Wuhan Research Center on Quality Control Engineering of Bee Products

Wuhan, China

Wuhan Research Center on Quality Control Engineering of Bee Products

Wuhan, China
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Zhu X.,Huazhong Agricultural University | Zhu X.,Wuhan Research Center on Quality Control Engineering of Bee Products | Ye F.,Huazhong Agricultural University | Ye F.,Wuhan Research Center on Quality Control Engineering of Bee Products | And 7 more authors.
Chinese Journal of Chromatography (Se Pu) | Year: 2010

A method for the simultaneous determination of 5 organic acids (L-malic acid, male-ic acid, succinic acid, citric acid, D-malic acid) in honey was developed by solid-phase extraction and high performance liquid chromatography (SPE-HPLC). The sample was purified by a Bond Elutes SAX SPE cartridge after pretreatment, then separated using a reversed-phase C18-MS- E column. A 2% metaphosphoric acid solution was used as the mobile phase at a flow rate of 0. 7 mL/min. The detection was performed by a diode array detector at 210 nm. Under these conditions; the linear ranges of the method for L-malic acid was 5.0 -500 mg/L, for maleic acid was 0. 1 -500 mg/L, and for succinic acid, citric acid, D-malic acid were 18.0 -500 mg/L. The correlation coefficients of 5 organic acids were more than 0. 996 7. The average recoveries of 5 organic acids were 86. 0% - 103. 9% with the relative standard deviations of 5. 7% - 9. 8% (n =6/. The detection limits ranged from 0. 06 to 9. 4 mg/kg. The method can be used for determining organic acids in honey samples.

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