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Nie S.,Wuhan Bioengineering Institute | Nie S.,Hong Kong Baptist University | Hsiao W.W.,Hong Kong Baptist University | Pan W.,Shenyang Pharmaceutical University | Yang Z.,Hong Kong Baptist University
International Journal of Nanomedicine | Year: 2011

Purpose: To develop an in situ gel system comprising liposome-containing paclitaxel (PTX) dispersed within the thermoreversible gel (Pluronic® F127 gel) for controlled release and improved antitumor drug efficiency. Methods: The dialysis membrane and membrane-less diffusion method were used to investigate the in vitro drug release behavior. Differential scanning calorimetry (DSC) thermal analysis was used to investigate the "micellization" and "sol/gel transition" process of in situ gel systems. In vitro cytotoxicity and drug uptake in KB cancer cells were determined by MTT, intercellular drug concentration, and fluorescence intensity assay. Results: The in vitro release experiment performed with a dialysis membrane model showed that the liposomal gel exhibited the longest drug-release period compared with liposome, general gel, and commercial formulation Taxol®. This effect is presumably due to the increased viscosity of liposomal gel, which has the effect of creating a drug reservoir. Both drug and gel release from the in situ gel system operated under zero-order kinetics and showed a correlation of release of PTX with gel, indicating a predominating release mechanism of the erosion type. Dispersing liposomes into the gel replaced larger gel itself for achieving the same gel dissolution rate. Both the critical micelle temperature and the sol/gel temperature, detected by DSC thermal analysis, were shifted to lower temperatures by adding liposomes. The extent of the shifts depended on the amount of embedded liposomes. MTT assay and drug uptake studies showed that the treatment with PTX-loaded liposomal 18% Pluronic F127 yielded cytotoxicities, intercellular fluorescence intensity, and drug concentration in KB cells much higher than that of conventional liposome, while blank liposomal 18% Pluronic F127 gel was far less than the Cremophor EL® vehicle and empty liposomes. Conclusions: A thermosensitive hydrogel with embedded liposome is a promising carrier for hydrophobic anticancer agents, to be used in parenteral formulations for treating local cancers. © 2011 Nie et al, publisher and licensee Dove Medical Press Ltd. Source

Wang X.,Wuhan Bioengineering Institute | Meng S.,CAS Changchun Institute of Applied Chemistry | Li D.,CAS Changchun Institute of Applied Chemistry
Separation and Purification Technology | Year: 2010

The ytterbium(III) extraction kinetics by 2-ethylhexylphosphonic acid mono-(2-ethylhexyl) ester (HEHEHP) in the presence of isooctanol have been investigated using constant interfacial cell with laminar flow. The stoichiometry of the complex formation reaction between the ytterbium(III) and HEHEHP in the presence of isooctanol had been evaluated. The extraction kinetics data had been analyzed in terms of pseudo-first order constants. From the temperature dependence of rate measurement, the values of Ea, ΔH and ΔS were calculated and it was found that the extraction process was a diffusion-controlled kinetics process with an interfacial reaction. The rate-determining step was made by predictions derived from interfacial reaction models, and through the approximate solutions of the flux equation, diffusion parameters and thickness of the diffusion film have been calculated. © 2009 Elsevier B.V. All rights reserved. Source

Wu J.-G.,Huazhong University of Science and Technology | Ge J.,Huazhong University of Science and Technology | Zhang Y.-P.,Huazhong University of Science and Technology | Yu Y.,Wuhan Bioengineering Institute | Zhang X.-Y.,Huazhong University of Science and Technology
Journal of Chemical and Engineering Data | Year: 2010

The solubility of genistein in water, methanol, ethanol, propan-2-ol, 1-butanol, and ethyl acetate was measured at temperatures ranging from (280 to 333) K under atmospheric pressure using high-performance liquid chromatography (HPLC). Ethanol could be the most suitable solvent for purification and crystallization of geinstein. The experimental data were well-correlated with the Apelblat equation, which could be used as a useful model in the purification process of genistein. © 2010 American Chemical Society. Source

Wang J.J.,Wuhan Bioengineering Institute
Advanced Materials Research | Year: 2014

With the development of digital campus network users, web users exhibit scale up, the campus network users to use different computer level each are not identical, uneven, a potential threat to network is more serious, campus network security has become an urgent need to solve the problem. In this paper, based on the neural network, the concept of Elman memory, and proposed an improved algorithm of Elman neural network, the realization of network intrusion detection. The experimental results show that, the algorithm can effectively improve the accuracy of network intrusion detection algorithm. © (2014) Trans Tech Publications, Switzerland. Source

Shi J.,Wuhan Bioengineering Institute | Wang Z.,Hubei University | Liu Y.,Hubei University | Wang C.,Hubei University
Journal of Thermal Analysis and Calorimetry | Year: 2012

The thermal behaviors of enoxacin and its hydrochloride under inert conditions were investigated by TG, FTIR, and TG/DSC-FTIR. The results indicated that the thermal behavior of enoxacin can be divided into three stages, while its hydrochloride consisted of four stages. The main difference lay in the sequence of decarboxylation and decarbonylation. For the enoxacin, the decarboxylation and decarbonylation occurred simultaneously, but for its hydrochloride, the two processes occurred in turn. The reason for this was the existence of hydrochloric acid in enoxacin hydrochloride, which changed its intermolecular force. The thermal analysis kinetic calculations of their first two stages were carried out, respectively. The apparent activation energy, pre-exponential factor, and the most probable model function were obtained using the master plots method. The results indicated that all these stages can be described by the model of nucleation and nucleus growing. © 2011 Akadémiai Kiadó, Budapest, Hungary. Source

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