Chen Q.-X.,Jianghan University |
Liang B.,Huazhong Agricultural University |
Li Y.,Huazhong Agricultural University |
Peng Y.-S.,Wuhan Product Quality Supervision and Inspection Institute |
And 3 more authors.
Modern Food Science and Technology | Year: 2014
The effect of polysaccharides with low molecule weight from Agaricus blazei Murrill (LABP) on proliferation and invasion in gastric cancer cell line BGC823 was observed by WST-1 and Transwell assay, and the expression changes of some related molecules in proteinic level were determined by Western blot, to explore the mechanism of LABP affected on BGC823 cells. Compared with the control group, LABP group cells were significantly declined (P<0.05) in a dose-dependent manner. BGC823 cell inhibition rates by LABP were 33.4% and 69.8%, respectively on LABP concentration of 1 × 10-4g/mL. The cells permeated the membrane of LABP groups were obviously less and the protein expression of Caspase 3 of LABP group was dramatic increased (P<0.05). The expression of MMP 9 was dramatic down-regulated (P<0.05), while E-cadherion had no significant difference (P>0.05). Thus, low-molecule polysaccharide of Agaricus blazei Murrill (LABP) had a dramatic inhibiting effect on growth and invasion in gastric cancer BGC823 cells. LABP inhibited the gastric cancer BGC823 cells growth, possibly by up-regulating the protein expression of Caspase 3 and down-regulating MMP 9.
Fang H.,Wuhan Product Quality Supervision and Inspection Institute |
Zhou Y.,Wuhan Product Quality Supervision and Inspection Institute |
Lu Y.,Wuhan Product Quality Supervision and Inspection Institute |
Jiang X.,Wuhan Product Quality Supervision and Inspection Institute |
Yang Y.,Wuhan Product Quality Supervision and Inspection Institute
Chinese Journal of Chromatography (Se Pu) | Year: 2012
An accurate determination of quantitative and confirmative method for sodium cyclamate in liquor by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with linear trap technology has been established. Without pretreatment, the sample was directly injected after filtering through a 0.2 μm micro filter. The HPLS separation was performed on an Atlantis dC 18 column (150 mm × 2.1 mm, 3 μm) by gradient elution with methanol and water containing 0.1% (v/v) formic acid. The eluent was determined and confirmed in multiple reaction monitoring-enhanced production (MRM-EPI) scan mode. The acquired data from MRM for the quantitative determination, and the production spectra were used for library search for qualitative confirmatory analysis. External standard was used for the quantitative determination of sodium cyclamate in liquor, and good linearity (r=0.9991) was obtained over the range of 1.320-132.0 μg/L. The limit of detection (LOD, S/N=3) for sodium cyclamate was 0.1 μg/L. The average recoveries ranged from 96.38% to 107.2% at the spiked levels of 2.640, 26.40 and 100.0 μg/L with the relative standard deviations (RSDs) less than 9%. The matching degrees of the spectra for all positive samples were higher than 92%. The method is simple, accurate and efficient for the determination of sodium cyclamate in liquor and particularly suitable for confirmatory analysis of positive samples. © 2010 Editorial Office of Chinese Journal of Chromatography, Dalian Institute of Chemical Physics, CAS.