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Lu Q.,Southern Medical University | Lu Q.,Wuhan General Hospital of PLA | Gong Z.,Southern Medical University | Gong Z.,Wuhan General Hospital of PLA | And 13 more authors.
International Journal of Clinical and Experimental Pathology | Year: 2017

Acute myocardial infarction (AMI) often leads to myocardial ischemia reperfusion (MIRI), thereby causing myocardial remodeling. Percutaneous coronary intervention (PCI) is the primary treatment of AMI, but easily results in myocardial ischemia-reperfusion injury, leading to myocardial remodeling. It was showed that miR-24 plays a role in cardiac remodeling. However, miR-24 expression and effect in AMI after PCI treatment have not been fully elucidated. A total of 80 cases of AMI patients after PCI surgery were enrolled. Information about echocardiography ejection fraction (EF), left ventricular end systolic diameter (LVESD), left ventricular thickness, and left ventricular end diastolic diameter (LVEDD) were recorded. MiR-24 expression before and after PCI treatment was detected by real-time PCR and analyzed with cardiac function. Rat AMI model was established and transfected by miR-24 lentivirus. Cardiac function in rats was assessed by M-mode ultrasound. Type I collagen content was determined by ELISA. Bax and Bcl-2 protein expressions in rat myocardial cells were tested by Western blot. EF reduced, LVESD and LVEDD increased, left ventricular thickness decreased, and miR-24 downregulated significantly in AMI patients after PCI compared with the preoperative group (P < 0.05). MiR-24 was positively correlated with left ventricular thickness and EF, and negatively correlated with LVESD and LVEDD (P < 0.05). Overexpression of MiR-24 in AMI rats obviously improved heart function index, reduced type I collagen content, downregulated Bax level, and enhanced Bcl-2 expression compared with AMI group (P < 0.05). MiR-24 downregulated in AMI and increased after PCI treatment. MiR-24 overexpression improved cardiac function through reducing type I collagen, regulating apoptosis balance, and alleviating MIRI.

Li M.,Union Hospital | Wang X.,Union Hospital | Fu W.,Wuhan General Hospital of PLA | He S.,Union Hospital | And 3 more authors.
Cellular Physiology and Biochemistry | Year: 2011

Objective: To investigate the regulation of CD4 +CD25 + Regulatory T cells (Tregs) on pro-inflammatory adhesion molecules, Krüppel-Like Factor-2 (KLF-2) and its downstream transcriptional targets in human umbilical vein endothelial cells (HUVECs) impaired by ox-LDL and the mechanisms of it. Methods and results: HUVECs were cultured in the continuous presence of ox-LDL(0 mg/L,25 mg/L,50 mg/L,100 mg/L) for 4, 6, 12 and 24 hours to allow identification of early-and late-induced genes, respectively, whereas non-stimulated controls were taken at 0 hours. The expression of pro-inflammatory adhesion molecules such as vascular cell adhesion molecule-1 (VCAM-1), intracellular adhesion molecule-1 (ICAM-1), E-selectin, KLF-2 and its target genes eNOS, PAI-1 were determined by real time RT-PCR and/or western-blot analysis. Expression of pro-inflammatory adhesion molecules, KLF-2, eNOS and PAI-1 in HUVEC cultured alone or with anti-CD3 mAbs activated Tregs, followed by addition of ox-LDL (50 mg/L) for 6 hours, are compared to expression levels in control cultures. Ox-LDL treated HUVECs increased pro-inflammatory adhesion molecules expression, as well as increased PAI-1 but decreased eNOS expression accompanied with significant downregulating of KLF-2 at a dose and time dependent manner. Furthermore, ox-LDL increased pro-inflammatory adhesion molecules but inhibited KLF2 expression was reversed by addition of Tregs. Small interfering RNA reduced endogenous KLF-2 expression and partly reversed the suppressive effect of Tregs on HUVECs activation, which strongly implicate KLF-2 as a transcriptional regulator of the Tregs-mediated effects in endothelial cells. Mechanism studies reveal that Treg-mediated KLF2 expression in HUVECs impaired by ox-LDL requires cell contact as well as soluble factors. Conclusions: Tregs could protect endothelial function that is largely dependent on KLF2 and its downstream transcriptional targets regulation involving cell-to-cell contact and soluble factors. Copyright © 2011 S. Karger AG, Basel.

Wei S.-J.,Wuhan General Hospital of PLA | Cai X.-H.,Wuhan General Hospital of PLA | Chen X.,Shanghai Institute of Technology | Chen D.,Shanghai Institute of Technology | Chen J.-D.,Shanghai Institute of Technology
Gongneng Cailiao yu Qijian Xuebao/Journal of Functional Materials and Devices | Year: 2012

Magnetic based capture of CTCs in microchip provides specific selection and high recovery rates with high purity. However, conventional perpendicular capture mode, which applies the magnetic field perpendicular to the flow field, shows lower capture rate under high flow field. In this paper, we suggest that the cell be captured in magnetic microchip may experience two stages which are lateral displacement stage and immobilization stage. Simulation by COMSOL and experiments were used to investigate the hydrodynamic force and magnetic force during these two stages. The data showed that the cell capture is greatly influenced by hydrodynamic force under the perpendicular capture mode. We also proposed a novel parallel capture mode design for CTC microfluidic chip. Experiment showed that this new type of chip can operated at a flow rate up to 6ml/h.

