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Liu J.,Chongqing Medical University | Tao X.,Chongqing Medical University | Chen L.,Wuhan General Hospital | Han W.,Capital Medical University | And 2 more authors.
Cellular Physiology and Biochemistry | Year: 2015

Background/Aims: Disordered differentiation of tendon stem cells (TSCs) during repair of injured tendon can result in the pathogenesis of chronic tendinopathy. Understanding tenocyte differentiation may provide new therapeutic insights for the prevention and treatment of chronic tendinopathy. The aim of our study was to determine if CTGF exerts a similar effect on BMP12-driven differentiation of rat TSCs. Methods: In overexpressing and RNA interference CTGF TSCs, tenogenic differentitation and the expression of related genes were determined by immunofluorescence staining, quantitative PCR, and western blotting, with or without BMP12 treatment. The interaction in vitro between CTGF and BMP12 was detected by Chemical crosslinking assay. Results: Our results showed that BMP12 effectively increased the expression of the tenocyte lineage markers scleraxis (Scx) and tenomodulin (Tnmd) at both mRNA and protein levels. Over-expression of CTGF from a lentiviral vector increased the expression of Scx and Tnmd as well as tendon proteins type I collagen (ColI) and tenascin-C (Tn-C) in TSCs compared to non-treated control cells with or without simultaneous BMP12 stimulation. Knockdown of CTGF expression decreased the expression of Scx, Tnmd, ColI and Tn-C compared to control cells. Chemical crosslinking experiments demonstrated a direct interaction between CTGF and BMP12. Conclusion: In conclusion, BMP12 plays a crucial role in tenogenesis via the Smad1/5/8 pathway, and CTGF positively promotes this effect. © 2015 S. Karger AG, Basel.

Chen J.,Xiamen University | He J.-Q.,Xiamen University | Zhen S.-Y.,Xiamen University | Huang L.-Q.,Wuhan General Hospital
Rheumatology International | Year: 2012

This study was designed to determine the effects of the osteoprotegerin (OPG) on the mRNA expression of carbonic anhydrase II (CAII) and the receptor activator of NF-jB (RANK) in mouse osteoclast-like cells.Marrow cells were harvested fromfemora and tibiae of mouse and cultured in 6-well chamber slides. After 1 day of incubation, the marrow cells were exposed to M-CSF (25 ng/ml), RANKL (50 ng/ml), and different concentrations of OPG (50, 75, and 100 ng/ml, respectively) for 3 days. Osteoclast-like cells were confirmed by both tartrate-resistant acid phosphatase (TRAP) stain and bone resorption assay. The expression of RANK and CAIImRNA was determined with real-time fluorescent quantitative polymerase chain reaction. The numbers of multinucleated, TRAP-positive osteoclast-like cells, and resorption pits formed were observed. Compared with theM-CSF + RANKLgroup,RANKmRNAexpression was statistically decreased in the M-CSF and M-CSF + RANKL + OPG(100 ng/ml) groups (P = 0.004,P = 0.024, respectively); Compared with the M-CSF, M-CSF + RANKL, and M-CSF + RANKL + OPG (100 ng/ml) group, CAIImRNA expression in the M-CSF + RANKL + OPG (75 ng/ml) groups was statistically decreased (P = 0.001, P = 0.008, and P = 0.036, respectively). These data suggest thatOPG could regulate the expression of RANK and CA II mRNA in the marrow culture system. © Springer-Verlag 2011.

Xie L.-X.,Wuhan General Hospital | Chen Y.-S.,General Hospital of Guangzhou Military Command | Liu S.-Y.,Shanghai University | Shi Y.-X.,Fudan University
Acta Radiologica | Year: 2015

