Wuhan Women and Children Health Care Center

Wuhan, China

Wuhan Women and Children Health Care Center

Wuhan, China
SEARCH FILTERS
Time filter
Source Type

Zhang P.,Wuhan Women and Children Health Care Center | Lu X.,Huazhong University of Science and Technology | Chen J.,Huazhong University of Science and Technology | Chen Z.,Huazhong University of Science and Technology
Neural Regeneration Research | Year: 2014

Artifcial guidance channels containing Schwann cells can promote the regeneration of injured peripheral nerve over long distances. However, primary Schwann cells are not suitable for autotransplantation. Under specifc conditions, skin-derived progenitors can be induced to differentiate into Schwann cells. Therefore, adult rat dorsal skin (dermis)-derived progenitors were isolated and induced to differentiate with DMEM/F12 containing B27, neuregulin 1, and for-skolin. Immunofuorescence staining and reverse transcription polymerase chain reaction (RT-PCR) confrmed that the resultant cells were indeed Schwann cells. Artifcial guidance channels containing skin-derived progenitors, Schwann cells originating from skin-derived progenitors, or primary Schwann cells were used to bridge 5 mm sciatic nerve defects. Schwann cells originating from skin-derived progenitors signifcantly promoted sciatic nerve axonal regeneration. The signifcant recovery of injured rat sciatic nerve function after the transplantation of Schwann cells originating from skin-derived progenitors was confrmed by electromyogram. The therapeutic effect of Schwann cells originating from skin-derived progenitors was better than that of skin-derived progenitors. These findings indicate that Schwann cells originating from skin-derived precursors can promote peripheral nerve regeneration in rats. © 2014, Editorial Board of Neural Regeneration Research. All right reserved.


PubMed | Wuhan Women and Children Health Care Center and Huazhong University of Science and Technology
Type: Journal Article | Journal: PloS one | Year: 2015

To assess whether pre-pregnancy body mass index (BMI) modify the relationship between gestational weight gain (GWG) and child birth weight (specifically, presence or absence of low birth weight (LBW) or presence of absence of macrosomia), and estimates of the relative risk of macrosomia and LBW based on pre-pregnancy BMI were controlled in Wuhan, China.From June 30, 2011 to June 30, 2013. All data was collected and available from the perinatal health care system. Logistic regression models were used to estimate the independent association among pregnancy weight gain, LBW, normal birth weight, and macrosomia within different pre-pregnancy BMI groups. We built different logistic models for the 2009 Institute of Medicine (IOM) Guidelines and Chinese-recommended GWG which was made from this sample. The Chinese-recommended GWG was derived from the quartile values (25th-75th percentiles) of weight gain at the time of delivery in the subjects which comprised our sample.For LBW children, using the recommended weight gain of the IOM and Chinese women as a reference, the OR for a pregnancy weight gain below recommendations resulted in a positive relationship for lean and normal weight women, but not for overweight and obese women. For macrosomia, considering the IOMs recommended weight gain as a reference, the OR magnitude for pregnancy weight gain above recommendations resulted in a positive correlation for all women. The OR for a pregnancy weight gain below recommendations resulted in a negative relationship for normal BMI and lean women, but not for overweight and obese women based on the IOM recommendations, significant based on the recommended pregnancy weight gain for Chinese women. Of normal weight children, 56.6% were above the GWG based on IOM recommendations, but 26.97% of normal weight children were above the GWG based on Chinese recommendations.A GWG above IOM recommendations might not be helpful for Chinese women. We need unified criteria to classify adult BMI and to expand the sample size to improve representation and to elucidate the relationship between GWG and related outcomes for developing a Chinese GWG recommendation.


PubMed | Wuhan Women and Children Health Care Center, Nanjing Medical University, Shanghai JiaoTong University and Chengdu Womens and Childrens Central Hospital
Type: Journal Article | Journal: Clinical pediatrics | Year: 2016

Objective This study aims to analyze the clinical and endoscopic presentations of upper gastrointestinal bleeding (UGIB) in the Chinese children. Methods A 10-year retrospective study was made on children with UGIB and undertaken esophagogastroduodenoscopy (EGD) from 4 tertiary referral centers in China. Results Of the 1218 children studied, the bleeding source was found in 76.4%. Erosive gastritis was the most common endoscopic finding (33.5%), followed by duodenal ulcer (23.2%). The proportion of erosive gastritis decreased with age (correlation coefficient = -0.787), and duodenal ulcer increased with age (correlation coefficient = 0.958). The bleeding source was more likely to be determined in children having EGDs within 48 hours (80.6% vs 67.9%). Conclusions In Chinese children with UGIB, erosive gastritis and duodenal ulcer were the leading causes, and their proportions varied with age. EGDs performed within 48 hours may improve the possibility of finding the source of bleeding.


