjing Agricultural University | Chen F.,Nanjing Agricultural University
Russian Journal of Plant Physiology | Year: 2011

Disease assessment to measure severity of alternaria leaf spot (Alternaria tenuissima (Fr.) Wiltsh.) was performed in 32 wild species of Compositae family by seedling inoculation. It was found that two species were resistant, four were moderately resistant, and others were susceptible to various degrees. Some leaf morphological traits of two resistant and two highly susceptible wild species were studied. Trichome density and height and wax content were found to be associated with plant passive resistance, while stomata density was not associated. After A. tenuissima inoculation, enzymes phenylalanine ammonia-lyase, β-1,3-glucanase, and chitinase were also assayed. Their higher activities in the selected resistant wild species and lower activities in the highly susceptible ones were found. This suggests the possibility that these enzymes are not only constituents of resistance but also could be used as biochemical markers for screening Compositae wild species for alternaria leaf spot resistance. © 2011 Pleiades Publishing, Ltd.

Lv Y.,Wujiang Entry Exit Inspection and Quarantine Bureau | Lv Y.,Nanjing University | Zhang H.,Anhui University of Technology | Yao X.,Nanjing University | And 2 more authors.
Journal of Molecular Catalysis A: Chemical | Year: 2016

XRD, LRS, H2-TPR, and in situ FT-IR techniques were employed to investigate the dispersion, physicochemical behaviors of the Sm2O3 modification for CuO/γ-Al2O3 catalysts. And the catalytic performance for CuO/Sm2O3/γ-Al2O3 catalysts was tested by NO-CO reaction. The results indicated that (1) samarium oxide could be dispersed on the surface of γ-Al2O3 with a dispersion capacity of about 0.625 mmol/100 m2 and the supported copper oxide could be also dispersed upon the surface of Sm2O3/γ-Al2O3; (2) the reducibility of copper oxide was inhibited by the presence of samarium oxide, which could be influenced by the loading amounts of Sm2O3 upon γ-Al2O3 surface; (3) FT-IR results implied that the presence of samarium oxide species made the copper oxide hard to be reduced by CO while enhanced the NO strongly adsorbed; (4) NO-CO reaction could start to react only when both of them were in adsorbed states and the formation of free-like NO3 - species was recognized as the intermediates of the reaction; (5) the reaction activity was negatively influenced by the introduction of samarium oxide in CuO/γ-Al2O3 catalysts. And a possible reaction pathway was tentatively proposed to discuss the NO-CO reaction based on all of these results. © 2016 Elsevier B.V. All rights reserved.

Liu Y.-J.,Wujiang Entry Exit Inspection and Quarantine Bureau | Liu Y.-J.,Jiangsu University | Liu Y.-J.,Central South University | Song Y.,Wujiang Entry Exit Inspection and Quarantine Bureau | And 6 more authors.
Zhongguo Youse Jinshu Xuebao/Chinese Journal of Nonferrous Metals | Year: 2011

The power battery was manufactured with the commercial LiMn 2O 4 and graphite. The storage performance of LiMn 2O 4 battery was tested. The cathode, anode and electrolyte before and after storage were characterized. The result shows that the capacity fading ratio of LiMn 2O 4 battery at half-charged state is 3.7%, but the cycling performance is improved after storage. XRD results show that the crystal lattice of LiMn 2O 4 shrinks. TEM results show that the surface of LiMn 2O 4 particles is covered with SEI film. The cathode impedance increases from 62.69 Ω to 84.64 Ω and that of anode increases from 183.1 Ω to 301 Ω. FT-IR results show that the solvent and solutes of electrolyte decompose at a certain degree after storage. The polarization, Mn dissolution, electrolyte decomposition and the incrassation of anode SEI are responsible for the capacity fading of LiMn 2O 4 battery after storage.

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