Time filter

Source Type

Jing S.-Y.,Hebei Medical University | Lu Y.-Y.,Wuhan Medical and Healthcare Center for Women and Children | Yang J.-K.,Hebei Medical University | Deng W.-Y.,Wuhan Medical and Healthcare Center for Women and Children | And 2 more authors.
European Review for Medical and Pharmacological Sciences | Year: 2016

OBJECTIVE: Long non-coding RNAs (lncRNAs) CRNDE has been identified as a tumor oncogene in glioma. However, its clinical significance and prognostic value in glioma have not been investigated until now. The aim of this study was to explore CRNDE expression levels and evaluated its clinical significance in glioma patients. PATIENTS AND METHODS: Expression levels of lncRNA CRNDE in 164 glioma specimens were determined by quantitative real-time PCR (qRT-PCR). The chi-square test was used to explore CRNDE expression with respect to clinicopathological parameters. The overall survival was analyzed by log-rank test, and survival curves were plotted according to Kaplan-Meier. Univariate and multivariate analyses were performed to analyze the prognostic significance of CRNDE expression. RESULTS: Compared with nonneoplastic brain tissues, the expression level of CRNDE was significantly increased in glioma tissues (p < 0.01). CRNDE upregulation was correlated with larger tumor size (p = 0.011), higher WHO grade (p = 0.001), and recurrence (p = 0.008). Also, survival analysis proved that up-regulated CRNDE expression was associated with poor overall survival of glioma patients (p < 0.001).The multivariate Cox regression analysis indicated that CRNDE expression was an independent prognostic factor for overall survival. CONCLUSIONS: These results indicated that lncRNA CRNDE was associated with tumor progression and could be an independent prognostic factor for glioma patients.


He X.,Wuhan Medical and Healthcare Center for Women and Children | He X.,Agency for Science, Technology and Research Singapore | Zhou A.,Wuhan Medical and Healthcare Center for Women and Children | Lu H.,Agency for Science, Technology and Research Singapore | And 7 more authors.
PLoS ONE | Year: 2013

Despite the fact that mitochondrial dysfunction has an important role in tumorigenesis and metastasis, the underlying mechanism remains to be elucidated. Mitochondrial Complex I (NADH:ubiquinone oxidoreductase) is the first and the largest protein complex of the mitochondrial electron-transport chain (ETC),which has an essential role in maintaining mitochondrial function and integrity. In this study, we separately knocked down two subunits of mitochondrial complex I, GRIM-19 or NDUFS3, and investigated their effects on metastatic behaviors and explored the possible mechanisms. Our data showed that stable down-modulation of GRIM-19 or NDUFS3 decreased complex I activity and reactive oxygen species (ROS) production; led to enhanced cell adhesion, migration, invasion, and spheroid formation; and influenced the expressions of extracellular matrix (ECM) molecules and its related proteins. We also observed that the expressions of GRIM-19, NDUFS3, and ECM elements were correlated with invasive capabilities of breast cancer cell lines. These results suggest that inhibition of complex I affects metastatic properties of cancer cells, and mitochondrial ROS might play a crucial role in these processes by regulating ECM. © 2013 He et al.


Feng L.-F.,Wuhan Medical and Healthcare Center for Women and Children | Chen X.-H.,Wuhan Medical and Healthcare Center for Women and Children | Li D.-X.,Wuhan Medical and Healthcare Center for Women and Children | Ding Y.,Wuhan Medical and Healthcare Center for Women and Children | And 3 more authors.
Chinese Journal of Contemporary Pediatrics | Year: 2016

A one-year-old girl visited the hospital due to limb torsion and developmental regression for one month after hand, foot and mouth disease. At the age of 11 months, she visited a local hospital due to fever for 5 days and skin rash with frequent convulsions for 2 days and was diagnosed with severe hand, foot and mouth disease, viral encephalitis, and status epilepticus. Brain MRI revealed symmetric abnormal signals in the bilateral basal ganglia, bilateral thalamus, cerebral peduncle, bilateral cortex, and hippocampus. She was given immunoglobulin, antiviral drugs, and anticonvulsant drugs for 2 weeks, and the effect was poor. Blood and urine screening for inherited metabolic diseases were performed to clarify the etiology. The analysis of urine organic acids showed significant increases in glutaric acid and 3-hydroxyglutaric acid, which suggested glutaric aciduria type 1, but her blood glutarylcarnitine was normal, and free carnitine significantly decreased. After the treatment with low-lysine diets, L-carnitine, and baclofen for 1 month, the patient showed a significant improvement in symptoms. Hand, foot and mouth disease is a common viral infectious disease in children, and children with underlying diseases such as inherited metabolic diseases and immunodeficiency may experience serious complications. For children with hand, foot and mouth disease and unexplained encephalopathy, inherited metabolic diseases should be considered.


