Shen H.,Wuhan University |
Li W.,Wuhan University |
Tian Y.,Wuhan University |
Xu P.,Wuhan Union Hospital |
And 3 more authors.
Journal of Cellular Biochemistry | Year: 2015
The dysregulation of microRNAs (miRNAs) contributes to the pathogenesis of human malignancies, and miRNA expression can be affected by genetic and epigenetic changes, such as methylation of the CpG islands of their promoters. To identify miRNAs regulated by DNA methylation, the global miRNA expression profile was analyzed in two hepatocellular carcinoma (HCC) cell lines and two normal immortalized cell lines treated with 5-Aza-2′-deoxycytidine (DAC, an inhibitor of DNA methylation) plus TSA (Trichostatin A, histone deacetylase inhibitor). Results revealed that these epigenetic drugs differentially affect miRNA expression that is dependent or independent of cell type, especially miR-362-3p. miR-362-3p expression increased while methylation of its promoter significantly decreased in human HCC cells and tissues compared with normal cells and adjacent noncancerous tissues. Ectopic expression of miR-362-3p increased proliferation and anchorage-independent soft agar growth and its expression inhibition had opposing effects that were associated with regulation of its direct target - Tob2 in HCC cells. Inhibition of Tob2 recapitulated the effects of miR-362-3p overexpression, whereas enforced Tob2 expression reversed the promoting effects of miR-362-3p. Tob2 expression was reduced in human primary HCCs compared to adjacent noncancerous tissues. Our findings suggest that dysregulation of miR-362-3p and Tob2 may contribute to HCC malignancy. J. Cell. Biochem. 116: 1563-1573, 2015. © 2015 Wiley Periodicals, Inc. © 2015 Wiley Periodicals, Inc.
Liraglutide provides similar glycaemic control as glimepiride (both in combination with metformin) and reduces body weight and systolic blood pressure in Asian population with type 2 diabetes from China, South Korea and India: A 16-week, randomized, double-blind, active control trial
Yang W.,China Japan Friendship Hospital |
Chen L.,Wuhan Union Hospital |
Ji Q.,Xijing University |
Liu X.,Harbin Medical University |
And 8 more authors.
Diabetes, Obesity and Metabolism | Year: 2011
Aim: To assess and compare the efficacy and safety of liraglutide with those of glimepiride, both in combination with metformin for the treatment of type 2 diabetes in Asian population from China, South Korea and India. Methods: A 16-week, randomized, double-blind, double-dummy, four-arm, active control trial was carried out. In total, 929 subjects with type 2 diabetes with a mean (±s.d.) age of 53.3 ± 9.5 years, HbA1c of 8.6 ± 1.0% and body weight of 68.1 ± 11.7 kg were randomized (liraglutide 0.6, 1.2 or 1.8 mg once daily or glimepiride 4 mg once daily all in combination with metformin: 1 1 1 1). One subject withdrew immediately after randomization and before exposure. Results: HbA1c was significantly reduced in all groups compared with baseline. Treatment with liraglutide 1.2 and 1.8 mg was non-inferior to glimepiride (mean HbA1c reduction: 1.36% points, 1.45% points and 1.39% points, respectively). No significant difference was shown in the percentage of subjects reaching American Diabetes Association HbA1c target <7% or American Association of Clinical Endocrinologists target ≤6.5% between liraglutide 1.2 and 1.8 mg and glimepiride. Liraglutide was associated with a 1.8-2.4 kg mean weight reduction, compared with a 0.1 kg mean weight gain with glimepiride. Liraglutide led to a significantly greater reduction in systolic blood pressure (SBP) compared with glimepiride. Two subjects in the glimepiride group reported major hypoglycaemia while none in the liraglutide groups. Liraglutide was associated with about 10-fold lower incidence of minor hypoglycaemia than glimepiride. Gastrointestinal disorders were the most common adverse events (AEs) for liraglutide, but were transient and resulted in few withdrawals. Conclusions: In Asian subjects with type 2 diabetes, once-daily liraglutide led to improvement in glycaemic control similar to that with glimepiride but with less frequent major and minor hypoglycaemia. Liraglutide also induced a significant weight loss and reduced SBP and was generally well tolerated. The most frequently reported AE was transient nausea. The effect of liraglutide in this Asian population is comparable to the effects seen in Caucasian, African American and Hispanic populations in global liraglutide phase 3 trials. © 2010 Blackwell Publishing Ltd.