PubMed | Wuhan Women and Children Health Care Center, Wuhan Environmental Monitoring Center, Yale University, Saint Louis University and Huazhong University of Science and Technology
Type: Journal Article | Journal: Journal of exposure science & environmental epidemiology | Year: 2016

Maternal exposure to ambient air pollution has increasingly been linked to congenital heart defects (CHDs). The objective of this study was to evaluate whether high levels of maternal exposure to PM2.5 and PM10 are related to increased risk of CHDs in Wuhan, China. We conducted a cohort study with a total of 105,988 live-born infants, stillbirths, and fetal deaths. The study included mothers living in the urban district of Wuhan during pregnancy over the 2-year period from 10 June 2011 to 9 June 2013. For each study participant, we assigned 1-month and 1-week averages of PM10 and PM2.5 exposure based on measurements obtained from the nearest exposure monitor to the living residence of mothers during their early pregnancy period. Logistic regression analyses were conducted to calculate the adjusted odds ratios (aORs) and 95% confidence intervals (CI) for the association between exposure to these ambient air pollutants during early pregnancy and CHDs. We observed an increased risk of CHDs, particularly ventricular septal defect (VSD), with increasing PM2.5 exposure. Using 1-week averages, we also observed significant monotonically increasing associations between PM2.5 exposure during weeks 7-10 of pregnancy and risk of VSD, with aORs ranging from 1.11 to 1.17 (95% CI: 1.02-1.20, 1.03-1.22, 1.05-1.24, and 1.08-1.26 separately) per a 10g/m(3) change in PM2.5 concentration. Our study contributes to the small body of knowledge regarding the association between in utero exposure to air pollution and CHDs, but confirmation of these associations will be needed in future studies.


He L.,Wuhan University | He L.,Peoples Hospital of Tongcheng County | Xia D.,Wuhan University | Wu J.,Wuhan Women and Children Health Care Center
Medical Journal of Wuhan University | Year: 2016

Objective: To observe the effect of ibuprofen on Fos expression and inflammatory states in rat spinal cord after formalin stimulation. Methods: SD rats were randomly divided into blank control group (A), saline injection control group (group B), low-dose ibuprofen group (C1), middle-dose ibuprofen group (C2 group), and high-dose ibuprofen group (C3 group), with six rats in each group. After formalin stimulation for 180 min, the rat spinal cord neurons Fos protein expression was detected by immunohistochemical staining, and the mechanical allodynia behavior threshold was determined. Results: Ibuprofen treatment significantly increased the mechanical allodynia threshold in a dose-dependent manner (P<0.05). Immunohistochemical results showed that Fos-positive neurons were more in A and B groups than ibuprofen treated groups, and ibuprofen also showed a dose-dependent inhibitory effect. Conclusion: Ibuprofen increases noxious stimuli threshold and inhibits Fos-positive neurons maybe through inhibiting prostaglandin E synthesis in vivo. © 2016, Wuhan University of Technology. All right reserved.


Lu X.-X.,Wuhan Women and Children Health Care Center | McCoy K.S.,Ohio State University | Hu W.-K.,Huazhong University of Science and Technology | Xu J.-L.,Wuhan Women and Children Health Care Center | And 3 more authors.
Journal of Huazhong University of Science and Technology - Medical Science | Year: 2013

This study investigated the expression of interleukin-17 (IL-17) and T cell immunoglobulin mucin and domain-containing molecule-3 (Tim-3) in bronchoalveolar lavage fluid (BALF) of asthmatic mice and the effect of dexamethasone (DEX) on these factors. Thirty-six mice were randomly divided into three groups: normal group, asthmatic group and DEX group. The mouse model of asthma was established by sensitization with ovalbumin in both the asthmatic and DEX groups. The levels of IL-6, IL-10, IL-17 and TGF-β were measured in BALF by enzyme-linked immunesorbent assay (ELISA). The mRNA expression level of Tim-3 was detected by reverse transcription polymerase chain reaction (RT-PCR). The ratio of Tim-3+CD4+ cells to total CD4+ cells in BALF was determined by flow cytometry. Differential inflammatory cells in BALF were detected. The correlations among IL-17, IL-6, IL-10, Tim-3 and inflammatory cells were analyzed. The results showed that the levels of IL-17, IL-6 and Tim-3 were substantially increased and the IL-10 level decreased in BALF in the asthmatic mice, which was significantly reversed by DEX treatment. IL-17 expression was positively correlated with IL-6 and Tim-3 expression and the number of inflammatory cells but negatively with IL-10 expression. These results indicate that the increased expression of IL-17 and Tim-3 in BALF may be implicated in the occurrence and development of asthmatic inflammation; the mechanism by which DEX suppresses asthmatic airway inflammation involves down-regulation of IL-17 and Tim-3 levels. © 2013 Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg.


