Zheng X.,Huazhong Agricultural University |
Peng K.,Huazhong Agricultural University |
Tang J.,Huazhong Agricultural University |
Wang W.,Huazhong Agricultural University |
And 9 more authors.
Journal of Animal and Veterinary Advances | Year: 2014
The aim of this study was to determine the effects of supplemental drinking boron on morphology of ostrich chicks' cerebrum. Twenty four hatched ostrich chicks were divided into six group (I-VI) and supplemented by the water with 0, 40, 80, 160, 320 and 640 mg L-1 boron, respectively for 45 days. Cerebrums were obtained and weighed after dissection then measured the transverse diameter, longitudinal diameter and height of cerebral hemisphere immediately. Paraffin embedded sections of cerebral tissues (4 μ m thick) were stained with HE, Nissl's and argentaffin and then micro photographed. It showed significant increase (p<0.05 or p<0.01) in each anatomy index of group IV in comparison with the other groups while group VT showed significant decrease. Histology study showed that neurons of the cerebrum of group I and II were alike, nerve fibers passed horizontally within the cortex. Neurons of group III were varied in size and shape and with abundance of nerve fibers passed horizontally within the cortex tighter. Whereas neurons of group IV had more types than the rest of groups and were well arranged from the edge to inside by size, the nerve fibers were rich and interweaved. In contrast, neurons of group V and VI were monomorphic with less neurite and nerve fibers were tenuous and sparse. Findings showed that 80-160 mg L-1 supplemental drinking boron promotes cerebrum development, neurons differentiation, neurite formation and nerve fibers elongation of the cerebrum of 0-45 days old ostrich chicks. © Medwell Journals, 2014.
Xiang M.,Wuhan Institute of Animal and Veterinary Science |
Lu S.,Wuhan Institute of Animal and Veterinary Science |
Gao Q.,Wuhan Institute of Animal and Veterinary Science |
Huang H.,Wuhan Institute of Animal and Veterinary Science |
And 10 more authors.
Indian Journal of Animal Sciences | Year: 2015
This study established a goat embryonic kidney (GEK) cell line which was susceptible to influenza virus. Kidney cells were separated from fetus of Matou goat and GEK cell line was established by cell biology techniques. Tumorigenicity of GEK cell line was tested by tumorigenesis assay, and its marker protein expression was tested by immunohistochemistry. Receptors of influenza virus and susceptibility to H1N1 influenza virus were also tested by immunofluorescence method and cell infection assay, respectively. Results showed that GEK cells transplanted into BALB/c nude mice could not induce tumorigenesis. Immunohistochemical analysis showed that the cells expressed cytokeratin 18 (CK-18) and vimentin which are marker proteins of renal epithelial cells. Immunofluorescent assay indicated that the abundance of α2,3-sialic acid (SA) receptor and α2,6-SA receptor on the surface of GEK cells were higher than that of Madin-Darby canine kidney (MDCK) cells. The influenza virus titers in GEK cells were similar to that of MDCK cells. Therefore, the GEK cell line established here will possibly contribute to the future influenza virus isolation and vaccines development.