Time filter

Source Type

Chen C.,Wuhan University | Chen C.,Hubei Cancer Clinical Study Center | Peng J.,Wuhan University | Xia H.,Hubei Cancer Hospital | And 8 more authors.
Nanotechnology | Year: 2010

Breast cancer (BC) is a heterogeneous tumor, and better understanding of its heterogeneity is essential to improving treatment effect. Quantum dot (QD)-based immunofluorescent nanotechnology (QD-IHC) for molecular pathology has potential advantages in delineating tumor heterogeneity. This potential is explored in this paper by QD-IHC imaging of HER2 and ER. BC heterogeneity can be displayed more clearly and sensitively by QD-IHC than conventional IHC in BC tissue microarrays. Furthermore, the simultaneous imaging of ER and HER2 might help understand their interactions during the process of evolution of heterogeneous BC. © 2010 IOP Publishing Ltd.


Chen C.,Wuhan University | Chen C.,Hubei Key Laboratory of Tumor Biological Behaviors and Hubei Cancer Clinical Study Center | Xia H.-S.,Hubei Cancer Hospital | Gong Y.-P.,Hubei Cancer Hospital | And 10 more authors.
Biomaterials | Year: 2010

Accurate classification is fundamental for breast cancer (BC) personalized care. Current BC classification based on the either traditional morphological staging or molecular signatures seems inefficient to reveal the" true" behaviors of invasive BC evolution. An appropriate approach combining the macro- and micro-pathologic information might be more useful academically as well as clinically. Here we explore a holistic approach by integrating a key molecular prognostic indicator of BC, HER2, with quantitative determination using quantum dots (QDs)-based nanotechnology and spectral analysis, and a key macropathologic indicator, tumor size, resulting a new indicator, total HER2 load. This indicator might better reveal BC heterogeneity and new subtypes of BC with different 5-year disease-free survival compared with current methods, which could be helpful in formulating a more personalized targeted therapy for BC. Furthermore, this mode integrating macro- and micro-pathological indicators might help gain new insights into invasive BC biological behaviors. © 2010 Elsevier Ltd.


Chen C.,Wuhan University | Chen C.,Hubei Key Laboratory of Tumor Biological Behaviors and Hubei Cancer Clinical Study Center | Sun S.-R.,Renmin University of China | Gong Y.-P.,Hubei Cancer Hospital | And 14 more authors.
Biomaterials | Year: 2011

The emerging molecular breast cancer (BC) classification based on key molecules, including hormone receptors (HRs), and human epidermal growth factor receptor 2 (HER2) has been playing an important part of clinical practice guideline. The current molecular classification mainly based on their fingerprints, however, could not provide enough essential information for treatment decision making. The molecular information on both patterns and quantities could be more helpful to heterogeneities understanding for BC personalized medicine. Here we conduct quantitative determination of HRs and HER2 by quantum dots (QDs)-based quantitative spectral analysis, which had excellent consistence with traditional method. Moreover, we establish a new molecular classification system of BC by integrating the quantitative information of HER2 and HRs, which could better reveal BC heterogeneity and identify 5 molecular subtypes with different 5-year prognosis. Furthermore, the emerging 5 molecular subtypes based on simple quantitative molecules information could be as informative as multi-genes analysis in routine practice, and might help formulate a more personalized comprehensive therapy strategy and prognosis prediction. © 2011 Elsevier Ltd.


Peng J.,Wuhan University | Chen H.-L.,Wuhan University | Zhu X.-B.,Wuhan Jiayuan Quantum Dots Co. | Yang G.-F.,Wuhan University | And 3 more authors.
Journal of Nanoscience and Nanotechnology | Year: 2011

Quantum dots were proposed as new fluorochromes for use in fluorescence in-situ hybridization. EBV-encoded small RNA, the most abundant viral product in latently infected cells, was detected by quantum dot fluorescence in-situ hybridization in paraffin-embedded tissue sections of gastric carcinoma. An indirect FISH approach using quantum dots streptavidin conjugates as secondary reporters and digoxigenin labeled EBV-encoded small RNA oligonucleotide probes as detectable molecules was employed. Quantum dot fluorescence in-situ hybridization offered a slightly higher sensitivity in detecting EBV-encoded small RNA in gastric carcinoma than chromogenic in-situ hybridization. Statistical analyses showed that the detected EBV-associated gastric carcinoma was not associated with any clinicopathological parameters of the Chinese gastric carcinoma patients investigated in this study. © 2011 American Scientific Publishers. All rights reserved.