Zhang B.,Wuhan Women and Children Health Care Center
Journal of Exposure Science and Environmental Epidemiology | Year: 2016

Maternal exposure to ambient air pollution has increasingly been linked to congenital heart defects (CHDs). The objective of this study was to evaluate whether high levels of maternal exposure to PM2.5 and PM10 are related to increased risk of CHDs in Wuhan, China. We conducted a cohort study with a total of 105,988 live-born infants, stillbirths, and fetal deaths. The study included mothers living in the urban district of Wuhan during pregnancy over the 2-year period from 10 June 2011 to 9 June 2013. For each study participant, we assigned 1-month and 1-week averages of PM10 and PM2.5 exposure based on measurements obtained from the nearest exposure monitor to the living residence of mothers during their early pregnancy period. Logistic regression analyses were conducted to calculate the adjusted odds ratios (aORs) and 95% confidence intervals (CI) for the association between exposure to these ambient air pollutants during early pregnancy and CHDs. We observed an increased risk of CHDs, particularly ventricular septal defect (VSD), with increasing PM2.5 exposure. Using 1-week averages, we also observed significant monotonically increasing associations between PM2.5 exposure during weeks 7–10 of pregnancy and risk of VSD, with aORs ranging from 1.11 to 1.17 (95% CI: 1.02–1.20, 1.03–1.22, 1.05–1.24, and 1.08–1.26 separately) per a 10 μg/m3 change in PM2.5 concentration. Our study contributes to the small body of knowledge regarding the association between in utero exposure to air pollution and CHDs, but confirmation of these associations will be needed in future studies.Journal of Exposure Science and Environmental Epidemiology advance online publication, 17 February 2016; doi:10.1038/jes.2016.1. © 2016 Nature America, Inc.


Lu X.-X.,Wuhan Women and Children Health Care Center | McCoy K.S.,Ohio State University | Xu J.-L.,Wuhan Women and Children Health Care Center | Hu W.-K.,Huazhong University of Science and Technology | Chen H.-B.,Wuhan Women and Children Health Care Center
Inflammation | Year: 2013

Since CD4+ T cells play a pivotal role in the development of airway inflammation and hyperresponsiveness, targeting activated CD4+ T cell subsets and increasing the cells with regulatory function would be a logical therapeutic approach. We showed that this outcome can be achieved by local therapy with Tim-3, which is a negative regulator of CD4+ T cells. Tim-3 expression was up-regulated by ovalbumin (OVA) induction. Attenuating Tim-3 expression by RNA interference suppressed allergen-induced immune responses. Intranasal application of Tim-3 shRNA diminished airway inflammation and hyperresponsiveness. Multiple mechanisms were involved in the inhibitory effects, including regulation the imbalance of Th1/Th17 and increasing Treg cell expression. Our results indicate that the Tim-3 pathway is highly involved in the regulation of asthma. Targeting Tim-3 by siRNA may hold therapeutic potential in preventing the development of allergic asthma. © 2012 Springer Science+Business Media New York.


Li D.,Wuhan Women and Children Health Care Center
Medical Journal of Wuhan University | Year: 2015

Objective: To observe clinical effect of pediatric adenoidectomy under naso-antral endoscopy. Methods: Clinical data of 439 patients with pediatric adenoidectomy under naso-antral endoscopy were retrospectively analyzed and compared with the clinical data of 420 patients with conservative treatment. Clinical effect, symptoms, and signs changes were compared, and surgical complications were recorded. Results: Treatment efficiency of observation group with the rate of 86.6%(380/439) was significantly higher than that of control group whose rate was 38.6% (162/420). Complication rate, 32.8% (42/128) of observation group was significantly lower than that of control group, 48.3% (42/120). Clinical signs and symptoms such as snoring, mouth breathing, sleep apnea, secretory otitis media and developmental disorders were significantly lower than those of control group. The differences were statistically significant (P<0.05). There were no such complications as soft palate and eustachian tube damage in control group. Seven cases (1.6%) of postoperative bleeding in patients were treated by electric coagulation hemostasis. Conclusion: Clinical effect of pediatric adenoidectomy under naso-antral endoscopy is significant, with comprehensive improvement on clinical symptoms and signs and fewer complications. ©, 2015, Editorial Board of Medical Journal of Wuhan University. All right reserved.


PubMed | Wuhan Women and Children Health Care Center and Huazhong University of Science and Technology
Type: Journal Article | Journal: Neural regeneration research | Year: 2014

Artificial guidance channels containing Schwann cells can promote the regeneration of injured peripheral nerve over long distances. However, primary Schwann cells are not suitable for autotransplantation. Under specific conditions, skin-derived progenitors can be induced to differentiate into Schwann cells. Therefore, adult rat dorsal skin (dermis)-derived progenitors were isolated and induced to differentiate with DMEM/F12 containing B27, neuregulin 1, and forskolin. Immunofluorescence staining and reverse transcription polymerase chain reaction (RT-PCR) confirmed that the resultant cells were indeed Schwann cells. Artificial guidance channels containing skin-derived progenitors, Schwann cells originating from skin-derived progenitors, or primary Schwann cells were used to bridge 5 mm sciatic nerve defects. Schwann cells originating from skin-derived progenitors significantly promoted sciatic nerve axonal regeneration. The significant recovery of injured rat sciatic nerve function after the transplantation of Schwann cells originating from skin-derived progenitors was confirmed by electromyogram. The therapeutic effect of Schwann cells originating from skin-derived progenitors was better than that of skin-derived progenitors. These findings indicate that Schwann cells originating from skin-derived precursors can promote peripheral nerve regeneration in rats.

Loading Wuhan Women and Children Health Care Center collaborators
Loading Wuhan Women and Children Health Care Center collaborators