Yin Z.-G.,Chongqing Medical University | Cui M.,Chongqing Medical University | Cui M.,Wuhan General Hospital of PLA | Zhou S.-M.,Chongqing Medical University | And 3 more authors.
European Journal of Neurology | Year: 2014

Background and purpose: Metabolic syndrome (MetS) has been reported to be associated with silent lacunar infarction, which is highly related to white matter lesions (WMLs). However, little is known about the relationship between MetS and the prevalence of WMLs. The association between MetS, its components and WMLs in middle-aged and elderly patients was investigated. Methods: Consecutive patients aged 50 years and older were prospectively enrolled in this study. All participants underwent magnetic resonance imaging scans to assess the presence and severity of WMLs. The MetS was defined according to the updated National Cholesterol Education Program's Adult Treatment Panel III criteria. Multivariate logistic regression analyses were performed to examine the relationship between MetS, its components and WMLs. Results: A total of 852 patients were enrolled in the study. MetS was present in 38.4%. MetS was associated with an increased risk of periventricular WMLs (PVWMLs) and deep WMLs (DWMLs) after multivariable adjustment (odds ratio 3.21, 95% confidence interval 2.26-4.55 for PVWMLs; odds ratio 2.93, 95% confidence interval 2.09-4.09 for DWMLs). Amongst MetS components, elevated blood pressure, elevated fasting blood glucose and low high density lipoprotein cholesterol were associated with PVWMLs, whilst elevated blood pressure and low high density lipoprotein cholesterol were related to DWMLs. Conclusions: Our findings demonstrate that MetS is associated with the prevalence of PVWMLs and DWMLs independent of other risk factors in middle-aged and elderly patients. The association between MetS as a cluster and WMLs was not driven by MetS components. © 2014 EAN.

Zhang Z.-Y.,Huazhong University of Science and Technology | Chen X.-P.,Huazhong University of Science and Technology | Lu Q.-P.,Wuhan General Hospital of PLA
Frontiers of Medicine in China | Year: 2010

The inhibitory effect of different reperfusion periods 45 min following hepatic ischemia on the expression of cholecystokinin (CCK) and vasoactive intestinal peptide (VIP) in the jejunum and the effect of salvia miltiorrhiza pretreatment were investigated, and the possible mechanism and implications were explored. Eighty rats were randomly divided into four groups: normal control group (CO group), sham-operated group (SO group), ischemia/reperfusion (I/R) injury group (IR group) and salvia miltiorrhiza pretreatment group (SM group). The rat model of I/R was established by using a non-invasive artery clamp to clip (45 min) or relax the hepatic pedicle. In the SM group, saline (40 mL/kg) and salvia miltiorrhiza injection (6 g/kg) were injected via the tail vein 30 min before clipping the hepatic pedicle. In the SO group only the porta hepatis was dissected after laparotomy without clamping the hepatic pedicle. At 0, 3, 12, 24 and 72 h post-reperfusion, respectively, upper jejunum samples were taken for immunohistochemistry of CCK and VIP. It was found that 0 h after I/R, the expression of CCK and VIP in the upper jejunum was upregulated. With prolongation of the reperfusion period, the expression of CCK and VIP was also increased, reached the peak at the 24th h, and gradually returned to the normal level at the 72nd h after reperfusion. The levels of both CCK and VIP in the SM group were lower than those in the IR group. It is suggested that the digestive tract congestion injury caused by liver ischemia can upregulate the expression of CCK and VIP in the jejunum following reperfusion. Salviae pretreatment can partly reduce the increased expression of CCK and VIP in the jejunum in the same period, which might contribute to the early recovery of gastrointestinal motility. © 2010 Higher Education Press and Springer-Verlag Berlin Heidelberg.

Yin Z.-G.,Chongqing Medical University | Li L.,Chongqing Medical University | Cui M.,Wuhan General Hospital of PLA | Zhou S.-M.,Chongqing Medical University | And 2 more authors.
PLoS ONE | Year: 2014

Background: Apolipoprotein A-I (apoA-I), the major protein for high density lipoprotein, is essential for reverse cholesterol transport. Decreased serum levels of apoA-I have been reported to correlate with subcortical infarction and dementia, both of which are highly related to white matter lesions (WMLs). However, the association between apoA-I and WMLs has never been investigated. In this study, we sought to investigate the association between apoA-I and the presence of WMLs in middle-aged and elderly subjects. Methods: Consecutive patients aged 50 years and older of our department were prospectively enrolled in this study (n = 1282, 606 men and 676 women, 65.9±9.4 years). All participants underwent MRI scans to assess the presence and severity of WMLs. Multivariate logistic regression analyses were performed to examine the association of apoA-I with WMLs. Results: Patients with WMLs were older and showed significantly higher proportion of male sex, hypertension, diabetes mellitus, previous stroke, and coronary heart disease whereas levels of total cholesterol, high density lipoprotein cholesterol, and apoA-I were lower. After adjustment for potential confounders, the lowest apoA-I quartile was independently associated with an increased risk of WMLs (odds ratio: 1.87, 95% confidence interval: 1.29-2.72). In sex-specific analyses, this relationship was observed only in women. Conclusions: Our findings demonstrated that apoA-I was inversely associated with the presence of WMLs in middle-aged and elderly subjects. This results suggest that therapies which increase apoA-I concentration may be beneficial to reduce the risk of WMLs, dementia and stroke. © 2014 Yin et al.

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