Yue X.,Clinical Research Center | Zhao P.,Clinical Research Center | Wu K.,Huazhong University of Science and Technology | Huang J.,Wuhan Medical and Healthcare Center for Women and Children | And 4 more authors.
Tumor Biology | Year: 2016

Gene associated with retinoid-interferon-induced mortality (GRIM-19), an important subunit of mitochondrial complex I, has been identified as a tumor suppressor, and its reduced expression has been reported to be associated with tumorigenesis and metastasis. Autophagy has been proposed as a protective mechanism for cell survival under various stresses, including chemotherapy. However, it remains unknown whether GRIM-19 is linked to autophagy and chemotherapy resistance. Here, we showed that suppression of GRIM-19 by shRNA enhanced cell-type-dependent autophagy by activating extracellular regulated protein kinase (ERK) and hypoxia inducible factor-1a (HIF-1a) in a reactive oxygen species (ROS)-mediated manner, and thereby conferred resistance to paclitaxel. Besides, the antioxidant N-acetyl-l-cysteine (NAC) and autophagy inhibitor 3-MA could in part overcome this resistance. We also found that GRIM-19 expression was significantly correlated with clinical stage and grade in patients with cervical cancers. Taken together, our results indicated that GRIM-19 inhibition induced autophagy and chemotherapy resistance, which could affect prognosis of cervical cancers. Our study has identified new function of GRIM-19 and its underlying mechanism, and it will provide possible avenues for therapeutic targeting in cervical cancers. © 2016 International Society of Oncology and BioMarkers (ISOBM)


Peng J.,Wuhan Medical and Healthcare Center for Women and Children | Liu H.-Z.,Hubei University | Zhong J.,Hubei University | Deng Z.-F.,Wuhan Medical and Healthcare Center for Women and Children | And 10 more authors.
Oncology Reports | Year: 2016

MicroRNAs (miRNAs) are involved in the progression of different types of cancers giving new hope for cancer treatment. The role and regulatory mechanism of microRNA-187 (miR-187) are largely unknown. In the present study, 74 patients with non-small cell lung cancer (NSCLC) were selected. Tumor tissues and matched normal tissues were collected for determining the expression level of miR-187. Cell research was performed to detect the function of miR-187. The expression level was measured and miR-187 was found to be overexpressed in the NSCLC cell lines and tissues. Overexpression of miR-187 promoted cell proliferation in the A549 and H1650 cell lines. Moreover, overexpression of miR-187 also promoted cell migration and invasion. Polymerase I and transcript release factor (PTRF) was identified as a target of miR-187. Overexpression of miR-187 suppressed the expression of PTRF. Knockdown of PTRF promoted lung cancer cell invasion, and overexpression of PTRF had a negative effect on lung cancer cell invasion. The PTRF messenger RNA (mRNA) levels in cancer tissues were significantly lower than those in their adjacent normal lung tissues as determined by real-time PCR (RT-PCR). The expression of the PTRF protein was significantly weaker than that in the adjacent normal lung tissues using immunohistochemical staining. The findings revealed that miR-187 promotes cell growth and invasion by targeting PTRF and miR-187 may be a new prognostic factor for NSCLC.


Wang X.,Fudan University | Wang X.,Wuhan Medical and Healthcare Center for Women and Children | Wang H.,Fudan University | Wang H.,U.S. National Institutes of Health | And 7 more authors.
DNA and Cell Biology | Year: 2016

Resolution of the Holliday junction (HJ) is essential for homologous recombination and DNA repair. In Saccharomyces cerevisiae, HJ resolvase Yen1 and the Mus81-Mms4 complex are redundant in DNA damage repair. In cultured mammalian cells, such redundancy also exists between Yen1 ortholog GEN1 and the Mus81-Mms1 ortholog MUS81-EME1. In this report, we further tested if GEN1 and EME1 redundantly affect HJ-related physiological processes in mice. We found that combined homozygous mutations of Gen1 and Eme1 led to synthetic lethality during early embryonic stages. Homozygous Gen1 mutations did not cause DNA repair deficiency in mouse embryonic fibroblast (MEF) cells, but made heterozygous Eme1 mutant MEFs more sensitive to various DNA-damaging reagents. Gen1 mutations also reduced the meiotic recombination efficiency in Eme1 mutant mice. These results suggest that Gen1 and Eme1 play redundant roles in DNA repair and meiotic recombination in vivo. © 2016, Mary Ann Liebert, Inc.