Peng C.-W.,Hubei University | Liu X.-L.,Hubei University | Chen C.,Hubei University | Liu X.,Hubei University | And 4 more authors.
Biomaterials | Year: 2011

Tumor growth and progression depends on their microenvironment, which undergoes constant co-evolution because of the dynamic tumor-stormal interactions. Such co-evolution has long been under appreciated due to the lack of appropriate technology platforms to simultaneously reveal these complex interactions. Here we report on a quantum dots based multiplexed imaging and spectrum analysis technology to simultaneously study major components of tumor stroma, including type IV collagen, tumor angiogenesis, macrophages infiltration and tissue destructive proteolytic enzyme matrix metalloproteinase 9. The new technology revealed a panoramic picture of the tempo-spatial co-evolution of tumor cells and their stroma at the architecture level. Four patterns of tumor invasion with distinctive co-evolution features were identified as Washing pattern, Ameba-like pattern, Polarity pattern and Linear pattern. This quantum dots based multiplexed technology could help gain new insight into the complex process of tumor invasion, and formulate new anti-cancer strategies. © 2011 Elsevier Ltd.


Chen C.,Hubei University | Chen C.,Renmin University of China | Peng J.,Wuhan University | Peng J.,Wuhan Jiayuan Quantum Dots Co. | And 4 more authors.
Nanomedicine | Year: 2012

Cancer is one of the most serious health threats worldwide. Personalized oncology holds potential for future cancer care in clinical practice, where each patient could be delivered individualized medicine on the basis of key biological features of an individual tumor. One of the most urgent problems is to develop novel approaches that incorporate the increasing molecular information into the understanding of cancer biological behaviors for personalized oncology. Quantum dots are a heterogeneous class of engineered fluorescent nanoparticles with unique optical and chemical properties, which make them promising platforms for biomedical applications. With the unique optical properties, the utilization of quantum dot-based nanotechnology has been expanded into a wide variety of attractive biomedical applications for cancer diagnosis, monitoring, pathogenesis, treatment, molecular pathology and heterogeneity in combination with cancer biomarkers. Here, we focus on the clinical application of quantum dot-based nanotechnology in personalized oncology, covering topics on individualized cancer diagnosis and treatment by in vitro and in vivo molecular imaging technologies, and in-depth understanding of the biological behaviors of tumors from a nanotechnology perspective. In addition, the major challenges in translating quantum dot-based nanotechnology into clinical application and promising future directions in personalized oncology are also discussed. © 2012 Future Medicine Ltd.


Peng C.-W.,Hubei University | Tian Q.,Hubei University | Yang G.-F.,Wuhan University | Fang M.,Hubei University | And 4 more authors.
Biomaterials | Year: 2012

Tumor microenvironment has been increasingly recognized as a complex and dynamic cancer society influencing tumor invasion and progression. The prognostic significance of this microenvironment is yet to be fully appreciated. A holistic approach to obtaining integrated information on key components in tumor microenvironment is essential. Here we reported on a quantum dots (QDs)-based simultaneous in-situ detection of infiltrating macrophages, tumor microvessels density (MVD) and neovessels maturity, in gastric cancer tissues, to obtain integrated information on these components, termed as combined tumor stromal features. These stromal features had the comparable prognostic value for overall survival, and even better prognostic value for disease-free survival, compared with traditional tumor cell-based clinico-pathological parameters. Subgroups of gastric cancer patients with favorable and unfavorable combined tumor stromal features were identified, with significantly different clinical outcomes. This study demonstrated the technical advantages of QDs-based simultaneous detection of multiple biomarkers in situ, revealed the important role of tumor stroma in cancer biology, and opened a new field to predict clinical outcome in gastric cancer from the perspectives of tumor microenvironment. © 2012 Elsevier Ltd.


Yang S.,Beijing Institute of Technology | Liu P.,Beijing Institute of Technology | Guo S.,Wuhan Jiayuan Quantum Dots Co. | Zhang L.,Beijing Institute of Technology | And 3 more authors.
Applied Physics Letters | Year: 2014

The influence of ligands on the electrical behavior of CdSe/ZnS core-shell colloidal quantum dots (CQDs)-based organic light-emitting diodes is presented. Negative differential resistance (NDR) phenomena at room temperature are observed from single-layer device ITO/poly(3,4-ethylenedioxythiophene) poly(styrenesulfonate) (PEDOT:PSS)/CQDs/Al in which the original capping ligand tri-n-octylphosphine oxide (TOPO) of CQDs is exchanged with oleylamine, as well as in both bilayer device ITO/PEDOT:PSS/CQDs/BCP(10 nm)/Al and trilayer device ITO/PEDOT:PSS/CQDs/BCP(10 nm)/Alq3(10 nm)/Al. However, such a kind of NDR phenomenon disappears if TOPO is exchanged with hexadecylamine. Therefore, NDR phenomenon depends greatly on the ligands of the CQDs, and the origin of NDR from these devices is discussed. © 2014 AIP Publishing LLC.

Loading Wuhan Jiayuan Quantum Dots Co. collaborators
Loading Wuhan Jiayuan Quantum Dots Co. collaborators