Background Computed tomography (CT) findings in patients with pulmonary cryptococcosis have been reported, however, many reports were limited by the small number of patients, and not taken into account the distinction between immunocompetent and immunocompromised patients. Purpose To retrospectively evaluate thoracic CT findings in patients with pulmonary cryptococcosis whose immune status ranged from normal to severely compromised, and determine characteristic imaging features of pulmonary cryptococcosis between patients with different immune status. Material and Methods CT scan findings of 29 immunocompetent and 43 immunocompromised patients with clinically proven pulmonary cryptococcosis were reviewed retrospectively. Different patterns of CT scan abnormalities between immunocompromised and immunocompetent patients, AIDS and non-AIDS immunocompromised patients were compared by Fisher's exact test. Results Pulmonary nodules/masses, either solitary or multiple, were the most common CT finding, present in 65 (90.3%) of the 72 patients; associated findings included CT halo sign (n = 24), cavitation (n = 23), and air bronchogram (n = 17). Areas of consolidation (n = 14), areas of GGO (n = 13), linear opacities (n = 11), lymphadenopathy (n = 5), and pleural effusion (n = 8) were uncommon. The parenchymal abnormalities were peripherally located in 47 (65.2%) of the cases. Cavitations within nodules/masses were more frequently present in immunocompromised patients than in immunocompetent patients (P = 0.009), and in AIDS patients than in non-AIDS immunocompromised patients (P = 0.002). Air bronchograms within nodules/masses were more frequent present in immunocompetent patients than in immunocompromised patients (P = 0.005). Nodules/masses with halo sign were less frequent in AIDS patients than those in non-AIDS immunocompromised patients (P = 0.027). Conclusion Pulmonary cryptococcosis should be considered in the differential diagnosis of solitary or multiple pulmonary nodules. Cavitations within nodules/masses were more commonly seen in immunocompromised patients, especially AIDS patients, while air bronchograms were more commonly seen in immunocompetent patients. © The Foundation Acta Radiologica 2014.

Yan L.-R.,Wuhan General Hospital | Wu Y.-B.,Wuhan Mechanical Technology College
Proceedings - 2015 2nd International Conference on Information Science and Control Engineering, ICISCE 2015 | Year: 2015

Purpose To study the outlier detection methods and supply practical tools for data mining. Methods A novel parametric outlier detection method called maximum minimum (MaxMin) was presented to solve the crucial outlier detection issue, i.e., the estimation of the distribution parameters of the target normal distribution 'contaminated' with the outliers. Through an iterative rejection sampling procedure, a normal parametric probability density function was obtained to maximize the minimum ratio of nonparametric probability density to parametric probability density which is calculated based on each single sample. Simulation datasets, consisting of 2000 samples following the standard normal distribution contaminated by different portions of outlier following a Weibull distribution, were analysed to evaluate the performance of the MaxMin method. Results The simulation revealed that the distribution parameters could be precisely and stably estimated regardless of the proportion of the outliers in the whole dataset. The MaxMin procedure was mathematically demonstrated to be able to obtain the unbiased estimation of the distribution parameters when the outliers were not in the kernel region of the target distribution (e.g. [μ - σ, μ + σ]). Conclusion The unbiased estimation of the target distribution can be obtained by using MaxMin method under a relatively weak assumption. The outlier detection method has high precision and robustness. © 2015 IEEE.

Teng L.,University of Tokyo | Xin H.-W.,Wuhan General Hospital | Blix H.S.,Norwegian Institute of Public Health | Tsutani K.,University of Tokyo
Pharmacoepidemiology and Drug Safety | Year: 2012

Purpose: This study aimed to understand the characteristics of drug utilisation researches (DURs) using concepts of defined daily dose in China and to provide further suggestion for future DURs in China. Methods: DURs using concepts of defined daily dose published in China were identified from China Journal Full-text Database, and in-depth data analysis was conducted for DURs published in every even-numbered year. Results: In total, 2,911 DURs published between 1989 and 2009 were identified, of which 1,268 were included for further data analysis. All studies were hospital-based. Types of drugs commonly assessed in DURs were Anti-infectives for systemic use (34.1%), drugs for Nervous system (25.5%) and drugs for Alimentary tract and metabolism (14.3%). In addition, 63 DURs published in even-numbered year focusing on Chinese Herbal Medicine (CHM) were identified. Commonly used sources of defined dose were Xin Bian Yao Wu Xue/New Materia Medica (83.9%), drug information leaflets (66.8%) and Chinese Pharmacopoeia (52.0%). Common indicators used in DURs include defined daily doses (DDDs), drug utilisation index (DUI) and daily dose cost (DDC). Conclusion: DUR is a popular method to explore the use of both pharmaceutical drugs and CHM in China. The definition of defined daily dose and its related indicators presented in the DURs were highly varied. From this, it follows that DURs with more consistent methodology are highly needed for a thorough understanding of drug utilisation in China. Apart from DURs focusing on the hospital setting, more DURs from other health settings are needed. © 2012 John Wiley & Sons, Ltd.

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