Qin C.,Wuhan Polytechnic University |
Feng X.,Wuhan Union Hospital
Advanced Materials Research | Year: 2012
Nurses in Department of Emergency Medicine face the emergency of special populations; they live in special circumstances and shoulder a special mission, under the dual stress of internal and external environment source. However, whether the stressors cause stress to nurses is depending on the individual needs of the environment and the results interacting with their own resources assessment, if the assessment that the stress source exceeds the individual's response resources, the stress will be generated, which threats to the physical and mental health. This article pointed out that the stress of nurses in Department of Emergency Medicine is mainly from the patient care and nursing, most nurses are in moderate stress levels, nurses mainly use problem-directed response method, rather than emotion-directed response method. The problem-directed response method is significant negative correlation to the stress and emotion-directed response method is significant positive correlation. © (2012) Trans Tech Publications, Switzerland.
Lu L.,Wuhan University |
Han H.,Wuhan University |
Tian Y.,Wuhan University |
Li W.,Wuhan University |
And 3 more authors.
Molecular Carcinogenesis | Year: 2015
Dysregulation of c-Myc (Myc) has been shown to contribute to progression of hepatocellular carcinoma, however, the detailed molecular mechanism remains poorly understood. Here, we report that Myc binds to the Aurora kinase A (Aurka) promoter and induces expression of Aurka in HCC cells. Increased expression of Aurka correlates with that of Myc in HCC. Nuclear accumulation of Aurka was confirmed by subcellular protein fractionation and immunoblot experiments in HCC cells. Myc inhibition decreases the nuclear accumulation of Aurka in HCC cells. Also Aurka accumulating in the nucleus up-regulates Myc transcription by binding the Myc promoter containing the highly conserved CCCTCCCCA in the NHE region of the CpG islands. Inhibition of Myc or Aurka diminishes the malignant phenotypes of HCC cells by down-regulating some common target genes. Also Aurka and Myc mediates the effects of each other, at least partially, on proliferation, anchorage-independent soft agar growth, and ATP production. Blocking Aurka in an orthotopic model significantly impairs tumor growth in mice. These results identify a Myc-Aurka feedback loop in which Myc and Aurka regulate expression of each other at the transcriptional level and both play an important role in hepatocarcinogenesis. © 2014 Wiley Periodicals, Inc.
Ma L.,Fudan University |
Xiang L.-H.,Fudan University |
Yu B.,Peking University |
Yin R.,Chongqing Medical University |
And 12 more authors.
Photodiagnosis and Photodynamic Therapy | Year: 2013
Objectives: To investigate the efficacy and safety of low-concentration 5-aminolevulinic acid photodynamic therapy (ALA-PDT) in the treatment of different severity of acne vulgaris and optimize the treatment regimen. Methods: A self-controlled multicenter clinical trial was carried out in 15 centers throughout China. A total of 397 acne patients of grade II-IV received 3- or 4-session PDT treatment. 5% ALA gel was applied topically to acne lesions for 1h incubation. The lesions were irradiated by a LED light of 633nm at dose levels of 96-120J/cm2. Clinical assessment was conducted before and after every treatment up to 8 weeks. Results: The effective rate overall and of grade II, III and IV are 82.1%, 71.6%, 79.6% and 88.2%, respectively. The effective rate rises significantly proportionally to the severity of acne (P<. 0.01). No significant differences are found in the efficacy between patients received 3-session and 4-session PDT treatments (P>. 0.05). The count of inflammatory and non-inflammatory acne lesions gradually decrease after each treatment (P<. 0.01) and during the 8-week follow up (P<. 0.01 or P<. 0.05). Maximum efficacy is obtained at 8 weeks after the treatment completion. Conclusions: A low-dose topical ALA-PDT regimen using 5% ALA, 1. h incubation and red light source of 3 treatment sessions is suggested as optimal scheme for the treatment of different severity of acne vulgaris in Chinese patients. Superior efficacy is found in severe cystic acne of grade IV with mild side effects. © 2013 Elsevier B.V..