PubMed | Wuhan Medical and Healthcare Center for Women and Children
Type: Case Reports | Journal: Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics | Year: 2016

A one-year-old girl visited the hospital due to limb torsion and developmental regression for one month after hand, foot and mouth disease. At the age of 11 months, she visited a local hospital due to fever for 5 days and skin rash with frequent convulsions for 2 days and was diagnosed with severe hand, foot and mouth disease, viral encephalitis, and status epilepticus. Brain MRI revealed symmetric abnormal signals in the bilateral basal ganglia, bilateral thalamus, cerebral peduncle, bilateral cortex, and hippocampus. She was given immunoglobulin, antiviral drugs, and anticonvulsant drugs for 2 weeks, and the effect was poor. Blood and urine screening for inherited metabolic diseases were performed to clarify the etiology. The analysis of urine organic acids showed significant increases in glutaric acid and 3-hydroxyglutaric acid, which suggested glutaric aciduria type 1, but her blood glutarylcarnitine was normal, and free carnitine significantly decreased. After the treatment with low-lysine diets, L-carnitine, and baclofen for 1 month, the patient showed a significant improvement in symptoms. Hand, foot and mouth disease is a common viral infectious disease in children, and children with underlying diseases such as inherited metabolic diseases and immunodeficiency may experience serious complications. For children with hand, foot and mouth disease and unexplained encephalopathy, inherited metabolic diseases should be considered.


PubMed | Clinical Research Center, Wuhan University, Wuhan Medical and Healthcare Center for Women and Children and Huazhong University of Science and Technology
Type: Journal Article | Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine | Year: 2016

Gene associated with retinoid-interferon-induced mortality (GRIM-19), an important subunit of mitochondrial complex I, has been identified as a tumor suppressor, and its reduced expression has been reported to be associated with tumorigenesis and metastasis. Autophagy has been proposed as a protective mechanism for cell survival under various stresses, including chemotherapy. However, it remains unknown whether GRIM-19 is linked to autophagy and chemotherapy resistance. Here, we showed that suppression of GRIM-19 by shRNA enhanced cell-type-dependent autophagy by activating extracellular regulated protein kinase (ERK) and hypoxia inducible factor-1a (HIF-1a) in a reactive oxygen species (ROS)-mediated manner, and thereby conferred resistance to paclitaxel. Besides, the antioxidant N-acetyl-L-cysteine (NAC) and autophagy inhibitor 3-MA could in part overcome this resistance. We also found that GRIM-19 expression was significantly correlated with clinical stage and grade in patients with cervical cancers. Taken together, our results indicated that GRIM-19 inhibition induced autophagy and chemotherapy resistance, which could affect prognosis of cervical cancers. Our study has identified new function of GRIM-19 and its underlying mechanism, and it will provide possible avenues for therapeutic targeting in cervical cancers.


PubMed | Hubei University and Wuhan Medical and Healthcare Center for Women and Children
Type: Journal Article | Journal: Oncology reports | Year: 2016

MicroRNAs(miRNAs) are involved in the progression of different types of cancers giving new hope for cancer treatment. The role and regulatory mechanism of microRNA187(miR187) are largely unknown. In the present study, 74 patients with nonsmall cell lung cancer(NSCLC) were selected. Tumor tissues and matched normal tissues were collected for determining the expression level of miR187. Cell research was performed to detect the function of miR187. The expression level was measured and miR187 was found to be overexpressed in the NSCLC cell lines and tissues. Overexpression of miR187 promoted cell proliferation in the A549 and H1650 cell lines. Moreover, overexpression of miR187 also promoted cell migration and invasion. PolymeraseI and transcript release factor(PTRF) was identified as a target of miR187. Overexpression of miR187 suppressed the expression of PTRF. Knockdown of PTRF promoted lung cancer cell invasion, and overexpression of PTRF had a negative effect on lung cancer cell invasion. The PTRF messenger RNA(mRNA) levels in cancer tissues were significantly lower than those in their adjacent normal lung tissues as determined by realtime PCR(RTPCR). The expression of the PTRF protein was significantly weaker than that in the adjacent normal lung tissues using immunohistochemical staining. The findings revealed that miR187 promotes cell growth and invasion by targeting PTRF and miR187 may be a new prognostic factor for NSCLC.

Loading Wuhan Medical and Healthcare Center for Women and Children collaborators
Loading Wuhan Medical and Healthcare Center for Women and Children collaborators