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Wuhan, China

Objective: To explore the combined influence of deproteinized extract of calf blood and ultrasonic atomization of methylcobalamin on corneal nerve recovery after LASIK. Methods: A total of 78 cases (146 eyes) undergone LASIK operation were treated with deproteinized extract of calf blood with or without ultrasonic atomization of methylcobalamin for three months. The corneal indicators (central perception, nerve fiber density and endothelial count), general indicators (vision, tear film breakup time, Schirmer's test and aqueous flare in anterior chamber), and biochemical indicators of tear (TNF-α, IL-2 and NGF) were followed up for six months. Results: Compared with that respectively in control group, the aqueous flare, TNF-α, IL-2, and NGF were improved in the combined treatment group one week after operation; after one month, central perception, nerve fiber density, vision, tear film breakup time, flash and NGF indicators showed significant improvement; after 6 months' follow-up, nerve fiber density, tear film breakup time, Schirmer and flash indicators were better in combined treatment group. Conclusion: The combined treatment of deproteinized extract of calf blood and ultrasonic atomization of methylcobalamin can accelerate the corneal nerve repair after LASIK, and promote the recovery of visual acuity and tear film stability, maybe through inhibiting inflammation and inducing NGF secretion.

He C.Z.,Wuhan Puai Hospital
Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics | Year: 2011

To study the effects of astragaloside on the expression of insulin-like growth factor-1 (IGF-1) and associated proteins in mice with viral myocarditis. Sixty-five 4-week-old BALB/C mice were randomly divided into 5 groups: normal control, astragaloside control, untreated myocarditis, low-dose and high-dose astragaloside-treated myocarditis. The BALB/C mice in the later three groups were intraperitoneally injected with CVB3. The low-dose and high-dose astragaloside-treated myocarditis groups were given astragaloside of 0.07 and 0.6 mg/kg•d, respectively by intragastric administration. Fifteen days later, the samples of blood and muscular tissues were obtained. The expression of IGF-1 in plasma was measured using ELISA. The levels of IGF-1 and associated proteins in muscular tissues were measured by immunohistochemistry. The expression of IGF-1 mRNA in muscular tissues was examined by RT-polymerase chain reaction (RT-PCR). The expression of IGF-1 and associated proteins increased significantly in mice infected with CVB3. High-dose astragaloside treatment reduced the expression of IGF-1 and associated proteins, but low-dose astragaloside did not. High-dose astragaloside may reduce the expression of IGF-1 and associated proteins in mice with acute viral myocarditis, possibly thus providing protective effects on muscular tissues.

Ke J.,Wuhan Puai Hospital
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2013

To compare the efficacy and safety of applications of GlideScope video laryngoscope (GSVL) and Macintosh direct laryngoscope (MDLS) during endotracheal intubation under general anesthesia of abdominal snoring surgeries. Forty patients ranged from American Society of Anesthesiologists I to II scheduled for snoring surgery under general anesthesia with endotracheal intubation were randomly divided into GSVL group and MDLS group (20 cases in each group), After traditional induction, endotracheal intubation (ETT) performed by GSVL or MDLS. Glottic exposure, the time of endotracheal intubation(from mask ventilation ending to successful intubation), the times of tracheal intubation operation were recorded in both groups. The mean artery pressure(MAP), heart rate(HR) were monitored before induction (basic value T0), before tracheal intubation (T1), after tracheal intubation (T2), 1 min after tracheal intubation (T3), 3 mins after tracheal intubation (T4). The glottic exposure of GSVL group is superior to that of MDLS group. There was no significant difference in the time of tracheal intubation between MDLS group (27 +/- 11)s and GSVL group (26 +/- 11)s (P < 0.05). Compared with T0, MAP of T1 was lower in two groups (P < 0.05), while HR were similar both in two groups (P > 0.05). MAP of T2 and T3 increased and HR became faster (P < 0.05). MAP of T4 declined, but HR still increased (P < 0.05). Between the two groups, there was no significant difference of MAP or HR (P > 0.05). Compared with MDLS,the GSVL is of benefit to improve the glottic exposure in endotracheal intubation to the snoring patients,so as to improve the success rate of tracheal intubation. But there is no obvious advantages via GSVL in preventing hemodynamic reaction during tracheal intubation.

Ke J.,Wuhan Puai Hospital
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2013

To observe the variance of blood pressure (BP) after controlled hypotension in endoscopic sinus surgery with dexmedetomidine. Sixty patients undergoing elective endoscopic sinus surgery were randomly assigned to receive dexmedetomidine (Group D) or physiological saline (Group S). For the two groups, after the same induction of anesthesia procedure, Group D were injected with dexmedetomidine with 0.8 microg/kg of priming dose in 10 min and 0.5 microg/(kg x h) of maintenance dose i. v. drip for 30 min. The Group S were injected with physiological saline in the dose the same as Group D. The measurement of the mean aortic pressure(MAP), heart rate (HR) and the colouration of the Nasal packing material and exudation were taken at the end of the surgery (T1), at the time of extubation (T2), 10 min after extubation (T3), 30 min after extubation (T4), 2 h after extubation (T5), and 4 h after extubation (T6). The MAP and HR of Group S at the points T2-T6 were significantly higher compared with those at the point T1 (P < 0.05). The MAP and HR of Group D at each time point did not change significantly (P > 0.05). The MAP and HR of Group S at each time point were higher compared with Group D (P < 0.05). The number of cases with postoperative nasal exudate in Group S was significantly more than in Group D (27:18) (P < 0.05). The perioperative dexmedetomidine medication can significantly reduce fluctuations in BP in endoscopic sinus surgery, so that to stable hemodynamics and reduce nasal bleeding.

Hu B.,Hubei University | Xiang H.,Hubei University | Liang H.,Hubei University | Yu L.,Wuhan Central Hospital | And 4 more authors.
Chinese Medical Journal | Year: 2013

Background Central venous pressure (CVP) and intrathoracic blood volume index (ITBVI) were used to assess the fluid status. It has previously been shown that CVP is not as accurate as ITBVI for all the shock patients. We therefore hypothesized that the change of CVP has the ability to predict fluid responsiveness in some clinical cases of shock. Methods From September 1st 2009 to September 1st 2011, sixty-three patients with shock from different Intensive Care Unit (ICU) were collected into this retrospective study. All the patients received fluid challenge strategy (infusing 300 ml hydroxyethyl starch in 20 minutes), were monitored with CVP and pulse-indicated continuous cardiac output (PICCO). The correlation between changes in cardiac index (δCI), CVP (δCVP) and ITBVI (δITBVI) were analyzed. Fluid responsiveness was defined as an increase in CI ≥10%. Receiver operating characteristic (ROC) curves were generated for δCVP and δITBVI. Results For all the patients, there was no correlation between δCI and δCVP (P=0.073), but in the subgroup analysis, the correlation between δCI and δCVP was significant in those younger than 60 years old (P=0.018) and those with hypovolemic shock (P=0.001). The difference of areas under the ROC curves of δCVP and δITBVI were not statistically significant in the group younger than 60 years old or hypovolemic shock group (P >0.05, respectively). However, no similar results can be found in the group older than 60 years old and the other two shock type groups from ROC curves of δCVP and δITBVI. Conclusions δCVP is not suitable for evaluating the volume status of the shock patients with fluid resuscitation regardless of their condition. However, in some ways, δCVP have the ability to predict fluid responsiveness in the younger shock patients or in the hypovolemic shock patients.

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